Isolated human transporter proteins, nucleic acid molecules encoding human transporter proteins, and uses thereof

ABSTRACT

The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying modulators of the transporter peptides.

FIELD OF THE INVENTION

[0001] The present invention is in the field of transporter proteinsthat are related to the calcium channel transporter subfamily,recombinant DNA molecules, and protein production. The present inventionspecifically provides novel peptides and proteins that effect ligandtransport and nucleic acid molecules encoding such peptide and proteinmolecules, all of which are useful in the development of humantherapeutics and diagnostic compositions and methods.

BACKGROUND OF THE INVENTION

[0002] Transporters

[0003] Transporter proteins regulate many different functions of a cell,including cell proliferation, differentiation, and signaling processes,by regulating the flow of molecules such as ions and macromolecules,into and out of cells. Transporters are found in the plasma membranes ofvirtually every cell in eukaryotic organisms. Transporters mediate avariety of cellular functions including regulation of membranepotentials and absorption and secretion of molecules and ion across cellmembranes. When present in intracellular membranes of the Golgiapparatus and endocytic vesicles, transporters, such as chloridechannels, also regulate organelle pH. For a review, see Greger, R.(1988) Annu. Rev.

[0004] Physiol. 50:111-122.

[0005] Transporters are generally classified by structure and the typeof mode of action. In addition, transporters are sometimes classified bythe molecule type that is transported, for example, sugar transporters,chlorine channels, potassium channels, etc. There may be many classes ofchannels for transporting a single type of molecule (a detailed reviewof channel types can be found at Alexander, S. P. H. and J. A. Peters:Receptor and transporter nomenclature supplement. Trends Pharmacol.Sci., Elsevier, pp. 65-68 (1997) andhttp://www-biology.ucsd.edu/˜msaier/transport/titlepage2.html.

[0006] The following general classification scheme is known in the artand is followed in the present discoveries.

[0007] Channel-type transporters. Transmembrane channel proteins of thisclass are ubiquitously found in the membranes of all types of organismsfrom bacteria to higher eukaryotes. Transport systems of this typecatalyze facilitated diffusion (by an energy-independent process) bypassage through a transmembrane aqueous pore or channel without evidencefor a carrier-mediated mechanism. These channel proteins usually consistlargely of a-helical spanners, although b-strands may also be presentand may even comprise the channel. However, outer membrane porin-typechannel proteins are excluded from this class and are instead includedin class 9.

[0008] Carrier-type transporters. Transport systems are included in thisclass if they utilize a carrier-mediated process to catalyze uniport (asingle species is transported by facilitated diffusion), antiport (twoor more species are transported in opposite directions in a tightlycoupled process, not coupled to a direct form of energy other thanchemiosmotic energy) and/or symport (two or more species are transportedtogether in the same direction in a tightly coupled process, not coupledto a direct form of energy other than chemiosmotic energy).

[0009] Pyrophosphate bond hydrolysis-driven active transporters.Transport systems are included in this class if they hydrolyzepyrophosphate or the terminal pyrophosphate bond in ATP or anothernucleoside triphosphate to drive the active uptake and/or extrusion of asolute or solutes. The transport protein may or may not be transientlyphosphorylated, but the substrate is not phosphorylated.

[0010] PEP-dependent, phosphoryl transfer-driven group translocators.Transport systems of the bacterial phosphoenolpyruvate:sugarphosphotransferase system are included in this class. The product of thereaction, derived from extracellular sugar, is a cytoplasmicsugar-phosphate.

[0011] Decarboxylation-driven active transporters. Transport systemsthat drive solute (e.g., ion) uptake or extrusion by decarboxylation ofa cytoplasmic substrate are included in this class.

[0012] Oxidoreduction-driven active transporters. Transport systems thatdrive transport of a solute (e.g., an ion) energized by the flow ofelectrons from a reduced substrate to an oxidized substrate are includedin this class.

[0013] Light-driven active transporters. Transport systems that utilizelight energy to drive transport of a solute (e.g., an ion) are includedin this class.

[0014] Mechanically-driven active transporters. Transport systems areincluded in this class if they drive movement of a cell or organelle byallowing the flow of ions (or other solutes) through the membrane downtheir electrochemical gradients.

[0015] Outer-membrane porins (of b-structure). These proteins formtransmembrane pores or channels that usually allow the energyindependent passage of solutes across a membrane. The transmembraneportions of these proteins consist exclusively of b-strands that form ab-barrel. These porin-type proteins are found in the outer membranes ofGram-negative bacteria, mitochondria and eukaryotic plastids.

[0016] Methyltransferase-driven active transporters. A singlecharacterized protein currently falls into this category, theNa+-transporting methyltetrahydromethanopterin:coenzyme Mmethyltransferase.

[0017] Non-ribosome-synthesized channel-forming peptides or peptide-likemolecules. These molecules, usually chains of L- and D-amino acids aswell as other small molecular building blocks such as lactate, formoligomeric transmembrane ion channels. Voltage may induce channelformation by promoting assembly of the transmembrane channel. Thesepeptides are often made by bacteria and fungi as agents of biologicalwarfare.

[0018] Non-Proteinaceous Transport Complexes. Ion conducting substancesin biological membranes that do not consist of or are not derived fromproteins or peptides fall into this category.

[0019] Functionally characterized transporters for which sequence dataare lacking. Transporters of particular physiological significance willbe included in this category even though a family assignment cannot bemade.

[0020] Putative transporters in which no family member is an establishedtransporter. Putative transport protein families are grouped under thisnumber and will either be classified elsewhere when the transportfunction of a member becomes established, or will be eliminated from theTC classification system if the proposed transport function isdisproven. These families include a member or members for which atransport function has been suggested, but evidence for such a functionis not yet compelling.

[0021] Auxiliary transport proteins. Proteins that in some wayfacilitate transport across one or more biological membranes but do notthemselves participate directly in transport are included in this class.These proteins always function in conjunction with one or more transportproteins. They may provide a function connected with energy coupling totransport, play a structural role in complex formation or serve aregulatory function.

[0022] Transporters of unknown classification. Transport proteinfamilies of unknown classification are grouped under this number andwill be classified elsewhere when the transport process and energycoupling mechanism are characterized. These families include at leastone member for which a transport function has been established, buteither the mode of transport or the energy coupling mechanism is notknown.

[0023] Ion channels

[0024] An important type of transporter is the ion channel. Ion channelsregulate many different cell proliferation, differentiation, andsignaling processes by regulating the flow of ions into and out ofcells. Ion channels are found in the plasma membranes of virtually everycell in eukaryotic organisms. Ion channels mediate a variety of cellularfunctions including regulation of membrane potentials and absorption andsecretion of ion across epithelial membranes. When present inintracellular membranes of the Golgi apparatus and endocytic vesicles,ion channels, such as chloride channels, also regulate organelle pH. Fora review, see Greger, R. (1988) Annu. Rev. Physiol. 50:111-122.

[0025] Ion channels are generally classified by structure and the typeof mode of action. For example, extracellular ligand gated channels(ELGs) are comprised of five polypeptide subunits, with each subunithaving 4 membrane spanning domains, and are activated by the binding ofan extracellular ligand to the channel. In addition, channels aresometimes classified by the ion type that is transported, for example,chlorine channels, potassium channels, etc. There may be many classes ofchannels for transporting a single type of ion (a detailed review ofchannel types can be found at Alexander, S. P. H. and J. A. Peters(1997). Receptor and ion channel nomenclature supplement. TrendsPharmacol. Sci., Elsevier, pp. 65-68 andhttp://www-biology.ucsd.edu/˜msaier/transport/toc.html.

[0026] There are many types of ion channels based on structure. Forexample, many ion channels fall within one of the following groups:extracellular ligand-gated channels (ELG), intracellular ligand-gatedchannels (ILG), inward rectifying channels (INR), intercellular (gapjunction) channels, and voltage gated channels (VIC). There areadditionally recognized other channel families based on ion-typetransported, cellular location and drug sensitivity. Detailedinformation on each of these, their activity, ligand type, ion type,disease association, drugability, and other information pertinent to thepresent invention, is well known in the art.

[0027] Extracellular ligand-gated channels, ELGs, are generallycomprised of five polypeptide subunits, Unwin, N. (1993), Cell 72:31-41; Unwin, N. (1995), Nature 373: 37-43; Hucho, F., et al., (1996) J.Neurochem. 66: 1781-1792; Hucho, F., et al., (1996) Eur. J. Biochem.239: 539-557; Alexander, S. P. H. and J. A. Peters (1997), TrendsPharmacol. Sci., Elsevier, pp. 4-6; 36-40; 42-44; and Xue, H. (1998) J.Mol. Evol. 47: 323-333. Each subunit has 4 membrane spanning regions:this serves as a means of identifying other members of the ELG family ofproteins. ELG bind a ligand and in response modulate the flow of ions.Examples of ELG include most members of the neurotransmitter-receptorfamily of proteins, e.g., GABAI receptors. Other members of this familyof ion channels include glycine receptors, ryandyne receptors, andligand gated calcium channels.

[0028] The Voltage-gated Ion Channel (VIC) Superfamily

[0029] Proteins of the VIC family are ion-selective channel proteinsfound in a wide range of bacteria, archaea and eukaryotes Hille, B.(1992), Chapter 9: Structure of channel proteins; Chapter 20: Evolutionand diversity. In: Ionic Channels of Excitable Membranes, 2nd Ed.,Sinaur Assoc. Inc., Pubs., Sunderland, Mass.; Sigworth, F. J. (1993),Quart. Rev. Biophys. 27: 1-40; Salkoff, L. and T. Jegla (1995), Neuron15: 489-492; Alexander, S. P. H. et al., (1997), Trends Pharmacol. Sci.,Elsevier, pp. 76-84; Jan, L. Y. et al., (1997), Annu. Rev. Neurosci. 20:91-123; Doyle, D. A, et al., (1998) Science 280: 69-77; Terlau, H. andW. Stühmer (1998), Naturwissenschaften 85: 437-444. They are often homo-or heterooligomeric structures with several dissimilar subunits (e.g.,a1-a2-d-b Ca²⁺ channels, ab₁b₂ Na⁺ channels or (a)₄-b K⁺ channels), butthe channel and the primary receptor is usually associated with the a(or a1) subunit. Functionally characterized members are specific for K⁺,Na⁺ or Ca²⁺. The K⁺ channels usually consist of homotetramericstructures with each a-subunit possessing six transmembrane spanners(TMSs). The a1 and a subunits of the Ca²⁺ and Na⁺ channels,respectively, are about four times as large and possess 4 units, eachwith 6 TMSs separated by a hydrophilic loop, for a total of 24 TMSs.These large channel proteins form heterotetra-unit structures equivalentto the homotetrameric structures of most K⁺ channels. All four units ofthe Ca²⁺ and Na⁺ channels are homologous to the single unit in thehomotetrameric K⁺ channels. Ion flux via the eukaryotic channels isgenerally controlled by the transmembrane electrical potential (hencethe designation, voltage-sensitive) although some are controlled byligand or receptor binding.

[0030] Several putative K⁺-selective channel proteins of the VIC familyhave been identified in prokaryotes. The structure of one of them, theKcsA K⁺ channel of Streptomyces lividans, has been solved to 3.2 Åresolution. The protein possesses four identical subunits, each with twotransmembrane helices, arranged in the shape of an inverted teepee orcone. The cone cradles the “selectivity filter” P domain in its outerend. The narrow selectivity filter is only 12 Å long, whereas theremainder of the channel is wider and lined with hydrophobic residues. Alarge water-filled cavity and helix dipoles stabilize K⁺ in the pore.The selectivity filter has two bound K⁺ ions about 7.5 Å apart from eachother. Ion conduction is proposed to result from a balance ofelectrostatic attractive and repulsive forces.

[0031] In eukaryotes, each VIC family channel type has several subtypesbased on pharmacological and electrophysiological data. Thus, there arefive types of Ca²⁺ channels (L, N, P, Q and T). There are at least tentypes of K⁺ channels, each responding in different ways to differentstimuli: voltage-sensitive [Ka, Kv, Kvr, Kvs and Ksr], Ca²⁺-sensitive[BK_(Ca), IK_(Ca) and SK_(Ca)] and receptor-coupled [K_(M) and K_(ACh)].There are at least six types of Na⁺ channels (I, II, III, μ1, H1 andPN3). Tetrameric channels from both prokaryotic and eukaryotic organismsare known in which each a-subunit possesses 2 TMSs rather than 6, andthese two TMSs are homologous to TMSs 5 and 6 of the six TMS unit foundin the voltage-sensitive channel proteins. KcsA of S. lividans is anexample of such a 2 TMS channel protein. These channels may include theKNa (Na⁺-activated) and K_(Vol) (cell volume-sensitive) K⁺ channels, aswell as distantly related channels such as the Tok1 K⁺ channel of yeast,the TWIK-1 inward rectifier K⁺ channel of the mouse and the TREK-1 K⁺channel of the mouse. Because of insufficient sequence similarity withproteins of the VIC family, inward rectifier K⁺ IRK channels(ATP-regulated; G-protein-activated) which possess a P domain and twoflanking TMSs are placed in a distinct family. However, substantialsequence similarity in the P region suggests that they are homologous.The b, g and d subunits of VIC family members, when present, frequentlyplay regulatory roles in channel activation/deactivation.

[0032] Experimental evidence indicates that voltage gated Ca²⁺ channelsmay be implicated in diseases such as Lambert-Eaton myasthenic syndrome,a paraneoplastic neuromuscular disorder in which an autoimmune responsedirected against a small-cell lung tumor crossreacts with antigens inthe neuromuscular junction. For more information, see Rosenfeld, M. R.,et al., Ann. Neurol. 33: 113-120, 1993, PubMed ID: 8494331; and Taviaux,S., et al., Hum. Genet. 100: 151-154, 1997, PubMed ID: 9254841.

[0033] The Epithelial Na⁺ Channel (ENaC) Family

[0034] The ENaC family consists of over twenty-four sequenced proteins(Canessa, C. M., et al., (1994), Nature 367: 463-467, Le, T. and M. H.Saier, Jr. (1996), Mol. Membr. Biol. 13: 149-157; Garty, H. and L. G.Palmer (1997), Physiol. Rev. 77: 359-396; Waldmann, R., et al., (1997),Nature 386: 173-177; Darboux, I., et al., (1998), J. Biol. Chem. 273:9424-9429; Firsov, D., et al., (1998), EMBO J. 17: 344-352; Horisberger,J. -D. (1998). Curr. Opin. Struc. Biol. 10: 443-449). All are fromanimals with no recognizable homologues in other eukaryotes or bacteria.The vertebrate ENaC proteins from epithelial cells cluster tightlytogether on the phylogenetic tree: voltage-insensitive ENaC homologuesare also found in the brain. Eleven sequenced C. elegans proteins,including the degenerins, are distantly related to the vertebrateproteins as well as to each other. At least some of these proteins formpart of a mechano-transducing complex for touch sensitivity. Thehomologous Helix aspersa (FMRF-amide)-activated Na⁺ channel is the firstpeptide neurotransmitter-gated ionotropic receptor to be sequenced.

[0035] Protein members of this family all exhibit the same apparenttopology, each with N- and C-termini on the inside of the cell, twoamphipathic transmembrane spanning segments, and a large extracellularloop. The extracellular domains contain numerous highly conservedcysteine residues. They are proposed to serve a receptor function.

[0036] Mammalian ENaC is important for the maintenance of Na⁺ balanceand the regulation of blood pressure. Three homologous ENaC subunits,alpha, beta, and gamma, have been shown to assemble to form the highlyNa⁺-selective channel. The stoichiometry of the three subunits isalpha₂, betal, gammal in a heterotetrameric architecture.

[0037] The Glutamate-gated Ion Channel (GIC) Family of NeurotransmitterReceptors Members of the GIC family are heteropentameric complexes inwhich each of the subunits is of 800-1000 amino acyl residues in length(Nakanishi, N., et al, (1990), Neuron 5: 569-581; Unwin, N. (1993), Cell72: 31-41; Alexander, S. P. H. and J. A. Peters (1997) Trends Pharmacol.Sci., Elsevier, pp. 36-40). These subunits may span the membrane threeor five times as putative a-helices with the N-termini (theglutamate-binding domains) localized extracellularly and the C-terminilocalized cytoplasmically. They may be distantly related to theligand-gated ion channels, and if so, they may possess substantialb-structure in their transmembrane regions. However, homology betweenthese two families cannot be established on the basis of sequencecomparisons alone. The subunits fall into six subfamilies: a, b, g, d, eand z.

[0038] The GIC channels are divided into three types: (1)a-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-, (2) kainate-and (3) N-methyl-D-aspartate (NMDA)-selective glutamate receptors.Subunits of the AMPA and kainate classes exhibit 35-40% identity witheach other while subunits of the NMDA receptors exhibit 22-24% identitywith the former subunits. They possess large N-terminal, extracellularglutamate-binding domains that are homologous to the periplasmicglutamine and glutamate receptors of ABC-type uptake permeases ofGram-negative bacteria. All known members of the GIC family are fromanimals. The different channel (receptor) types exhibit distinct ionselectivities and conductance properties. The NMDA-selective largeconductance channels are highly permeable to monovalent cations andCa²⁺. The AMPA- and kainate-selective ion channels are permeableprimarily to monovalent cations with only low permeability to Ca⁺.

[0039] The Chloride Channel (ClC) Family

[0040] The ClC family is a large family consisting of dozens ofsequenced proteins derived from Gram-negative and Gram-positivebacteria, cyanobacteria, archaea, yeast, plants and animals (Steinmeyer,K., et al., (1991), Nature 354: 301-304; Uchida, S., et al., (1993), J.Biol. Chem. 268: 3821-3824; Huang, M. -E., et al., (1994), J. Mol. Biol.242: 595-598; Kawasaki, M., et al, (1994), Neuron 12: 597-604; Fisher,W. E., et al., (1995), Genomics. 29:598-606; and Foskett, J. K. (1998),Annu. Rev. Physiol. 60: 689-717). These proteins are essentiallyubiquitous, although they are not encoded within genomes of Haemophilusinfluenzae, Mycoplasma genitalium, and Mycoplasma pneumoniae. Sequencedproteins vary in size from 395 amino acyl residues (M. jannaschii) to988 residues (man). Several organisms contain multiple ClC familyparalogues. For example, Synechocystis has two paralogues, one of 451residues in length and the other of 899 residues. Arabidopsis thalianahas at least four sequenced paralogues, (775-792 residues), humans alsohave at least five paralogues (820-988 residues), and C. elegans alsohas at least five (810-950 residues). There are nine known members inmammals, and mutations in three of the corresponding genes cause humandiseases. E. coli, Methanococcus jannaschii and Saccharomyces cerevisiaeonly have one ClC family member each. With the exception of the largerSynechocystis paralogue, all bacterial proteins are small (395-492residues) while all eukaryotic proteins are larger (687-988 residues).These proteins exhibit 10-12 putative transmembrane a-helical spanners(TMSs) and appear to be present in the membrane as homodimers. While onemember of the family, Torpedo ClC-O, has been reported to have twochannels, one per subunit, others are believed to have just one.

[0041] All functionally characterized members of the ClC familytransport chloride, some in a voltage-regulated process. These channelsserve a variety of physiological functions (cell volume regulation;membrane potential stabilization; signal transduction; transepithelialtransport, etc.). Different homologues in humans exhibit differing anionselectivities, i.e., ClC4 and ClC5 share a NO₃ ⁻>Cl⁻>Br⁻>I⁻ conductancesequence, while ClC3 has an I⁻>Cl⁻ selectivity. The ClC4 and ClC5channels and others exhibit outward rectifying currents with currentsonly at voltages more positive than +20 mV.

[0042] Animal Inward Rectifier K⁺ Channel (IRK-C) Family

[0043] IRK channels possess the “minimal channel-forming structure” withonly a P domain, characteristic of the channel proteins of the VICfamily, and two flanking transmembrane spanners (Shuck, M. E., et al.,(1994), J. Biol. Chem. 269: 24261-24270; Ashen, M. D., et al., (1995),Am. J. Physiol. 268: H506-H511; Salkoff, L. and T. Jegla (1995), Neuron15: 489-492; Aguilar-Bryan, L., et al., (1998), Physiol. Rev. 78:227-245; Ruknudin, A., et al., (1998), J. Biol. Chem. 273: 14165-14171).They may exist in the membrane as homo- or heterooligomers. They have agreater tendency to let K⁺ flow into the cell than out.Voltage-dependence may be regulated by external K⁺, by internal Mg²⁺, byinternal ATP and/or by G-proteins. The P domains of IRK channels exhibitlimited sequence similarity to those of the VIC family, but thissequence similarity is insufficient to establish homology. Inwardrectifiers play a role in setting cellular membrane potentials, and theclosing of these channels upon depolarization permits the occurrence oflong duration action potentials with a plateau phase. Inward rectifierslack the intrinsic voltage sensing helices found in VIC family channels.In a few cases, those of Kir1.1a and Kir6.2, for example, directinteraction with a member of the ABC superfamily has been proposed toconfer unique functional and regulatory properties to the heteromericcomplex, including sensitivity to ATP. The SUR1 sulfonylurea receptor(spQ09428) is the ABC protein that regulates the Kir6.2 channel inresponse to ATP, and CFTR may regulate Kir1.1a. Mutations in SUR1 arethe cause of familial persistent hyperinsulinemic hypoglycemia ininfancy (PHHI), an autosomal recessive disorder characterized byunregulated insulin secretion in the pancreas.

[0044] ATP-gated Cation Channel (ACC) Family

[0045] Members of the ACC family (also called P2X receptors) respond toATP, a functional neurotransmitter released by exocytosis from manytypes of neurons (North, R. A. (1996), Curr. Opin. Cell Biol. 8:474-483; Soto, F., M. Garcia-Guzman and W. Stühmer (1997), J. Membr.Biol. 160: 91-100). They have been placed into seven groups (P2X₁-P2X₇)based on their pharmacological properties. These channels, whichfunction at neuron-neuron and neuron-smooth muscle junctions, may playroles in the control of blood pressure and pain sensation. They may alsofunction in lymphocyte and platelet physiology. They are found only inanimals.

[0046] The proteins of the ACC family are quite similar in sequence(>35% identity), but they possess 380-1000 amino acyl residues persubunit with variability in length localized primarily to the C-terminaldomains. They possess two transmembrane spanners, one about 30-50residues from their N-termini, the other near residues 320-340. Theextracellular receptor domains between these two spanners (of about 270residues) are well conserved with numerous conserved glycyl and cysteylresidues. The hydrophilic C-termini vary in length from 25 to 240residues. They resemble the topologically similar epithelial Na⁺ channel(ENaC) proteins in possessing (a) N- and C-termini localizedintracellularly, (b) two putative transmembrane spanners, (c) a largeextracellular loop domain, and (d) many conserved extracellular cysteylresidues. ACC family members are, however, not demonstrably homologouswith them. ACC channels are probably hetero- or homomultimers andtransport small monovalent cations (Me⁺). Some also transport Ca²⁺; afew also transport small metabolites.

[0047] The Ryanodine-Inositol 1,4,5-triphosphate Receptor Ca²⁺ Channel(RIR-CaC) Family

[0048] Ryanodine (Ry)-sensitive and inositol 1,4,5-triphosphate(IP3)-sensitive Ca²⁺-release channels function in the release of Ca²⁺from intracellular storage sites in animal cells and thereby regulatevarious Ca²⁺-dependent physiological processes (Hasan, G. et al., (1992)Development 116: 967-975; Michikawa, T., et al., (1994), J. Biol. Chem.269: 9184-9189; Tunwell, R. E. A., (1996), Biochem. J. 318: 477-487;Lee, A. G. (1996) Biomembranes, Vol. 6, Transmembrane Receptors andChannels (A. G. Lee, ed.), JAI Press, Denver, Colo., pp 291-326;Mikoshiba, K., et al., (1996) J. Biochem. Biomem. 6: 273-289). Ryreceptors occur primarily in muscle cell sarcoplasmic reticular (SR)membranes, and IP3 receptors occur primarily in brain cell endoplasmicreticular (ER) membranes where they effect release of Ca²⁺ into thecytoplasm upon activation (opening) of the channel.

[0049] The Ry receptors are activated as a result of the activity ofdihydropyridine-sensitive Ca²⁺ channels. The latter are members of thevoltage-sensitive ion channel (VIC) family. Dihydropyridine-sensitivechannels are present in the T-tubular systems of muscle tissues.

[0050] Ry receptors are homotetrameric complexes with each subunitexhibiting a molecular size of over 500,000 daltons (about 5,000 aminoacyl residues). They possess C-terminal domains with six putativetransmembrane a -helical spanners (TMSs). Putative pore-formingsequences occur between the fifth and sixth TMSs as suggested formembers of the VIC family. The large N-terminal hydrophilic domains andthe small C-terminal hydrophilic domains are localized to the cytoplasm.Low resolution 3-dimensional structural data are available. Mammalspossess at least three isoforms that probably arose by gene duplicationand divergence before divergence of the mammalian species. Homologuesare present in humans and Caenorabditis elegans.

[0051] IP₃ receptors resemble Ry receptors in many respects. (1) Theyare homotetrameric complexes with each subunit exhibiting a molecularsize of over 300,000 daltons (about 2,700 amino acyl residues). (2) Theypossess C-terminal channel domains that are homologous to those of theRy receptors. (3) The channel domains possess six putative TMSs and aputative channel lining region between TMSs 5 and 6. (4) Both the largeN-terminal domains and the smaller C-terminal tails face the cytoplasm.(5) They possess covalently linked carbohydrate on extracytoplasmicloops of the channel domains. (6) They have three currently recognizedisoforms (types 1, 2, and 3) in mammals which are subject todifferential regulation and have different tissue distributions.

[0052] IP₃ receptors possess three domains: N-terminal IP₃-bindingdomains, central coupling or regulatory domains and C-terminal channeldomains. Channels are activated by IP₃ binding, and like the Ryreceptors, the activities of the IP₃ receptor channels are regulated byphosphorylation of the regulatory domains, catalyzed by various proteinkinases. They predominate in the endoplasmic reticular membranes ofvarious cell types in the brain but have also been found in the plasmamembranes of some nerve cells derived from a variety of tissues.

[0053] The channel domains of the Ry and IP₃ receptors comprise acoherent family that in spite of apparent structural similarities, donot show appreciable sequence similarity of the proteins of the VICfamily. The Ry receptors and the IP₃ receptors cluster separately on theRIR-CaC family tree. They both have homologues in Drosophila. Based onthe phylogenetic tree for the family, the family probably evolved in thefollowing sequence: (1) A gene duplication event occurred that gave riseto Ry and IP₃ receptors in invertebrates. (2) Vertebrates evolved frominvertebrates. (3) The three isoforms of each receptor arose as a resultof two distinct gene duplication events. (4) These isoforms weretransmitted to mammals before divergence of the mammalian species.

[0054] The Organellar Chloride Channel (O-ClC) Family

[0055] Proteins of the O-ClC family are voltage-sensitive chloridechannels found in intracellular membranes but not the plasma membranesof animal cells (Landry, D, et al., (1993), J. Biol. Chem. 268:14948-14955; Valenzuela, Set al., (1997), J. Biol. Chem. 272:12575-12582; and Duncan, R. R., et al., (1997), J. Biol. Chem. 272:23880-23886).

[0056] They are found in human nuclear membranes, and the bovine proteintargets to the microsomes, but not the plasma membrane, when expressedin Xenopus laevis oocytes. These proteins are thought to function in theregulation of the membrane potential and in transepithelial ionabsorption and secretion in the kidney. They possess two putativetransmembrane a-helical spanners (TMSs) with cytoplasmic N- andC-termini and a large luminal loop that may be glycosylated. The bovineprotein is 437 amino acyl residues in length and has the two putativeTMSs at positions 223-239 and 367-385. The human nuclear protein is muchsmaller (241 residues). A C. elegans homologue is 260 residues long.

[0057] Transporter proteins, particularly members of the calcium channelsubfamily, are a major target for drug action and development.Accordingly, it is valuable to the field of pharmaceutical developmentto identify and characterize previously unknown transport proteins. Thepresent invention advances the state of the art by providing previouslyunidentified human transport proteins.

SUMMARY OF THE INVENTION

[0058] The present invention is based in part on the identification ofamino acid sequences of human transporter peptides and proteins that arerelated to the calcium channel transporter subfamily, as well as allelicvariants and other mammalian orthologs thereof. These unique peptidesequences, and nucleic acid sequences that encode these peptides, can beused as models for the development of human therapeutic targets, aid inthe identification of therapeutic proteins, and serve as targets for thedevelopment of human therapeutic agents that modulate transporteractivity in cells and tissues that express the transporter. Experimentaldata as provided in FIG. 1 indicates expression in humans in the liver,adrenal gland, normal and tumorous nervous tissue, adult amygdala, brainmeningioma tissue, denis-drash, adult and fetal brain, placenta, testisand kidney.

DESCRIPTION OF THE FIGURE SHEETS

[0059]FIG. 1 provides the nucleotide sequence of a cDNA molecule ortranscript sequence that encodes the transporter protein of the presentinvention. (SEQ ID NO:1) In addition structure and functionalinformation is provided, such as ATG start, stop and tissuedistribution, where available, that allows one to readily determinespecific uses of inventions based on this molecular sequence.Experimental data as provided in FIG. 1 indicates expression in humansin the liver, adrenal gland, normal and tumorous nervous tissue, adultamygdala, brain meningioma tissue, denis-drash, adult and fetal brain,placenta, testis and kidney.

[0060]FIG. 2 provides the predicted amino acid sequence of thetransporter of the present invention. (SEQ ID NO:2) In additionstructure and functional information such as protein family, function,and modification sites is provided where available, allowing one toreadily determine specific uses of inventions based on this molecularsequence.

[0061]FIG. 3 provides genomic sequences that span the gene encoding thetransporter protein of the present invention. (SEQ ID NO:3) In additionstructure and functional information, such as intron/exon structure,promoter location, etc., is provided where available, allowing one toreadily determine specific uses of inventions based on this molecularsequence. As illustrated in FIG. 3, SNPs were identified at 47 differentnucleotide positions.

DETAILED DESCRIPTION OF THE INVENTION

[0062] General Description

[0063] The present invention is based on the sequencing of the humangenome. During the sequencing and assembly of the human genome, analysisof the sequence information revealed previously unidentified fragmentsof the human genome that encode peptides that share structural and/orsequence homology to protein/peptide/domains identified andcharacterized within the art as being a transporter protein or part of atransporter protein and are related to the calcium channel transportersubfamily. Utilizing these sequences, additional genomic sequences wereassembled and transcript and/or cDNA sequences were isolated andcharacterized. Based on this analysis, the present invention providesamino acid sequences of human transporter peptides and proteins that arerelated to the calcium channel transporter subfamily, nucleic acidsequences in the form of transcript sequences, cDNA sequences and/orgenomic sequences that encode these transporter peptides and proteins,nucleic acid variation (allelic information), tissue distribution ofexpression, and information about the closest art knownprotein/peptide/domain that has structural or sequence homology to thetransporter of the present invention.

[0064] In addition to being previously unknown, the peptides that areprovided in the present invention are selected based on their ability tobe used for the development of commercially important products andservices. Specifically, the present peptides are selected based onhomology and/or structural relatedness to known transporter proteins ofthe calcium channel transporter subfamily and the expression patternobserved. Experimental data as provided in FIG. 1 indicates expressionin humans in the liver, adrenal gland, normal and tumorous nervoustissue, adult amygdala, brain meningioma tissue, denis-drash, adult andfetal brain, placenta, testis and kidney.. The art has clearlyestablished the commercial importance of members of this family ofproteins and proteins that have expression patterns similar to that ofthe present gene. Some of the more specific features of the peptides ofthe present invention, and the uses thereof, are described herein,particularly in the Background of the Invention and in the annotationprovided in the Figures, and/or are known within the art for each of theknown calcium channel family or subfamily of transporter proteins.

[0065] Specific Embodiments

[0066] Peptide Molecules

[0067] The present invention provides nucleic acid sequences that encodeprotein molecules that have been identified as being members of thetransporter family of proteins and are related to the calcium channeltransporter subfamily (protein sequences are provided in FIG. 2,transcript/cDNA sequences are provided in FIGS. 1 and genomic sequencesare provided in FIG. 3). The peptide sequences provided in FIG. 2, aswell as the obvious variants described herein, particularly allelicvariants as identified herein and using the information in FIG. 3, willbe referred herein as the transporter peptides of the present invention,transporter peptides, or peptides/proteins of the present invention.

[0068] The present invention provides isolated peptide and proteinmolecules that consist of, consist essentially of, or comprising theamino acid sequences of the transporter peptides disclosed in the FIG.2, (encoded by the nucleic acid molecule shown in FIG. 1,transcript/cDNA or FIG. 3, genomic sequence), as well as all obviousvariants of these peptides that are within the art to make and use. Someof these variants are described in detail below.

[0069] As used herein, a peptide is said to be “isolated” or “purified”when it is substantially free of cellular material or free of chemicalprecursors or other chemicals. The peptides of the present invention canbe purified to homogeneity or other degrees of purity. The level ofpurification will be based on the intended use. The critical feature isthat the preparation allows for the desired function of the peptide,even if in the presence of considerable amounts of other components (thefeatures of an isolated nucleic acid molecule is discussed below).

[0070] In some uses, “substantially free of cellular material” includespreparations of the peptide having less than about 30% (by dry weight)other proteins (i.e., contaminating protein), less than about 20% otherproteins, less than about 10% other proteins, or less than about 5%other proteins. When the peptide is recombinantly produced, it can alsobe substantially free of culture medium, i.e., culture medium representsless than about 20% of the volume of the protein preparation.

[0071] The language “substantially free of chemical precursors or otherchemicals” includes preparations of the peptide in which it is separatedfrom chemical precursors or other chemicals that are involved in itssynthesis. In one embodiment, the language “substantially free ofchemical precursors or other chemicals” includes preparations of thetransporter peptide having less than about 30% (by dry weight) chemicalprecursors or other chemicals, less than about 20% chemical precursorsor other chemicals, less than about 10% chemical precursors or otherchemicals, or less than about 5% chemical precursors or other chemicals.

[0072] The isolated transporter peptide can be purified from cells thatnaturally express it, purified from cells that have been altered toexpress it (recombinant), or synthesized using known protein synthesismethods. Experimental data as provided in FIG. 1 indicates expression inhumans in the liver, adrenal gland, normal and tumorous nervous tissue,adult amygdala, brain meningioma tissue, denis-drash, adult and fetalbrain, placenta, testis and kidney. For example, a nucleic acid moleculeencoding the transporter peptide is cloned into an expression vector,the expression vector introduced into a host cell and the proteinexpressed in the host cell. The protein can then be isolated from thecells by an appropriate purification scheme using standard proteinpurification techniques. Many of these techniques are described indetail below.

[0073] Accordingly, the present invention provides proteins that consistof the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), forexample, proteins encoded by the transcript/cDNA nucleic acid sequencesshown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG.3 (SEQ ID NO:3). The amino acid sequence of such a protein is providedin FIG. 2. A protein consists of an amino acid sequence when the aminoacid sequence is the final amino acid sequence of the protein.

[0074] The present invention further provides proteins that consistessentially of the amino acid sequences provided in FIG. 2 (SEQ IDNO:2), for example, proteins encoded by the transcript/cDNA nucleic acidsequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequencesprovided in FIG. 3 (SEQ ID NO:3). A protein consists essentially of anamino acid sequence when such an amino acid sequence is present withonly a few additional amino acid residues, for example from about 1 toabout 100 or so additional residues, typically from 1 to about 20additional residues in the final protein.

[0075] The present invention further provides proteins that comprise theamino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example,proteins encoded by the transcript/cDNA nucleic acid sequences shown inFIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQID NO:3). A protein comprises an amino acid sequence when the amino acidsequence is at least part of the final amino acid sequence of theprotein. In such a fashion, the protein can be only the peptide or haveadditional amino acid molecules, such as amino acid residues (contiguousencoded sequence) that are naturally associated with it or heterologousamino acid residues/peptide sequences. Such a protein can have a fewadditional amino acid residues or can comprise several hundred or moreadditional amino acids. The preferred classes of proteins that arecomprised of the transporter peptides of the present invention are thenaturally occurring mature proteins. A brief description of how varioustypes of these proteins can be made/isolated is provided below.

[0076] The transporter peptides of the present invention can be attachedto heterologous sequences to form chimeric or fusion proteins. Suchchimeric and fusion proteins comprise a transporter peptide operativelylinked to a heterologous protein having an amino acid sequence notsubstantially homologous to the transporter peptide. “Operativelylinked” indicates that the transporter peptide and the heterologousprotein are fused in-frame. The heterologous protein can be fused to theN-terminus or C-terminus of the transporter peptide.

[0077] In some uses, the fusion protein does not affect the activity ofthe transporter peptide per se. For example, the fusion protein caninclude, but is not limited to, enzymatic fusion proteins, for examplebeta-galactosidase fusions, yeast two-hybrid GAL fusions, poly-Hisfusions, MYC-tagged, HI-tagged and Ig fusions. Such fusion proteins,particularly poly-His fusions, can facilitate the purification ofrecombinant transporter peptide. In certain host cells (e.g., mammalianhost cells), expression and/or secretion of a protein can be increasedby using a heterologous signal sequence.

[0078] A chimeric or fusion protein can be produced by standardrecombinant DNA techniques. For example, DNA fragments coding for thedifferent protein sequences are ligated together in-frame in accordancewith conventional techniques. In another embodiment, the fusion gene canbe synthesized by conventional techniques including automated DNAsynthesizers. Alternatively, PCR amplification of gene fragments can becarried out using anchor primers which give rise to complementaryoverhangs between two consecutive gene fragments which can subsequentlybe annealed and re-amplified to generate a chimeric gene sequence (seeAusubel et al., Current Protocols in Molecular Biology, 1992). Moreover,many expression vectors are commercially available that already encode afusion moiety (e.g., a GST protein). A transporter peptide-encodingnucleic acid can be cloned into such an expression vector such that thefusion moiety is linked in-frame to the transporter peptide.

[0079] As mentioned above, the present invention also provides andenables obvious variants of the amino acid sequence of the proteins ofthe present invention, such as naturally occurring mature forms of thepeptide, allelic/sequence variants of the peptides, non-naturallyoccurring recombinantly derived variants of the peptides, and orthologsand paralogs of the peptides. Such variants can readily be generatedusing art-known techniques in the fields of recombinant nucleic acidtechnology and protein biochemistry. It is understood, however, thatvariants exclude any amino acid sequences disclosed prior to theinvention.

[0080] Such variants can readily be identified/made using moleculartechniques and the sequence information disclosed herein. Further, suchvariants can readily be distinguished from other peptides based onsequence and/or structural homology to the transporter peptides of thepresent invention. The degree of homology/identity present will be basedprimarily on whether the peptide is a functional variant ornon-functional variant, the amount of divergence present in the paralogfamily and the evolutionary distance between the orthologs.

[0081] To determine the percent identity of two amino acid sequences ortwo nucleic acid sequences, the sequences are aligned for optimalcomparison purposes (e.g., gaps can be introduced in one or both of afirst and a second amino acid or nucleic acid sequence for optimalalignment and non-homologous sequences can be disregarded for comparisonpurposes). In a preferred embodiment, at least 30%, 40%, 50%, 60%, 70%,80%, or 90% or more of a reference sequence is aligned for comparisonpurposes. The amino acid residues or nucleotides at corresponding aminoacid positions or nucleotide positions are then compared. When aposition in the first sequence is occupied by the same amino acidresidue or nucleotide as the corresponding position in the secondsequence, then the molecules are identical at that position (as usedherein amino acid or nucleic acid “identity” is equivalent to amino acidor nucleic acid “homology”). The percent identity between the twosequences is a function of the number of identical positions shared bythe sequences, taking into account the number of gaps, and the length ofeach gap, which need to be introduced for optimal alignment of the twosequences.

[0082] The comparison of sequences and determination of percent identityand similarity between two sequences can be accomplished using amathematical algorithm. (Computational Molecular Biology, Lesk, A. M.,ed., Oxford University Press, New York, 1988; Biocomputing: Informaticsand Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993;Computer Analysis of Sequence Data, Part 1, Griffin, A. M., and Griffin,H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis inMolecular Biology, von Heinje, G., Academic Press, 1987; and SequenceAnalysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press,New York, 1991). In a preferred embodiment, the percent identity betweentwo amino acid sequences is determined using the Needleman and Wunsch(J. Mol. Biol. (48):444-453 (1970)) algorithm which has beenincorporated into the GAP program in the GCG software package (availableat http://www.gcg.com), using either a Blossom 62 matrix or a PAM250matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a lengthweight of 1, 2, 3, 4, 5, or 6. In yet another preferred embodiment, thepercent identity between two nucleotide sequences is determined usingthe GAP program in the GCG software package (Devereux, J., et al.,Nucleic Acids Res. 12(1):387 (1984)) (available at http://www.gcg.com),using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80and a length weight of 1, 2, 3, 4, 5, or 6. In another embodiment, thepercent identity between two amino acid or nucleotide sequences isdetermined using the algorithm of E. Myers and W. Miller (CABIOS,4:11-17 (1989)) which has been incorporated into the ALIGN program(version 2.0), using a PAM120 weight residue table, a gap length penaltyof 12 and a gap penalty of 4.

[0083] The nucleic acid and protein sequences of the present inventioncan further be used as a “query sequence” to perform a search againstsequence databases to, for example, identify other family members orrelated sequences. Such searches can be performed using the NBLAST andXBLAST programs (version 2.0) of Altschul, et al. (J. Mol. Biol.215:403-10 (1990)). BLAST nucleotide searches can be performed with theNBLAST program, score=100, wordlength=12 to obtain nucleotide sequenceshomologous to the nucleic acid molecules of the invention. BLAST proteinsearches can be performed with the XBLAST program, score=50,wordlength=3 to obtain amino acid sequences homologous to the proteinsof the invention. To obtain gapped alignments for comparison purposes,Gapped BLAST can be utilized as described in Altschul et al. (NucleicAcids Res. 25(17):3389-3402 (1997)). When utilizing BLAST and gappedBLAST programs, the default parameters of the respective programs (e.g.,XBLAST and NBLAST) can be used.

[0084] Full-length pre-processed forms, as well as mature processedforms, of proteins that comprise one of the peptides of the presentinvention can readily be identified as having complete sequence identityto one of the transporter peptides of the present invention as well asbeing encoded by the same genetic locus as the transporter peptideprovided herein. As indicated by the data presented in FIG. 3, the mapposition was determined to be on chromosome 10 by ePCR, and confirmedwith radiation hybrid mapping.

[0085] Allelic variants of a transporter peptide can readily beidentified as being a human protein having a high degree (significant)of sequence homology/identity to at least a portion of the transporterpeptide as well as being encoded by the same genetic locus as thetransporter peptide provided herein. Genetic locus can readily bedetermined based on the genomic information provided in FIG. 3, such asthe genomic sequence mapped to the reference human. As indicated by thedata presented in FIG. 3, the map position was determined to be onchromosome 10 by ePCR, and confirmed with radiation hybrid mapping. Asused herein, two proteins (or a region of the proteins) have significanthomology when the amino acid sequences are typically at least about70-80%, 80-90%, and more typically at least about 90-95% or morehomologous. A significantly homologous amino acid sequence, according tothe present invention, will be encoded by a nucleic acid sequence thatwill hybridize to a transporter peptide encoding nucleic acid moleculeunder stringent conditions as more fully described below.

[0086]FIG. 3 provides information on SNPs that have been found in thegene encoding the transporter protein of the present invention. SNPswere identified at 47 different nucleotide positions. Changes in theamino acid sequence caused by these SNPs is indicated in FIG. 3 and canreadily be determined using the universal genetic code and the proteinsequence provided in FIG. 2 as a reference. Some of these SNPs that arelocated outside the ORF and in introns may affect gene transcription.

[0087] Paralogs of a transporter peptide can readily be identified ashaving some degree of significant sequence homology/identity to at leasta portion of the transporter peptide, as being encoded by a gene fromhumans, and as having similar activity or function. Two proteins willtypically be considered paralogs when the amino acid sequences aretypically at least about 60% or greater, and more typically at leastabout 70% or greater homology through a given region or domain. Suchparalogs will be encoded by a nucleic acid sequence that will hybridizeto a transporter peptide encoding nucleic acid molecule under moderateto stringent conditions as more fully described below.

[0088] Orthologs of a transporter peptide can readily be identified ashaving some degree of significant sequence homology/identity to at leasta portion of the transporter peptide as well as being encoded by a genefrom another organism. Preferred orthologs will be isolated frommammals, preferably primates, for the development of human therapeutictargets and agents. Such orthologs will be encoded by a nucleic acidsequence that will hybridize to a transporter peptide encoding nucleicacid molecule under moderate to stringent conditions, as more fullydescribed below, depending on the degree of relatedness of the twoorganisms yielding the proteins.

[0089] Non-naturally occurring variants of the transporter peptides ofthe present invention can readily be generated using recombinanttechniques. Such variants include, but are not limited to deletions,additions and substitutions in the amino acid sequence of thetransporter peptide. For example, one class of substitutions areconserved amino acid substitution. Such substitutions are those thatsubstitute a given amino acid in a transporter peptide by another aminoacid of like characteristics. Typically seen as conservativesubstitutions are the replacements, one for another, among the aliphaticamino acids Ala, Val, Leu, and Ile; interchange of the hydroxyl residuesSer and Thr; exchange of the acidic residues Asp and Glu; substitutionbetween the amide residues Asn and Gln; exchange of the basic residuesLys and Arg; and replacements among the aromatic residues Phe and Tyr.Guidance concerning which amino acid changes are likely to bephenotypically silent are found in Bowie et al., Science 247:1306-1310(1990).

[0090] Variant transporter peptides can be fully functional or can lackfunction in one or more activities, e.g. ability to bind ligand, abilityto transport ligand, ability to mediate signaling, etc. Fully functionalvariants typically contain only conservative variation or variation innon-critical residues or in non-critical regions. FIG. 2 provides theresult of protein analysis and can be used to identify criticaldomains/regions. Functional variants can also contain substitution ofsimilar amino acids that result in no change or an insignificant changein function. Alternatively, such substitutions may positively ornegatively affect function to some degree.

[0091] Non-functional variants typically contain one or morenon-conservative amino acid substitutions, deletions, insertions,inversions, or truncation or a substitution, insertion, inversion, ordeletion in a critical residue or critical region.

[0092] Amino acids that are essential for function can be identified bymethods known in the art, such as site-directed mutagenesis oralanine-scanning mutagenesis (Cunningham et al., Science 244:1081-1085(1989)), particularly using the results provided in FIG. 2. The latterprocedure introduces single alanine mutations at every residue in themolecule. The resulting mutant molecules are then tested for biologicalactivity such as transporter activity or in assays such as an in vitroproliferative activity. Sites that are critical for bindingpartner/substrate binding can also be determined by structural analysissuch as crystallization, nuclear magnetic resonance or photoaffinitylabeling (Smith et al., J. Mol. Biol. 224:899-904 (1992); de Vos et al.Science 255:306-312 (1992)).

[0093] The present invention further provides fragments of thetransporter peptides, in addition to proteins and peptides that compriseand consist of such fragments, particularly those comprising theresidues identified in FIG. 2. The fragments to which the inventionpertains, however, are not to be construed as encompassing fragmentsthat may be disclosed publicly prior to the present invention.

[0094] As used herein, a fragment comprises at least 8, 10, 12, 14, 16,or more contiguous amino acid residues from a transporter peptide. Suchfragments can be chosen based on the ability to retain one or more ofthe biological activities of the transporter peptide or could be chosenfor the ability to perform a function, e.g. bind a substrate or act asan immunogen. Particularly important fragments are biologically activefragments, peptides that are, for example, about 8 or more amino acidsin length. Such fragments will typically comprise a domain or motif ofthe transporter peptide, e.g., active site, a transmembrane domain or asubstrate-binding domain. Further, possible fragments include, but arenot limited to, domain or motif containing fragments, soluble peptidefragments, and fragments containing immunogenic structures. Predicteddomains and functional sites are readily identifiable by computerprograms well known and readily available to those of skill in the art(e.g., PROSITE analysis). The results of one such analysis are providedin FIG. 2.

[0095] Polypeptides often contain amino acids other than the 20 aminoacids commonly referred to as the 20 naturally occurring amino acids.Further, many amino acids, including the terminal amino acids, may bemodified by natural processes, such as processing and otherpost-translational modifications, or by chemical modification techniqueswell known in the art. Common modifications that occur naturally intransporter peptides are described in basic texts, detailed monographs,and the research literature, and they are well known to those of skillin the art (some of these features are identified in FIG. 2).

[0096] Known modifications include, but are not limited to, acetylation,acylation, ADP-ribosylation, amidation, covalent attachment of flavin,covalent attachment of a heme moiety, covalent attachment of anucleotide or nucleotide derivative, covalent attachment of a lipid orlipid derivative, covalent attachment of phosphotidylinositol,cross-linking, cyclization, disulfide bond formation, demethylation,formation of covalent crosslinks, formation of cystine, formation ofpyroglutamate, formylation, gamma carboxylation, glycosylation, GPIanchor formation, hydroxylation, iodination, methylation,myristoylation, oxidation, proteolytic processing, phosphorylation,prenylation, racemization, selenoylation, sulfation, transfer-RNAmediated addition of amino acids to proteins such as arginylation, andubiquitination.

[0097] Such modifications are well known to those of skill in the artand have been described in great detail in the scientific literature.Several particularly common modifications, glycosylation, lipidattachment, sulfation, gamma-carboxylation of glutamic acid residues,hydroxylation and ADP-ribosylation, for instance, are described in mostbasic texts, such as Proteins—Structure and Molecular Properties, 2ndEd., T. E. Creighton, W. H. Freeman and Company, New York (1993). Manydetailed reviews are available on this subject, such as by Wold, F.,Posttranslational Covalent Modification of Proteins, B. C. Johnson, Ed.,Academic Press, New York 1-12 (1983); Seifter et al. (Meth. Enzymol.182: 626-646 (1990)) and Rattan et al. (Ann. N.Y Acad. Sci. 663:48-62(1992)).

[0098] Accordingly, the transporter peptides of the present inventionalso encompass derivatives or analogs in which a substituted amino acidresidue is not one encoded by the genetic code, in which a substituentgroup is included, in which the mature transporter peptide is fused withanother compound, such as a compound to increase the half-life of thetransporter peptide (for example, polyethylene glycol), or in which theadditional amino acids are fused to the mature transporter peptide, suchas a leader or secretory sequence or a sequence for purification of themature transporter peptide or a pro-protein sequence.

[0099] Protein/Peptide Uses

[0100] The proteins of the present invention can be used in substantialand specific assays related to the functional information provided inthe Figures; to raise antibodies or to elicit another immune response;as a reagent (including the labeled reagent) in assays designed toquantitatively determine levels of the protein (or its binding partneror ligand) in biological fluids; and as markers for tissues in which thecorresponding protein is preferentially expressed (either constitutivelyor at a particular stage of tissue differentiation or development or ina disease state). Where the protein binds or potentially binds toanother protein or ligand (such as, for example, in atransporter-effector protein interaction or transporter-ligandinteraction), the protein can be used to identify the bindingpartner/ligand so as to develop a system to identify inhibitors of thebinding interaction. Any or all of these uses are capable of beingdeveloped into reagent grade or kit format for commercialization ascommercial products.

[0101] Methods for performing the uses listed above are well known tothose skilled in the art. References disclosing such methods include“Molecular Cloning: A Laboratory Manual”, 2d ed., Cold Spring HarborLaboratory Press, Sambrook, J., E. F. Fritsch and T. Maniatis eds.,1989, and “Methods in Enzymology: Guide to Molecular CloningTechniques”, Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.

[0102] The potential uses of the peptides of the present invention arebased primarily on the source of the protein as well as the class/actionof the protein. For example, transporters isolated from humans and theirhuman/mammalian orthologs serve as targets for identifying agents foruse in mammalian therapeutic applications, e.g. a human drug,particularly in modulating a biological or pathological response in acell or tissue that expresses the transporter. Experimental data asprovided in FIG. 1 indicates that the transporter proteins of thepresent invention are expressed in humans in the liver, adrenal gland,normal and tumorous nervous tissue, adult amygdala, brain meningiomatissue, denis-drash, adult and fetal brain, placenta, testis and kidney.Specifically, a virtual northern blot shows expression in the liver,adrenal gland, normal and tumorous nervous tissues, adult amygdala,brain meningioma tissue, and denis-drash. In addition, PCR-based tissuescreening panels indicate expression in the adult and fetal brain,placenta, testis, and kidney. A large percentage of pharmaceuticalagents are being developed that modulate the activity of transporterproteins, particularly members of the calcium channel subfamily (seeBackground of the Invention). The structural and functional informationprovided in the Background and Figures provide specific and substantialuses for the molecules of the present invention, particularly incombination with the expression information provided in FIG. 1.Experimental data as provided in FIG. 1 indicates expression in humansin the liver, adrenal gland, normal and tumorous nervous tissue, adultamygdala, brain meningioma tissue, denis-drash, adult and fetal brain,placenta, testis and kidney. Such uses can readily be determined usingthe information provided herein, that known in the art and routineexperimentation.

[0103] The proteins of the present invention (including variants andfragments that may have been disclosed prior to the present invention)are useful for biological assays related to transporters that arerelated to members of the calcium channel subfamily. Such assays involveany of the known transporter functions or activities or propertiesuseful for diagnosis and treatment of transporter-related conditionsthat are specific for the subfamily of transporters that the one of thepresent invention belongs to, particularly in cells and tissues thatexpress the transporter. Experimental data as provided in FIG. 1indicates that the transporter proteins of the present invention areexpressed in humans in the liver, adrenal gland, normal and tumorousnervous tissue, adult amygdala, brain meningioma tissue, denis-drash,adult and fetal brain, placenta, testis and kidney. Specifically, avirtual northern blot shows expression in the liver, adrenal gland,normal and tumorous nervous tissues, adult amygdala, brain meningiomatissue, and denis-drash. In addition, PCR-based tissue screening panelsindicate expression in the adult and fetal brain, placenta, testis, andkidney. The proteins of the present invention are also useful in drugscreening assays, in cell-based or cell-free systems ((Hodgson,Bio/technology, Sep. 10, 1992 (9);973-80). Cell-based systems can benative, i.e., cells that normally express the transporter, as a biopsyor expanded in cell culture. Experimental data as provided in FIG. 1indicates expression in humans in the liver, adrenal gland, normal andtumorous nervous tissue, adult amygdala, brain meningioma tissue,denis-drash, adult and fetal brain, placenta, testis and kidney. In analternate embodiment, cell-based assays involve recombinant host cellsexpressing the transporter protein.

[0104] The polypeptides can be used to identify compounds that modulatetransporter activity of the protein in its natural state or an alteredform that causes a specific disease or pathology associated with thetransporter. Both the transporters of the present invention andappropriate variants and fragments can be used in high-throughputscreens to assay candidate compounds for the ability to bind to thetransporter. These compounds can be further screened against afunctional transporter to determine the effect of the compound on thetransporter activity. Further, these compounds can be tested in animalor invertebrate systems to determine activity/effectiveness. Compoundscan be identified that activate (agonist) or inactivate (antagonist) thetransporter to a desired degree.

[0105] Further, the proteins of the present invention can be used toscreen a compound for the ability to stimulate or inhibit interactionbetween the transporter protein and a molecule that normally interactswith the transporter protein, e.g. a substrate or a component of thesignal pathway that the transporter protein normally interacts (forexample, another transporter). Such assays typically include the stepsof combining the transporter protein with a candidate compound underconditions that allow the transporter protein, or fragment, to interactwith the target molecule, and to detect the formation of a complexbetween the protein and the target or to detect the biochemicalconsequence of the interaction with the transporter protein and thetarget, such as any of the associated effects of signal transductionsuch as changes in membrane potential, protein phosphorylation, cAMPturnover, and adenylate cyclase activation, etc.

[0106] Candidate compounds include, for example, 1) peptides such assoluble peptides, including Ig-tailed fusion peptides and members ofrandom peptide libraries (see, e.g., Lam et al., Nature 354:82-84(1991); Houghten et al., Nature 354:84-86 (1991)) and combinatorialchemistry-derived molecular libraries made of D- and/or L-configurationamino acids; 2) phosphopeptides (e.g., members of random and partiallydegenerate, directed phosphopeptide libraries, see, e.g., Songyang etal., Cell 72:767-778 (1993)); 3) antibodies (e.g., polyclonal,monoclonal, humanized, anti-idiotypic, chimeric, and single chainantibodies as well as Fab, F(ab′)₂, Fab expression library fragments,and epitope-binding fragments of antibodies); and 4) small organic andinorganic molecules (e.g., molecules obtained from combinatorial andnatural product libraries).

[0107] One candidate compound is a soluble fragment of the receptor thatcompetes for ligand binding. Other candidate compounds include mutanttransporters or appropriate fragments containing mutations that affecttransporter function and thus compete for ligand. Accordingly, afragment that competes for ligand, for example with a higher affinity,or a fragment that binds ligand but does not allow release, isencompassed by the invention.

[0108] The invention further includes other end point assays to identifycompounds that modulate (stimulate or inhibit) transporter activity. Theassays typically involve an assay of events in the signal transductionpathway that indicate transporter activity. Thus, the transport of aligand, change in cell membrane potential, activation of a protein, achange in the expression of genes that are up- or down-regulated inresponse to the transporter protein dependent signal cascade can beassayed.

[0109] Any of the biological or biochemical functions mediated by thetransporter can be used as an endpoint assay. These include all of thebiochemical or biochemical/biological events described herein, in thereferences cited herein, incorporated by reference for these endpointassay targets, and other functions known to those of ordinary skill inthe art or that can be readily identified using the information providedin the Figures, particularly FIG. 2. Specifically, a biological functionof a cell or tissues that expresses the transporter can be assayed.Experimental data as provided in FIG. 1 indicates that the transporterproteins of the present invention are expressed in humans in the liver,adrenal gland, normal and tumorous nervous tissue, adult amygdala, brainmeningioma tissue, denis-drash, adult and fetal brain, placenta, testisand kidney. Specifically, a virtual northern blot shows expression inthe liver, adrenal gland, normal and tumorous nervous tissues, adultamygdala, brain meningioma tissue, and denis-drash. In addition,PCR-based tissue screening panels indicate expression in the adult andfetal brain, placenta, testis, and kidney.

[0110] Binding and/or activating compounds can also be screened by usingchimeric transporter proteins in which the amino terminal extracellulardomain, or parts thereof, the entire transmembrane domain or subregions,such as any of the seven transmembrane segments or any of theintracellular or extracellular loops and the carboxy terminalintracellular domain, or parts thereof, can be replaced by heterologousdomains or subregions. For example, a ligand-binding region can be usedthat interacts with a different ligand then that which is recognized bythe native transporter. Accordingly, a different set of signaltransduction components is available as an end-point assay foractivation. This allows for assays to be performed in other than thespecific host cell from which the transporter is derived.

[0111] The proteins of the present invention are also useful incompetition binding assays in methods designed to discover compoundsthat interact with the transporter (e.g. binding partners and/orligands). Thus, a compound is exposed to a transporter polypeptide underconditions that allow the compound to bind or to otherwise interact withthe polypeptide. Soluble transporter polypeptide is also added to themixture. If the test compound interacts with the soluble transporterpolypeptide, it decreases the amount of complex formed or activity fromthe transporter target. This type of assay is particularly useful incases in which compounds are sought that interact with specific regionsof the transporter. Thus, the soluble polypeptide that competes with thetarget transporter region is designed to contain peptide sequencescorresponding to the region of interest.

[0112] To perform cell free drug screening assays, it is sometimesdesirable to immobilize either the transporter protein, or fragment, orits target molecule to facilitate separation of complexes fromuncomplexed forms of one or both of the proteins, as well as toaccommodate automation of the assay.

[0113] Techniques for immobilizing proteins on matrices can be used inthe drug screening assays. In one embodiment, a fusion protein can beprovided which adds a domain that allows the protein to be bound to amatrix. For example, glutathione-S-transferase fusion proteins can beadsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis,Mo.) or glutathione derivatized microtitre plates, which are thencombined with the cell lysates (e.g., ³⁵S-labeled) and the candidatecompound, and the mixture incubated under conditions conducive tocomplex formation (e.g., at physiological conditions for salt and pH).Following incubation, the beads are washed to remove any unbound label,and the matrix immobilized and radiolabel determined directly, or in thesupernatant after the complexes are dissociated. Alternatively, thecomplexes can be dissociated from the matrix, separated by SDS-PAGE, andthe level of transporter-binding protein found in the bead fractionquantitated from the gel using standard electrophoretic techniques. Forexample, either the polypeptide or its target molecule can beimmobilized utilizing conjugation of biotin and streptavidin usingtechniques well known in the art. Alternatively, antibodies reactivewith the protein but which do not interfere with binding of the proteinto its target molecule can be derivatized to the wells of the plate, andthe protein trapped in the wells by antibody conjugation. Preparationsof a transporter-binding protein and a candidate compound are incubatedin the transporter protein-presenting wells and the amount of complextrapped in the well can be quantitated. Methods for detecting suchcomplexes, in addition to those described above for the GST-immobilizedcomplexes, include immunodetection of complexes using antibodiesreactive with the transporter protein target molecule, or which arereactive with transporter protein and compete with the target molecule,as well as enzyme-linked assays which rely on detecting an enzymaticactivity associated with the target molecule.

[0114] Agents that modulate one of the transporters of the presentinvention can be identified using one or more of the above assays, aloneor in combination. It is generally preferable to use a cell-based orcell free system first and then confirm activity in an animal or othermodel system. Such model systems are well known in the art and canreadily be employed in this context.

[0115] Modulators of transporter protein activity identified accordingto these drug screening assays can be used to treat a subject with adisorder mediated by the transporter pathway, by treating cells ortissues that express the transporter. Experimental data as provided inFIG. 1 indicates expression in humans in the liver, adrenal gland,normal and tumorous nervous tissue, adult amygdala, brain meningiomatissue, denis-drash, adult and fetal brain, placenta, testis and kidney.These methods of treatment include the steps of administering amodulator of transporter activity in a pharmaceutical composition to asubject in need of such treatment, the modulator being identified asdescribed herein.

[0116] In yet another aspect of the invention, the transporter proteinscan be used as “bait proteins” in a two-hybrid assay or three-hybridassay (see, e.g., U.S. Pat. No. 5,283,317; Zervos et al. (1993) Cell72:223-232; Madura et al. (1993) J. Biol. Chem. 268:12046-12054; Bartelet al. (1993) Biotechniques 14:920-924; Iwabuchi et al. (1993) Oncogene8:1693-1696; and Brent WO94/10300), to identify other proteins, whichbind to or interact with the transporter and are involved in transporteractivity. Such transporter-binding proteins are also likely to beinvolved in the propagation of signals by the transporter proteins ortransporter targets as, for example, downstream elements of atransporter-mediated signaling pathway. Alternatively, suchtransporter-binding proteins are likely to be transporter inhibitors.

[0117] The two-hybrid system is based on the modular nature of mosttranscription factors, which consist of separable DNA-binding andactivation domains. Briefly, the assay utilizes two different DNAconstructs. In one construct, the gene that codes for a transporterprotein is fused to a gene encoding the DNA binding domain of a knowntranscription factor (e.g., GAL-4). In the other construct, a DNAsequence, from a library of DNA sequences, that encodes an unidentifiedprotein (“prey” or “sample”) is fused to a gene that codes for theactivation domain of the known transcription factor. If the “bait” andthe “prey” proteins are able to interact, in vivo, forming atransporter-dependent complex, the DNA-binding and activation domains ofthe transcription factor are brought into close proximity. Thisproximity allows transcription of a reporter gene (e.g., LacZ) which isoperably linked to a transcriptional regulatory site responsive to thetranscription factor. Expression of the reporter gene can be detectedand cell colonies containing the functional transcription factor can beisolated and used to obtain the cloned gene which encodes the proteinwhich interacts with the transporter protein.

[0118] This invention further pertains to novel agents identified by theabove-described screening assays. Accordingly, it is within the scope ofthis invention to further use an agent identified as described herein inan appropriate animal model. For example, an agent identified asdescribed herein (e.g., a transporter-modulating agent, an antisensetransporter nucleic acid molecule, a transporter-specific antibody, or atransporter-binding partner) can be used in an animal or other model todetermine the efficacy, toxicity, or side effects of treatment with suchan agent. Alternatively, an agent identified as described herein can beused in an animal or other model to determine the mechanism of action ofsuch an agent. Furthermore, this invention pertains to uses of novelagents identified by the above-described screening assays for treatmentsas described herein.

[0119] The transporter proteins of the present invention are also usefulto provide a target for diagnosing a disease or predisposition todisease mediated by the peptide. Accordingly, the invention providesmethods for detecting the presence, or levels of, the protein (orencoding mRNA) in a cell, tissue, or organism. Experimental data asprovided in FIG. 1 indicates expression in humans in the liver, adrenalgland, normal and tumorous nervous tissue, adult amygdala, brainmeningioma tissue, denis-drash, adult and fetal brain, placenta, testisand kidney. The method involves contacting a biological sample with acompound capable of interacting with the transporter protein such thatthe interaction can be detected. Such an assay can be provided in asingle detection format or a multi-detection format such as an antibodychip array.

[0120] One agent for detecting a protein in a sample is an antibodycapable of selectively binding to protein. A biological sample includestissues, cells and biological fluids isolated from a subject, as well astissues, cells and fluids present within a subject.

[0121] The peptides of the present invention also provide targets fordiagnosing active protein activity, disease, or predisposition todisease, in a patient having a variant peptide, particularly activitiesand conditions that are known for other members of the family ofproteins to which the present one belongs. Thus, the peptide can beisolated from a biological sample and assayed for the presence of agenetic mutation that results in aberrant peptide. This includes aminoacid substitution, deletion, insertion, rearrangement, (as the result ofaberrant splicing events), and inappropriate post-translationalmodification. Analytic methods include altered electrophoretic mobility,altered tryptic peptide digest, altered transporter activity incell-based or cell-free assay, alteration in ligand or antibody-bindingpattern, altered isoelectric point, direct amino acid sequencing, andany other of the known assay techniques useful for detecting mutationsin a protein. Such an assay can be provided in a single detection formator a multi-detection format such as an antibody chip array.

[0122] In vitro techniques for detection of peptide include enzymelinked immunosorbent assays (ELISAs), Western blots,immunoprecipitations and immunofluorescence using a detection reagent,such as an antibody or protein binding agent. Alternatively, the peptidecan be detected in vivo in a subject by introducing into the subject alabeled anti-peptide antibody or other types of detection agent. Forexample, the antibody can be labeled with a radioactive marker whosepresence and location in a subject can be detected by standard imagingtechniques. Particularly useful are methods that detect the allelicvariant of a peptide expressed in a subject and methods which detectfragments of a peptide in a sample.

[0123] The peptides are also useful in pharmacogenomic analysis.Pharmacogenomics deal with clinically significant hereditary variationsin the response to drugs due to altered drug disposition and abnormalaction in affected persons. See, e.g., Eichelbaum, M. (Clin. Exp.Pharmacol. Physiol. 23(10-11):983-985 (1996)), and Linder, M. W. (Clin.Chem. 43(2):254-266 (1997)). The clinical outcomes of these variationsresult in severe toxicity of therapeutic drugs in certain individuals ortherapeutic failure of drugs in certain individuals as a result ofindividual variation in metabolism. Thus, the genotype of the individualcan determine the way a therapeutic compound acts on the body or the waythe body metabolizes the compound. Further, the activity of drugmetabolizing enzymes effects both the intensity and duration of drugaction. Thus, the pharmacogenomics of the individual permit theselection of effective compounds and effective dosages of such compoundsfor prophylactic or therapeutic treatment based on the individual'sgenotype. The discovery of genetic polymorphisms in some drugmetabolizing enzymes has explained why some patients do not obtain theexpected drug effects, show an exaggerated drug effect, or experienceserious toxicity from standard drug dosages. Polymorphisms can beexpressed in the phenotype of the extensive metabolizer and thephenotype of the poor metabolizer. Accordingly, genetic polymorphism maylead to allelic protein variants of the transporter protein in which oneor more of the transporter functions in one population is different fromthose in another population. The peptides thus allow a target toascertain a genetic predisposition that can affect treatment modality.Thus, in a ligand-based treatment, polymorphism may give rise to aminoterminal extracellular domains and/or other ligand-binding regions thatare more or less active in ligand binding, and transporter activation.Accordingly, ligand dosage would necessarily be modified to maximize thetherapeutic effect within a given population containing a polymorphism.As an alternative to genotyping, specific polymorphic peptides could beidentified.

[0124] The peptides are also useful for treating a disordercharacterized by an absence of, inappropriate, or unwanted expression ofthe protein. Experimental data as provided in FIG. 1 indicatesexpression in humans in the liver, adrenal gland, normal and tumorousnervous tissue, adult amygdala, brain meningioma tissue, denis-drash,adult and fetal brain, placenta, testis and kidney. Accordingly, methodsfor treatment include the use of the transporter protein or fragments.

[0125] Antibodies

[0126] The invention also provides antibodies that selectively bind toone of the peptides of the present invention, a protein comprising sucha peptide, as well as variants and fragments thereof. As used herein, anantibody selectively binds a target peptide when it binds the targetpeptide and does not significantly bind to unrelated proteins. Anantibody is still considered to selectively bind a peptide even if italso binds to other proteins that are not substantially homologous withthe target peptide so long as such proteins share homology with afragment or domain of the peptide target of the antibody. In this case,it would be understood that antibody binding to the peptide is stillselective despite some degree of cross-reactivity.

[0127] As used herein, an antibody is defined in terms consistent withthat recognized within the art: they are multi-subunit proteins producedby a mammalian organism in response to an antigen challenge. Theantibodies of the present invention include polyclonal antibodies andmonoclonal antibodies, as well as fragments of such antibodies,including, but not limited to, Fab or F(ab′)₂, and Fv fragments.

[0128] Many methods are known for generating and/or identifyingantibodies to a given target peptide. Several such methods are describedby Harlow, Antibodies, Cold Spring Harbor Press, (1989).

[0129] In general, to generate antibodies, an isolated peptide is usedas an immunogen and is administered to a mammalian organism, such as arat, rabbit or mouse. The full-length protein, an antigenic peptidefragment or a fusion protein can be used. Particularly importantfragments are those covering functional domains, such as the domainsidentified in FIG. 2, and domain of sequence homology or divergenceamongst the family, such as those that can readily be identified usingprotein alignment methods and as presented in the Figures.

[0130] Antibodies are preferably prepared from regions or discretefragments of the transporter proteins. Antibodies can be prepared fromany region of the peptide as described herein. However, preferredregions will include those involved in function/activity and/ortransporter/binding partner interaction. FIG. 2 can be used to identifyparticularly important regions while sequence alignment can be used toidentify conserved and unique sequence fragments.

[0131] An antigenic fragment will typically comprise at least 8contiguous amino acid residues. The antigenic peptide can comprise,however, at least 10, 12, 14, 16 or more amino acid residues. Suchfragments can be selected on a physical property, such as fragmentscorrespond to regions that are located on the surface of the protein,e.g., hydrophilic regions or can be selected based on sequenceuniqueness (see FIG. 2).

[0132] Detection on an antibody of the present invention can befacilitated by coupling (i.e., physically linking) the antibody to adetectable substance. Examples of detectable substances include variousenzymes, prosthetic groups, fluorescent materials, luminescentmaterials, bioluminescent materials, and radioactive materials. Examplesof suitable enzymes include horseradish peroxidase, alkalinephosphatase, β-galactosidase, or acetylcholinesterase; examples ofsuitable prosthetic group complexes include streptavidin/biotin andavidin/biotin; examples of suitable fluorescent materials includeumbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine,dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; anexample of a luminescent material includes luminol; examples ofbioluminescent materials include luciferase, luciferin, and aequorin,and examples of suitable radioactive material include ¹²⁵I, ¹³¹I, ³⁵S or³H.

[0133] Antibody Uses

[0134] The antibodies can be used to isolate one of the proteins of thepresent invention by standard techniques, such as affinitychromatography or immunoprecipitation. The antibodies can facilitate thepurification of the natural protein from cells and recombinantlyproduced protein expressed in host cells. In addition, such antibodiesare useful to detect the presence of one of the proteins of the presentinvention in cells or tissues to determine the pattern of expression ofthe protein among various tissues in an organism and over the course ofnormal development. Experimental data as provided in FIG. 1 indicatesthat the transporter proteins of the present invention are expressed inhumans in the liver, adrenal gland, normal and tumorous nervous tissue,adult amygdala, brain meningioma tissue, denis-drash, adult and fetalbrain, placenta, testis and kidney. Specifically, a virtual northernblot shows expression in the liver, adrenal gland, normal and tumorousnervous tissues, adult amygdala, brain meningioma tissue, anddenis-drash. In addition, PCR-based tissue screening panels indicateexpression in the adult and fetal brain, placenta, testis, and kidney.Further, such antibodies can be used to detect protein in situ, invitro, or in a cell lysate or supernatant in order to evaluate theabundance and pattern of expression. Also, such antibodies can be usedto assess abnormal tissue distribution or abnormal expression duringdevelopment or progression of a biological condition. Antibody detectionof circulating fragments of the full length protein can be used toidentify turnover.

[0135] Further, the antibodies can be used to assess expression indisease states such as in active stages of the disease or in anindividual with a predisposition toward disease related to the protein'sfunction. When a disorder is caused by an inappropriate tissuedistribution, developmental expression, level of expression of theprotein, or expressed/processed form, the antibody can be preparedagainst the normal protein. Experimental data as provided in FIG. 1indicates expression in humans in the liver, adrenal gland, normal andtumorous nervous tissue, adult amygdala, brain meningioma tissue,denis-drash, adult and fetal brain, placenta, testis and kidney. If adisorder is characterized by a specific mutation in the protein,antibodies specific for this mutant protein can be used to assay for thepresence of the specific mutant protein.

[0136] The antibodies can also be used to assess normal and aberrantsubcellular localization of cells in the various tissues in an organism.Experimental data as provided in FIG. 1 indicates expression in humansin the liver, adrenal gland, normal and tumorous nervous tissue, adultamygdala, brain meningioma tissue, denis-drash, adult and fetal brain,placenta, testis and kidney. The diagnostic uses can be applied, notonly in genetic testing, but also in monitoring a treatment modality.Accordingly, where treatment is ultimately aimed at correctingexpression level or the presence of aberrant sequence and aberranttissue distribution or developmental expression, antibodies directedagainst the protein or relevant fragments can be used to monitortherapeutic efficacy.

[0137] Additionally, antibodies are useful in pharmacogenomic analysis.Thus, antibodies prepared against polymorphic proteins can be used toidentify individuals that require modified treatment modalities. Theantibodies are also useful as diagnostic tools as an immunologicalmarker for aberrant protein analyzed by electrophoretic mobility,isoelectric point, tryptic peptide digest, and other physical assaysknown to those in the art.

[0138] The antibodies are also useful for tissue typing. Experimentaldata as provided in FIG. 1 indicates expression in humans in the liver,adrenal gland, normal and tumorous nervous tissue, adult amygdala, brainmeningioma tissue, denis-drash, adult and fetal brain, placenta, testisand kidney. Thus, where a specific protein has been correlated withexpression in a specific tissue, antibodies that are specific for thisprotein can be used to identify a tissue type.

[0139] The antibodies are also useful for inhibiting protein function,for example, blocking the binding of the transporter peptide to abinding partner such as a ligand or protein binding partner. These usescan also be applied in a therapeutic context in which treatment involvesinhibiting the protein's function. An antibody can be used, for example,to block binding, thus modulating (agonizing or antagonizing) thepeptides activity. Antibodies can be prepared against specific fragmentscontaining sites required for function or against intact protein that isassociated with a cell or cell membrane. See FIG. 2 for structuralinformation relating to the proteins of the present invention.

[0140] The invention also encompasses kits for using antibodies todetect the presence of a protein in a biological sample. The kit cancomprise antibodies such as a labeled or labelable antibody and acompound or agent for detecting protein in a biological sample; meansfor determining the amount of protein in the sample; means for comparingthe amount of protein in the sample with a standard; and instructionsfor use. Such a kit can be supplied to detect a single protein orepitope or can be configured to detect one of a multitude of epitopes,such as in an antibody detection array. Arrays are described in detailbelow for nucleic acid arrays and similar methods have been developedfor antibody arrays.

[0141] Nucleic Acid Molecules

[0142] The present invention further provides isolated nucleic acidmolecules that encode a transporter peptide or protein of the presentinvention (cDNA, transcript and genomic sequence). Such nucleic acidmolecules will consist of, consist essentially of, or comprise anucleotide sequence that encodes one of the transporter peptides of thepresent invention, an allelic variant thereof, or an ortholog or paralogthereof.

[0143] As used herein, an “isolated” nucleic acid molecule is one thatis separated from other nucleic acid present in the natural source ofthe nucleic acid. Preferably, an “isolated” nucleic acid is free ofsequences that naturally flank the nucleic acid (i.e., sequences locatedat the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of theorganism from which the nucleic acid is derived. However, there can besome flanking nucleotide sequences, for example up to about 5 KB, 4 KB,3 KB, 2 KB, or 1 KB or less, particularly contiguous peptide encodingsequences and peptide encoding sequences within the same gene butseparated by introns in the genomic sequence. The important point isthat the nucleic acid is isolated from remote and unimportant flankingsequences such that it can be subjected to the specific manipulationsdescribed herein such as recombinant expression, preparation of probesand primers, and other uses specific to the nucleic acid sequences.

[0144] Moreover, an “isolated” nucleic acid molecule, such as atranscript/cDNA molecule, can be substantially free of other cellularmaterial, or culture medium when produced by recombinant techniques, orchemical precursors or other chemicals when chemically synthesized.However, the nucleic acid molecule can be fused to other coding orregulatory sequences and still be considered isolated.

[0145] For example, recombinant DNA molecules contained in a vector areconsidered isolated. Further examples of isolated DNA molecules includerecombinant DNA molecules maintained in heterologous host cells orpurified (partially or substantially) DNA molecules in solution.Isolated RNA molecules include in vivo or in vitro RNA transcripts ofthe isolated DNA molecules of the present invention. Isolated nucleicacid molecules according to the present invention further include suchmolecules produced synthetically.

[0146] Accordingly, the present invention provides nucleic acidmolecules that consist of the nucleotide sequence shown in FIGS. 1 or 3(SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), orany nucleic acid molecule that encodes the protein provided in FIG. 2,SEQ ID NO:2. A nucleic acid molecule consists of a nucleotide sequencewhen the nucleotide sequence is the complete nucleotide sequence of thenucleic acid molecule.

[0147] The present invention further provides nucleic acid moleculesthat consist essentially of the nucleotide sequence shown in FIGS. 1 or3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence),or any nucleic acid molecule that encodes the protein provided in FIG.2, SEQ ID NO:2. A nucleic acid molecule consists essentially of anucleotide sequence when such a nucleotide sequence is present with onlya few additional nucleic acid residues in the final nucleic acidmolecule.

[0148] The present invention further provides nucleic acid moleculesthat comprise the nucleotide sequences shown in FIGS. 1 or 3 (SEQ IDNO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or anynucleic acid molecule that encodes the protein provided in FIG. 2, SEQID NO:2. A nucleic acid molecule comprises a nucleotide sequence whenthe nucleotide sequence is at least part of the final nucleotidesequence of the nucleic acid molecule. In such a fashion, the nucleicacid molecule can be only the nucleotide sequence or have additionalnucleic acid residues, such as nucleic acid residues that are naturallyassociated with it or heterologous nucleotide sequences. Such a nucleicacid molecule can have a few additional nucleotides or can compriseseveral hundred or more additional nucleotides. A brief description ofhow various types of these nucleic acid molecules can be readilymade/isolated is provided below.

[0149] In FIGS. 1 and 3, both coding and non-coding sequences areprovided. Because of the source of the present invention, humans genomicsequence (FIG. 3) and cDNA/transcript sequences (FIG. 1), the nucleicacid molecules in the Figures will contain genomic intronic sequences,5′ and 3′ non-coding sequences, gene regulatory regions and non-codingintergenic sequences. In general such sequence features are either notedin FIGS. 1 and 3 or can readily be identified using computational toolsknown in the art. As discussed below, some of the non-coding regions,particularly gene regulatory elements such as promoters, are useful fora variety of purposes, e.g. control of heterologous gene expression,target for identifying gene activity modulating compounds, and areparticularly claimed as fragments of the genomic sequence providedherein.

[0150] The isolated nucleic acid molecules can encode the mature proteinplus additional amino or carboxyl-terminal amino acids, or amino acidsinterior to the mature peptide (when the mature form has more than onepeptide chain, for instance). Such sequences may play a role inprocessing of a protein from precursor to a mature form, facilitateprotein trafficking, prolong or shorten protein half-life or facilitatemanipulation of a protein for assay or production, among other things.As generally is the case in situ, the additional amino acids may beprocessed away from the mature protein by cellular enzymes.

[0151] As mentioned above, the isolated nucleic acid molecules include,but are not limited to, the sequence encoding the transporter peptidealone, the sequence encoding the mature peptide and additional codingsequences, such as a leader or secretory sequence (e.g., a pre-pro orpro-protein sequence), the sequence encoding the mature peptide, with orwithout the additional coding sequences, plus additional non-codingsequences, for example introns and non-coding 5′ and 3′ sequences suchas transcribed but non-translated sequences that play a role intranscription, mRNA processing (including splicing and polyadenylationsignals), ribosome binding and stability of mRNA. In addition, thenucleic acid molecule may be fused to a marker sequence encoding, forexample, a peptide that facilitates purification.

[0152] Isolated nucleic acid molecules can be in the form of RNA, suchas mRNA, or in the form DNA, including cDNA and genomic DNA obtained bycloning or produced by chemical synthetic techniques or by a combinationthereof. The nucleic acid, especially DNA, can be double-stranded orsingle-stranded. Single-stranded nucleic acid can be the coding strand(sense strand) or the non-coding strand (anti-sense strand).

[0153] The invention further provides nucleic acid molecules that encodefragments of the peptides of the present invention as well as nucleicacid molecules that encode obvious variants of the transporter proteinsof the present invention that are described above. Such nucleic acidmolecules may be naturally occurring, such as allelic variants (samelocus), paralogs (different locus), and orthologs (different organism),or may be constructed by recombinant DNA methods or by chemicalsynthesis. Such non-naturally occurring variants may be made bymutagenesis techniques, including those applied to nucleic acidmolecules, cells, or organisms. Accordingly, as discussed above, thevariants can contain nucleotide substitutions, deletions, inversions andinsertions. Variation can occur in either or both the coding andnon-coding regions. The variations can produce both conservative andnon-conservative amino acid substitutions.

[0154] The present invention further provides non-coding fragments ofthe nucleic acid molecules provided in FIGS. 1 and 3. Preferrednon-coding fragments include, but are not limited to, promotersequences, enhancer sequences, gene modulating sequences and genetermination sequences. Such fragments are useful in controllingheterologous gene expression and in developing screens to identifygene-modulating agents. A promoter can readily be identified as being 5′to the ATG start site in the genomic sequence provided in FIG. 3.

[0155] A fragment comprises a contiguous nucleotide sequence greaterthan 12 or more nucleotides. Further, a fragment could at least 30, 40,50, 100, 250 or 500 nucleotides in length. The length of the fragmentwill be based on its intended use. For example, the fragment can encodeepitope bearing regions of the peptide, or can be useful as DNA probesand primers. Such fragments can be isolated using the known nucleotidesequence to synthesize an oligonucleotide probe. A labeled probe canthen be used to screen a cDNA library, genomic DNA library, or mRNA toisolate nucleic acid corresponding to the coding region. Further,primers can be used in PCR reactions to clone specific regions of gene.

[0156] A probe/primer typically comprises substantially a purifiedoligonucleotide or oligonucleotide pair. The oligonucleotide typicallycomprises a region of nucleotide sequence that hybridizes understringent conditions to at least about 12, 20, 25, 40, 50 or moreconsecutive nucleotides.

[0157] Orthologs, homologs, and allelic variants can be identified usingmethods well known in the art. As described in the Peptide Section,these variants comprise a nucleotide sequence encoding a peptide that istypically 60-70%, 70-80%, 80-90%, and more typically at least about90-95% or more homologous to the nucleotide sequence shown in the Figuresheets or a fragment of this sequence. Such nucleic acid molecules canreadily be identified as being able to hybridize under moderate tostringent conditions, to the nucleotide sequence shown in the Figuresheets or a fragment of the sequence. Allelic variants can readily bedetermined by genetic locus of the encoding gene. As indicated by thedata presented in FIG. 3, the map position was determined to be onchromosome 10 by ePCR, and confirmed with radiation hybrid mapping.

[0158]FIG. 3 provides information on SNPs that have been found in thegene encoding the transporter protein of the present invention. SNPswere identified at 47 different nucleotide positions. Changes in theamino acid sequence caused by these SNPs is indicated in FIG. 3 and canreadily be determined using the universal genetic code and the proteinsequence provided in FIG. 2 as a reference. Some of these SNPs that arelocated outside the ORF and in introns may affect gene transcription.

[0159] As used herein, the term “hybridizes under stringent conditions”is intended to describe conditions for hybridization and washing underwhich nucleotide sequences encoding a peptide at least 60-70% homologousto each other typically remain hybridized to each other. The conditionscan be such that sequences at least about 60%, at least about 70%, or atleast about 80% or more homologous to each other typically remainhybridized to each other. Such stringent conditions are known to thoseskilled in the art and can be found in Current Protocols in MolecularBiology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example ofstringent hybridization conditions are hybridization in 6×sodiumchloride/sodium citrate (SSC) at about 45 C., followed by one or morewashes in 0.2×SSC, 0.1% SDS at 50-65 C. Examples of moderate to lowstringency hybridization conditions are well known in the art.

[0160] Nucleic Acid Molecule Uses

[0161] The nucleic acid molecules of the present invention are usefulfor probes, primers, chemical intermediates, and in biological assays.The nucleic acid molecules are useful as a hybridization probe formessenger RNA, transcript/cDNA and genomic DNA to isolate full-lengthcDNA and genomic clones encoding the peptide described in FIG. 2 and toisolate cDNA and genomic clones that correspond to variants (alleles,orthologs, etc.) producing the same or related peptides shown in FIG. 2.As illustrated in FIG. 3, SNPs were identified at 47 differentnucleotide positions.

[0162] The probe can correspond to any sequence along the entire lengthof the nucleic acid molecules provided in the Figures. Accordingly, itcould be derived from 5′ noncoding regions, the coding region, and 3′noncoding regions. However, as discussed, fragments are not to beconstrued as encompassing fragments disclosed prior to the presentinvention.

[0163] The nucleic acid molecules are also useful as primers for PCR toamplify any given region of a nucleic acid molecule and are useful tosynthesize antisense molecules of desired length and sequence.

[0164] The nucleic acid molecules are also useful for constructingrecombinant vectors. Such vectors include expression vectors thatexpress a portion of, or all of, the peptide sequences. Vectors alsoinclude insertion vectors, used to integrate into another nucleic acidmolecule sequence, such as into the cellular genome, to alter in situexpression of a gene and/or gene product. For example, an endogenouscoding sequence can be replaced via homologous recombination with all orpart of the coding region containing one or more specifically introducedmutations.

[0165] The nucleic acid molecules are also useful for expressingantigenic portions of the proteins.

[0166] The nucleic acid molecules are also useful as probes fordetermining the chromosomal positions of the nucleic acid molecules bymeans of in situ hybridization methods. As indicated by the datapresented in FIG. 3, the map position was determined to be on chromosome10 by ePCR, and confirmed with radiation hybrid mapping.

[0167] The nucleic acid molecules are also useful in making vectorscontaining the gene regulatory regions of the nucleic acid molecules ofthe present invention.

[0168] The nucleic acid molecules are also useful for designingribozymes corresponding to all, or a part, of the mRNA produced from thenucleic acid molecules described herein.

[0169] The nucleic acid molecules are also useful for making vectorsthat express part, or all, of the peptides.

[0170] The nucleic acid molecules are also useful for constructing hostcells expressing a part, or all, of the nucleic acid molecules andpeptides.

[0171] The nucleic acid molecules are also useful for constructingtransgenic animals expressing all, or a part, of the nucleic acidmolecules and peptides.

[0172] The nucleic acid molecules are also useful as hybridizationprobes for determining the presence, level, form and distribution ofnucleic acid expression. Experimental data as provided in FIG. 1indicates that the transporter proteins of the present invention areexpressed in humans in the liver, adrenal gland, normal and tumorousnervous tissue, adult amygdala, brain meningioma tissue, denis-drash,adult and fetal brain, placenta, testis and kidney. Specifically, avirtual northern blot shows expression in the liver, adrenal gland,normal and tumorous nervous tissues, adult amygdala, brain meningiomatissue, and denis-drash. In addition, PCR-based tissue screening panelsindicate expression in the adult and fetal brain, placenta, testis, andkidney.

[0173] Accordingly, the probes can be used to detect the presence of, orto determine levels of, a specific nucleic acid molecule in cells,tissues, and in organisms. The nucleic acid whose level is determinedcan be DNA or RNA. Accordingly, probes corresponding to the peptidesdescribed herein can be used to assess expression and/or gene copynumber in a given cell, tissue, or organism. These uses are relevant fordiagnosis of disorders involving an increase or decrease in transporterprotein expression relative to normal results.

[0174] In vitro techniques for detection of mRNA include Northernhybridizations and in situ hybridizations. In vitro techniques fordetecting DNA include Southern hybridizations and in situ hybridization.

[0175] Probes can be used as a part of a diagnostic test kit foridentifying cells or tissues that express a transporter protein, such asby measuring a level of a transporter-encoding nucleic acid in a sampleof cells from a subject e.g., mRNA or genomic DNA, or determining if atransporter gene has been mutated. Experimental data as provided in FIG.1 indicates that the transporter proteins of the present invention areexpressed in humans in the liver, adrenal gland, normal and tumorousnervous tissue, adult amygdala, brain meningioma tissue, denis-drash,adult and fetal brain, placenta, testis and kidney. Specifically, avirtual northern blot shows expression in the liver, adrenal gland,normal and tumorous nervous tissues, adult amygdala, brain meningiomatissue, and denis-drash. In addition, PCR-based tissue screening panelsindicate expression in the adult and fetal brain, placenta, testis, andkidney.

[0176] Nucleic acid expression assays are useful for drug screening toidentify compounds that modulate transporter nucleic acid expression.

[0177] The invention thus provides a method for identifying a compoundthat can be used to treat a disorder associated with nucleic acidexpression of the transporter gene, particularly biological andpathological processes that are mediated by the transporter in cells andtissues that express it. Experimental data as provided in FIG. 1indicates expression in humans in the liver, adrenal gland, normal andtumorous nervous tissue, adult amygdala, brain meningioma tissue,denis-drash, adult and fetal brain, placenta, testis and kidney. Themethod typically includes assaying the ability of the compound tomodulate the expression of the transporter nucleic acid and thusidentifying a compound that can be used to treat a disordercharacterized by undesired transporter nucleic acid expression. Theassays can be performed in cell-based and cell-free systems. Cell-basedassays include cells naturally expressing the transporter nucleic acidor recombinant cells genetically engineered to express specific nucleicacid sequences.

[0178] The assay for transporter nucleic acid expression can involvedirect assay of nucleic acid levels, such as mRNA levels, or oncollateral compounds involved in the signal pathway. Further, theexpression of genes that are up- or down-regulated in response to thetransporter protein signal pathway can also be assayed. In thisembodiment the regulatory regions of these genes can be operably linkedto a reporter gene such as luciferase.

[0179] Thus, modulators of transporter gene expression can be identifiedin a method wherein a cell is contacted with a candidate compound andthe expression of mRNA determined. The level of expression oftransporter mRNA in the presence of the candidate compound is comparedto the level of expression of transporter mRNA in the absence of thecandidate compound. The candidate compound can then be identified as amodulator of nucleic acid expression based on this comparison and beused, for example to treat a disorder characterized by aberrant nucleicacid expression. When expression of mRNA is statistically significantlygreater in the presence of the candidate compound than in its absence,the candidate compound is identified as a stimulator of nucleic acidexpression. When nucleic acid expression is statistically significantlyless in the presence of the candidate compound than in its absence, thecandidate compound is identified as an inhibitor of nucleic acidexpression.

[0180] The invention further provides methods of treatment, with thenucleic acid as a target, using a compound identified through drugscreening as a gene modulator to modulate transporter nucleic acidexpression in cells and tissues that express the transporter.Experimental data as provided in FIG. 1 indicates that the transporterproteins of the present invention are expressed in humans in the liver,adrenal gland, normal and tumorous nervous tissue, adult amygdala, brainmeningioma tissue, denis-drash, adult and fetal brain, placenta, testisand kidney. Specifically, a virtual northern blot shows expression inthe liver, adrenal gland, normal and tumorous nervous tissues, adultamygdala, brain meningioma tissue, and denis-drash. In addition,PCR-based tissue screening panels indicate expression in the adult andfetal brain, placenta, testis, and kidney. Modulation includes bothup-regulation (i.e. activation or agonization) or down-regulation(suppression or antagonization) or nucleic acid expression.

[0181] Alternatively, a modulator for transporter nucleic acidexpression can be a small molecule or drug identified using thescreening assays described herein as long as the drug or small moleculeinhibits the transporter nucleic acid expression in the cells andtissues that express the protein. Experimental data as provided in FIG.1 indicates expression in humans in the liver, adrenal gland, normal andtumorous nervous tissue, adult amygdala, brain meningioma tissue,denis-drash, adult and fetal brain, placenta, testis and kidney.

[0182] The nucleic acid molecules are also useful for monitoring theeffectiveness of modulating compounds on the expression or activity ofthe transporter gene in clinical trials or in a treatment regimen. Thus,the gene expression pattern can serve as a barometer for the continuingeffectiveness of treatment with the compound, particularly withcompounds to which a patient can develop resistance. The gene expressionpattern can also serve as a marker indicative of a physiologicalresponse of the affected cells to the compound. Accordingly, suchmonitoring would allow either increased administration of the compoundor the administration of alternative compounds to which the patient hasnot become resistant. Similarly, if the level of nucleic acid expressionfalls below a desirable level, administration of the compound could becommensurately decreased.

[0183] The nucleic acid molecules are also useful in diagnostic assaysfor qualitative changes in transporter nucleic acid expression, andparticularly in qualitative changes that lead to pathology. The nucleicacid molecules can be used to detect mutations in transporter genes andgene expression products such as mRNA. The nucleic acid molecules can beused as hybridization probes to detect naturally occurring geneticmutations in the transporter gene and thereby to determine whether asubject with the mutation is at risk for a disorder caused by themutation. Mutations include deletion, addition, or substitution of oneor more nucleotides in the gene, chromosomal rearrangement, such asinversion or transposition, modification of genomic DNA, such asaberrant methylation patterns or changes in gene copy number, such asamplification. Detection of a mutated form of the transporter geneassociated with a dysfunction provides a diagnostic tool for an activedisease or susceptibility to disease when the disease results fromoverexpression, underexpression, or altered expression of a transporterprotein.

[0184] Individuals carrying mutations in the transporter gene can bedetected at the nucleic acid level by a variety of techniques. FIG. 3provides information on SNPs that have been found in the gene encodingthe transporter protein of the present invention. SNPs were identifiedat 47 different nucleotide positions. Changes in the amino acid sequencecaused by these SNPs is indicated in FIG. 3 and can readily bedetermined using the universal genetic code and the protein sequenceprovided in FIG. 2 as a reference. Some of these SNPs that are locatedoutside the ORF and in introns may affect gene transcription. Asindicated by the data presented in FIG. 3, the map position wasdetermined to be on chromosome 10 by ePCR, and confirmed with radiationhybrid mapping. Genomic DNA can be analyzed directly or can be amplifiedby using PCR prior to analysis. RNA or cDNA can be used in the same way.In some uses, detection of the mutation involves the use of aprobe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Pat.Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or,alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegranet al., Science 241:1077-1080 (1988); and Nakazawa et al., PNAS91:360-364 (1994)), the latter of which can be particularly useful fordetecting point mutations in the gene (see Abravaya et al., NucleicAcids Res. 23:675-682 (1995)). This method can include the steps ofcollecting a sample of cells from a patient, isolating nucleic acid(e.g., genomic, mRNA or both) from the cells of the sample, contactingthe nucleic acid sample with one or more primers which specificallyhybridize to a gene under conditions such that hybridization andamplification of the gene (if present) occurs, and detecting thepresence or absence of an amplification product, or detecting the sizeof the amplification product and comparing the length to a controlsample. Deletions and insertions can be detected by a change in size ofthe amplified product compared to the normal genotype. Point mutationscan be identified by hybridizing amplified DNA to normal RNA orantisense DNA sequences.

[0185] Alternatively, mutations in a transporter gene can be directlyidentified, for example, by alterations in restriction enzyme digestionpatterns determined by gel electrophoresis.

[0186] Further, sequence-specific ribozymes (U.S. Pat. No. 5,498,531)can be used to score for the presence of specific mutations bydevelopment or loss of a ribozyme cleavage site. Perfectly matchedsequences can be distinguished from mismatched sequences by nucleasecleavage digestion assays or by differences in melting temperature.

[0187] Sequence changes at specific locations can also be assessed bynuclease protection assays such as RNase and S1 protection or thechemical cleavage method. Furthermore, sequence differences between amutant transporter gene and a wild-type gene can be determined by directDNA sequencing. A variety of automated sequencing procedures can beutilized when performing the diagnostic assays (Naeve, C. W., (1995)Biotechniques 19:448), including sequencing by mass spectrometry (see,e.g., PCT International Publication No. WO 94/16101; Cohen et al, Adv.Chromatogr. 36:127-162 (1996); and Griffin et al., Appl. Biochem.Biotechnol. 38:147-159 (1993)).

[0188] Other methods for detecting mutations in the gene include methodsin which protection from cleavage agents is used to detect mismatchedbases in RNA/RNA or RNA/DNA duplexes (Myers et al., Science 230:1242(1985)); Cotton et al., PNAS 85:4397 (1988); Saleeba et al., Meth.Enzymol. 217:286-295 (1992)), electrophoretic mobility of mutant andwild type nucleic acid is compared (Orita et al., PNAS 86:2766 (1989);Cotton et al., Mutat. Res. 285:125-144 (1993); and Hayashi et al.,Genet. Anal. Tech. Appl. 9:73-79 (1992)), and movement of mutant orwild-type fragments in polyacrylamide gels containing a gradient ofdenaturant is assayed using denaturing gradient gel electrophoresis(Myers et al., Nature 313:495 (1985)). Examples of other techniques fordetecting point mutations include selective oligonucleotidehybridization, selective amplification, and selective primer extension.

[0189] The nucleic acid molecules are also useful for testing anindividual for a genotype that while not necessarily causing thedisease, nevertheless affects the treatment modality. Thus, the nucleicacid molecules can be used to study the relationship between anindividual's genotype and the individual's response to a compound usedfor treatment (pharmacogenomic relationship). Accordingly, the nucleicacid molecules described herein can be used to assess the mutationcontent of the transporter gene in an individual in order to select anappropriate compound or dosage regimen for treatment. FIG. 3 providesinformation on SNPs that have been found in the gene encoding thetransporter protein of the present invention. SNPs were identified at 47different nucleotide positions. Changes in the amino acid sequencecaused by these SNPs is indicated in FIG. 3 and can readily bedetermined using the universal genetic code and the protein sequenceprovided in FIG. 2 as a reference. Some of these SNPs that are locatedoutside the ORF and in introns may affect gene transcription.

[0190] Thus nucleic acid molecules displaying genetic variations thataffect treatment provide a diagnostic target that can be used to tailortreatment in an individual. Accordingly, the production of recombinantcells and animals containing these polymorphisms allow effectiveclinical design of treatment compounds and dosage regimens.

[0191] The nucleic acid molecules are thus useful as antisenseconstructs to control transporter gene expression in cells, tissues, andorganisms. A DNA antisense nucleic acid molecule is designed to becomplementary to a region of the gene involved in transcription,preventing transcription and hence production of transporter protein. Anantisense RNA or DNA nucleic acid molecule would hybridize to the mRNAand thus block translation of mRNA into transporter protein.

[0192] Alternatively, a class of antisense molecules can be used toinactivate mRNA in order to decrease expression of transporter nucleicacid. Accordingly, these molecules can treat a disorder characterized byabnormal or undesired transporter nucleic acid expression. Thistechnique involves cleavage by means of ribozymes containing nucleotidesequences complementary to one or more regions in the mRNA thatattenuate the ability of the mRNA to be translated. Possible regionsinclude coding regions and particularly coding regions corresponding tothe catalytic and other functional activities of the transporterprotein, such as ligand binding.

[0193] The nucleic acid molecules also provide vectors for gene therapyin patients containing cells that are aberrant in transporter geneexpression. Thus, recombinant cells, which include the patient's cellsthat have been engineered ex vivo and returned to the patient, areintroduced into an individual where the cells produce the desiredtransporter protein to treat the individual.

[0194] The invention also encompasses kits for detecting the presence ofa transporter nucleic acid in a biological sample. Experimental data asprovided in FIG. 1 indicates that the transporter proteins of thepresent invention are expressed in humans in the liver, adrenal gland,normal and tumorous nervous tissue, adult amygdala, brain meningiomatissue, denis-drash, adult and fetal brain, placenta, testis and kidney.Specifically, a virtual northern blot shows expression in the liver,adrenal gland, normal and tumorous nervous tissues, adult amygdala,brain meningioma tissue, and denis-drash. In addition, PCR-based tissuescreening panels indicate expression in the adult and fetal brain,placenta, testis, and kidney. For example, the kit can comprise reagentssuch as a labeled or labelable nucleic acid or agent capable ofdetecting transporter nucleic acid in a biological sample; means fordetermining the amount of transporter nucleic acid in the sample; andmeans for comparing the amount of transporter nucleic acid in the samplewith a standard. The compound or agent can be packaged in a suitablecontainer. The kit can further comprise instructions for using the kitto detect transporter protein mRNA or DNA.

[0195] Nucleic Acid Arrays

[0196] The present invention further provides nucleic acid detectionkits, such as arrays or microarrays of nucleic acid molecules that arebased on the sequence information provided in FIGS. 1 and 3 (SEQ IDNOS:1 and 3).

[0197] As used herein “Arrays” or “Microarrays” refers to an array ofdistinct polynucleotides or oligonucleotides synthesized on a substrate,such as paper, nylon or other type of membrane, filter, chip, glassslide, or any other suitable solid support. In one embodiment, themicroarray is prepared and used according to the methods described inU.S. Pat. No. 5,837,832, Chee et al, PCT application W095/11995 (Chee etal.), Lockhart, D. J. et al. (1996; Nat. Biotech. 14: 1675-1680) andSchena, M. et al. (1996; Proc. Natl. Acad. Sci. 93: 10614-10619), all ofwhich are incorporated herein in their entirety by reference. In otherembodiments, such arrays are produced by the methods described by Brownet al., U.S. Pat. No. 5,807,522.

[0198] The microarray or detection kit is preferably composed of a largenumber of unique, single-stranded nucleic acid sequences, usually eithersynthetic antisense oligonucleotides or fragments of cDNAs, fixed to asolid support. The oligonucleotides are preferably about 6-60nucleotides in length, more preferably 15-30 nucleotides in length, andmost preferably about 20-25 nucleotides in length. For a certain type ofmicroarray or detection kit, it may be preferable to useoligonucleotides that are only 7-20 nucleotides in length. Themicroarray or detection kit may contain oligonucleotides that cover theknown 5′, or 3′, sequence, sequential oligonucleotides that cover thefull length sequence; or unique oligonucleotides selected fromparticular areas along the length of the sequence. Polynucleotides usedin the microarray or detection kit may be oligonucleotides that arespecific to a gene or genes of interest.

[0199] In order to produce oligonucleotides to a known sequence for amicroarray or detection kit, the gene(s) of interest (or an ORFidentified from the contigs of the present invention) is typicallyexamined using a computer algorithm which starts at the 5′ or at the 3′end of the nucleotide sequence. Typical algorithms will then identifyoligomers of defined length that are unique to the gene, have a GCcontent within a range suitable for hybridization, and lack predictedsecondary structure that may interfere with hybridization. In certainsituations it may be appropriate to use pairs of oligonucleotides on amicroarray or detection kit. The “pairs” will be identical, except forone nucleotide that preferably is located in the center of the sequence.The second oligonucleotide in the pair (mismatched by one) serves as acontrol. The number of oligonucleotide pairs may range from two to onemillion. The oligomers are synthesized at designated areas on asubstrate using a light-directed chemical process. The substrate may bepaper, nylon or other type of membrane, filter, chip, glass slide or anyother suitable solid support.

[0200] In another aspect, an oligonucleotide may be synthesized on thesurface of the substrate by using a chemical coupling procedure and anink jet application apparatus, as described in PCT applicationW095/251116 (Baldeschweiler et al.) which is incorporated herein in itsentirety by reference. In another aspect, a “gridded” array analogous toa dot (or slot) blot may be used to arrange and link cDNA fragments oroligonucleotides to the surface of a substrate using a vacuum system,thermal, UV, mechanical or chemical bonding procedures. An array, suchas those described above, may be produced by hand or by using availabledevices (slot blot or dot blot apparatus), materials (any suitable solidsupport), and machines (including robotic instruments), and may contain8, 24, 96, 384, 1536, 6144 or more oligonucleotides, or any other numberbetween two and one million which lends itself to the efficient use ofcommercially available instrumentation.

[0201] In order to conduct sample analysis using a microarray ordetection kit, the RNA or DNA from a biological sample is made intohybridization probes. The mRNA is isolated, and cDNA is produced andused as a template to make antisense RNA (aRNA). The aRNA is amplifiedin the presence of fluorescent nucleotides, and labeled probes areincubated with the microarray or detection kit so that the probesequences hybridize to complementary oligonucleotides of the microarrayor detection kit. Incubation conditions are adjusted so thathybridization occurs with precise complementary matches or with variousdegrees of less complementarity. After removal of nonhybridized probes,a scanner is used to determine the levels and patterns of fluorescence.The scanned images are examined to determine degree of complementarityand the relative abundance of each oligonucleotide sequence on themicroarray or detection kit. The biological samples may be obtained fromany bodily fluids (such as blood, urine, saliva, phlegm, gastric juices,etc.) cultured cells, biopsies, or other tissue preparations. Adetection system may be used to measure the absence, presence, andamount of hybridization for all of the distinct sequencessimultaneously. This data may be used for large-scale correlationstudies on the sequences, expression patterns, mutations, variants, orpolymorphisms among samples.

[0202] Using such arrays, the present invention provides methods toidentify the expression of the transporter proteins/peptides of thepresent invention. In detail, such methods comprise incubating a testsample with one or more nucleic acid molecules and assaying for bindingof the nucleic acid molecule with components within the test sample.Such assays will typically involve arrays comprising many genes, atleast one of which is a gene of the present invention and or alleles ofthe transporter gene of the present invention. FIG. 3 providesinformation on SNPs that have been found in the gene encoding thetransporter protein of the present invention. SNPs were identified at 47different nucleotide positions. Changes in the amino acid sequencecaused by these SNPs is indicated in FIG. 3 and can readily bedetermined using the universal genetic code and the protein sequenceprovided in FIG. 2 as a reference. Some of these SNPs that are locatedoutside the ORF and in introns may affect gene transcription.

[0203] Conditions for incubating a nucleic acid molecule with a testsample vary. Incubation conditions depend on the format employed in theassay, the detection methods employed, and the type and nature of thenucleic acid molecule used in the assay. One skilled in the art willrecognize that any one of the commonly available hybridization,amplification or array assay formats can readily be adapted to employthe novel fragments of the Human genome disclosed herein. Examples ofsuch assays can be found in Chard, T, An Introduction toRadioimmunoassay and Related Techniques, Elsevier Science Publishers,Amsterdam, The Netherlands (1986); Bullock, G. R. et al., Techniques inImmunocytochemistry, Academic Press, Orlando, Fla. Vol. 1 (1982), Vol. 2(1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of EnzymeImmunoassays: Laboratory Techniques in Biochemistry and MolecularBiology, Elsevier Science Publishers, Amsterdam, The Netherlands (1985).

[0204] The test samples of the present invention include cells, proteinor membrane extracts of cells. The test sample used in theabove-described method will vary based on the assay format, nature ofthe detection method and the tissues, cells or extracts used as thesample to be assayed. Methods for preparing nucleic acid extracts or ofcells are well known in the art and can be readily be adapted in orderto obtain a sample that is compatible with the system utilized.

[0205] In another embodiment of the present invention, kits are providedwhich contain the necessary reagents to carry out the assays of thepresent invention.

[0206] Specifically, the invention provides a compartmentalized kit toreceive, in close confinement, one or more containers which comprises:(a) a first container comprising one of the nucleic acid molecules thatcan bind to a fragment of the Human genome disclosed herein; and (b) oneor more other containers comprising one or more of the following: washreagents, reagents capable of detecting presence of a bound nucleicacid.

[0207] In detail, a compartmentalized kit includes any kit in whichreagents are contained in separate containers. Such containers includesmall glass containers, plastic containers, strips of plastic, glass orpaper, or arraying material such as silica. Such containers allows oneto efficiently transfer reagents from one compartment to anothercompartment such that the samples and reagents are notcross-contaminated, and the agents or solutions of each container can beadded in a quantitative fashion from one compartment to another. Suchcontainers will include a container which will accept the test sample, acontainer which contains the nucleic acid probe, containers whichcontain wash reagents (such as phosphate buffered saline, Tris-buffers,etc.), and containers which contain the reagents used to detect thebound probe. One skilled in the art will readily recognize that thepreviously unidentified transporter gene of the present invention can beroutinely identified using the sequence information disclosed herein canbe readily incorporated into one of the established kit formats whichare well known in the art, particularly expression arrays.

[0208] Vectors/host cells

[0209] The invention also provides vectors containing the nucleic acidmolecules described herein. The term “vector” refers to a vehicle,preferably a nucleic acid molecule, which can transport the nucleic acidmolecules. When the vector is a nucleic acid molecule, the nucleic acidmolecules are covalently linked to the vector nucleic acid. With thisaspect of the invention, the vector includes a plasmid, single or doublestranded phage, a single or double stranded RNA or DNA viral vector, orartificial chromosome, such as a BAC, PAC, YAC, OR MAC.

[0210] A vector can be maintained in the host cell as anextrachromosomal element where it replicates and produces additionalcopies of the nucleic acid molecules. Alternatively, the vector mayintegrate into the host cell genome and produce additional copies of thenucleic acid molecules when the host cell replicates.

[0211] The invention provides vectors for the maintenance (cloningvectors) or vectors for expression (expression vectors) of the nucleicacid molecules. The vectors can function in procaryotic or eukaryoticcells or in both (shuttle vectors).

[0212] Expression vectors contain cis-acting regulatory regions that areoperably linked in the vector to the nucleic acid molecules such thattranscription of the nucleic acid molecules is allowed in a host cell.The nucleic acid molecules can be introduced into the host cell with aseparate nucleic acid molecule capable of affecting transcription. Thus,the second nucleic acid molecule may provide a trans-acting factorinteracting with the cis-regulatory control region to allowtranscription of the nucleic acid molecules from the vector.Alternatively, a trans-acting factor may be supplied by the host cell.Finally, a trans-acting factor can be produced from the vector itself.It is understood, however, that in some embodiments, transcriptionand/or translation of the nucleic acid molecules can occur in acell-free system.

[0213] The regulatory sequence to which the nucleic acid moleculesdescribed herein can be operably linked include promoters for directingmRNA transcription. These include, but are not limited to, the leftpromoter from bacteriophage λ, the lac, TRP, and TAC promoters from E.coli, the early and late promoters from SV40, the CMV immediate earlypromoter, the adenovirus early and late promoters, and retroviruslong-terminal repeats.

[0214] In addition to control regions that promote transcription,expression vectors may also include regions that modulate transcription,such as repressor binding sites and enhancers. Examples include the SV40enhancer, the cytomegalovirus immediate early enhancer, polyomaenhancer, adenovirus enhancers, and retrovirus LTR enhancers.

[0215] In addition to containing sites for transcription initiation andcontrol, expression vectors can also contain sequences necessary fortranscription termination and, in the transcribed region a ribosomebinding site for translation. Other regulatory control elements forexpression include initiation and termination codons as well aspolyadenylation signals. The person of ordinary skill in the art wouldbe aware of the numerous regulatory sequences that are useful inexpression vectors. Such regulatory sequences are described, forexample, in Sambrook et al., Molecular Cloning: A Laboratory Manual.2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.,(1989).

[0216] A variety of expression vectors can be used to express a nucleicacid molecule. Such vectors include chromosomal, episomal, andvirus-derived vectors, for example vectors derived from bacterialplasmids, from bacteriophage, from yeast episomes, from yeastchromosomal elements, including yeast artificial chromosomes, fromviruses such as baculoviruses, papovaviruses such as SV40, Vacciniaviruses, adenoviruses, poxviruses, pseudorabies viruses, andretroviruses. Vectors may also be derived from combinations of thesesources such as those derived from plasmid and bacteriophage geneticelements, e.g. cosmids and phagemids. Appropriate cloning and expressionvectors for prokaryotic and eukaryotic hosts are described in Sambrooket al., Molecular Cloning: A Laboratory Manual. 2nd ed., Cold SpringHarbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

[0217] The regulatory sequence may provide constitutive expression inone or more host cells (i.e. tissue specific) or may provide forinducible expression in one or more cell types such as by temperature,nutrient additive, or exogenous factor such as a hormone or otherligand. A variety of vectors providing for constitutive and inducibleexpression in prokaryotic and eukaryotic hosts are well known to thoseof ordinary skill in the art.

[0218] The nucleic acid molecules can be inserted into the vectornucleic acid by well-known methodology. Generally, the DNA sequence thatwill ultimately be expressed is joined to an expression vector bycleaving the DNA sequence and the expression vector with one or morerestriction enzymes and then ligating the fragments together. Proceduresfor restriction enzyme digestion and ligation are well known to those ofordinary skill in the art.

[0219] The vector containing the appropriate nucleic acid molecule canbe introduced into an appropriate host cell for propagation orexpression using well-known techniques. Bacterial cells include, but arenot limited to, E. coli, Streptomyces, and Salmonella typhimurium.Eukaryotic cells include, but are not limited to, yeast, insect cellssuch as Drosophila, animal cells such as COS and CHO cells, and plantcells.

[0220] As described herein, it may be desirable to express the peptideas a fusion protein. Accordingly, the invention provides fusion vectorsthat allow for the production of the peptides. Fusion vectors canincrease the expression of a recombinant protein, increase thesolubility of the recombinant protein, and aid in the purification ofthe protein by acting for example as a ligand for affinity purification.A proteolytic cleavage site may be introduced at the junction of thefusion moiety so that the desired peptide can ultimately be separatedfrom the fusion moiety. Proteolytic enzymes include, but are not limitedto, factor Xa, thrombin, and enterotransporter. Typical fusionexpression vectors include pGEX (Smith et al., Gene 67:31-40 (1988)),pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia,Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose Ebinding protein, or protein A, respectively, to the target recombinantprotein. Examples of suitable inducible non-fusion E. coli expressionvectors include pTrc (Amann et al., Gene 69:301-315 (1988)) and pET 11d(Studier et al., Gene Expression Technology: Methods in Enzymology185:60-89 (1990)).

[0221] Recombinant protein expression can be maximized in host bacteriaby providing a genetic background wherein the host cell has an impairedcapacity to proteolytically cleave the recombinant protein. (Gottesman,S., Gene Expression Technology: Methods in Enzymology 185, AcademicPress, San Diego, Calif. (1990)119-128). Alternatively, the sequence ofthe nucleic acid molecule of interest can be altered to providepreferential codon usage for a specific host cell, for example E. coli.(Wada et al., Nucleic Acids Res. 20:2111-2118 (1992)).

[0222] The nucleic acid molecules can also be expressed by expressionvectors that are operative in yeast. Examples of vectors for expressionin yeast e.g., S. cerevisiae include pYepSec1 (Baldari, et al., EMBO J.6:229-234 (1987)), pMFa (Kurjan et al., Cell 30:933-943(1982)), pJRY88(Schultz et al., Gene 54:113-123 (1987)), and pYES2 (InvitrogenCorporation, San Diego, Calif.).

[0223] The nucleic acid molecules can also be expressed in insect cellsusing, for example, baculovirus expression vectors. Baculovirus vectorsavailable for expression of proteins in cultured insect cells (e.g., Sf9cells) include the pAc series (Smith et al., Mol. Cell Biol. 3:2156-2165(1983)) and the pVL series (Lucklow et al., Virology 170:31-39 (1989)).

[0224] In certain embodiments of the invention, the nucleic acidmolecules described herein are expressed in mammalian cells usingmammalian expression vectors. Examples of mammalian expression vectorsinclude pCDM8 (Seed, B. Nature 329:840(1987)) and pMT2PC (Kaufman etal., EMBO J. 6:187-195 (1987)).

[0225] The expression vectors listed herein are provided by way ofexample only of the well-known vectors available to those of ordinaryskill in the art that would be useful to express the nucleic acidmolecules. The person of ordinary skill in the art would be aware ofother vectors suitable for maintenance propagation or expression of thenucleic acid molecules described herein. These are found for example inSambrook, J., Fritsh, E. F., and Maniatis, T. Molecular Cloning: ALaboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold SpringHarbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.

[0226] The invention also encompasses vectors in which the nucleic acidsequences described herein are cloned into the vector in reverseorientation, but operably linked to a regulatory sequence that permitstranscription of antisense RNA. Thus, an antisense transcript can beproduced to all, or to a portion, of the nucleic acid molecule sequencesdescribed herein, including both coding and non-coding regions.Expression of this antisense RNA is subject to each of the parametersdescribed above in relation to expression of the sense RNA (regulatorysequences, constitutive or inducible expression, tissue-specificexpression).

[0227] The invention also relates to recombinant host cells containingthe vectors described herein. Host cells therefore include prokaryoticcells, lower eukaryotic cells such as yeast, other eukaryotic cells suchas insect cells, and higher eukaryotic cells such as mammalian cells.

[0228] The recombinant host cells are prepared by introducing the vectorconstructs described herein into the cells by techniques readilyavailable to the person of ordinary skill in the art. These include, butare not limited to, calcium phosphate transfection,DEAE-dextran-mediated transfection, cationic lipid-mediatedtransfection, electroporation, transduction, infection, lipofection, andother techniques such as those found in Sambrook, et al. (MolecularCloning: A Laboratory Manual. 2nd, ed, Cold Spring Harbor Laboratory,Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989).

[0229] Host cells can contain more than one vector. Thus, differentnucleotide sequences can be introduced on different vectors of the samecell. Similarly, the nucleic acid molecules can be introduced eitheralone or with other nucleic acid molecules that are not related to thenucleic acid molecules such as those providing trans-acting factors forexpression vectors. When more than one vector is introduced into a cell,the vectors can be introduced independently, co-introduced or joined tothe nucleic acid molecule vector.

[0230] In the case of bacteriophage and viral vectors, these can beintroduced into cells as packaged or encapsulated virus by standardprocedures for infection and transduction. Viral vectors can bereplication-competent or replication-defective. In the case in whichviral replication is defective, replication will occur in host cellsproviding functions that complement the defects.

[0231] Vectors generally include selectable markers that enable theselection of the subpopulation of cells that contain the recombinantvector constructs. The marker can be contained in the same vector thatcontains the nucleic acid molecules described herein or may be on aseparate vector. Markers include tetracycline or ampicillin-resistancegenes for prokaryotic host cells and dihydrofolate reductase or neomycinresistance for eukaryotic host cells. However, any marker that providesselection for a phenotypic trait will be effective.

[0232] While the mature proteins can be produced in bacteria, yeast,mammalian cells, and other cells under the control of the appropriateregulatory sequences, cell-free transcription and translation systemscan also be used to produce these proteins using RNA derived from theDNA constructs described herein.

[0233] Where secretion of the peptide is desired, which is difficult toachieve with multi-transmembrane domain containing proteins such astransporters, appropriate secretion signals are incorporated into thevector. The signal sequence can be endogenous to the peptides orheterologous to these peptides.

[0234] Where the peptide is not secreted into the medium, which istypically the case with transporters, the protein can be isolated fromthe host cell by standard disruption procedures, including freeze thaw,sonication, mechanical disruption, use of lysing agents and the like.The peptide can then be recovered and purified by well-knownpurification methods including ammonium sulfate precipitation, acidextraction, anion or cationic exchange chromatography, phosphocellulosechromatography, hydrophobic-interaction chromatography, affinitychromatography, hydroxylapatite chromatography, lectin chromatography,or high performance liquid chromatography.

[0235] It is also understood that depending upon the host cell inrecombinant production of the peptides described herein, the peptidescan have various glycosylation patterns, depending upon the cell, ormaybe non-glycosylated as when produced in bacteria. In addition, thepeptides may include an initial modified methionine in some cases as aresult of a host-mediated process.

[0236] Uses of vectors and host cells

[0237] The recombinant host cells expressing the peptides describedherein have a variety of uses. First, the cells are useful for producinga transporter protein or peptide that can be further purified to producedesired amounts of transporter protein or fragments. Thus, host cellscontaining expression vectors are useful for peptide production.

[0238] Host cells are also useful for conducting cell-based assaysinvolving the transporter protein or transporter protein fragments, suchas those described above as well as other formats known in the art.Thus, a recombinant host cell expressing a native transporter protein isuseful for assaying compounds that stimulate or inhibit transporterprotein function.

[0239] Host cells are also useful for identifying transporter proteinmutants in which these functions are affected. If the mutants naturallyoccur and give rise to a pathology, host cells containing the mutationsare useful to assay compounds that have a desired effect on the mutanttransporter protein (for example, stimulating or inhibiting function)which may not be indicated by their effect on the native transporterprotein.

[0240] Genetically engineered host cells can be further used to producenon-human transgenic animals. A transgenic animal is preferably amammal, for example a rodent, such as a rat or mouse, in which one ormore of the cells of the animal include a transgene. A transgene isexogenous DNA that is integrated into the genome of a cell from which atransgenic animal develops and which remains in the genome of the matureanimal in one or more cell types or tissues of the transgenic animal.These animals are useful for studying the function of a transporterprotein and identifying and evaluating modulators of transporter proteinactivity. Other examples of transgenic animals include non-humanprimates, sheep, dogs, cows, goats, chickens, and amphibians.

[0241] A transgenic animal can be produced by introducing nucleic acidinto the male pronuclei of a fertilized oocyte, e.g., by microinjection,retroviral infection, and allowing the oocyte to develop in apseudopregnant female foster animal. Any of the transporter proteinnucleotide sequences can be introduced as a transgene into the genome ofa non-human animal, such as a mouse.

[0242] Any of the regulatory or other sequences useful in expressionvectors can form part of the transgenic sequence. This includes intronicsequences and polyadenylation signals, if not already included. Atissue-specific regulatory sequence(s) can be operably linked to thetransgene to direct expression of the transporter protein to particularcells.

[0243] Methods for generating transgenic animals via embryo manipulationand microinjection, particularly animals such as mice, have becomeconventional in the art and are described, for example, in U.S. Pat.Nos. 4,736,866 and 4,870,009, both by Leder et al., U.S. Pat. No.4,873,191 by Wagner et al. and in Hogan, B., Manipulating the MouseEmbryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.,1986). Similar methods are used for production of other transgenicanimals. A transgenic founder animal can be identified based upon thepresence of the transgene in its genome and/or expression of transgenicmRNA in tissues or cells of the animals. A transgenic founder animal canthen be used to breed additional animals carrying the transgene.Moreover, transgenic animals carrying a transgene can further be bred toother transgenic animals carrying other transgenes. A transgenic animalalso includes animals in which the entire animal or tissues in theanimal have been produced using the homologously recombinant host cellsdescribed herein.

[0244] In another embodiment, transgenic non-human animals can beproduced which contain selected systems that allow for regulatedexpression of the transgene. One example of such a system is thecre/loxp recombinase system of bacteriophage P1. For a description ofthe cre/loxP recombinase system, see, e.g., Lakso et al. PNAS89:6232-6236 (1992). Another example of a recombinase system is the FLPrecombinase system of S. cerevisiae (O'Gorman et al. Science251:1351-1355 (1991). If a cre/loxP recombinase system is used toregulate expression of the transgene, animals containing transgenesencoding both the Cre recombinase and a selected protein is required.Such animals can be provided through the construction of “double”transgenic animals, e.g., by mating two transgenic animals, onecontaining a transgene encoding a selected protein and the othercontaining a transgene encoding a recombinase.

[0245] Clones of the non-human transgenic animals described herein canalso be produced according to the methods described in Wilmut, I. et al.Nature 385:810-813 (1997) and PCT International Publication Nos. WO97/07668 and WO 97/07669. In brief, a cell, e.g., a somatic cell, fromthe transgenic animal can be isolated and induced to exit the growthcycle and enter G₀ phase. The quiescent cell can then be fused, e.g.,through the use of electrical pulses, to an enucleated oocyte from ananimal of the same species from which the quiescent cell is isolated.The reconstructed oocyte is then cultured such that it develops tomorula or blastocyst and then transferred to pseudopregnant femalefoster animal. The offspring born of this female foster animal will be aclone of the animal from which the cell, e.g., the somatic cell, isisolated.

[0246] Transgenic animals containing recombinant cells that express thepeptides described herein are useful to conduct the assays describedherein in an in vivo context. Accordingly, the various physiologicalfactors that are present in vivo and that could effect ligand binding,transporter protein activation, and signal transduction, may not beevident from in vitro cell-free or cell-based assays. Accordingly, it isuseful to provide non-human transgenic animals to assay in vivotransporter protein function, including ligand interaction, the effectof specific mutant transporter proteins on transporter protein functionand ligand interaction, and the effect of chimeric transporter proteins.It is also possible to assess the effect of null mutations, that ismutations that substantially or completely eliminate one or moretransporter protein functions.

[0247] All publications and patents mentioned in the above specificationare herein incorporated by reference. Various modifications andvariations of the described method and system of the invention will beapparent to those skilled in the art without departing from the scopeand spirit of the invention. Although the invention has been describedin connection with specific preferred embodiments, it should beunderstood that the invention as claimed should not be unduly limited tosuch specific embodiments. Indeed, various modifications of theabove-described modes for carrying out the invention which are obviousto those skilled in the field of molecular biology or related fields areintended to be within the scope of the following claims.

1 5 1 3168 DNA HUMAN 1 ttgctcctgt gaagaaaatt cctgctggag tgctgggcgcacttggaatt ggtctagcat 60 gcttgacaga cgccttatag ctcctcaaac taaatacattattcctgggg gttcggcaga 120 ctcctacact agccgtccat ccgattccga tgtatctctggaggaggacc gggaggcagt 180 gcgcagagaa gcggagcggc aggcccaggc acagttggaaaaagcaagga caaagtccgt 240 tgcatttgcg gttcggacaa atgtcagcta cagtgcggcccatgaagatg atgttccagt 300 gcctggcatg gccatctcat tcgaagcaaa agattttctgcatgttaagg aaaaatttaa 360 caatgactgg tggatagggc gattggtaaa agaaggctgtgaaatcggat tcattccaag 420 cccagtcaaa ctagaaaaca tgaggctgca gcatgaacagagagccaagc aagggaaatt 480 ctactccagt aaatcaggag gaaattcatc atccagtttgggtgacatag tacctagttc 540 cagaaaatca acacctccat catctgctaa gcagaagcagaaatcgacag agcacactcc 600 tccgtatgat gtggtacctt ccatgcgacc agtggtcctagtgggccctt ctctgaaggg 660 ctacgaggtc acagatatga tgcaaaaagc gctgtttgattttttaaaac acagatttga 720 agggcggata tccatcacaa gggtcaccgc tgacatctcgcttgccaaac gctcggtatt 780 aaacaatccc agtaagcacg caataataga aagatccaacacaaggtcaa gcttagcgga 840 agttcagagt gaaatcgaaa ggatttttga acttgcaagaacattgcagt tggtggtcct 900 tgacgcggat acaattaatc atccagctca actcagtaaaacctccttgg cccctattat 960 agtatatgta aagatttctt ctcctaaggt tttacaaaggttaataaaat ctcgagggaa 1020 atctcaagct aaacacctca acgtccagat ggtagcagctgataaactgg ctcagtgtcc 1080 tccagagctg ttcgatgtga tcttggatga gaaccagcttgaggatgcct gtgagcacct 1140 tgccgactat ctggaggcct actggaaggc cacccatcctcccagcagta gcctccccaa 1200 ccctctcctt agccgtacat tagccacttc aagtctgcctcttagcccca ccctagcctc 1260 taattcacag ggttctcaag gtgatcagag gactgatcgctccgctccta tccgttctgc 1320 ttcccaagct gaagaagaac ctagtgtgga accagtcaagaaatcccagc accgctcttc 1380 ctcctcagcc ccacaccaca accatcgcag tgggacaagtcgcggcctct ccaggcaaga 1440 gacatttgac tcggaaaccc aggagagtcg agactctgcctacgtagagc caaaggaaga 1500 ttattcccat gaccacgtgg accactatgc ctcacaccgtgaccacaacc acagagacga 1560 gacccacggg agcagtgacc acagacacag ggagtcccggcaccgttccc gggacgtgga 1620 tcgagagcag gaccacaacg agtgcaacaa gcagcgcagccgtcataaat ccaaggatcg 1680 ctactgtgag aaggatggag aagtgatatc aaaaaaacggaatgaggctg gggaggggaa 1740 cagggatgtt tacatccgcc aatgagtttt gcccttttgtgttttttttt ttttgaagtc 1800 ttgtataact aacagcatcc ccaaaacaaa aagtctttggggtctacact gcaatcatat 1860 gtgatctgtc ttgtaatatt ttgtattatt gctgttgcttgaatagcaat agcatggata 1920 gagtattgag atactttttc ttttgtaagt gctacataaattggcctggt atggctgcag 1980 tcctccggtt gcatactgga ctcttcaaaa actgttttgggtagctgccg cttgaacaaa 2040 atctgttgcc acccaggtga tgttagtgtt ttaagaaatgtagttgatgt atccaacaag 2100 ccagaatcag cacagataaa aagtggaatt tctttgtttctccagatttt taatacgtta 2160 atacgcaggc atctgatttg catattcatt catggaccactgtttcttgc ttgtacctct 2220 ggctgactaa atttggggac agattcagtc ttgccttacacaaaggggat cataaagtta 2280 gaatctattt tctatgtact agtactgtgt actgtatagacagtttgtaa atgttatttc 2340 tgcaaacaaa caccttctta ttatatataa tatatatatatatatatcag tttgatcaca 2400 ctattttaga gtcttaatgc caagtcagca gatttgctttatgaattaca gggactagaa 2460 atgcccacat tcaggaaatt tgtaataaca ttgtctagacacctatcctc attctagtag 2520 aaagtgtgta catactgtaa atatgtgtga ttgcttgacttgaaaaggtt tgaattctga 2580 atgttatacc atccttgtaa gtaagtttgt aatttccaccataaattatg gtaaatataa 2640 aactccagag gttgctctac tccatacagt tcacactgattgtgacacat tcttagtagc 2700 tagtgtctgt tctagtcact gcactggagt ctacgagccggaactcgcta tatgcacgtg 2760 tgtgtgtccg tatgtaagaa agtgtgcacc gagtgactgaatggttgaga tgaattggaa 2820 tgctgaagac taacgaagaa actagagact gatatcgagcattctgccca cctcgctctg 2880 tatttaatta attgtgctat atgttgcttt aacaacccattgagcagtca gggaatgtga 2940 gtaagcttgc tgccgaaggt aactaggaaa gcattcatctgctgcctcct tgtttttgct 3000 cctagagagt gaaaatacag gcaattttac tgtgagtgtttcactggaaa tgtacaatct 3060 ttgtgtgtta gagtatttgt tttagtaaga aatgtttgtttacacagctt gtggaattat 3120 ttcgtggata aataaatttt tataacttct cccacttcaatttctaac 3168 2 568 PRT HUMAN 2 Met Leu Asp Arg Arg Leu Ile Ala Pro GlnThr Lys Tyr Ile Ile Pro 1 5 10 15 Gly Gly Ser Ala Asp Ser Tyr Thr SerArg Pro Ser Asp Ser Asp Val 20 25 30 Ser Leu Glu Glu Asp Arg Glu Ala ValArg Arg Glu Ala Glu Arg Gln 35 40 45 Ala Gln Ala Gln Leu Glu Lys Ala ArgThr Lys Ser Val Ala Phe Ala 50 55 60 Val Arg Thr Asn Val Ser Tyr Ser AlaAla His Glu Asp Asp Val Pro 65 70 75 80 Val Pro Gly Met Ala Ile Ser PheGlu Ala Lys Asp Phe Leu His Val 85 90 95 Lys Glu Lys Phe Asn Asn Asp TrpTrp Ile Gly Arg Leu Val Lys Glu 100 105 110 Gly Cys Glu Ile Gly Phe IlePro Ser Pro Val Lys Leu Glu Asn Met 115 120 125 Arg Leu Gln His Glu GlnArg Ala Lys Gln Gly Lys Phe Tyr Ser Ser 130 135 140 Lys Ser Gly Gly AsnSer Ser Ser Ser Leu Gly Asp Ile Val Pro Ser 145 150 155 160 Ser Arg LysSer Thr Pro Pro Ser Ser Ala Lys Gln Lys Gln Lys Ser 165 170 175 Thr GluHis Thr Pro Pro Tyr Asp Val Val Pro Ser Met Arg Pro Val 180 185 190 ValLeu Val Gly Pro Ser Leu Lys Gly Tyr Glu Val Thr Asp Met Met 195 200 205Gln Lys Ala Leu Phe Asp Phe Leu Lys His Arg Phe Glu Gly Arg Ile 210 215220 Ser Ile Thr Arg Val Thr Ala Asp Ile Ser Leu Ala Lys Arg Ser Val 225230 235 240 Leu Asn Asn Pro Ser Lys His Ala Ile Ile Glu Arg Ser Asn ThrArg 245 250 255 Ser Ser Leu Ala Glu Val Gln Ser Glu Ile Glu Arg Ile PheGlu Leu 260 265 270 Ala Arg Thr Leu Gln Leu Val Val Leu Asp Ala Asp ThrIle Asn His 275 280 285 Pro Ala Gln Leu Ser Lys Thr Ser Leu Ala Pro IleIle Val Tyr Val 290 295 300 Lys Ile Ser Ser Pro Lys Val Leu Gln Arg LeuIle Lys Ser Arg Gly 305 310 315 320 Lys Ser Gln Ala Lys His Leu Asn ValGln Met Val Ala Ala Asp Lys 325 330 335 Leu Ala Gln Cys Pro Pro Glu LeuPhe Asp Val Ile Leu Asp Glu Asn 340 345 350 Gln Leu Glu Asp Ala Cys GluHis Leu Ala Asp Tyr Leu Glu Ala Tyr 355 360 365 Trp Lys Ala Thr His ProPro Ser Ser Ser Leu Pro Asn Pro Leu Leu 370 375 380 Ser Arg Thr Leu AlaThr Ser Ser Leu Pro Leu Ser Pro Thr Leu Ala 385 390 395 400 Ser Asn SerGln Gly Ser Gln Gly Asp Gln Arg Thr Asp Arg Ser Ala 405 410 415 Pro IleArg Ser Ala Ser Gln Ala Glu Glu Glu Pro Ser Val Glu Pro 420 425 430 ValLys Lys Ser Gln His Arg Ser Ser Ser Ser Ala Pro His His Asn 435 440 445His Arg Ser Gly Thr Ser Arg Gly Leu Ser Arg Gln Glu Thr Phe Asp 450 455460 Ser Glu Thr Gln Glu Ser Arg Asp Ser Ala Tyr Val Glu Pro Lys Glu 465470 475 480 Asp Tyr Ser His Asp His Val Asp His Tyr Ala Ser His Arg AspHis 485 490 495 Asn His Arg Asp Glu Thr His Gly Ser Ser Asp His Arg HisArg Glu 500 505 510 Ser Arg His Arg Ser Arg Asp Val Asp Arg Glu Gln AspHis Asn Glu 515 520 525 Cys Asn Lys Gln Arg Ser Arg His Lys Ser Lys AspArg Tyr Cys Glu 530 535 540 Lys Asp Gly Glu Val Ile Ser Lys Lys Arg AsnGlu Ala Gly Glu Gly 545 550 555 560 Asn Arg Asp Val Tyr Ile Arg Gln 5653 203654 DNA HUMAN misc_feature (1)...(203654) n = A,T,C or G 3gctgggacca caggcacatg ccaccatgcc tagctaattt ttaaattttt ttgcagagac 60ggggtcttcc tttgttgccc aggctggttt caaactcctg ggctcaaatg atcctcccac 120ctcggcttcc caaagtgctg gaattatagg cgtgagctac catgcccagc ctgtttcccg 180ttttttacta cagcccctcg gtgtagctag ggacatttca ttcatgcacc tccctgtgta 240ctcatgtgag ggtatccctg ggtatgtact gaggggtggg atgccaagcc tgagtacttg 300tgcatcaaca gtttcattat atcctgctaa atatctaatg tgactgtacc agtttatact 360cccaccaaca ggctgggcac agtggctcat gcatgtaatc ccagcacttt gggaggccaa 420ggcaggcaga tcgcttgagg tcaggagttc gagactagcc tggccaatat gctgaaaccc 480cgtaactact aaaaatacaa aaattagcca gctgtgatgg cactggccta taatcccagc 540tactcaggag gctgaggcag gagaatggct tgaacctgag aggcagaggt tgcagtgagc 600cgagatcgcg ccactgcact acagcccggg caacacagca agactccatc tctaaataaa 660taactaagat attctcccac caacagtatg tgaaagtcta gttgctctgc atcacaggct 720tacaaacctt ttctgtaaag ggccacgtag gacatattta aggctttgtg agttacatgg 780tctctgttgc aatgactgct cttgtaaaga gcacaaaagc agccatggag catacgtaaa 840tgaacaagcc cgtatgttcc aataaacatt tatttaagaa cattgatttg aatttcaggt 900aattgtcatg tgccacaaaa taccattctg ttttttattt tttccccagt aatttaaaaa 960tgtaaaacac attcttagtt tgtaggttat acaaaacagg tggctgactg agcttgactg 1020tgggccatag cttgccatcc ccttcttgac attttcacca agatctgaga ttttcagatt 1080ttttaaaaat atttccacca tgatggatgc aaaatggtaa actgtgattt cagtttgtat 1140ttcctgatta gaaaggaagt cctgtgtatt gaccactaga attttatctc ctatgattta 1200tctattcacg tcttgttttc catgttcttt tagttgtggg tcttttggtt gtgagccttt 1260tgatttatag gagttctatt tcctggatag taatacctat atatatacat atatgtgtgt 1320gttgcaagca tgctctcaga atattgtgtt tattatttca atgtttaatg ctgtctttta 1380atataaagaa gtttttaatt tacaaaaggt aaatgtctcc aagtgatact catttgactg 1440tgaaaaaaat tagattttcc atacacactg agaacttgaa aggcaacgaa aaaagaaaga 1500tatcactttc ttgtgtgcaa aagagaaaat atttcttcag tgggtgggag tagaaaaata 1560tttttacttt tattgcagac tttcaagaca gtagtttctt tgaatgctaa cagcttatcc 1620agttatgcaa cctgttgttc tgttctgaag aaaggattct cgagcagggc tggagtcctg 1680gctgcctgga gggacctgtc gcatctgata ctaatgaagt gttactgtac tttgacaagt 1740ttgcaggcgt ctgtcttcca agccagctga cgtaattcag aggagaataa gaccctccca 1800cttgcgtttg ttaattgact ttgatgagct cctctgctct gcctaagtgg ttctggaaca 1860gagagctgtt gtgatccgac gaactttaga aagtcacact aactaagact acacacccca 1920agccagcaag aaaaaggaaa gcagttgcta tgctgttcag caaagcaaga cttggctgcc 1980ttttagctag tcctgaattc ttgctcctgt gaagaaaatt cctgctggag tgctgggcgc 2040acttggaatt ggtctagcat gcttgacaga cgccttatag ctcctcaaac taaatacatt 2100attcctgggg taagcatacg ggagagaagc cggccagatg cacggtgcgg tttaaagaaa 2160acaggaatgc atagaactgt tgccgagctt gagctaaaat catactagtt acttaacttt 2220ttagactttc ttatcttgcc agaaatgttg ccattcacag tgttattact ttaatgctgt 2280ggtgatttaa gcaagtgaaa ctgtaactct ggttttaaac tgtatgttac tgagcttcat 2340ttagtagctt agggagagag tcccggtgca gacaaattta gtggaaaatg aaaatgcttt 2400ttccagctat agaccagcac ttgtcttgag tatcactctg acagcctagc cactgacttc 2460ttgtgtgtct gagacctatc tgtgtaaatc aggatggtct gacttgctgt gttgctgcaa 2520attaagattc ttttactatt catcaacttt agaaattttg aatttaataa aatacaaaca 2580tgacagtcta aagtttaaaa tacttaaaca taaagctttg ccacacgagg actgactgtt 2640tgggtcttcg gatttggttt agaactttta aagaaaagac tttattatta tttattggtg 2700cctttttagg aaaggatgaa aacatgcaca aaaaaaaccc tgaacctaaa actaaaccag 2760agcccgtaaa aataaaagtc ataaagttac aaatatctgt gctaatagga catgaaaata 2820ccattttaaa attaatagtt tgtctaaaaa ttattttaag tacttctttt gtttctaatt 2880gttccaacct atactaaatt tagtataata gagaaaaaaa tgcttgctag gaaatatctt 2940tgtggtcatt tcttctatgt tgtatcatat aaaactggaa actgagatac tttttatgtg 3000attttgatga ttcttttatt agattttact ttaagttttc taaagataca gaattttact 3060tgctgagttg gccctcatta agtattgtac gtgacaattc tatttcttta ggttctgaca 3120tggctggcaa tgtatacact aattatcaac ggtttctggg cattagatat gtcacgccca 3180cctatcaaat gctactcatg tccaatttag aaattattct gtacctgttt gagtacatct 3240ggaatcattt cctgtaaact tgcttgcttt ttaagaatat tatcaaacat gaaataactg 3300ctatattttt tcctgcagcg gaattctgtg aattacgaaa acatgtataa cctaaaatgc 3360aaattgccaa atacattcca ttggccattt tgaatatttt cagcaacaaa actggtcaca 3420attatgtcac agttatttaa taagaatgac aatctatatg atattaaata gtcacgaggc 3480caggtatggt ggctcacacc tgtaatccca aaagtttggg aggccaaggt gggaggatcg 3540cttgaggcca gaagctagag accagcctgg gcagcatagc aagatctgtg tctacagaaa 3600taatagttat cacatgaata atttatccta caattaattt tagtcctatc tttaagtatc 3660atggggaatc tgcacaggga tgtttagtct tttaaattac ttctttccta aaaatagact 3720ttttgtttcc tatggcctgg ctacctctca ttttaactaa taagtatgct tgaatagggc 3780ttataaacac atggattttt ggatttttga atggttaaag tttaaaacaa ctctttttaa 3840agtctttata accattcaac actttaaatt cagtctttct caaagttctc caataatgac 3900agaaattctt tcaagtttgg acaagaatat aaatggagat aattttttaa attatacata 3960tgtatattta tatatgtata tttatatgtg cctgtatatg tattatggga tagttgttta 4020gaatgctgaa taaactgtgt ggtctttatt tttgttctaa tcgcaagtat gatttcagtt 4080tttatatcag tttcagttta tttcagtacc ttctaaagtc attccaaacc tcaaatgcag 4140gaaacacatt tagaaattac ccatttaatt ctcattgtgc attaaaatga aatcaataaa 4200ccaatgcaat ttggtgtaat tactttctac cgccaaaggc aaatatatat agatcagtct 4260aactagtttt aagtgcataa tttatttatg aatcctggta tcattgcatt gtaaatttct 4320tgaacatggt atctttaata actgcaattg agtgccatta ttcttagacc cagatggtca 4380cttcctctct atcctttctt cccttcctcc aaataacaat gatctctctg atttgggcag 4440gttttttttt attattattg ttacccgtaa tttttgtttt ttctgactgt ggttaagaga 4500aagtatttgg atccagtcag acttgacgtt gaatactaaa ccgtccccta aataatggtg 4560tgatttggca gattttatct tctctgagcc tcagtttcat catctgtaaa attaggaata 4620ataataactg tgtttcaaga tacttgtgag aattttttaa aacgatcaga gtacatgcat 4680agaatataat tatatggatg gcttagcata gttcctagag ttattcaggg aaggtgtatg 4740ccttcctata ttttctgcaa tagctaggaa tgaaagaact tagctctgaa atcccaaact 4800gtgatacttc tgataggcag caaaggaaag tgatgagttg agtttttatt tgcatattgg 4860aggacaggga gagagattaa atgtccccct tgtgaagtgt gacttctaat tggagacctg 4920tgggtcagct atcagcgggc tgggagggga aagcaaatac aaacgctcta aaactgtgtg 4980caaatatttc agtctctgca tatgtgaaca ttttctagct tctgccaatt atcaaagtgg 5040tcctcaactt ctaaatggta ataaataaat aaatctgagt gggcgtaaac ctgagacaac 5100aaataagatg atgatgatga agtattctaa gaaggctagt aattttgggt cgattatttt 5160ttcttcctct ttaattttat attgctcaag catttatcaa aaaaaaaaaa aaaaagaaag 5220ttagcatgtg actgtgtatt cctgtatgtt ataagacaaa taatgatcta aggctgcagg 5280cttctacatc actcttcaga tataatttgt atttaaatgt aagtataatg tccataaggc 5340ctggtggcta atgtatgtac atggagatgc tggcttcagc atcaagagga gccatcctgt 5400ttgattttac tttgccccat aaaacgatca gtttgtggaa agagtcattt tgtgcagtcg 5460caatagagat ttgcagttat tttcattatt aaaattgaca aaactttttt tttttttttt 5520aaacagagtc tcactctgtc acccaggctg gagggcagtg acatgatctt ggctcactga 5580aacctccacc ttttggttaa agtgtttctc ctgcctcagc ctcccaagta gctaggatta 5640caggcgtcct ccaccaaccc cagctaattt tttttgtatt tttagtagag acggggtttc 5700accatgttgg tcaggctggt ctcgaactcc tgacctcagg tgatccaccc acctcggcct 5760cccaaagtgc tggaattaca ggtgtgaaaa gagctctatc cttcaaagta attaaagttt 5820ttactactaa aaatagaaga aattggctgg gggcagtggc tcatgcctga acgacaccag 5880cctgggcaac aactccgtct caaaataaaa taataaaata aaataaaaca gaagaaatat 5940taaaggaatg tgggtgtagg aataccccac aagctcacca tgctgtggtc agatattttt 6000atgctggcat attgtactgc agttcttttc tacaagcata catattttta tagttgtaac 6060tgcagtgttc agacagttgt gggttctgcc ttttttcctt caaccttatt ttgagatcac 6120tgtttaatca gatcactgtt taatcagcat aatataacat cgtgacgatc tgccattgat 6180tgtccaataa aatggtcatt tatttttgga ctttttaatt gattttataa ttttgttttc 6240atagataaat ctataacaaa tttgaacata tggttttcag ctccattaaa taacttattt 6300gagataaatt atcagatgtg gaattatttg cacagtgagt gggtttgaat gttcttataa 6360gtccctggct atgcttttga tatctttttt tccaaattct accagtggac aatgccagta 6420ttattcatat actaatttta tcacaatgta tgcagtatta tgtattaatg attgcttttc 6480atgtaaatag ttatacagca ttgttttaat ttctgtttag tggattacta ctgaaggtga 6540atatttatat actctctgta tttctgtgcg aattgtttca ttaggcttcc tgcatttaga 6600ttaataagtt actttttatt tgttggatat taacaggctt tccctctctc accttggaac 6660tcccatccgt ctgtctctgt atgggggagc ttcttccttc tttctttcct attaaacaca 6720ctccgctccc taaaacaaaa caaaaacaat ccttacagaa ttaactgtgg aatagttttt 6780cccctatcca atactatgct atcaaaatac tgtctttcat ataaatttat cttaaattta 6840tattctaata ccctactggt atctctagag gttggcttga agaaatagag tattcgcata 6900attagaaatc aacagataga ctagtcacag gattaaacct cagtttcgga atgtggagca 6960aaaagctttt gactttattc agatatctca cttatttttg acagggagct aataaccatt 7020acggagaata aggaaaaggt cacatctgac cgaagcacgc ttagtatgaa aagtgcattc 7080tgtctcacct ttctgttcat agattttaca ttgtcagggg cagcacaagc caacatatca 7140tcacatttct gttgtttcag ggctggcagg gcctggcaga gaccctgtgc ctttgtagct 7200tcctgcaggg ctccccctcc agtggccatt cagagatctt ccactctcct cttaagagtt 7260ggagaactgg aactttcttt tatttgtttg aggtggagtc tcactctgtt tcccaggctg 7320gagtgcagtg gcgcgatctc agctcactgc aacgtctgcc tcccgggttc aaacgattct 7380cctgcctcag cctcttgagt agctgggatt acaggtgccc gccaccacgc ctggctaatt 7440tttgtatgtt tagtagagac gggctttcac catgttggcc aggctggtct caaactcctg 7500acctcgagtt ccactcgcct cggcctccca aagtgctggg attacgggcg tgagccactg 7560cacccggcca gaactggaac tttctatatg gttttctggt aagggatctg ctagtatttt 7620gatcttaatg taatatcatg tcatttgtta attcacaaat agtcatcact tttccttcaa 7680cctttgttca gttacctagc tttgttaatg ttagtggctt ttttggggga ggaggggagc 7740tcggggggca cagggtctca ctctgtcgcc caggctggag tgcagcgata tgatctcagc 7800ttactgcaac ctccgcctcc caggctcaag ccatcatccc atctcagcct cccgagtagc 7860tgggactaca ggcatgtgcc accgcgcctg gctaattttt gtgtttttcg tagagacaag 7920gtttcacttt gttacccagg ctggtctgaa actcctgagc tcaagtgatc caccctcctt 7980ggcctcccaa agtgctggga ttacaggtgt gagccactgc acctggcctg ttagtggctt 8040tttggacatc agagaagaga aaaacctgac ccgtgaaatc acatctaaga tccttaattg 8100tgctgccaac acacaaaata gacctgctgc tgtcagtcag aaggggccat actcattgat 8160aaaagtgcac cttttagaaa gtctgtttca tactgttctg tgccgcctag tcttcttaat 8220agaagaaaaa ggctttgatt tgaaaacatg ataggatgga catatctgac tgcactgaaa 8280ggacaagaac aagattttat tttattttat tttatttttg agacggagtt tctctctgtt 8340acccaggctg gcaggctgga gtgcagtggt gagatcttgg ctcaatgcaa cctccgcctc 8400ccagatgtaa gcaattctcc tgcctcagcc tcctgagtag ctgggactac aggcgcatgc 8460cgccatgccc gtctaatttt tttttttttt ttgtatttta gtagagacca agttgcccag 8520gcttgtcacg aactcctgag ctcaggcaat ccgcccacct tggcctctca aagtgctggg 8580attacaggct tgagccacca tgcccagcca agattttatt cttaattcta gcactggctc 8640cttagttttt gagcaagttg attaacatct ctaggctttt tttttccccc tatacaatga 8700aggcaataat ttttatctct ctactgctct gtctggaagg ctataaaagc ctatacgggt 8760aatgcagtat ccttaaagca tctgagaata gctgtcccta gttttgtctt tactttgaag 8820cctgagtaat tacttaagga atgtaccttg aatcttgaga ccatgtttct aacaacacag 8880gtatttctgc aacctgaaat ctgatgagcc tgagatcctt tctgttggac ccaggttgag 8940gactattttc gtgacttcgc ttaatgagat ttaatgttca atataaacac ttgaaaggaa 9000tttttcaagg ctaaattcta catcagaagg ttttgagcgt cgtgcacagc tcaacttcca 9060agtcttttac agtgggctgt gaataattaa tgctgatttg atatctgggc attcttttaa 9120aaaacgtgta tatccaccca aatataacaa tatagaaaaa agaagagcct catgatattg 9180cccaaaattt taatgactca catgtgatat gctgagtact atctaggtga agttaaatgt 9240ggcctctgtt gacgaaattg ccacttactt ggataacata acaagataaa aacacgtttg 9300caacgatgta cattttgaga aatcatgaat ttctgcattg tagacataaa tatttaaaga 9360actctccggg taaaagatca gttgaaaatt tggaaattgt ggaattcagt tcaatttaga 9420atacttactg aatgacaaca attagagaat aattttgtca aatgtctctt taaaatgatt 9480taattaagat acatatgttt ggcactgtag ctggaataca gaggcccaag gtggttatat 9540gggctaaatt tgaactcaga aaaaggatgg atggacggac ggatggacag atggatggac 9600agacagacag acagatagat ggatggatag gtggatagat agattgtttg agacagagtc 9660ttgctctgtc acccaggctg gagtgcagtg gcctgatctc ggctcactgc aacctccacc 9720tcctgattct cctgcctcag cctcccgagt atctgggact acaggtgtgc accaccatgc 9780ccggctattt ttgtattttt agtagagatg gtgtttcacc agtttggcca ggctggtctg 9840gaattcctga cctcaggtga tccacccgcc ttggcctctc aaaatgctga gattacaggt 9900ttgagccact gtgcccagat acaaagttta tattttaaag agacctcaga actaggccag 9960gcaagatggc tgatgcctgt aatcctagca ctttaggagg ccaaggtggg aagatcgttt 10020gagctcggga gtttgagacc agcctgggca acgtagggag accctgtctc tacaaatata 10080ttttaaaaat cagccacatg tggtggtgtg tgcctgtgat cccagctcct tgggaggctg 10140aggtgagaga tcacttgact cccggaagtt gaggctgcag tgagctgtga tcgtgcttct 10200gcactccagc ccgagtgaca gaatgagtcc caccaaacag aacaaaggga cctcagcctt 10260tcttcacttc tttgttcttc atatgaaggg tgggcattca caggacattg caggaggtgt 10320gataagattc agagttcccc atacagcctc cacttgaccg cttctatttg aaggatgtga 10380tgctgtggct ggaacaactg gttggtgaaa atgttgttga aagtcgtttg tttaaagaga 10440cctaaaaaga accgttttcc cggagctccg gcatactaca atgacctaag ttaatagacc 10500acagcgagtg tgaagaataa tgggtccaat tgttgatgca gtatctgctt ttaggctgag 10560aaaatatata ttggtttaat ttccacaact gagtttgcct gacttctctg ggaccatttt 10620ccattccagc agcataaacc tggctcaaaa tgccattaaa attgatgaca atctgctagg 10680tgagatgagc cagacacaag gagacaaata cagaatgacc ccacttatat aagggaccta 10740gaatagactc acagagtcta gaatggtggt ttccaggggc tgggagaaga ggggagtgga 10800gagttagtgt ctaatgggga caaactttca gttggagatg atttaaaaaa aaaaaaaaga 10860tcctggagac agaagatggt gatggctacc caataatgtg aatgtattca atgccattga 10920actgtatgct taaaattggc tcaaatggta aatcctatgt tatgcatatt ttgtcacaat 10980gcttaaaata caatttaata aaaacccacc tgtattgcgc acgtatgttg catgaggcca 11040aatgctttat atctattatt attattttta attccgtaac agctgggtgc ggtggctcat 11100gcctgtaatc ccagcacttt tggaggacga ggtgggtgga tcacctgagg tcaggagttt 11160gagaccagcc tggccaacat ggtgaaaccc tgtctctact aaaaatacaa aaaattagcc 11220aggcatggtg gcgagtgcct gtaatcccag ctactcagga gggtgaggca ggagaatcgc 11280ttgaacccag gagatggagg ttgcagtgag ccgagattgt gccattgcac tccagcctgg 11340gcaacaagta gcaaaactca gtctcaaaaa aattttttta aaataataaa aataataata 11400ataattctca taacaaccca gtgaagtaga cacatgatca tctctagttg gcagataaag 11460catctcagca cagtcaagtt tgtcactagc gtggggtccc acaggtgtcc gtggaaaaag 11520caggaattgg aagcaaaagg gcagcctctt ccatgcacgc agcccacaca gcactgcctg 11580tgcctccttg ttgtttttgt ttgttttgcc tctggtgact gcactttcta atggccccag 11640ctgctagagc agtcgacgtt ccctcaggag tagcgcttta ggttccctta atcttcaccc 11700aggggtacct ccttgacctt gaaccctgca ggtcctgtag caggaggtga gattgcccat 11760cagccctcca ggagtccacc agacctggca ggccttcatg cagcagaaca aaaggccagc 11820tgtttatttc ctaggcagag ttcatgaaaa tcaccccatg ggtgtgatac caagatcatc 11880ttaaaattaa cttgtgtttt aaaaaccatt ttctgtttgg ttttttagtg gtggtggttg 11940tttttgagac agggtctcgt tctgccgtcc atgctggagt gcagtggcac aatcatagtt 12000cactgtggcc ttgtactcct gagctcaagc cgtcatctat cacagttaaa aaccattttt 12060aatcttctct aaacaagtaa cttttcttaa catagttttc aatcttctag aatgatcaac 12120tagaattttt ttaaatgagc tgcttttttc ttattatatg ccagctactt tttggaaaaa 12180caatagcata gagtcattct cacaggagaa tgtatataaa aacaaagtaa atatattttt 12240ataaagatca catgtacacc ctttcttttc tttttttttt ttcttttttg ctaaaatgtg 12300tatttataac cagatttctc atcaggcttt cttgggatct ttaaaatatg tttcttttgc 12360aaatataggc ttaggactaa atatattctg tatcccgtca tcatgttgta ttacagatga 12420gaagacaatt aattctgtga taaagtatca taaggcaaga aagaaggtgc ttggggaaat 12480tggactgtgc tagagatctt ttctgattct gcaatatact acgtattcag tgatctgcta 12540tatacaatga ttactgtctc tttacattct tctacctata ttttttaatt gggtgagatg 12600atgaactgga aaatgatttg ctacatccag aaatttcaaa gcattcttaa ggattttgtt 12660gaaaacacat gttattctat tgttaccgtt ttctgccttt ttcactctac catggaaaac 12720cagatggtct gtacaagccg agagaaataa tttccaaccc agtgtatttg cataatttcc 12780tctctgccct taatctggaa caaaaggaaa caaaatgaat tgagttcagc ataataaact 12840agattggttc ttaattacag aattaggaaa gtttttcaca taatttaaga ttctttggtt 12900gtattgcttt caaagttaga gaaagtctct tttaaagtgt gatttctatt agaggctttt 12960tttcctgatg aattcattga ttcaacaagt caaaatgggt gacagttttt gcaaaccaaa 13020ggaagagagt gatgacattt aaaacacata tatagacata gagaatttca aatgttctac 13080actcctgctg ctctctcaag gcgtgttcag agtctcctaa aaaggatgtt tgacctgttt 13140gcagagctct gacagatgat attttgacag gtctatttct tctgcgtgtt caatgcaatg 13200cccaagatga ataggcattc aacctaaaag tacaacttaa aagtacattt ttgcatccaa 13260gccagcctct gcgtacttca ttcttatcct tatataaggg taagatattt gatattctga 13320taatcctgtt acctttctga tcgcatgagt tgagtggact tccacgcatc acgtcccatg 13380acagggctcc tctgtgaagc agggaaaaag catggcatct gatctcctta attcttacaa 13440caccaacaga acataattac tttatcatac agggctatga agatagcaca tgtcaccact 13500gtatgaaagc tcttaggttg tggaatttag tctaatgtta tcatggaaga gtactgaaat 13560agggaacagt agggttgata aatagagaaa gaaaagagaa accaggaata atcagtgaat 13620gataagcaag taacatggtt taaaagcatt ccaggagttc catttttaag cactcttgtt 13680tacttagttt tgctacccac ttgttgcttt ggaagagttt ctattaatta gatgtcattt 13740ttcaaaagtg cgtacttaat tgttatatga attacaaggg actgcatagg tttcgttgca 13800aaaatgtttt tcaaggaaca cttagaggaa acatgcttcg tagcatttca tagtatatga 13860catttttaag ggagaatgct ttacatgtaa tatgtagcac aatttggaat tattagtata 13920ttggattttc tcatgttctg ggcaaatgaa ggaacatata tgtttgtatc aataaaagct 13980gtgaacagaa tatgtgagca tattttgaat ccatcaaatg tattacatca tctctagtaa 14040agctataaat tatagaatac tttaataaag ttatttttct tattcatgaa atattatgtc 14100tggtagcagg aaaatgtgat ctgttcagaa aaaggataat gaaagaaatc ttttattaga 14160gaaatatgat tgctatacaa taatgtggcc tacatttaat tctacatgtt ttaaaattag 14220aattgggctt taaaaattta tggttctatg ccaggtgagg tggctcacgc ctgtaatccc 14280agcactttgg gaggccgagg caggtggatc acaaggtcag gagttcgaga ccaccctggc 14340caagatggtg aaaccccata tctactaaaa atacaaaaat tagccgggca cagtggtggg 14400cacctgtaat cccagctact ctggaggctg aggcaggaga atcgcttgaa cctgggaggc 14460agagtttgca gtgagctgag attgtgctgc tgcactctag cctgggcaac agagcaagac 14520tctgtctcca aaaaaaaaaa aattatgatt ctatatgaaa cgtacgtttg tatggacttg 14580gaaaagtctt agttaaggac ataggccttc tcaactcttt ttaactctgt aactttatga 14640tataaacaaa atatgtaagc atttcttcac agttcgccga tctacgttga ttcagtataa 14700ggtacacatc aaagtaaaat taaccaaaga ctctaatttg agggaataat aaggcacatg 14760gttgataaga aagaatacaa tattccaggg gttatgaact attagcattt ctgattcatg 14820gcaatccaat ttgagagtat aaatttggat taccagctta gttctgaaaa actttcttta 14880tactgctcta tggcagctga gggcttccgg ttcatcacat gtatctgttt agtctgctaa 14940ctctgacaaa agccattatc tatttttttt gcccaaaggc attataagga aatagtatga 15000gcaaagtttt ctttctagta taaacaataa atcttactag ccattgggca aactcatatt 15060ctggtgaata tttgaaaaag cttggcactc attaatacca gcaagtgtcc agtagcttga 15120tgagtacttt atagtattta aatggaatgt atagtctgtc aaaaaactat agtaggcctt 15180aattagctgg gtgacattgg acaaattatc cagagtcaaa tccctgagag tcaatttgct 15240tatcttttca tttggagtaa taatgatgta tgacccctat agacctcaag gctcaaatat 15300aatttgaaaa tcttaaaaat agcaaagcac tgtatgataa tgaggtatta atcatttaac 15360aaacacttgt taagtgttag ctcctttttt tcctcctgac ttgggccctt aaactgcttg 15420cctgtcttgg gaagagtctc cttggtgagg tcctatgaga actttccact gtgcctggtc 15480ccaaccacca cccaactctc cacccttcca ccaacttgtg ctggccttga aaaggtcaga 15540aggctgttgg aagagtcctg gggagagaaa catggcagcc gagcgcaaga cagttatggt 15600ggggcctaga gaggagccag atgtcatggt gtggttgatt gtgttggatg aagctgaggt 15660cacagaagat gaaggctgaa aggaaagcta gggttatttt gcggtgtttt tgttgttgtt 15720gttgctgttt ttgcttgtat gtttgtttct caattacaac tatagggatg ggggaactat 15780ctttagagga gtctcaggac ccagcaggcc caacaacaga ggagtgggga cttctgagaa 15840gtctggcaga gcaggtgatg ggcagtagct tttccggttc tgaaaatatc tgcataatct 15900acacaggggt cagcagggtt cagagaatta acctaaagca caaccataaa accatgtgtg 15960gtttctttct tcaaagtcac atctgaagct caatattaca ggttgagcat tccaaatcca 16020aaaatccgaa atccaaaact ttttgagtac ttaacgtagg ctagagacac ctttgcttcc 16080tgatggttca acgtacacaa actttgtttc atgcacaaaa ttatttaaaa tatcatgtaa 16140attaccttga ggctatatgt ataaagtgta catgaaacat taatgaatgt tgtatttaga 16200cttgagttcc atccccaatg tagctcatta catacatgca aatattccga aatccaaata 16260aaatttgaaa tctgaaacac ttctgtccca ggcatttcag gtaagggata ctctgcatgt 16320acactctaag tgtttcaggg tgcttaacat agtacttcta tcatttcttg ttatttgagc 16380tttctagatt ttggagttct ggatgagaag cttatctttt acaaacatta cttgtagtta 16440ttcttttcca ggtataaacc taggtagaaa aaagttgtta taatgaaaag aaaaatgtat 16500cgtcgttttt ttaaaaatgg gacttctata tgtaactaac gatttggtgt tagtgtgagc 16560tatgatttgt tctcaggatc atgaatgata tcacacaaat gattacgaca cttgaataaa 16620tgtggccttt atttaatttt ttgatgctat tgcagagctg ctgctaacat tcagtcctag 16680ttataatcac aaaagtatct gccataagcc cagctgatta ggctctgcaa gtttgatctg 16740atttttctct tttttttttt tttttatttt tttgagacag agtcttgctc tgtcgcccag 16800gctggagtac agtggcgcga tctcggctcg ctgcaacatt tgcctcctgg gttcaagcaa 16860ttctctgcct cagcctcccg agtagctggg attacaggtg cctgccacca cgcctgcctc 16920atttttttgt atttttagtg gagacaggat ttcaccatct tgaccagact ggtcttgaac 16980tcctggcctc atgatccacc cgcctctgcc tcccaaaaca ctgggattac aggcgtgagc 17040caccatgccc ggcctctgat tttttattct cctctgtaat gtccgagtca actaacatgc 17100acgtaaaata tgcaaaaaac cccacaactt tctggaaggt ttctgtaagg attgctccac 17160ggagactctg gcaccccaag gctgcaggcc cctggaggtc ccaggagacc tggatctctg 17220cccactgccc catgcattga cacagccctc ccttcccacc ttccccgctg taatattgct 17280tacccatctt gattccttca aggatgaagc tgccataggc tgggcctctg ctcctgcttt 17340tttctgatgc ttcattcatg caactatccc aactgcccag cacccacttt gcatcacgct 17400agctctgggc accatcagga agcccactgt ctataccctg tgnnnnnnnn nnnnnnnnnn 17460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctccctg ccctttactt tgatcctaaa 17520catctctgtg gttctgcaga aaagcttttc ctgactacat tgcacaaagg agcttctccc 17580attattcaca tcacccagtt tgcttcgtat aaatgtaatt atttgaccag cagtttacat 17640tttgatcctc tctcctctcg tctctttttc tccttcattt ttttttttaa cagagtctta 17700ctctattgcc caagctagag tgcactgaca tgattgcagc tcactgcagc cttgacctcg 17760agggctcaag caatcctccc atctcagcct ccaagtagct ggaaccacag gcacatgcca 17820ccatgcctgg ctaatttttt tatattttgt aggggtgagg tttccccatg ttgcccaggc 17880tggtctcgaa ctcctgggct caaacgatcc tcctgcctca gcttcccaaa gtgctgggat 17940tacaggcttg agccactgtg cctggcccag actcaatgtc tttttttttt tttttttttt 18000ttttttgaga cggagtttcg ctctgtcgcc caggctggag tgcagtggcg cgatctcgac 18060tcactgcaag ctccgcctcc cgggttcacg ccattctcct gcctcagcct cccgtgtagc 18120tgggactaca ggcgcgcacc accatgcccg gctaattttt gtatttttag tagagacggg 18180gtttcaccgt gttagccagg atggtctcga tctcctgacc tcgtgatccg cccgtctcgg 18240cctcccaaag tgctgggatt acaggcgtga gccaccgcgc ccggctgact caatttcttt 18300tacctgtatt tcagcactcg tcctttatca tcctgaagtg aaatgtatag ttaatgcaaa 18360caaccaacac acaaattttt aaaaaccatt tgggtcaaaa tactgaccca atgaataaaa 18420agattaattt attctctggt aatgtgtatt tcaatgggtt attatgctgg cataaccaca 18480ctagaaaacg tgaggaagca gttccatgga tagggccact gcatggacgg attagcggtg 18540atgtacacct acaggtgtgc ggctattaaa gacaatagtt aacaatttgg taaagttcca 18600aacaaagtac aatctttcat tgattgtcac agtactttca tttctggaaa attcagtata 18660ttttacaact atgcgaaaag acttgtactc atatccaaaa aaagatttag attctaggtt 18720cagataattt tatatttttc gcccacatga attccagcag gatagtcaaa gatcgtgaga 18780ggaggtgcac atccatctta taatcacata ggattgctgt gtaaaatgca aacaggtgta 18840ttcctagcca ctgtgtacaa gcatcctgta atgcttctca gtcattgaaa caaccaaaaa 18900tccagcagta cctttctgga acactccttt tggggaccca ttagtttaga cgataaactc 18960cttaagctcc tcctgcatgg cgggcaggaa tcatatttgc ctcatctact atacaagcct 19020caacatccag cacatcattg tcgcagtgca tggcaaatat ttaaagaagg aataaacgaa 19080tgagtgaatg gaaaggaata cgcagaaaca ggaaaacaca ttcatccatt caattgattg 19140attaactgga gacagagtct cactctgtca cccaagctag agggcagtgg cacgatctca 19200gctcactgca atgtctgttt cccaggttca agtgattctc ctgcctcagg ctcctgagga 19260gctggtacca cagacacgcg ctaccacgcc tggctatttt tttgtgtttt tagtagagat 19320gggttttcac catgttggcc aggctgttct caaactcctg gcctcaagtg atccacccac 19380ctcagactcc cagagtcctg ggattacagg tgtgagccac catgcctggc ccatctattc 19440aatgtataaa tgtttactga gcaactagta tatgctacct gctgttctag aacctaggaa 19500tacagcaatg aatgagggaa attccttttc taatgagcac gctctctctc tctctctctc 19560tctctctctc tctctctgga gctgctttgt gattgcagta tgacatctgg ttttcctata 19620caggtacaag gtggctgtgc ttgagacatg ccaagttttt tttaactcag aatacttacc 19680atataaatag agcaatagag tatgatagac aaagctggct aggaaataaa aagcagtgga 19740aggaattcag tttgatgaca aagaagtgtg aattgccctt aagaaacaaa caatagaata 19800aaatgagagc ctgaaaataa ataacttggt tatttataga tttcttgtgt ctgtcaatac 19860agcatcacag accaaaacat ttaaattcag gggttttgtt gttgctgctg ttcttttcat 19920aaatatggcc atattagaaa aactttacat ggcgtttttc tcttatgcaa tcttttttat 19980gtagcagttt cctgggaaat gttatgtcag ctttaataca ggcctgaatt taactggcaa 20040atagtgacac aaattgtcat gatggaaaag taacatgttg ggggttttaa taaccactga 20100aatactgata gccattttct ttttttttta acttctacgt tatttaggga cctattttgc 20160agacataacc ttaaactaaa atatcccgtg tccattgaga agcctacccc cacaaaaata 20220aataaataga tgtgggaatg atatttggag tttgtgagta atgtttgttt tgttttttgt 20280ttgtttgttt gttttgacac agggtctcct tctgtcaccc aggctagagt gcagtggcgc 20340catctcggct cactgcaacc tctgcctccc aggttcaagt gattctccca cctcagcctc 20400cagagtagct gggactacag gtgtgtgcca ccatgcccgg ctaatttttt tttattttta 20460gcagggatga gattttatca tgttggccag gctcgtcttg aattcctgac ctaaagtgat 20520ccacccacct tggcctccca aagtgttagg attaccagca tgaaccacca cacccagcct 20580ggagcttata agtaacattc tgttttgttt tttttttctt tttcatactt taagttctgg 20640ggtacacgtg cagaacatgc aggtttgttg cataggtata catatgccat ggtggcttgc 20700tgcacccatc aacctgtgat ctacattagg tatttctcct aatgctgtcc ctcctctagc 20760cccccacccc ccagcagtcc ccagtgtgag atgttcttaa actggcatgt ttcttggcca 20820catctcttga attttaatta ttgtactttg aattattttg atgttaagat taacttttat 20880gcttatgctt attgctgtcg tgagtggtac taaagagtcc tgggttgtaa attgagccac 20940gagtgcaaag aggaaattaa actattctat attcaaatta agccataaac ataggaactg 21000gactctatac tggggagggc gggtaagaac agtacaggag catagggtga tccagcgtca 21060cacagagcag gtgctcagga aagacatcaa taaactgccg atgaatcaac attgtaagaa 21120caggggaatg aactttgaag tttctttgac atcagttatt ccaacaacct cagaaatagg 21180tgctgcctcc tctcctatat aaaggaggaa actgaggcac aaagagttca gtttaggttt 21240attttaagag acagagtctc actctgttgc ctaggctgga gtgcagtaga gcaataatta 21300cagcttactg cagcctcaaa ctcctgggct caagcgatcc tcttgcctca gcctcctgag 21360tagctgggac tacatgtgtg caccactata cctggctaat tttgtttttt gtagggatgg 21420aatctcactt tgttgcccgg gctgatctta aatcctggct tcaagggatc ctcaggcctc 21480agcctcacaa agtgctggga ttacaggcat gcaccactgc acccagcctt attttgttgt 21540taaggcaata tttatgctgt gtttctaata attcacttat cactcacaag agtcctgtga 21600ggcagaccta ttgtttttat ttcacagaga agaaaactga ggcacagaac tttaagtggc 21660agctttgatc acggggctaa gatatggtgg ccacacatag tcatgagtga ggagagcagg 21720ctccataact tgtcctctta gacgacttgc tggctgtata ttcccctgca tctggggtgt 21780cagattatgc acctgctgaa tacacagcca gcaaggagca gaacaggact gtctgatttc 21840aaaatctatg tcctttcccc tatccttagc tgcaaaacca gtgatattcc ctactcactg 21900tgggaagaag catgaatgcc ttcctaatca tcaaagccaa gaggttttgt ccacaattca 21960aattcctgct gacctttctt ttctgttcat tgttcatccc tccttcatct cattgtggtt 22020tttttcctgc ctcctacttc tctgagcatg acctctcatt cttctctgac ttttccccca 22080tctactaaat gtgtgtgttc ccttaagttt tatcttaaac tctctttatt cttttatctg 22140cttggagcat ctgtgtctta gcattctcct ctacttcccg ggctcatcca ttacctttat 22200gtagccatcc taaagtagac ttttcaaaaa ttccacctgt aatcccagca actggggagg 22260ctgaggtgga aggatcgctt gagcccagga gtccaagacc agctggcaac ttaatgaagc 22320cctgtctcta caaaaaatta aaaaaaaaaa aaaaatagct gtgcatgatg gtgcatgctt 22380gaagtcccag caacttggga ggctgaggct ggagaatcgc ttgaatccag gaggtggagg 22440ttgcagtgag ccaagatcgc acccctgcac tccagcctgg gcaacaaagc aagactctat 22500ctcaaaaaaa aaaaaaagaa agaaagaaaa aacaattcca gtcatataag tccaattgct 22560tatcatacat ttctccacgg ttgttatatt actgttatcc tgtctaaggt aaaattcttt 22620tttttttttt ttttcgaaac atagtcttgc tctattgctc cggctggagt gcaagtggca 22680tgatctcagc tcactgaaac ttccaccttc cgggtcaagt gattctccca tctcaagcct 22740ccctgaatag cggggattac aggtgcatgc catcatgcct ggcaaatttt tgtattttta 22800gtagagatgg ggtttcacta cctaggccag gctggtcttg aactcctgac ctcaagcaat 22860ctgcccgcct ctacctccca aagtgctggg attacaggcg tgagccacca cacccagcct 22920aaggtagcat tcttaattgc tgtttcccaa aaccaacccc tgtttctaac ttccatatta 22980ttactgctac acattttttt atatttttta tattttgtag ggatgaggtt ttcccatgtc 23040gcccaggctg atctcaagct cctgggctca agcaatcctc ctgcctcagc ttcccaatgg 23100gtagcagata taagacaatt tgtttaacaa ttaacactca gtttatctag cagtggttca 23160cctaatgatt gaagcctctt tcgagtgttt ttgtatacat gcatacatcc tattcttttc 23220agccatattg tttttcttgt taaggacttc ttaaacatgc ttattaaaac attttcaact 23280taatttgtat ttctaaaaag tcatgatgaa ttttgtttca gttatagcta ttaagggttt 23340tctaaagatc ctctcttcaa aatgttttgt taggagcaat agatatttgt aaaatttatg 23400aaaatttgac tagttttttc taattcttta cattgtgtga ttactgtttc ctccttgttt 23460ttttccccca agtgattata ttagtattct atttttgtca ttttagagaa tggacacaga 23520agtcaccata ggtgaattga cttaaaatct tttaagtata agaaaaatag caaaaatgat 23580aacctgcgaa gtgggtgatc aattttttaa gtccataacg tgttaaaagt ccataacaaa 23640tattgccata gaaaatgcat gtagaggctg ggcgccgtgg ctcacgcctg taattccagc 23700acttcgggag gtcaggaatt tgagaccagc ctggccaaca tggtgaaaac ccgtctctac 23760caaaaatgta caaaaatggt ggcacatgcc tgtagtccca gctacttggg aggctgagga 23820gagagaatca cttgaacccg gcaggtggag gttgcagtga gctgagatcg ctgacactgc 23880actccagcgt gggcgacaga gtgaaactct gtttaaaaaa aaacagaaaa acaaaagaag 23940gaaaaggaaa tgcaagtaga atgttagacc aggtcttcat taagtgactt cattaactct 24000gggctaattg gctctacctg tgagcaaaag ctgattgtct gtgttgaaga cagtttatcc 24060acctgtctct cctctctgtc atagtcacat gttgcctctc ccccatcttc tctgctcaca 24120catctcagaa gctcctaacc agcttttctt cccatgaaca gtaaaatagg tttcctaaat 24180aaagattttg acatcccagc cccttgtgtt aaaatctggg atggctcatt atttacaaga 24240tcaatttcaa aagtattagc ctggcgttcc aggctctcaa caggatggat ggctcaggct 24300tggcttttca gacataacac ctatggtttc tgtacaagca ccaagcagca cgtctactca 24360cttgaggatg tcttgtaccc agtcaccccc gctttcttcc tggttgaaat cctgtcgccc 24420catggggcct tctctgttcc ccatggaagc ttcatcctct gatcctcagt gccatatggt 24480atttgttgtg ctggttattt cttgacatgt ctgtcttatg ctttcaaaga gtgtgcactc 24540tctgaaggtt cagcatgaaa ctaccagtac cttccattcc cttcagtctt ctattctagt 24600aagctcaaag ctagcagttc aaagctagta tttaatagca tgtttaaaat atgtatatga 24660ctagacaatg tggccaaagg aagtgtctga gccagagggt ctgaattcag gtctttgtct 24720aacatactca ttatagctgt gtgacacttg gtatggcacc taacctctct ggaaatcagt 24780tctttttttt ttttctttaa atagtaagga tagttatacc aatcaaatag gtttggttta 24840aaaacagatc attttggata agtgcattct atatcataaa ctgctttaaa aatgttaata 24900ctggcaatat ttttattaat gctgttgcga atagtagggt cttgaaggca gtttaatttt 24960tttttttttt ttttagatga tgttttgctc gttgcctagg ctggagtaca atggcacaat 25020ctaagctcac tgaaacctcc gcctcccggg ttcaagtgat tctccagcct tgcctcctga 25080gtagttggga ttacaggcgc ctgctaccac acccagttga ttttgtattt tcagtggaga 25140tggggttttg ccactttggc caggctggtc ttgaactcct gacctcaggt gatcaggtga 25200tccacccacc tcagcctctc aaagtgctgg gattacaggc atgagccgcg gtgcctgcct 25260tctacatgcg ttttctatag caatatttgt tatggatttt taactaattt tttttttttt 25320tctgggagat agagtcttgc tctgtcaccc aggctggagt gcagtggcaa gatctcggct 25380cattgtaact tccgtcttcc gggttctagc aattctccag cctcagcctc ctgagtagct 25440gggattacag gcacgcaaca ccacgcctgg ctaatttttg tggtttcaat agagatgggt 25500tttaccatgt tagccaggct ggtctcgaac tcctgacctc aagtgatcca cccaccttgg 25560cctcccaaag tgctgagatt acaggcatga gccacttcgc ccagccaagg cagtttaatt 25620tttttcttct ggtcttgtct tctctttctt tctacatttc tcaccctctt ctttcatctc 25680ctgcctgttc tacatctgtt acctctgacg tgcatttgtt gccaccgttt cttttgtgtg 25740tatcaattta ctttcctctc cttgctttag atatctttca tcttcattcc caatcttcct 25800ctttctgcca aaatgtcatt ttccatgcga tatttcttcc ttcattattc tttagcttac 25860tttcttgtta gtatgtataa tagggtttaa aagtgatcgg agattaaaga attatcataa 25920aatccagaag tccatacaat ttctcttgca cttaggatag catttttttc aagtgctgtt 25980ttatttgtag gcacagaatt ttggaatgtt agtagtagaa agaggtgtat aaattaatct 26040aatccaatct acttgggtta aggatgaaag cagatgtcta gagaagttaa ggcacttgtc 26100ttggacctca gagttaatta ctgactgagt tgatagttaa gaccatttcc tgagttcaac 26160ttactcttgt ctcatttttt gctaaccact gtattgtgtt tcctgcccct gagtttggtt 26220aagggcacca tctgggagtt agtttgtttt cagcatgcaa tcaagctgat tggctttgaa 26280cgagttattt acccctctgg ccttgacatt ctcatcagaa tatgagagga caatatagag 26340attctttaag gcctcttttt aattttcaac ctttcttcat ccatatttga cagttatttg 26400gtgacggcca attatttaca aataaaagcg atttggactc ttacattcag tttgccttca 26460tgttttattc caaatgccac ccaagtttgg gcttcttttg ggcattactg tattttaatc 26520tatacaacct cataagcttc tccaggattt gtttaaatta tttctaatta aaacatgatg 26580gctttctggg cagaaatcct gtccttgtac tacaaggatg tttggcaagg ttattgttat 26640tttttaagag gctgaaggaa aattaaatat tttgctgaaa tattttctct ggattataac 26700gttgggacag aatagtgcaa catgcttagt gtatactacc tgaatgttga aaaagagagc 26760ctccattatt ccttatttgg agaacagtat agtattaacc attcattgat ttcagtgaat 26820actttctcac acaaattatt cacaatgtga aggaaactca gatcaagaaa gaaaagaaat 26880gtatagaact atctttgttg aggatgtaag aatctgtcaa tttggcctgt ggagggtagg 26940aattccagga agtttctgag ccctctcact tctcacaaac actcacattc ccttcaaatt 27000catagaaaac cattctaccc atttaaataa acaagaaata agtcttgctt atttcattct 27060ggttcattag ggttcttttc ccatctacct ggctcactgg aatgttggct ggttctacct 27120ttcaaatgta ggagcctttc cacatctagg tctgggtcat gtaaataaac agaaacttga 27180atgaaattct cgctgagggc ggtggctcac gcctgtaatc ccagcacttt gggaggccga 27240ggcgggcgga tcacgaggtc aggaattcga gaccatcctg gctaacacgg tgaaaccccg 27300tctctactaa aaatacaaaa aattagccgg gcgtggtggc gggcacctgt aatcctagct 27360cctcaggagg ctgaggcagg agaatggtac gaacccggga ggcggagctt gcagtaagcc 27420aagatcgcgc cactgcactc cagcctgggc gacagagtga gactccgtct caaaaaaaaa 27480aaaaaaaaaa ttatcctggc ccagcacttt ctttggttta ttttgctttt aataatcaat 27540taatatagtc catcgaagac tgattttggg ctgggcagag tggctcacgc ctgtaatccc 27600agcactttgg gaggctgagg tgggtgaatc acttaaggtc aggagttcaa gaccagcctg 27660gccaacatgg tgaaaccccg tctctaaaaa tataaaaatt agccggaaat cacttgaact 27720cgggaggcag aggttgcagc gagcggacat tgtgccaccg cacactccag cctgggcaac 27780aaagcgagac tccgtctcaa aaaaaaaaag ctgattttgg ctgtttatgg aattttcagt 27840cagtttgcca gagcatcatt tcctgaagtg cttacaaata gtcatttttt gtcttttggt 27900gtctgagagc ctattttaag agcattgcca agcgtgggaa actagtatca ctatattcca 27960gctgtggaaa ctgtatcact gtcataatgc acttgaactt cctatgaaag gatttcttag 28020cggacatgct gagaatttac ttcgaggtgt gtacgaaaca tgtactacga tgacgatgag 28080ccttctgaag tggtaagaaa catataccca ttgacatctg acatttttca tcattttttt 28140aaccatttcc aagtttgttt ttaaactttc cactaaagtg cttgttgtca ctaacagtaa 28200tataaaacaa atagtatttt ttttttttcc cttagggact agcacaaatg ttgaaatact 28260ttgggacaaa gtttgaaatg aacacatttt ataattatat tattttgtta tatgcggaat 28320tattttttgt gcttgaactt ggattttaaa tagttttcaa tatcttcaca cgtcttctga 28380aaaggtattt ttaaaaggtt ttataagtgt aactatagac agtttacatt cttttttaaa 28440aaacttgatt tcctagtgaa attatatctg ttaaatatag ttaaaagtaa ttgaattgag 28500aattgtatat gttataaaac tttggatgat ttgtaaatac ttagtaaact gttagtgaaa 28560cctcactgta agaaatgttt ctcttaaatg tttaaaaaag tatttaaatt aagagtatat 28620aactaattag gattctgtat tgttctcaag acataaaatt atgtaaaaat tcacatttat 28680caatacacat agcaaaacag acagtaaatg tagtcacctg ttccttgtat gcaaataaaa 28740gatgaagcta aactttaaga gttagggata catttttgaa attgtagaaa gctatattaa 28800accatatact tctttgaaat agtatataat tcaattcaaa tttaataatt atgtttctgc 28860ttccttaata tcttattgaa ttgtgatgta atttaatttg attttagaaa gacttaagaa 28920ttctaagttt gactgttaag aaacgaacct atgttaaaga atatattttg gccgtctggt 28980catattctac tgaaaacgaa attccctttt taggtgcgtt tgggggtaca gtagaatgag 29040attatttcac tttttgtcat atggccagga ggaaagaaat ttcttgaaaa gcaactgagc 29100tctctcctgt aaacacagca agttgaaatc gcacagctcc taatttggtg tctttatttc 29160aatctttgga gtaagattat gaacaatagc tataattcca ttctgagagt taaaagtttt 29220cattgtaaat ttcaaaatgg tcatttctac taatatgggc attatttttc caaagaccca 29280cttctgtctc ttgtttttcg ccctcgggtc actggcaata tggaaaaaaa ctttcaaaag 29340agtaggatat gactccattc cagtgttccc aggaactgaa agtgattgca aaattataag 29400ctttcagaat aacacagaga aaacttctac gaaacaagta atgcagaaat gcatccaata 29460tttaggcctt gctctctatt tttattgcct gcatcacatg tggctcatac agaattttca 29520aactatttaa gagttgcatt taagcatatg ctaccaaaaa ggaaaaaagt attttgagga 29580atggtgttaa cattttaggg aaagcattcc tgaaaatgag ttccaggttt tgttattttt 29640ttaataaaag aatgtaaatg tatacagaaa ttatttccta gaatgtactt gaaggagtat 29700tttaggaata caaacattca ttaattaacc ctcatgtgtt taaaatatac acctgttata 29760tatgattatg cacaaaagcc agtttttcat gaataatttg tgtctttttt acataatttc 29820agaaaccata tgctctataa tgaagaaaaa atatttaaag tatgtaatca tgttaaacag 29880ttacagcatg agtcactcta tttaaaaaaa aagagagaga accagtctgc acgtgacaac 29940aagaacttaa aggatctggt gttcatttga aatgtactgg taatttaaca tcactgtaaa 30000ttcattaact taggtcattc tggttattga gatcaaattt gaaatttttc ttttgttaag 30060tattcaaatg tttaaaagcc ctgtaaacca gatcagagta ttaggacata aatttgagtg 30120tgtttattcc ttaaaatgag gtctacataa ctgttttaaa aattgcctct ggtaattctt 30180ctaagtctta cagtaaaatt tgccagaggc gtactattta aattacaatt aagctatcaa 30240atttaaaaat tactttttca tttcttttag ttctgttttt ctccccgtga atcaaatatt 30300gtttgtgcta ctaataattt tttgtggatt tgtcattatt tttgaatatt taaaatatcc 30360tgtactttgc tgtagtatag gtggcagtag tctaaacctt gatcttttta atgcattcac 30420atcaggattt ttatttttgt gggaaggtaa aaatcattat tttcccctaa ggtcagtgat 30480gtggtcttat ttttaagaag aaacatgcat caataagaaa cataaaaata ggctgccttt 30540gaaaggacta ctttatttaa gaagatatat ttccttattg tgtcttgtta gagggtggaa 30600ctgggccatt agtggacaaa agggataaga gggtcgagca tggggtttca cacctgtaat 30660cccagcactt tgggaggccc aggcaggcag atcacctgag atcaggagtt caagaccagc 30720ctggccaaca tgatgaaacc ctgtctctac taaaaataca aaaattagcc aggcgtggtg 30780gcacatacct gtagtcccag ctactcagga ggctgaggca ggagaatcac ttgaacctgg 30840gaggcagagg ttgcagagag ccgagatcat gccattgcac tccacccttg gcaacagagc 30900aagactctgt ctcaaaaaat caaaataaaa agggacaaga tatatctctt ttctgttcag 30960aaaataataa gtgagcccac tgataaatga cctgccaacc tcccagttct ggaagtcttt 31020tgtcctgctt ttggcatgca ctagtcaaca ctgtccaata gaagtttctc tgataatggc 31080aatgtttata tctgcactgt ccaatatggt tgccactagc cctgcctggt tattgagcac 31140ttgaaatgtg gcaactaaac aattgaatat tgagttacat ttaaatagcc acacttgtgg 31200cttctactag actcctttct gtacaaaatg tgctccctgc accaggagtg ccggcattac 31260cgtggagctt gttaggaatg cagtctcaga aaccacatct tttttcattt gtttttttga 31320ggtagagtct tgctctgtca cccaggctag agtacagggg cactatctgg gtaggctcac 31380tgcagcttcc gcctcctgga ttcaagtggt tctcttgcct cagcctcccc agtagctgga 31440accacagaca tgcaccacca tgcccggcta attgttttgt atttttagta gagacagggt 31500ttcgccatgt tggccaggct ggtctcgaac tcctagactt aagtgatcct cccaccttgg 31560cctttcaaag tgctgggatt actggcatga gccattgcac ctggccagaa cccacatctt 31620aacaagaacc ccaggtgaat taataaactc attaccattt agaagtcctc atagggctgg 31680ggagcagcct tgtagagctc atgcttactt aaaatttcca tcattcttta ggcattaaaa 31740aagtaatcca agcttacata tcataatttt taaagtactc tttataatta aactttctcc 31800cattgttggg catttaggtt gttttacaca tacacgcagg cacatatttg tatagataca 31860cacatatgtg tatatatttc aacatctata tctcgatatg taaatataga taggtacaca 31920tgtatatgtc acatggtgaa cattctctta cctaaatatt ttacgtgcat ctttttcttt 31980cacaaagatt ccagaaaaag ataattactg agtatttact tttaaaagag taatatgtat 32040tcatacactg cttttcagaa aggttatact aaatttagac ttccaacagc cagcaatgtt 32100tgagttccac tgtattacta ttatttaaaa atagttattt gatagacaaa aatattatct 32160cattcttaaa catgtgcata tttctgatta ctgttaagtt tactgttttt attttgtttt 32220ttttaattat actttaagtt ttagggtaca tgtacacaac gtgcaggttt gttacatatg 32280tatacatgtg ccatgttggt gtgctgcacc cattaacttg tcatttaaca ttaggtatat 32340ctcctaatgc tatccctccc ccctttcccc gaccccacaa cgggccccgg tgtgtgatgt 32400tccccttcct gtgtccatgt gttctcattg ttcaattccc acctatgagt gagaacatgt 32460ggtgtttggt ttttttgtcc ttgcgatagt ttgctgagaa tgatggtttc cagcttcatc 32520catgtcccta caaaggacat gaactttttt atggctgcat agtattctat ggtgtatatg 32580tgccacattt tcttaatcca gtctatcatt gttggacatt tgggttggtt ccaagtcttt 32640gctattgtga atagtgccac aataaacata catgtgcatg tgtctttata gcagcatgat 32700ttataatcct ttgggtatat acccagtaat gggatggctg ggtcaaatgg tatttctagt 32760tctagatccc tgaggaatca ccacgctgac ttccataatg gttgaactag tttacagtcc 32820caccaacagt gtaaaagtgt tcctatttct ccacatcctc tccagcactt gttgtttcct 32880gactttttaa tgatcctcat tctaactggt gtgagatggt atctcattgt ggttttgatt 32940tgcatttctc tgatggccag tgatgatgag cattttttca tgtgtctttt gcctgcataa 33000atgtcttctt ttgagaagtg tctgttcata tccttcgccc acttgttgat ggggttgttt 33060gtttttttct tgtaaatttg tttgagttct ttgtagattc tggatattag acctttgtca 33120gatgagtaga ttgcaaaaat tttctcccat tctgtaggtt gcctgttcac tctgatggta 33180gtttcttttg ctgtgcagaa ggtctttagt tgaactggat cccatttgtc aattttgtct 33240tttgttgcca ttgcttttgg tgttttagac atgaagtcct tgcccatgcc tatgtcctga 33300atggtattgc ctaggttttc ttctagggtt tttatggttt taggtctaac atgtaagttt 33360ttaatccatc ttgaattagt ttttgtataa ggtataagga aggggtccag tttcattttt 33420acttttattt tgagatggag tttcactgtt gtcacccagg ctagagtgca acggagtatc 33480tcggctcact gcaacctctg cctcccaggt tcaagtgatt ctcctgcttc agcctaccaa 33540gtaactggga ttacagatgt ccgccactat gcctggctaa tttttgtatt tttagtagag 33600acagggtttc accatgttga ccaggctggc cttgaacttc tgacctcagg tcatccaccc 33660gccttcgcct cccaaagtgc tgggattaca gatatgagcc actgtgccta gcgctgtttt 33720catattttta agccatattt ccttcttcct catttatgtg tccttggata agttatttaa 33780actatctgca ctttactgtc ttcatcttaa gttgctttgt aaggtcgctg aagaccaaat 33840gagttagtat atttcacata ctgggaacag caggtgctga ataaattctt agctactatt 33900actcttagtc cttggtatga cttttgtcca ctttctcagt tgagtagctg agactacaag 33960tgtgagtcac cacacccagc ttattttttt tattttattt tttgtaggaa tggagtctca 34020ctatgttgcc caggcaggtc tgaaactcgt ggcctcaagc gattctccca ccatggcctc 34080ccacagtgct aggattacag gtgtgagcca cagcacccag ccccatattt ccctaaagac 34140tagcagaact cacctcaggc tgcactgtca ggaggttgta aagaagcctc tttttgaggg 34200ttcctattat ctgtgttaga ggcagaaact tacccctctc ctttcattca ttcactcagc 34260agggatttat taaaccttta tgaaatccag gcactgcttt ctgctgctct gaactcaatg 34320ctgatttgtt attgattcta ctgctatatt ttggtctccc aaacaaggta ggacagcttt 34380ccttaagggc cttcaaccca ccatgatgat agcagctatt atttattgaa tatttatggt 34440aaggcattat attaagtgat gtacatatat tattccaaac aatgttttta tatgcaaaat 34500ttacagatga ggaattcgag gcttaatgag tttgaaaaat gtgcccaaga tcacatagtt 34560cataagaggt agaaccagaa ttcaagcttg ggccagccaa ctgcaaaatc caagcctctt 34620cattgcttta ctctgttcct cccatgaagt caccctcaca atttctcatt tgttctctca 34680agcctttgag gttagcctag atgttccttc agatttttca ttagtatttt tttgatttat 34740atttagttaa tgcataagat gttagaatta ttaatgcagt tgctatgtga tttgctttgg 34800gggaagtatt tatccagtag atctctactg aagacattgg tgcttgtacc ggttctctct 34860ccaagacaat actgatttcc acacttccaa aacaaagtta agatgtaaaa acagagaata 34920cagtgtggga ggaaatcttg aagacttgtg tagaggaata gaagcaaatg taaacaaaat 34980aaataaatta gcctccctct ttcacactcc catattgcat tgtaacactg tactgattta 35040gagaaaaaat attataatta acagcatatc agggatagat agaatggtag aggaagaaga 35100agctatacag tgattaaaga cataaagtac tgttttgatt gaggtggaaa agcctgcaga 35160gatgattcac aaagctataa ccagagcact ggatgagcaa gaaaagattt gaacatgagg 35220tgttgcctct gaagtttcca gaacagggaa ggcttaggag aaagtcagtg gatgaagaga 35280tgccaagtag accagaggga aaaatggaat cacaagatca cctttcaaag gccgaagaga 35340gagggattga tcctgaatta ataagaaaga gaggtgtccc ctgtgatgag tataaagggg 35400aacaggtgac ctgagagctg aaccagaggc aatatcaaat tagtcaaggc aaggccgggt 35460acagtggctc atgcctataa tcccagcact ttgggaggct gaggcaggag gatagcttga 35520gcccagaagt ttaagaccag actgggcagt atagtgagac cctgtcttta ttttttaaaa 35580aatagaaaat ttaaaagaaa ttagtcaagg ctaaggtggt gtaatctgaa taatcttttg 35640aagtcctttg tcacctagat aggctttttg tattctccac agtagttcaa cactctgcat 35700gatgtatgta gcatttcatg tcctgtgcaa cagcagaatc acaatgtagc actaaatgcc 35760actttcctta tgcacaccca ctgtccttaa tgtgttactt agcatcagct ctatgcatag 35820ggtacagcct ccttcccagt tttcagataa agaaactcag ccttagaact gtaatgtaat 35880ttgtccagta gcccctagac tctcagattt agtatttgtc agactacccc ctaaagacag 35940ggtactttag taaaagcaaa agcctgtgtc cctaagattt agcctaagga gaaagttcta 36000ctttctctgt ttgttaaaac ttgaaaatgc acacggcaac atggcaagac cccatctcta 36060caaaaattta aaaaactagc caagagtggt ggcacacacc tgtggtccca gctactcggg 36120aggcaggaag attgcttgag cccaggaggt tgaggctaca gtgagccatg atcataccac 36180tgcactcagc ctgagcaaca gagcaagacc ctgtctcaaa aaataaaaaa taattgggaa 36240gtgaatgtgt ccaagtgtct tcttctcttt tattttgtcc tatgacttct ccctcccaat 36300cctgcaccaa tgccataggg aaaaagatac atgtatcttt attaagataa agataatctc 36360aatgtgctaa tggggatgaa gtctgttgtt aaattcatca ttttctaagt cttctgagag 36420gtagtattta ctctttggat tgagctttca gacctatgca ttgggaaaat gtcaggccat 36480ttaaactaga aaaagtagct tttaattgat gaagaccttt gtatttattt tccctgagta 36540gcctcttgtt ccttaaaacc gggaaaatag aaggcaatat ttgtcacaaa aatacttcta 36600gcaagcaatt gaaaatttaa tttttctagg acccttattg gggttgtgca ggagcccatt 36660ctctgagtga catctctgat aatcgtaggc ctgagcatgt ttattcatac atctgaagcc 36720cagagagcaa atgcctgaac aaatggtatt gcctgtgtct acagttgaag gatggttcaa 36780tgttaccagt ttgttgatac gctgaaaacc accaggaaat tgcctccaaa tacctttgac 36840atagatttgg acaacagaaa tatttttaca tcagttgagg taactggatt taatcactgg 36900attaaaaacc acctttacta accactatag agaacagatg gaggttgctc aaaaaactaa 36960aaataaagct accatatgat ctagcaatcc aatttctgga tatataccca aaagaaaggg 37020aattagtatt tgggagagat atttgcactc ccatgtttat tgcagcacta ttcacagtag 37080ccaggatttg gcagcatctt aaatgtccat cagcagatga atggataaag aaaatgtggt 37140acatttacac agtggagtac tattcagcca taaaaaatca gtttctgtcc tttgcagcaa 37200tatggatgaa acttgaggtc gttatgttaa gtgaaataaa ctaggcacag aaagacaaat 37260ttctcatgtt ctagcttcca cttatttgtg tgaagctaaa aattaaaaca attgaattca 37320tggagataga gagtagaagc atgtaccaga gactgggaaa ggttgtggta ggggcaggca 37380ttgggggaca aggggatggt taatgggtac caaaaaatag ttagaatgaa taacacctag 37440tctttggtag cacaacagga tgactatagc caataattgt aagagttaat gaaagaggaa 37500agaaacacaa aatgtggctg gacagttaaa gagagaatta ttttagataa aataaacttg 37560agaggggctt ctggccaatt ttggtcagga gcactttctc ttacaggctt agagtggttt 37620tagggtgagg aggcttatca caagcttaga atgtttctgt gtgagggaga agttttatgg 37680tggggttgga ctatctctgc ctagagggga ggttatcttg gggcagacat ctttccagct 37740cggaggaggg ttatcttaag gctggcatct tcccagaggg tttatctcag ggctagcatg 37800tccccggtca gggaggagtt tggaatgttt ctgctgggag atgttatttg gtttatagtc 37860atgctgacct tagccattag gctgatgccc tttggattta ggcagttttt gattacggtg 37920aactttagaa tgagcggctt gtccaagatg gcgatgttcc tgctctgtca ataataattt 37980aattgtaaat tttaaaataa ctaaaagagc ataattgaat tgtttttaac ccaaaggata 38040aatacttgag gtaagggata ttccatttac cctgaagtga ttattatata ttgcatgcct 38100gtatcaacat atctcatgta ccacataaat atatacacct gcaaaaagaa aaccaaaaac 38160cacccgttct aagataccag tttaagtgta aataatttta attagtgggt atgtgagaag 38220gggaggagat ttcttgtact acatagatgg cttctaaagt taaactcctc cattacgttt 38280atagaaagaa gaattggagt aaggaatgac ttgataccaa tattaccaat atgtgtattg 38340acatcttgtg agctatataa tattatgtct ggtgtatcct gtaatacaga ggttgacaaa 38400gtttttctgt caagggccat gtagtaaata tatttggctt tgaggccata agatctgtgt 38460tgcaaccact cagttctcca taaaagcagc catgaacaat tcgtacaaga ctaagtgtgg 38520ccatgttcca ataaaattgt atttagggac actgaaattt gaatttcatg taattttcgc 38580aacatgagat gttcttcttc ttttgattga tttcaactac ttaaaaatgt ggacatcatt 38640cttagctcaa gaaccataca aagaggcagt ggatcaactt aggcccatga gctatagttg 38700gctgatctct gatatagtct gtgtggttag agcggggaag gtatttttat accagcaaaa 38760acgttatgct ttctccacaa gcctggtata aaaattggga atgcgctaca gatgccactt 38820ggtagaaagc tgttttatta aggtttctga aaattagact tgccaacaca aacattaacc 38880ttcgctccct ttattcagat gttacattta aatttttacc cagacctggg aactgagtaa 38940aattataatt atgatagaca cagggccctt gaactctttc ttttggctgt tttgaaaggt 39000cgttgtcttc atgttaatta ggagccagga acattctgct tcagctgctt gttgggaagg 39060cattatcatt agggaaatga tctaatcaag agtttgcatg ggaggtgggg tgtgggcctg 39120gaggctagga aacctgagag gttgaattac agggacacgc aactgataat tataataaac 39180ttctccctag acctgaatgc acagcttgga acctgtcagt tttcacattg gaattattat 39240actcaaccaa tgacagtgta tttggtgata tgtcaacatt ttcattctga atgagtgtta 39300attgggtatt ttagtgcttg aaattatttg ttaccaaaaa cactgacaca gcctgacatt 39360ctctgctttt tataattctg tagcacctat aaaatttctt tctctagatt taattgcttt 39420atacaaaata tgtacgtcaa ttaatcagag ctgtaattat tagactccac agttggccgg 39480gcatggtggt tcatgcctgt aatcttagaa ctttgggagg ctaaggcagg gggatcactt 39540gagcccagaa gttggagacc agcctgaaca acatagtgag accctgtttc tacaaataat 39600tttttgaaat tatacaggag tggtggtgtg cgtctgtgat cccagctact cgggaggccg 39660aggtgggagg atcggttcag cccagaaggc caaggctgga gtgagctatg atggcaccac 39720tgcactccag tctggggaaa ccctgtctca aaaaatagaa aatagactcc acggtggcct 39780gacggttaag tatgcctaaa tcagccattc acaggtcagg gggttctgaa aggggctctt 39840cagaattata aacttcaatt ttaaaaccaa aaattacctc ccttcctctt tctgctccca 39900actccttaga acaaatttga gttgagtgcc tgccagccca gggctgttct aggtgctggg 39960ggccaacagc acagaccagt gttccaaagc caggaacccg ggctttcttc cagcagactt 40020ttattgtaat gcatccattt gcttactaga gagaatttct tctgtgtgtc agttttttat 40080tgtaaatttt gaggcttact cttgagttct ccacctgaaa tcaaatttgg ctttgtcttt 40140tcttaaaaat tattttattg tggcaaaaca tacataatat aaaatttgcc attttaacca 40200cttctattta tttttattta tttatttttt gtttgttttg agacaggggt ctcactgtca 40260tccaggctgg aatgcagcgg tgtgatcttg gctcactgca acctctgcct cccaggttca 40320agcgattctc gtgcctcagg ctccctagta gctaggacaa caggcgttca ccaccatgcc 40380cagctaattt ttgtattttt tggtagagac atggttttac catgttgacc aggctggtct 40440cgaactcctg accgcaggcg atccgcctgc ctcagtctcc caaagtgctg ggattacagg 40500catgagccac tgtgcctggc cccattttaa tcacttttaa gtgtacagtt ctacagcact 40560aatccatcac attgtcgtac aaccatcacc actacccatt tccagaacat tttcgtcttt 40620cctggctgaa actctgtgcc cattaagcac taactctcca ttctcaccac tccccagccc 40680cagcacccac cattctacat ttctggacaa ggggacaggg tttcactctg tcacccaagc 40740ttggagtgca gtgatgcgat catagcacac tacagcctca acctcccagt ctcaaacgat 40800tatcccatct cagcctccca agtggctggg actataggca tgcaccacct cgcctggcta 40860atttttgtat tttctgtgga gacggaattt caccatgtta cacaggctgg tctcagactc 40920ctgggctcaa gcaatcctcc tgtcttggtc tcccaaggtg ctgggattac agctatgagc 40980caccacacct ggcccactat tctaccttct gttcctatga atttgactac actggggatt 41040tcatatacat ggaattatac agtaggttcc cttttgtgac tggcttatct cagtttgcat 41100aatgtcttca agatttatca gaaactttac attcttaaaa agttttttca atgtaaattt 41160ttagttttaa ttcaatcagg ttaaaggtca tatctcaaga atgaacagtg ttcctcattc 41220cttcttaaaa tatttttaaa atgttttttg aaccaccaaa tggcattttc attttgtttt 41280ttcttagcaa aaaaataaaa attaaaaggt cttattggtt gaaaaccctt gaatattaca 41340gtctaactag gagaaccaaa gtacattctt tgcatagggg tcaggaacta ttttaagaat 41400aaattcttct ttactttcta aagtaaatga gaaattgcta acttgaaaca tgtgtttttc 41460tttttttttt tttttttttt gagatggagt ctcgctctgt tgcccaggct ggagtgcaat 41520ggtgcaatct cggttcactg taacctccgc ctcctaggtt caagcgattc tcctgcctca 41580gcctccccag tagctgagac tataggcacg tgccaccacg cccagctaat atttttattt 41640ttagtagagt cagggtttca ccatgttggc caggctggtc tggaactcct gagctcaggt 41700gatctgccca cctcagcctc ccaaagtgat ggggttataa gaatgagcca ccacgccccg 41760ccaaaacatg tatttttgag gttaggttta gagaaagatg catcataata ttcttaattt 41820ttcagcgcat taaagaaaag atgaatagga aaggagataa ataacatccc aactggggga 41880tcctactttt aaagaaaatg tgtagacttt tcaccatgcg atcctcatac aatcttgatc 41940ttatattaat tagcatattt cccctatgtg cggaaatctg taatgccctt cctctgtggt 42000gggctgcctt gctttgaaaa caccaccagg agcccctctg aagaatgaca gcagcaggtg 42060ttctgagaga aagtagtgag atggacagag ctgtggatac taactgcaga gaccaagtct 42120ccagacacag ggccagctgg ggaaattcta ctgctggctg ccattccttg gttctgttgt 42180cagtaatcac aggtcacaag cctcgcctct cctgcctact agatggttca ttttggccgg 42240gtgcggtggc tcacgcctat aatcccagtg ctttgggagg ccaaggcctc ccaaatgtat 42300gaactctcgt acattactgg tatataaatt ggtaaaacca tttggtaata tacctattaa 42360ggctaagcat gtgtatgccc tatgatccag gtgtcaaaag acatgtataa gaatgtagca 42420gggcaccgtg gctcatgcct gtaaccccaa aactttggga ggctgaggcg ggtggatccg 42480gaggtcagga gtcaagacca gcctggccaa tatggtgaaa ccccgtctct actaaaaata 42540caaaaattag ctgggcgtga tggcgtgcac ctgtagtccc agctactggg gtggctgagg 42600caggagaatt gcttgaacct gggaggtgga ggttgcagtg agccgagatc aggccactgc 42660tctccagcct gggcaacatg agtgagactc cgtctcaaaa aaaaaaaaaa aaaaggttca 42720ttttaatgta acttagttga ctgtagaatt tggaatgtga agggatagga ttgtattaat 42780gagtgttgtg ttcgtatatt acatcatgct gtacccacag aaacaggtcc taagaaatgc 42840tattctctaa caatacacat tcatgatgtg gctcacccag agcaccaaaa ccctgctggc 42900catagcaggt agggtccact tatccccggc atcgcctggg acatcatatg gacatgttct 42960tttacttggt tcttcatgac aagcacaaaa gcataacttg agaaggattg aacttacatt 43020agaatgagac ttttatgtat ttttataata tcgtcatctt aagcaaaatt aaatgtgtta 43080taaatgatat atggtggacc tctgatgttc atgtgtgttt ttcacctcac cgaagaatgc 43140taaaaattaa tcttaaatat agcaaaatta cagcaacaat aataataaaa tatacttgct 43200cagaatggat cttgtgtcct ccagcattgt ctagaatttt ccagattttt caggagggct 43260gtctcgtctc ataactctgt gaatgtatat cactccagaa ctgccttttt tctctgctta 43320gacagttgta tctttctttg tattgagtat tcttcggtga gttagctcag tgcctctttt 43380tttaaaattt tttattttca ttttaagttc ctgggtacat gtgcaggatg tgcaggtttg 43440ttatataggt aaatgtgtgc catggtggtt tgctgtacct atcaacccat cacctaggta 43500tgaagcccag catgcattag ccatttttcc taatcctctc cttctccctt ccccacccac 43560caacaggccc cagtgtgcgt tgttcccctc cctgtgtccg tgtgttctca ttgttcggct 43620cccaattata agtgagaaca tgcagtgttt ggttttctgt tcctgtgtta gtttgctgag 43680gatcatggct tctagctcca tccatgtccc tgcaaaggac atgatctcat tcctttttat 43740ggatgcatag catttcatga tgtatatgta ccaccttttc tttatccagt ccatcattga 43800tggacatttg ggttgattcc atgtctctcc tattgtgaat agtactgcaa tgtacatacg 43860agtgtgtgtg tctttgtaat agaatgattt atattccttt gggtatatac ccagtaatgg 43920gattgctggg ttaaatggca tttctggttc tagatcttta atgaattgcc acaagcttgg 43980tgtctcttct gtgttttcct agcccacagg atggcacggg tgctgtttca ctcatgatgg 44040catttcccac tggtaggaaa tatgcatgtt atattatatg tgacaaaatc catggaacac 44100tcaccaaagc cacagaagtt gtatttttac ctgcttaaca gccggtgtca tccaaggttt 44160gatgtgacag acacatactt tattctgtta gctacaaata tttattctgg tacaggatgt 44220gtttaaaggc ataggcaaag gccacattat acaggtaagg agcatggatt ttaagtggaa 44280tagaaagaaa ttgaagtctt ctagatagag gaatgaaacg atctgctgta tgtctgaaga 44340tgaacctggc cactggaata tagaggggct agcagaagtg ggtataagca gagagactaa 44400ttacaaaggc accgtaagag tctacgcagt acaggtgagt gacttgacta gggttgtggt 44460agtggagtgg gaagagtgag gcttgttatg ttcatagagg tgtataacag agcaatatcg 44520tcttcatgtg ccaagccctg tgctaggtct tggggattca gcagtgacag aggcaacaca 44580aatccctact ttcatgaagc tttcatatta gcagcgaggg gtaaaccata aataaataca 44640taagtatgta gatggtgcac cagaaggttg gggagatatt aagcagagaa gaggaataca 44700gagtagcgaa tagagtgcca ttgttttttt ttaaatggaa tcttgctctg ttgcccaggc 44760tagagcacag tggctcaatc tcgactcggt acaacctccg cttcccggat tcaagcgatt 44820ctcccgcctc agcctccaga gtagctagga ttacaggcac acaccaccac gcccggctaa 44880tttttgtatt tttagtagag atgggatttc actatgttgg tcaagctggt ctcaaactcc 44940tgacttcagg tgatctgccc acctcggcct cccaaagtgc tgggattaca ggcatgagcc 45000accacgccca gccatagaat gctgtttaaa atgccacagt tatttatgta aaatctgtga 45060tctttcacat tgttaggaga aggtaaaaag tgcatccaga agcaaggtta tcagggccaa 45120agaggcaccg tgttcccaag gaacctgtag tcactggtag agtcagtact gagactcccg 45180aagatcaata ggaccagcat atggtgtctt ggccatatca tcacgatctg tggtcatgtt 45240ttgagggcaa cttgaatatt tttcatgata aaaatatagt gtacctcttt ttctgtaatt 45300gaaaaacaat ttccaagtaa tggctgtgta tattacgtga aaaattacca cagaaatcga 45360gagtggagaa aggtatccca agtctttcat agaaaatgac ctggaaattt tccttttaaa 45420tgtctctttt taaagtagta tgaatatggt tggccaggcg cagtggttta tgcctgtaat 45480ggcagcagtt tgggaagcca atgggggtag atcgcttgag ctcaggactt cgagactacc 45540ctgggcaacg tggtgaaacc ctgtctctcc aaaaaaagaa aaaaacaatt agctgggcgt 45600gatggtgcac atttgcaatc ccagctactt gggaggctga ggtgggagga tcacttgagc 45660ctggaaggtg gaggttgcag cgagctgaga tattgccatt gtactccagc ctgggtgaca 45720gagtgagacc ctgtctcaaa acaacaacaa caaacaaaaa acaaaaaaaa cccacaaaac 45780caaaaataaa gtaatatgaa catggtaaaa aaaaaaaaaa tcaagcatta caagaggtta 45840cccagtgcca ggttaagtcc ctcccatctc tggctgctgg ttcctcagtt ttcttcccca 45900caagcaacca cggtgaccag gttcttggtg tcactctggc attgtggttc tcaaccttgg 45960ctgaacagta gaatcacatg cagaactttg aaaagaacag ttgccggctg ggtgcggtag 46020atcacgcctg taatcccagc actttgggag gccgaggtgg gtggatcatc tgaggtcagg 46080agttcgagac cagcctggca aacctggtga aaccccatct ctactaaaaa tacaaaaaaa 46140attagctggg catggtgacg gacacatgta atcccaggta ctcgagaggc tgaggcagga 46200gaatcatttg aacccgggag gcagaggttg cagtgagccg agattatgcc actgcactcc 46260agcctgagca acagagagag actctaagaa ggaaaaaaaa aacggttgcc ttatcccacc 46320tccagaggct ctaattaaat tgcttttgaa tatgccatag agatttgtaa tacccccccc 46380cctcgccccc cacaggtttt aaaaatgtgc agccacttaa atgttctaga gctagcctac 46440atactcataa atgtatgcat tctttaaaat gcacttatca aaaaattagc tgggcgtggt 46500ggtgtgtgcc tctaatccca gctactaggg aggctgaggc aggagaatcg cttgaactca 46560ggaggcagag cttgtggtga gccgagatcg tgccaagccg agatcgtgcc actgcactcc 46620agcctgggtg acaagagtgc aactccatct caaaaaaaaa aaaaaaaaat ttattcaaat 46680gacagcaaaa cggtgtattt ctaccaatga tagaaaaata ctctctgatg gttttttctt 46740tttctgatgc ttttttcttt ttaacctgta aaatacatct tggagattat ttcacattag 46800catgtatcgc tagagataac ttattttttt atatgtccaa atgatatgcc atcatatgga 46860tgcaacataa ttttatcagt gccctactga tagttatgtt attcaaagta atctgttatc 46920acaaatagtg ccataacaaa tattcttata tatatatcct tatgtacaca tttgtatata 46980tctataaaag tcccagaatc agaattagaa ttatggatca aaggtaggtt ttcagttttg 47040ataaattcta ccaaatttta tccataaggg gcaatgtgtg agaggaccac actaacagtg 47100cattcgcttt gcttgtaatc tttgccaata aaaattaaaa ctagtgttgt ctttgatttt 47160gccctgtttt aacatcagga ctaaacttag gcattttcca ttagcttaaa accgtttctt 47220tttctgggaa tgtctctcct tcccctgctt ttttggccta tttttctagt gggttgttgg 47280tgtttttctt attgattata agatgtatta atatatgaag gcatcaaact tttgtcagca 47340tatgatgaca tattctgtat atatcatcag tatatgagca actttactgt tgctctttta 47400tttatgattt tttttttttt ttttttttga gacggagtct cactctgtcg ccccaggctg 47460gagtgcagtg gcgccatctc ggctcactgc aagctccgcc tcctgggttt gcaccattct 47520cctgcctcag cctcccaagt agctgggact acaggcgccc gccaccatgc cctgctaatt 47580tttttgcatt ttttttagta gagacagggt ttcaccgtgt tagccaggac gggctcgatc 47640tcctgacctc gtgatccacc catctcagcc tcccaaagtc ctgggattac aggcgtgagc 47700cactgcgccc tgcctttttt atgattttta atctgatata gtaaaatgta tagcttttat 47760gttttggagt gtttcaagta tcagttggta agagagctag gtactggaag ctattggaga 47820acagagaata tgtcatctat attcttagat cctcagtgcc cagcacatgg tagataattg 47880ttgacagaat agttggatgt aaatgtgatg atgatgatga aggatcatga aagttgatcc 47940caggtattca tccagaagaa attaaggcag taacagtatt atcatcattt aattcccttc 48000ataattaaac gctccagcgt ataatcacgg tgtataatac ttctggtata aactgaaagt 48060actgagtttc agaaggcaga tctagctgag tcgtctcaga gccgtcatct tggtcactaa 48120gctcttgctc cttagaaact cgaagatcct ccaccccttc ccattggtct ttctgcctcg 48180gaatgttgtt cccttgcctg tatctatgac tgtttcctgc cagctgcaaa acacagccta 48240aatatctatt tccctttaga agcttcttct gagtcccatc ttcatctccc tgccatcttc 48300cctcatagtg tcccatggct ttcctttata gtagttatca gaactgtcat aaaataatgt 48360tttgtgttac agcctctgtg atgtgtctcc ctcactcgac tgggaacccc aatcacttct 48420tcttccctca tgtccactgg gagtgctctg ggattgggga acagaggtaa acaaaaaaag 48480ctccctgccc tcctttgagt attacattct actgtatata tgtgttggga aaaggagata 48540ataataatac tgagttgtgc atcatttcca atgattgtca tttaaatgaa gcagaaaagc 48600cagtaggtgc atttttggct tggagtatct gagtctggtt tgtgtcttaa tagtagaagg 48660atctagcaca taacatatgc atacactacc aggctgtgag actgtggtca ccctcgtgca 48720agtcaacaga cagctgtcag catagtcacc gagccatttc tttctctctt tttttttttt 48780tagcgacagg ctctcattct gtcactcagg ctggggtgca gtggtacaat caaggcttac 48840tgcagccttc acctcccaga ctcaagtgat cctcccacct cagcctccca agtagctggg 48900actacaggtg tgtgctacca tgcccagctc gttttaaaat tttttgtaca gatggggtct 48960cgttatgttg tccacattgg tctcaaactg ccgagctcaa gcaatcctcc tgcctcggcc 49020tcccaatatg ctgggattat aggagtgagc cactgcacct ggcccaagcc atttctcatc 49080cttaccaaaa tgtattctta ccaaactgtc aggccatttt tgccctgctt gatggaagtt 49140ttgctatcat taaagtccat tacaccttca aatgcaaaat agagcagata ttttcatgca 49200acaaaagatg ctttttcatt tgcaagacaa gaaataggat ataaactact tttgaccaat 49260ggtatatgtc cagtatttta ttaaacgttc tgaatatagt ctagattttc taatattaat 49320attatatatc tgtggcacat ttccagctaa ataatctaaa atttacagcc tttttaaaac 49380acagaggtga atgaaccaga ttgtctctag ttctgaaatc acctcctgaa ggtgggtttg 49440ctgcttaagg gatacattaa ctatattagg tccctagggt actttccagt gctttatttc 49500tgagtactcc acaccactgg catttttcca gacaaatccc tgatggtgta gcctgttttt 49560ttaatattaa gtctctctga accaaagtca tatctttcca gaaggttaga cttttagaac 49620agtatattaa tattttacta ccaaattact gggtcatttt ggcctgctta agtacctaac 49680ctagtaccaa caaattattt aagcaatcat aggagaaaaa tccagtattt caggtagaca 49740cttaaataat catttctata cattatacac agagaaattt acttcataat cataaaattt 49800tatgtctaag tacttttctt taattatttt cttgcctgtc ataattccca tagcaacctt 49860tactttgttc tctgtttttc atgtgcataa agaggctttc aggagacctg gtccttgcct 49920cccttcccca tatgcaaaca actacgaagg tgaatcaatt acagaaaata tgtataaatc 49980atctctttta caacatcaaa aatattttca gggtactttg tgagaaattt caattgcaat 50040aggaagggcc ttttattcac aaacatagga acattacagg aaaagaattt aactgaactc 50100ccaacatttt ttgagaaaat ggaatccaac cttatctctt ctgttactgt ttttgttttt 50160ttgagatggg agtctcactc tgtcacccag gctggattgc agtggcatga tcagttcact 50220gtagccttga actcctgagc tcaggcagtc ctcctgcctc agcttcccaa gtagctagga 50280ctgcaggtgt gtgccaccat gcctggctca tttttaaaat gtttttgtag agacagggtc 50340tccctatgtt gcaaaggcag gtcttgaact cctgggctca tgcagtcctc ccacctgggc 50400ctcccaaagt gctgggatta caggcatgag tcactgtgcc ttgcctctgt tatttgtttt 50460acatacgtga atgtatgtat atatagaaat gattcagttt tgtttttgtt gttgttttct 50520ggttttatta aatacagtca tcataaacat ttgcctgtaa tcatctttag tctttgtaac 50580tattttcaag gttatctaat agtctactaa gcatgtgaac cataatttta aaattttctt 50640aaactagttc ctagaagtga aattaatagc ctaggtacat ctaaatgcat gtctagttac 50700acatagaaag taaattgtct tcccaaaggg ttgcgtgaaa gccaaaaact atcaacaagg 50760tgtgaaagta tcaatttaag tgtgtccccc tgacattgtg aattgggact ttgcaaagag 50820cattggttta atgatggaaa atggtattgt gtttaattta atgaggttga acatcttttc 50880cagatgctta tggctaattg attttttcgt gaattttcta ttaatatcct ttatttatat 50940caattcattt cttactgttt tacttatcaa ttagtgtgcc ttttatatat gcaaagaaaa 51000tgttaaacag taaaatattt aaggactgaa gccatatccc ttttcacaag tgtgtagttc 51060cctgcagaac acagaagtta gctgttcatt catgcctgta gattaattgt tcattgccac 51120ccgggagaac tcatcttgga aggaaagagc ccagattcat ttgtttgatt gtttcgctgc 51180tcaagaaaag gcctggcact tggatgtacc atcttttgac atggcctttc ttttcttgag 51240acggagtctc actccttcgc ccaggctgga gtgcagtggc atgatcttgg ctcactgcaa 51300cctccacctc ccgagttcaa gtgattcttc tgcctcagcc tcccgagtac ctgggactac 51360aggcacccgc caccatgcct ggctaatttt tgtattttta gtagcgatgg gggtttcacc 51420atattggcca ggctggtctc gaacacctga ccttgtgatc tgcccacctt ggcctcccaa 51480agtgctggga ttacaggcat gagccacggg gcccagccaa catggtcttt ctttggtagt 51540agagagtcta ttaagatatt ctagagtttt atatttattt gtccagttat tcagttgcat 51600tcctttatat tacccaacat tgcttgaaga aatatcttct ccctgtattg gcttgatttt 51660tgaaacattc tgaattatta taagttatat gtatagtcat attttctgct gtaaatttga 51720actccatctc tataggaatg tcaggggtag tgcataagaa gtaaatcaac agtcactcac 51780acaatatgtt atatttacaa tatattcgca accatctgta agaaaatttg caagtgcatt 51840ctttctagta gcttcaattt agtatactca ttttacaaat gagagaatta gccagagaat 51900tatttacatg ccttgccttt ggctatgcaa tgagtcactg gtacttttag gattggaact 51960ttaagttcct ggaatgaaga agcagaatta atgggaaaag aagcttcaca aaaaataagt 52020tgtaatcata ttttcaaaat ataaagtctc tcttagattc actggaaaaa ggatgctaaa 52080caaatttcaa gtacaatgac tgtttttgga gtggcttgct tgttatgaaa catggttatt 52140taatcaactg gcctatagtt gacatttgca ccaagttaat taagttggca tttgtggagg 52200tttgtgataa aacactgggg atagcttcaa gctgaagcct ttgttttatt ctttattcat 52260tataaatatt tatcatccgt tcatcatgga aagtcacatt tctgtttgtg ttttttaatt 52320aagaaagaga cactagcaca tttaggaatg tttaagaatg tttaggaatg cgaaaatgga 52380tcgggagagc tttcttgtaa attgggagag ctttaaacta taggatgtaa gagagaagag 52440gttttctctc tttcagtcct tattcattca ttccacaaat gtttgttgag catgcattcc 52500atgcctggca ctattccagg cacttgggat ataggagtaa gtgaaacatt taaaaaaatc 52560catcctcgtg ggtgttatat cctaatcaag ggagacatat actaaacaat atcgtaagta 52620aattatatgt tagaaggtaa aaacactaat aggctgggcg tggtggctca cacctgtaat 52680cccagcactt tgggaggccg aggcacatgg atctcttgaa tccaggagtt cgagaccagc 52740ctgggcagca tggtgaaact ccatctctac agaaaatacg aaaaattagt tgggcatggt 52800ggtgcgcacc tatagtccca gctactcagg aggctgaagt gggtggatcc cttgagctca 52860ggagacagag gttgcagtga gccaagattg tgccactgca ctccagcctg agtgacagag 52920taagaccctg tcaaaaaaaa aaaaaaaaaa aagaaggtaa aagcactatt tttaaaaaag 52980tagaacaggg taagtgagat tggaaatacc gctgcaatca atgggggtta caatttgaaa 53040caggatggtt gcagaagacc tcattgagaa agattttgag caaagactta gaggaggtga 53100tagagttaga agggtagaaa tctaggggag gagagtgtct ggtacctctg aggaatatca 53160aggggcccaa caaggttaga gccgaaagaa gggagagcga ggtggcaggg agagggtgta 53220gagatcagcg gaagaggacg ttactaacaa aggagactta gaaggaacag ccaatgggaa 53280aagagaaaga ccaagaggga gtggtttctt ggaagtcaag ttgagaaaat gaagagagga 53340caggggatga tcagtgatgt taaaggctgc tgattgctgg agtaagatga gcactgagaa 53400tcagccatcg atgtagcctt agagacatca ttgatgacct tgatgaacag ttttagtgga 53460gtggtaggag gaataggagg aagtatattt aagagagaac aggaagagaa gagttagagg 53520gagcagagcc aactttttct atgcattatt ttttctgcaa aggagagggg aaaactggac 53580aagagctggc agagaaaatg gagtcaagga aaggttttct taggaagaga gaaataatca 53640cgtgagtttg ctgattcaaa tgattcaata gaaaaaccct aagtgtagtg acatccatgc 53700agtttctgga gtacctgtaa ccagcatgaa tttctagacc aaaaattccc tacatacaat 53760ttattcactc cttttctccc atttcattga attttctgca gctatgtatt gagacagttc 53820aactctccca agccactatt tttctgctcc ctgcctactt gttttcaatt tcataactga 53880taagatagtt tgtatataaa aaattatatt agcatgaaaa aattcttgtc atggctggat 53940gcggggctca cacctgtaat cccagcactt tgggaggccg aggcaggtag atcctctggg 54000ttcaggagtt caagaccagc ctgaccaaca tgatgaaacc ccatctctac taaaaataca 54060aaaattagct gggcatggtg gtgggcacct gtaatcccaa ctactcagga agctgaggca 54120ggagaatcgc ttgaacccgg gaagcggaga ttgcagtgag ccgggatcgc cccattgtac 54180tccagcctgg atgacagagt gagactccat gtcaaaaaaa aaaaaaaatc atgtcatctg 54240tcatcatcag tagatatata ttaaaggcct agaaaatttt cccaaaacac acatacacat 54300acataatgtt ctaatttttt aaaattactt agcctatgac tgaaatccaa aactcaaaat 54360aatatcaaaa ttgcccctct aattttaact ttttcaaaat ggttcctttg gacaatgtca 54420aattttgcaa atattgagca gaggctcttc tataaactgt aaatagccat cataccctca 54480tgttaaggaa taatttggtg gtaacagcat tttgatatgt tcgtttttta aaaaaatgaa 54540agtcacatgt ctgaacttct tgcctactat ttgtacttta taataacttc agcttcagta 54600ctgaaaacca gcaactgttc cttttgaact gaaagaagac acttgaatcc tagccacttt 54660caggcaggat gagctgccga ccagtgctac tgaaggccag cttgccaaag ttctattcat 54720tttcagcaaa gagagaaaaa ccttaaatag ccggtgtctt ggcttctatt gttttccgat 54780tctaatctac tttcccttgt agataagtaa tgaaaagaat gcttggtgat gctgagatac 54840tctgaagtga ggaaaatttc agaggagcag ccaagtcttt ccacaaaaca gcattctatt 54900aatgaatgta aaatgagccc tcggtgtact ttcgttgtgc ctttttggaa agataaaatt 54960tgaaattttt gtttttccaa gtaggagtct cactctgttg ccaggctgaa gttcagtggc 55020acaatctttg gcttactgca acctctgcct cccaggttca ggcaattctt ctgcctcagc 55080ctcccgagta gctgagatta caagcgccca ccaccatgcc tggctaattt ttgtattttt 55140agtacagaca gggtttcacc atattggcca ggctggtctc gaattcctga cctggtgatc 55200tgcctgcctc ggcctcccaa agtgctggga tttcaccgtg agccactgcg cctggcccaa 55260attcgaaaat ttatagatgt gtgtgagaga gagcaagcga gcgtgcctaa agcttagcaa 55320agacatagct aagcattacg aaactatcac gatgccttta tgatcgttag aaatggctat 55380acctatgacc aacaaacaga aaaaacatat gcttttgatt aacaaaaaca tcagatgcat 55440gtaggaattt gtcctagctt ttctttttat gagcagacac atgcatggca cttgaaattt 55500gactgctaaa ggtttactcc taactctgtg gatccaattt gttatatact cattttacaa 55560agggaggaat tagccaggga gttatttata tgacttgcat ttggctatgc aatgagtccc 55620ctgcttcgtg gccttgctca ggtagcttct ctatgtctca gttttctcat cagtaaaaat 55680gaagataatc gaaattacat taggtaatac ttgtgaatca gcacagtacc tgacacagtg 55740taagcattta tgaaatattt gcaattatta tcattaataa gatgtattat tatgtgcata 55800gcctgatctt aattgattat cctcattatg agtataatat aaaacaatct tcaaaacgca 55860ctcttttagc ctctaagtaa ttgtgctgga ctgagaagga agaaaataca agaactctgt 55920ttatattctc cggagacatc atgtttccag taacgtggag ttcactaaca acaacttaaa 55980tgtaactccg aattaaggaa ctcacacagg ctgaaggagt ttttctggga ggtaatccca 56040tgttgatgga gtctcacatg tgtattccag gagtgcctga gtatatatca catgcagagt 56100gagagaaaaa gagaagggag agagagagag agactgaaca agtttaaata aatcttacta 56160aaaattaatg ttagcatgtt agatacactg acctcttttc aattcctcag acatgcaagc 56220atttcttgta cacaaggcct ttgcatatga tgttctttct gcccagacat ccctcttctc 56280aatgcgttgc agggctgagt tttttctttc atcgtttaga tttcagctta gtgtcattgc 56340agactgtcat ctcattactc taactaaaat accatccctc ccaccatcat tttattatct 56400cagctttttt tcttgtctct catagccgtt atttttctgg tgccttttaa aaaaaaatca 56460gtttccttca ctgagatatt aagtttcatg acagaaagct ttaatctttt ttacagccct 56520gttttcagta tttagctcac agttacagca cagagtttca aagtaataag tactcaataa 56580ttgcatgtag tttggggagt tgttaaataa aatttaatct cttcattagt acatcctata 56640gctatatgtt tataaatttt catattgtct tgagaaaatt gaaactgaca ttttggcttt 56700tacaggatca taaaagtatc acacagggat gaccctgcag cgatgtattg ctatcacctc 56760tgttactatt ttgacactca gctaaatgat aggtcaatca catttgagat tttcatcatc 56820gtatgaattt gaataataat tacacgacag tttgcaaact aaggtataca atgtttatgc 56880aactgtcccg catcttggca cgctatgtca tttttcagtg tttagagtca ggatctctca 56940tcagtacata gttgatgatt ttattgttat tattttgaga cagggtctcc ctctgtcacc 57000caagctggag tacagtgttg tattctctgc tcactgcagc ctctgcctcc taggttcaag 57060cgattctcct gcctcagcct cccgagtagc tgggaccaca ggcacctgcc accacgcctg 57120gctaattttt gtatttttag tagagatggg gtttcaccat gttgccccgg ctggtcttga 57180actcctggcc tcaagtgatc tgcctgcctc tgcctcccaa agtgctggga ttacaggcat 57240gagccagcat gcagcccata gtttatgatt ttatttatga agaaaccagt aaagcagaga 57300atagcaattc agatttcttg catctacatt gcaccagaga acagaaggta attctaaact 57360gtgaaaagag atgacaacac catgaaaaat gaaagaatta tctcccttcc cccacagaca 57420atacaagata gaaccctttc cccttggtcc tggcccgctg aagtctatcc cattttgatt 57480gatgattgat tgagacggag tctcgctctg ttgccaggct ggagtgcagt ggcacagtct 57540cagctcactg caacctccac ctccagggtt caagcgattc tcctgcctca gcctcccgag 57600tagctgggat tatatgcatg tgccaccaca cctggctaat ttttgtattt ttagtagaga 57660cagggtttca ccatgttggt caggctggtc tcgaactcct gaccacaggt gatccaccca 57720cctcggcttc ccaaagtgct gggattacag ttgtgagcta ttgtgcctgg ccccattttg 57780ctttatttta aaatatgtgg gcctgaccag caagcaatga gtttgccatt gggtcagcat 57840cgtcctatgc atgaggaggt ggtctaaaat cttgaggatt ttgatgagtt atagctttgt 57900ctagaatcag actggccttt ggattcataa aaagttgatt ttgctcaaga acatggcttt 57960aaggcttcat ataaagattt cctacagaaa gagaagaaca caatttggga tccaagatgc 58020cgctatgaaa taggcttaca actcttatct tttgggcttt aaaagagaaa aatacaagaa 58080tgctccaaaa tactgatgct gaaaatatag atgactgtga aacagcagag tacagcatca 58140aaacagcaaa gtatataaat tttttaaagc attaaagaaa aagggtggcc aggtgtagtg 58200gctcaagcca gtaatcccag cactttggga ggccgaggtg ggcagatcac ctgaggtcag 58260gagttcaaga ccagaccggt caacatggca aaaccgtctc tactgaaaat acaaaaatta 58320gccgggcctg gtggcgcatg cctgtaattt cagctactct ggaggctgag gcaggagaat 58380cacgtgaacc taggagacag aggttacagt gaggcaagat tgagccactg cactctagcc 58440tgggcaacag agtgagacta tctcaaaaaa gaaaaaagaa aaaagaaagg gtgacgcttt 58500cttgtgtgaa cgttagacat aaaagtggaa aaacaatgaa accccgtctc tactaaaaat 58560acaaaaatta gccgagtgtg gtggtgcaca tctgtaatcc cagctactca ggaggctgag 58620gcaggagaat tgcttggagc caggagatgg aggttgcagt gagctgagat catgccattg 58680cactccagcc tgggcagcag agcaaaagcg agactccgtc tcaaaaaaaa aaaaaaaaaa 58740aaaaagtgga aaaagaaatt gaagagtaga taaaagcata aattaatcca gtgatatgca 58800attttcctat gagcaatgaa cccacaaagt gagagctcta aggataaaag gtgatgatcc 58860tgggaggaac tattagtaca cagtagtaca agttacattt tcagaaatgt ccaaatagaa 58920atgcaggtat taagatgtag aaaaggaaat ctgggtagct gaaatgtgat gcactgacaa 58980ggacaaatac tgcaaaagac cagctaatca cgttgtaaga gccctagaga tcaagggtac 59040attttgtaat gaacctattc cggagttatg gaaacgaccg ccagctgttc ctgactgaag 59100actacttgag gcacgatgac ccagaaagtc catatgtttc caaagataaa agcttaatta 59160gatatatcac ttctaactga ataaatccac actcccacaa gaaaggagat taactgtaag 59220gcatatgtca ggagggtgac tttcaaacat gcaacagctt cagatactga aacatgctac 59280tatttaactg tggctgtctt tggatgggag actttcaggg gatcgttatt tgtgtgtctt 59340tcaatgctgg gtgcatataa cttctataat gaaaaatatt ttgaggctgg gtgaggtggc 59400tcacacctgt aaacccaaca ctttgggagg ctgaggcagg aggattgctt gagcccagga 59460gtttgagacc agccagcctg ggcaacatag tgagacccat ctctacaaaa aataatttaa 59520aaattagcta ggaatgggag cacatgcttg tattcccagc tgcttgggag gctgaagccg 59580gaggatcact ttcgcccagg aggtcaaggc tgcagtaagc catgattgca tcactgcagt 59640ctatcaagcc tgggtgacag tgagactgtc acacacacac acacacacac acacatacac 59700acacacacac acacacacaa ttgtttttgt gtgaaagtat atactatcag agatacaagg 59760aatgaaggta atccagctct ttctatgctt ctatgaacta ctctaaagat caattggtag 59820tcacatcctg tattataaaa tagtcaagcc attgccaaaa gtcattgaag agatttttgt 59880ttttaactgc tatttaaacc tggaaacagg tctcaaagtg actatgcaat gtaaaagctc 59940catctgaata ttagcaaagg actttttata tcttatttta aaataatgaa tgagaccggg 60000aatgcgaccg cgggaatgag accgcacaaa attatgtgtg ggtgttatgc ggccctctct 60060ttatgtactt cctgtcattg ctgggttctt actgatagaa gctttgtctt ttcattgagg 60120tgtgacttga acctgtagcg gggtgattgg gtctaacatg gcaagactgg tgacagtggg 60180gacaggggcc ccttatgggg tataattatg cttcctggta attttctact gtgctgctac 60240tactataaga agccctctag gaagatcagg aatcccctac caggtgtgta tgtgttgaca 60300cactcatttc tcccttgtaa atttgcttga acgaagagtt tgcagtgcat ttttatcccc 60360catgcatttt acagtcacat cataacaata ggtgatctat taatttacgt caagatacca 60420caattgaggt acaactgatc actaactttc attctgaaac ccctattcta cgtattttct 60480agtatttggg tcttgaagat tatattagtg cagagtgcca ggtggggagg gaggaagggt 60540agaagggagg aggagggagg aagacttttt gagatagggt ctcactatgt tgcccaggct 60600ggtctcgaac tcatgggctc aagcaatcct cccactttgg cctcccagag tcctagggtt 60660acggtcgtga gccactgcag ccagctgtgg ttcttaagta ttcttaagaa cacttttttt 60720tttcattttt atttgtacaa aatattctcc caccacactc aaacaaatgc agtttagact 60780acaatgagag cattttgttt atgtcaagaa aacaaagtgt atatttctta ataagtaaac 60840ttttaaaatg taatttcatt agtacaggaa attaataact ttagtgtctt tccttcttca 60900gcctcactca cctcatctac aagcagagtt gggactcaat tattctgtga attttaattc 60960caactaataa attaaccaag ctttgaaatt tccttgtaag tatggttata acaattcaca 61020ctgctgttta tgccagtggc tttatttcaa atggtagttt ctcagttgga gaagcagttc 61080gaagtcattt tgagctcctt taaactcaac gctttataaa gcggtgatta ctttcgtgtc 61140ctaattaaat ggccacgtca ctctcttgct cttgtttgga aagtcacaag caataaagaa 61200ctgtgctata ttatgactga tgagcattat ttgatttttg ctatgcaata ttgcatatga 61260tatttactac ctgtgaacct ccttgtggta taggacacac ttagtggagc aggtaatgga 61320aagggattat taaaggaaac gatctcatgc tttgtttttg agagaaggtt tcatctgaaa 61380cagaaacaga cacacatacc acgagcccga ttactataga accgaagaga cctttccagc 61440tgtttacact tcggatgtaa aatggcaatt tgacatagac taacagtgtg atgtgagcat 61500ctgcgtaaaa aggcgacata tggagcatgc ccagtgtggg agtgcgtcct cctccctccg 61560cctcccccct ccccctcgag atctccaaag ataaggcttg aactttgcac ttgcaaatgt 61620cactgcatac gtttttgcac tagttttttt tttttttttt ctgcgattct tttacaacta 61680atttctttta acaatttgag cgcagggaag agaatgttta gggttatgag atgcattaag 61740tttagaacga gttgatgctc ggcttttgaa tgcacttgtc ttctttattt ttaaagcaat 61800ataaagcact cgagtgtgtg ttttcagccc ctcctggaat gggaaaataa gaatctccct 61860ggatgggagt cctctggggc agggagtgaa agccccggag gcagaaaggg acggagaaca 61920ggggcttgcc cagagcatgg ataggaaagg agctggggtt ctccggggct cagcgcgcac 61980tgagaacctg tgcccggggc tgcagctgcg gacgataaag gcgctgtctg gctcatgaag 62040gccacctgga tcaggcttct gaaaagagcc aagggaggaa ggctgaagaa ttctgatatc 62100tgtgtaagcg caagggcttt cgtttgtggg gagaggttat tttgtctctg cttttctgtt 62160gttgcagtga gtgcaggtag agagggtggt ttgatttggt taatcggatc atgattgcag 62220gagagggaag ctagggagat tcctctcatt ttttgagctc cttttcctag gttgtgatta 62280attgccagat gagctatccc aggcaggtgt tttctgtaga cggcttttta ttaaatctag 62340gtaaaggtat tttaaaatgt gttgctggaa gatggagtga agttagctgc tagtgtctta 62400cagttttatc agagaaaata gcaggtgtat aaaaaggtaa acaaggcttt ttcagattaa 62460agtgggaatc atgtcatggt agtgacattt tggagagttc aagtttataa ctcagacttt 62520ctttcatgtt tttgttaagc ttaatgtcct cctagtttca aatacctgct ttagcatgta 62580gattgttctt gaagctatcc ttgaaaccca tcgttgtcct tcagttctgc actgcaccat 62640caagctgttt aattataaaa ccaacttgtt ctggcttcca ggaaggatag ttttttggaa 62700tataaagctt gggaaggaat ggcaactcag tagacacaac tattctattg aatgtctgca 62760aaccagagca tggttttcta gataggttgc tgcaaaatga attattttct cctcttttca 62820ggaaagtata gaacaatccg ggaagctaac tgctgtgtcg atgagacttt tccaatgcaa 62880acatcataga ctgaatctac tttaaaatgt acattttcct ctccagggtt cggcagactc 62940ctacactagc cgtccatccg attccgatgt atctctggag gaggaccggg aggcagtgcg 63000cagagaagcg gagcggcagg cccaggcaca gttggaaaaa gcaaaggtaa aatcgtttcc 63060tccctgccaa gatctttgca agttgtgctg tgcccctgat agaccacctg tgggagtttc 63120ccctaaaggt tgtttgatct attagagaat ttcattgtct ggttttagaa aggtacaaaa 63180aatggagcct agtttcttcc cttggtagaa aaaggaagtg tggtatgtaa gactggtctt 63240ctatgttgtt ttataatcca agcaactctt ttctgtgggt ggatggctta tttggacaca 63300tacaaaagga tccttgaatt tgccaagagg aaaaactgca cctaatatca acatgtttta 63360cttcatttcc acagtaaact atatctctgt taagaaaaaa aaaaaaacca gactgacttc 63420aagctattgt ttattttctt agacttgaac agtttttccc ctgaatttta cttcataggt 63480gttttatgtt agtatcctgt attttcaaaa tggattacat tatgcttttc caaaagcaac 63540agaggatttt attgattttt aaaagtttct tctcttagaa catgttcttc tccattccat 63600cttagtaaag tgtgtatata tggaggggag actgcaaagc agctatgaaa tagatagtta 63660caggattcat ctcaggccat gtctcatttc agtcattgca gtgatttgaa ttctttctcc 63720catgctgtcc ttggaatagt ggctatttct aaagcataga ttgttcgagt tgaaggtggc 63780ttggtttgtg ggatactcag tcacttctca atgaacctat tttaacatgg ttgttgattt 63840tcctttgaaa aacttgtttt gaaaaaggct aagtgccttg ttgatcatgc tagaaatacc 63900aggatagcat ttctactgcc tgcctcatct ctgagatgag taaaatctat gtgtgttgct 63960atgggaattg tggttaggag attctggttt gaatgcatgg acagccctta tgtaatacat 64020tattcatgac ctctagtgcg gctgccatca tagtctactg aggaactgcc aggagacctt 64080gacttctaaa aagcagtact tttgtatatc agaatgatgt tcttacctcg cctggcaggg 64140aaattttctc caggactgac agtcttctct gaaaggcagt tcataaatag cagatgcagg 64200gagggttttg tgatggtgga aactgataaa actggacgtg gcccagttgc catcaatgaa 64260tctgctctac caatgttagc cacgtagaac attgtgaagt tgaaattaaa taatgaagaa 64320cattaaataa tgtatgacag tctgcattgt agttaatata ggttgcagct gagagtctga 64380attctatatt ctatattttg cattgtgtgt aaagaagagt acaaaaaaac tacaatttgt 64440attccgaaaa taagattgtt ctatttgcgg ctaatttatt catgtaagtt tcacatacca 64500aaattgaaca gtttctcggg cttatatgcc atcatgatac agcccaatag actgtaggtt 64560tggttttcaa ttgttatgta attggggcat ttttgttcat gactctgtgg ctgtgtcatg 64620agttagatga cattgctgca aagaactttt aaagtatctt ttaaccaagc atggatcgaa 64680atgttgctcc taaatcaaac cagggtcgtg ttacttgtcc tacagcctaa aaagatgata 64740gttagaaatg atgtagagga taaaagaaaa tgaagagact ttaaaacaaa ggcaaaataa 64800ttgaaaaact atttcctatg tttaaggcaa gatagtttat ttaatgggtg agatgggaat 64860ggttaatggg tacccaaaaa aaaaaaaaat agaaagaatg aataaaacct atttgatagc 64920acaatagggt gactaaagtc aataataact taattgtata tttttaaata acttaaagaa 64980tgtaattgga ctgttcgtag ctcacaggat aaatgcttga ggggatagat gccccattct 65040ccagggtatg cttatttcat attgcatgcc tgtatcaaac catcccatgt acctcataaa 65100tatacacatc tatgtaccca taaaattttt taaaaaatag tttatttata ttttttcttt 65160aaaattaggt caatatttta aacctttgat gtattcaaat gagacacact agggtaagtg 65220agaatttaaa tatgtctcaa aagaagatac aaagatgagc attgaatttt cctttatatt 65280aaaatcatgg tttggtaagt ctggtttatt aatcccaata tccttgaaga aataatgcaa 65340aatagggatt gtagaaggct agttccctct ataaattttt attgttatct ataaaaataa 65400cctcattctt tgccagaatt tcatcttgcc ttaatgaaac ctacttcaaa atgaaggctt 65460aaagtttaaa aacataacaa ttcatgctat ttaactttaa atagttaaaa acactttgca 65520cagccatttt ggaagaatca gacattttct acatttactt cgtaaccatc gaagcatctc 65580tcaaacagaa agactctaca agtcagtgct tttactcacc ttacagatca catgtaataa 65640ataagtctgt gcagatgacg ttagcagtgc tttcagatcc tggtcctgta taaatacaaa 65700cacattatcc actaattgcc taataatacc tctgtgaaat aacgtgatta gcaagaagtg 65760agactagaga tactgtcttt taaaatttaa gtgacctaca aaaggttata taacgaagta 65820ctctgtgctg tcaataaacc tattactctc cactaccact ttgttaaggc ttttcttaac 65880cactaaactg cagtgaacag attagatgtt aattttgagg ccaataaata ccaactgaca 65940tgacattgtt ttttaacgtg gtgttttctg gttccaaatg tgtgttttga tgttgtacag 66000ttatggcata ctttgcaaat cctggtcatt tcagtttgtg ctatctgata ttaccttaaa 66060gacaagtaat atatgaatgt aatttattcc cacgttgatc ttctctttga aaacaacttt 66120attgagatat aattcaccca tttaaagtgt ataattcagt ggtttttagt atgttcagag 66180gtgtacaacc atcactataa tcagttttag agcattctca ttgccccata aagaaattcc 66240aagcctgtta tcagtcactc tccattctcc ccaaccatct ccctgtcccc atgcgtagaa 66300ccactattca acttttgttt ctacaggttt gtctattctg tatattgcat ataaatgaaa 66360tcttaccata ggtgatcttt tgtgattggc tacttttgga catctgggtt gttttcacca 66420tttaggtatt gtgcgtaatg ctgttacgaa cattcctgta tatatttttt tgtgtgtggg 66480cgtatgtttt catttcacat atgtatgtac tttggagtga aattgctggg tcacttgatc 66540atctcttttt aattgaaata atgattttct tcttttctcc tttgatcctc aaaaacgatg 66600ccttctgccc tatatacagg gactctgagt tgggaaatct ggatgtagaa ggagttgaag 66660cagtgataag atagactcag ccaggcacaa tggctcatgc ctgtaatccc agcaatttgg 66720gaggccgagg cgggtggatc acctgaggtc aggagtttga gaccagcttg ggcaacatgg 66780tgaaacgctg tctctactaa aaatataaaa attagctggg tttggtggtg tatgcctgta 66840atcccagcta cttgggaggc tgaggcagga gaatcacttg acgctgggag ggaaaggttg 66900cagtgagctg agatctcttg ctctcacgtg tgcaacagag caagactctg tcaaacaaac 66960aaacaaacaa acaaacaaaa aatgatggac tcaatgtgat cttcttagcc gaatagaata 67020accttcaatc ttgagtccat tgttctttta tcatcgaata tctactatat taaagaaaat 67080gttaggccca gttggtgaat aggagagact gcaaggccgg agccacaaag aagaaattgg 67140caggtctttt gcctgcaaga agcttccatt gtttgttttg ggagaaagtt gtggtcataa 67200aaggtggaat acaataggtt gtggggaagg gagggataga gaggtctgtg agcacccagg 67260tttagactgc atcctactgg caggaagaga aaatccttct tggaggaggt agtggcttaa 67320aagtagacct cacagcaggg gttcgtattc tgttaggaac caggctacac agcaggaggt 67380gaacagcagc agccagacag gaagcttcat ctatatttac agccactccc cactgctcac 67440ataaccacct gagctccgcc tcctgtcaga tcaatggcag cattaggttc tcataggagt 67500gcaaaccctt ttgtgagcta tgcatgtgag cgatcgaggt tgcgcactcc ttatgagagt 67560ctaatgcctg acgatctgtc actgtttgcc atcacccctg gatgggacca tactagttgt 67620aggaaaacaa gctcagggtt cctactgatt ctacatgatt gtaagttgta taattgtttc 67680attatatact acaatgtaat aataacagaa ataaagtgca caatcaatgt aatgtgcttg 67740aatcatccca aaaccatccc caaaccccag tccatggaac aattgttttc cctggtgcca 67800aaaaggtgga ggactgctgc cttagaggat ggacaggtga agtttagctc ttggaagtac 67860aaagagggaa gtaaagaaga gagaaatgcc tcccaggccc tggaatacag tgtatgcgac 67920ggtcagtgta cgtaaaggct gtgcagcaag aaaacataga ccctttcaga gaaaggttag 67980tttcttgaga aaatgtcttt attctacctt cacaaattag ctggttatag tattcagaat 68040gaaaaatgtt tttctgcaga tttgtgaagg tttacttcat ggtcttctaa agtccagacc 68100tgctgttctg tctttttttt tttttttttt tttttttttt tttgagacag ggcctccctc 68160tgtcgcccag gctggagtgc agtggtgcca cctcagctca gtgcaactcc acctcccggg 68220ttcaagcgat tctcacacct cagccttctg agtagctggc attacaggca cgcaccacca 68280cacccagata atttttgtat ttttagtaga gacagggttt ctccatgttt tccaggctgg 68340tctcaaattc ctgtcctcaa gtgatccatc cgcctcggtc tcccagagtg ctaggcttac 68400agatgtggcc caccatgccc ggcctgttct gtctttaagt ttttgatttt tgtttttttt 68460aaatctctaa tattttggga actgatcttc ctttggtatt ttgacatttt gacatgacat 68520gccttggtgt gcaccttttt gtttattgta cttggcttct catgcattca ttgtgaaggg 68580ctgatcagtt cagttctgga aactgcccct ttttttggaa taatttcttc ccctatattg 68640tcctcttttt actggctctc tggtttgtgc gatatttgat ttgagcctct gtgtaatcat 68700ttttctcact ttttctccat ctttttgttg tacctgaatt tttctcaact tctaacccat 68760ctgctcaaat tatatttgtc aaaatgtcta atttccaaga gcattcttct tttcttcatt 68820tatagcttct tgtttttatt ccatagccat aatattttct cttatgtctc taaagatact 68880aatttttttg aagttacctg tgctgtcact gtttattaag cttctttaat atttgatgtt 68940actttttaca taatggagac ttttttcaaa tgtttgattt ctaatgacta tatttattat 69000ttcatatatt tgaagtgaaa ataaagtgta agaagcagca aaagcatgca acttcatgaa 69060gattatgaag tggaagcacc tattgtagag ggttaaaact gagttatcct gtaggaagtg 69120caaggactac ctacacaaag atcatcagag gtgaaattat tgactgcctt ttggaattta 69180tacatcaaca aaaagttttc aaatgaactt aaggaaacat tcactatccc tgactttttt 69240tttttttttt tttttgagat ggagtcttgt tctgtcaccc aggctggagt gcagtggtat 69300gatctcagcc cactgcagcc tcttcctccc gggttcaggc aattctcctg cctcagcctc 69360ccgagtagct gggactacag gtgtgcacca ctgcgcccag ctgatttttg tatttttagt 69420aagaccaggg gtttcaccat gttggccagg ctggtcttga actcctgacc tcaagtgatc 69480tgcccacctt ggactcttga agttctggga ttacaagcat gagccactgt gcctggccat 69540acccccaact tcttagctag ggagtatagg tgagaagaca tgagaagatg ttgaaaatct 69600cttacaaaca cataggttta caaaacacaa gcctaaaacc aagatgctca ggcagtgctg 69660aaatttgctc ttgggaggaa atcccagttt catgatgttt gaaaacattc tttctcttgc 69720tggatcacgt gtcatcagat agagaagggc caaaattgta gtataaaagg ttctttaggg 69780atgagattat ggacgcaaat tttttcttca agggaagagt attttttctt actactagtt 69840ttcttcaggt ttgtttatta ttattttttt agacaaggtc tcactctgtt gctgaggctg 69900gagttgcagt ggcatgatta ctgcttactg catcctcgag ctcctgggct caagtgatcc 69960tcccacctca gcctccagaa tagctgggac cacaggcatg cgccaccatg cctcgctaac 70020attttttaaa attttttttg taatctcagc actttgggag gccaaggcag atggatcacc 70080tgaggtcagg agttcgaggc caggctggcc aacatggtga aacccgtttc tactaaaaat 70140acaaaaaaaa tagctgggtg tggtgttgtg catctgtaag cccagctatc ccagaggctg 70200aagcaggaga atcacttgaa cccggaggcg gaggttgcag tgagccgagg tcgtgccatt 70260gcactccagc ctgggcaaca agagggaaac tctgtctcca ggaaaaaaaa aaaaaaaagt 70320agagacaaag tctccttatg ttgcccagcc tggtcttgaa ctcctgggct caagtaatcc 70380tcctgcctca acctcccaaa ctgctgggat tacagatatg aaccactgtg ccctggagtt 70440tattcattca gttttgacct gtcaaacttc tctacttcca acaagttcag cttatcactt 70500atcttgacta aaagaaagcc tgaaggcttg aagtttcggg aaaggccatc cgctgggctg 70560agactctgtc tgactctgga caatgtttaa tttttgatca tctattcata attaagagga 70620agacattgta aagctgattg gaagctccaa atgcagtggc aaagcttctt gacaggtcat 70680cttgtatagg gtaattgggg agggactgaa gaactggtag gagtgggcac cattatcttt 70740taatctggcc aatgccccag agaggactcc cccagtctcc tgtcttgctc tattggaccg 70800ctcacaggct tttggcagtt gtccaagaaa aggtggctag agtttcatca cttacgatgg 70860agatcttata attatttaat cctcctatgt tcagtatgtc actcgccttt actctcagct 70920atccttctga ttgtctgaaa tctcctggtc atcctctcca gagaataaac ctttggtctt 70980ctggaagtga ggggagagag aaagtggatt ctgataagtc caatgtccag tcagtgctgt 71040tttcagcccc agccatactt catccttcag aggtgcctgt tacctccagt tccacctcct 71100tgccagggcc ctgcagctgc aatcagcttt ctactcctta gccgtcctgc ccgcctcttg 71160caggtactct tattctaaca ctttctactc tgctagttaa ttagtgcttg ttcattgtgt 71220tctatattct atgtttccac atactctcat ctggtttcct gttcttagta tttgtgggtt 71280tatacttttt ctaaaaacat attttcagga ggaagcatta gttcagttga ccatgttttg 71340ttcccagtag ttttcagtaa acatactaca aaaaagaaaa aatatttctc atcttgcctt 71400agttcataaa tgccttacag attgagagaa ttcccctaac tagcctttga agaagaaaga 71460agaaacatga acatctttac gtcccatcag cctgaagata gtttatttca aaaaaatatt 71520aaacatgttt taaaaatatg tcttattgtg aacattatat gggaggtagg agagaaatat 71580acacatatgt ctataatgcg tatgttctta aacctacaag atagactact ggagaatcca 71640gagaataggt ggcaggtgtg aaactagagg gctacttatg gggaaaaaaa aaatgtgttg 71700gctgggcgcg gtggctcatg cctgtaatcc cagcactttg ggaggccaag gcaggtggat 71760cacaaggtca ggagtttgag agcagcctgg ccagcatggt gaaaccccat ctctactaaa 71820aatacaaaaa ttagctaggc atggttgtgg gcacctgtaa tcccaactac tctggagact 71880gaggcaagag aatcactgga acccaggagg cagaggttgc agtgagctga gatcacgcca 71940ctgcactcca gcctgggcga cagagcaaga ctccatttaa aaaaaataaa aaagaaagat 72000aaaaatccag cagcgatacc ttatgaaaaa cgcaagcaca aaatatgtca cacattctta 72060ttaaaatatt ttaggctgaa attattttca caatgtacat tttcatgctt cttgctttaa 72120aaaattgtca actctttgct tataagcatg tacctttcaa gtatagttag aaaactggct 72180gaatatgtct aatttctagc cgcatgcgtt tgtttattgt tcaaatacgc attagcttag 72240attgcgtttt tctacctgtg ataaataaaa agagtgcaga agcccgctgg acctctgtta 72300ggcttctctt ccctgttgac atatacttat gactgggtag gtaacaatca cacacattgg 72360tactgtcgtt cttccactca cctctaatgt taattgatga taatattaat aatgacaaca 72420gtaatagcac ttaatgccca atcactgctc attatagaca atttgaaaga tataaaagga 72480aaaggcaaaa tttgtgtctt tgagcatttt tttttttttt ttttttgaga tggaacctcg 72540ctctgttgcc caggctggag tgtagtgcca ccatcttggc tcactgcagc ctctgcctcc 72600tgggttcaag caattctcct gcctcagcct ccggagtagc tgggattgca ggcacctgcc 72660accatgtggg ctaatttttg tattttaagt agagatgggg ttttaccatg ttggccaggc 72720tgttctcgaa ctcctgacct caagtgatct gcctgcctcg gcctcccaaa gtgctggaat 72780tacaggcgtg agccactgtg cccagcctct ttaagcatgt agaaatatat ttgagcagtc 72840atgaccttac aatacataca gcactgatga ctacactatc agcaaaatcc ttactctcga 72900agttcttgct gcaaaacaaa cagatctcag cctagtggca ttaagtcacc attttattta 72960ggttacactt ctctgccaca catccctggc cagctttctg gttagttggt gaccaggtga 73020cctgggatgg cttgtcacat gtgtctggag gctgatagct gtcagcatgt gtgtctcgtt 73080ctcttccata tggttgttta tcctccaaca ggctggctag ggttcgcatg ctggtcccag 73140ggctgcttca tgagagagca agctccaagg gggtgataat catatctcta cttatgtcat 73200gtttgctaat gtcccattgg ccaaagcaag tcaccggcct aagcctggag tcaaatggga 73260attaccaagg gtgtggattc aggtgggata tgttgcaacc actttctgca aacaacctgc 73320cacattcatc ttttttcttc cttacccctt tggaagaaga aatatctgca tgccttgtga 73380aataaacaac gctgtcctcc cttctgtcct atttttttcc cttctttcta ctgccaacct 73440tatcacccca ggctttattc tcctgcttca atttcttccc tacttactct ttaacaccgt 73500gaaactgatt tctgtctcca tctctttact gagggtctct agtgacttcc taatcacaaa 73560gccactgacc ttttattaaa cctccccttg acatctctgc aaacttcaat ttgtttttct 73620tctctgctaa gtcacttttg tcttgcaggt gctggtacac acaaagtcta cttctactac 73680cactctggtg gttcctgttc agtctcttct gtttcgactt ttagttccct cacctgaaat 73740gtggaagtcc attcaaccaa atgctcaggc cttggcctcc tgatgtttgt tgctttaggc 73800atgtcattgg tttccagact gagctctgta gagccccaag agatccatgg attcttctgg 73860agtcactgca gaggcttagg gttgcccagc aggctgtggt ctgtgcattg tacccaggat 73920tcagccagaa caatgctacc ttcacatgtt ttattttatt gtgttgtatt tttatttttt 73980attttttgag atggagtttt actcttatta cccaggctag attgcagtgg cacgatctta 74040gctgactgca acttctgccc cccgggttca agtcgttctc ctgcctcagc cttccaagta 74100gctgggatta caggcacatg ccatcacacc caggtaattt tttgtatttt tagtagagat 74160ggggcttcac cctgttgacc aggctggtct cgaactcctg acctcaggtg atccacccac 74220ctcaacctcc caaagtgctg ggattacggg catgagccac tgtgcccagc cacacatatt 74280ttatgtattg ggacctttat gtgcattttc ctttgaatga agggtttcac ctcaggagcg 74340agctttaaac cactgccatc ttcccttcta ttctctcata cttctccagg cttcagccgt 74400cacttatagt tagtgtcttt ggtcttcgcc tctctcccta gtgcttggat ctcaggtcct 74460ctttttggat ctctgtcggt tctgccaccc acttaaaatt gagctcccag tcttttcctt 74520taaacccttt cttctctgca ttttccattt ccattctcat tacctccatc cttccaaatc 74580tgaaaccttc gtcgaagggt caacaaatct tttcctggga attatcagaa gggaaatatt 74640gcaggggcag gggtcatgca ttctctatgc aactgctcag aaacctggat ttggattcca 74700tctctgccat ggaatggctg cctgactttt tgataaatta caggtgaagc taagttttgc 74760catttgtaaa aggacactaa cctctgtttg acaggattcc agtgataatt gaataagctg 74820ttacgtataa agcacaaggt tcctggacct gtctttccct actctagccc ttgtgatggg 74880cacaggcaac tgggacacac agcacctgcc gtggagcagt tcagggtgtt gtgaggaatc 74940tgaatggaaa gtaatgaaac aagtgtcttt gcctcctctg tagttatgtg cggtcttaca 75000ctattttaca tggtcactat cagcttagtt gtttgtcact tgttggtgac tgctgtaagg 75060tagatttgag atgtccaggg actgcagagc tgcctgggat atgactggca ttgacacact 75120tgcttgatct cacagcagtt gccatggcag gtcaggttgc atccccaccc atcccagtga 75180cgaaggcatg ggccttgtct cctgtactct aactttgaga tgtgctttct gcctcttagt 75240cacatgggtg cagatctctg cctgagttat caagagcaat tgaactctct cttgctcctc 75300tcacaggctc ttttctgagt aacaccaagt acttgggaac aaaattacac attgctctct 75360gtttatttat ggttatattt atcttggcaa tcgacctgtt aagctagttt taactttctt 75420atgggtaaca gtcacatgtt ttaaaaggaa cctgatggtt ccagaatcct tgttactact 75480accttttttt tttttttttt tttttggaga cagaatctct gtcacccagg ctggagcgca 75540gtggcacaat ctcggctcac tgcaacctct gactcccgag ttcaagcaat tctcctgcct 75600cagcctccca agttgctagg attacaggtg tgcaccacca cagcctgcta atttttgtat 75660ttttagcaaa aatggggttt caccaggttg gccaggctgc tctcatagac ttctggctat 75720tttaaaacca taaatagatt aaggaaggat tattataaat tgcagttcaa gctttttttt 75780tttatcttag agcactataa ctgaatgtct ttgttgtatc attgtaaaca cagcaataaa 75840tatgcagttc ttccctccct ttttctccac cagatgttaa cagggaacga catgaagact 75900tttacgcaga gcttaaaaca gtcaaccctc tctgttctta caagtttttg ccccatcaat 75960ggctaaataa aagatgttga aaactgtgga aaaaagtaga atgtatttaa ttagcaaaca 76020tttgatgagt ggtatcatgc tatcgagttg gaatttaagg aagggaaatt gaagatgtgt 76080tagatgtggt ggtttcttcc tcaagaagct catggtctaa tggtcatgct aaaaatgtgt 76140atcactgagt aatgtggtag ctatcagact taaagcactg taggcttggc atggtggctc 76200atgcctgtaa tctcagcact tcgagaggct gaggtgggag aaaagcttta ggccaggagt 76260ttgagatcag cttgggcaac acagtgagat ctcatctcta caaaaaataa gcaaaaatag 76320ttaggtatgg tggtacatgc ctgcagttcc agctactcag aaggctgagg caggaggatt 76380gcttgagcct aggagttaga agccacaata agctataact gcaccaatat actccagcct 76440gcatgacaca gaaagacctt gtctcttaaa aaaaaaaaac aaagaaaaga aaaaaaaagc 76500attatgagcc aaatggcaaa tgggtggacc ccaacactgc tggagaaggc agtgctgatg 76560accagagaag ggcactttaa attatataag cccccactgt tagtgactga ccctctggag 76620gcagagccta cgttaaccag caatgtgatt ctctctaagg ttagcaagat gattgctaac 76680tatggggtcc aacaacgcct gtcattttag attattttca ttttaaatct caattcgcaa 76740tttacttaat ggcccaagca ttttttcatc ctcataacca ttcgtaagtg tacaattgta 76800tgaagtattt cacactgttg tgccacagat gcctagaatc ttttcatctt gcaaatctga 76860aactctatat catctgaaca attccttatc accctgccct gcctcagccc cagacaacta 76920ccatgctaca attttttttt tatttgctca aatttatggg gtagctaaga cattttttac 76980aggtatataa tgcataatga tcaagtcagg ggactcagag tgtccgtcac ccaagtacag 77040cagttttgtt gagtatagtc atcctactct gctgtcaaac actgaattga ttccttccat 77100cttactgtat gtttgtaccc tttaacccac ctctcttcct cctccctgca ccccccactc 77160acccttttca gtctgttatt tttccactct ttatctccat gtgttcaata tttgtccctt 77220tgtgactggc ttatctcagc atgatatcct caagattcat ctatgttgtc ccatgtgaca 77280gaatttcctt ctttttcaag gctgagtaat attccattac atgtatagac cacatttctt 77340tatccattta tctgtcaatg ggtatttggg ctgcttccac ctcttggcta ctgtgaataa 77400tgctataaag ttttgtttgc ttctttgttt tttgagacaa agtctcactc tgtcacccag 77460gctggagtgc attggcacga tctcagctca ctgcaacctc cacctcctgg gttcaaagga 77520ttctcctgcc tcagcctcct gagtagctgg gactacaggt gtgcgccatc atggccagct 77580aatttttttt agtagagaca gggttttgcc atattggcca gagtggtctt gaacccctga 77640cctcaggtga tccaccagcc acagcctccc gaagttttct ttaaatcttc aacttttttt 77700caatgagcct gtcaaggtgg actgtcagca agcaggaact atagcgtatg tttcacagcg 77760gctcagtgtt taacttcccg ttgtattgtt ttgcttggtc tttaacaagt actaagtagg 77820gaagtggaag cagatatagt taccccattt tatggatgag caaactgatg gtggatactg 77880aaggatcttg cccaagctca ctgagctcat aaggggcaga attagggcaa ggacccaggc 77940ttctgcctca tttttttttt tttaatgttc tttcacctca tcaaggagct gcttttagaa 78000agacacaggc ttatgaaagc agaaagtgca ttaaaaaaaa aaagacacca gctgagaatg 78060cattaatgtt tcctctttgt ttcatgctct agagagatta aggaaataaa agcttttttt 78120tttctcttta actataaaag caaaaataaa gcagggcagc tattaagaca attttaaaag 78180tataaacatc ttcgtgcaat tttttttgct gttgagtctg gatagagttt acaagcaaaa 78240aactcacctt ttgtcctgct ttttaaagtt tagtttattg tcattacacc attgatgtga 78300aacaagtcaa tttttacact tcaacattat ttaaacaaaa atcttgagct aaaaattcct 78360tttttttttt tttttttttt tttgagacga agtttcactg ttgttgctca ggctggaatg 78420cagtggcgcg atctcggctc actgcatcct ctgcctcccg ggttcaagcg attctcctgc 78480ctcagcctcc ctagtagctg ggattacggg cacacaccac cacacccggc taatttttgt 78540atttttagta gagacaggtt tcaccatgtt gaccaggctg ctctcaaact cctgacctca 78600agtgatccac ctgccttggc ctcccgaagt ggagataaaa attcttgaat ttaatatgtt 78660gttagttaaa ggaagacctt accaggatgt gctgtgtata tccttttcat ggttaaatgg 78720agatacaaat ggaacgtact taatactatt tgaatatgcc ttttcctgta gagaaaatgg 78780aaaaataatc aaatatctag gaaatagatt gtatgaagaa tgctgaagaa acaaaattta 78840aatagagcaa ggaagagaaa attgaagtta aaatgaaacg atttaaaaaa agattttatt 78900aagtgttgcc tattcgtaag gggaaaaaaa cgaaaggaac aaactaaaaa ttacagcagc 78960caaaaatata aagttagatg taacaattga ctagagaaac atgaagacga gttataaagg 79020aagattagga gactgctgta gtgcaggagt gcagggttgt ggttttttgt ttgtttgttt 79080atttgtttgt ttttgagatg gagtttcgct ctgtcgccag gctggagtgc agtggcgccc 79140tcttggctca ctgcaacctc tgcctcccat gttcaggcga ttctcctgcc tcatcctccc 79200gaggagttgg gactacaggc gcacgccact atgctcagct aatttttgta tttttagtag 79260caatggggtt tcacgatatt agccaggatg gtctcgatct cttgacctcg tgatccaccc 79320gccttactaa actccatttt taaggaagga aaatgaatcg cacaatccgc tgtcagttgt 79380ttattctgat ttcctactaa aaattatttc aattgatatt tgcaggatta acacatgtta 79440aagcaaacta aatatggcct gagaaggcct ccttacttct acatttgagt cctcgtggat 79500gaactgtaat ctagcttaat aggcagataa gattgaaaac ctaacttgga agtatgtgcc 79560tgtaacagta actgagtctt ggccaatccc agcggccgta cttcaaccac tcacagactg 79620ctaagtgttc aaactgtgtt caaataagac aaacgccaat atgtagccag tccagctatt 79680tctgtacctc actgccgatt tctgtatgtc acttcccttt tttttggtct ataaatgtgt 79740tctgaccaca gggcatcagg gcgtcagttc ttgaatattt gacaccagta tcatggtctc 79800atcctgccct ccctagactg gacaggaagt gaattcaaca ggaagtaggc aaaaatgtac 79860agtctaatct tctcgtcttt ggctttctcg tttcattccc tggattttta aacaaccaac 79920tatccctttg tcttcttaga ggaactgtaa tcatccctga atgaaatatt tattggatca 79980gttgtaagct tacatttgag tcaacctaca ttccttacag ttgagggtta agtttcaaga 80040tcctttatga ggcaactcag tgacacgcat ccccaggcag aattaaagga gatctttggc 80100tgtgacaatc taaagctctt tggctatgac agaacaagga aagttaccgt ggttgaacta 80160gatgggaaga actacttgct tctcctctga gcgtactgtg acttgcttaa ttctatgagt 80220ttaagtagcc tgaggattca ataggtcaca taatggatat gcaaaatgga gtatagaaac 80280cctttaataa gtaaaaatct ccaggtgctg tattcagcat acttgcatgg atccctgaca 80340ctgtggtgta tgttaggcta agacatagaa aatgcaatag taactgtatt ataatgtaca 80400caaccattct cttcaactct ttactttaaa atgagagaga gagaaggtga cacggagccc 80460tagtcacaca gctatgattg gactaaggtt atgaactagt gtccatttta ccacatggtc 80520catctactga attggagttt caggggtggt agttgagtca ggaccctctt attcaaatga 80580tcaaaagtcc aataaaggtg tacctggaaa aggggtcttg tccagacccc aagagcgggt 80640tcttgaatgt cacatgggaa agaattcagg gaaagtcaca gaatatattt aaaacaattt 80700attagcaact gctgtattac agagtagagc atcctcagaa agaaagggga ggaatgtccc 80760tatcttaaat ggaatgctgg cttatatggg ttattaaggt taagaatagt gtactttatt 80820acaaaagctt gtgatcagct gtgacaggct attagtattg ttattttcct atgttaatat 80880tgatttcagc aagaatttag gaatgcacta ctatctctaa agcaaaactt cttattaaac 80940taagaatgtt ttttctgttt aaaatatcag gacatttcta taagttctag gtttttattt 81000agttagttag catcattaac tcgttccctc aactgtaagt atcttatgac caggcgtgcc 81060caaccccctg ggaatgtaac ccagccagtt tggccttatc tggcctttgt tcaagatgga 81120gtcactctgg ttaggatgcc tgtgaccaaa ggacatagcc aaaacttgaa tgtttcatct 81180cagcctaata cactgatgaa tgtatactcc agcattaatc ttttgttatg aaaccaagat 81240ctattcttta agtagaagtc tgtgtattag tctgggttct ccagataaac agacctaata 81300ggagataaac acacatacac acacacacac acagagagag agagagagag agaagtatat 81360ggagatttat tataagaaat tggtgcacat gattatggaa gctaagaaat cccatgatct 81420gatctgccaa ctgtaactgg ggacccagga aggctgatgg tataattcca gctccaagag 81480cagagaaaag atcgatggat gtctcaccac aagcaggcag gcagaaagaa aaatgagtga 81540attcctcctt cttctacctt gtattctatt caagccccca acagattgga tcaagctcat 81600ccacattgga gaaggcagtc aacttactca gtccaccaat tcaaatgctt atctcacccc 81660aaacaccctc acagacatac ccagaaatat tgtttaaatt tgcaccctgt ggtgcaatca 81720agttgaccat aaaattagcc atcacagtca gctaattgaa gttattactc tacttatatg 81780accatcttca gtctagaatt tagttaaaca ttcgagttgg accattatct gtaggaattt 81840gggttccagg aaatacaaga agaactgaaa ataatttttt aaaaaaattt aaaccatagt 81900catgtttatg cctgaaaata atactttaaa ttttttccta aatattctag aaattaaagt 81960ttaccaaaca tgaaccattt gttattaata ttatactttt gatgaatgac ttgataaaat 82020acaaaagaag gaaatgtatt ttttaggaga ggaatttgaa taatttttcc ccaaaaaatt 82080catatgtatg taaaatatct tcatcttagt aaaggtccat tagcccttgt taactctggt 82140ccaagaaaat ctatcaattc aagttagaaa aacaggtact caataatctt agcccttgac 82200atttttcttt aaatttctct gaaatacata tttttttttc tgtttccttg gctgaaaaat 82260gttcattgta atattaccag tgtggttttt tttgtttttt tgtttttttt tttctaagac 82320agaatttcac tcttgttgcc caggctggag tacaatggtg caatcttggc cccctgcaac 82380ctctgcctcc caatttcaag tgattctctg gcctcagcct ccggagtagc tgggattaca 82440ggaatgcacc accacacccg gctaattttt tgtatttttt agtagagaca gagtttctcc 82500atgttggtca ggctggtctc aaactcccga cctcaggtga tccacccacc tcaacctccc 82560aaagttctgg gattacaggc ctgagccacc gtgcctggcc cagtatggta tttttaaaag 82620gttttttaag tggttatttt tttctggctc atgaatatct ttgaatatat gatatgaatc 82680tgtgagcctt ctccaaaaaa tagcttatat ctaaacagct tggcttatat ttccagggtc 82740tccacaggcc tctgaaacaa acctatatac cctaagggta tacaaagtac aagaatctct 82800tgctaataag cccagccttt ttccttttag agtggctgat gcttgaagaa ctatggaacg 82860ttggggtgta ttcctttgct ctcagatact tgaggagatc accacataaa ttcttagagt 82920tctgggttta gctgtagtgt ttagtctagt gtcgaagcag aggttgggca aatatacact 82980actgtaaata ctttcttgtg ctatagctgt taagcatgtt ctagtttaaa cagttgtcag 83040tgattagcga ttttgatgtt ccgttttaag attttgtaat ggtctagacg aacatgaaca 83100aaaccaaaag accgtttcaa gttcagcaga tagtaggagc taatcttttc tgtggtttgg 83160tttggtcgcc tgccttaggt tggtttacta gtctgtgact ttctgagttg ctttatcttg 83220tgctaacttc tatcagatac aatctccata ttgattcctg tttattcttg cctggatgat 83280ctattccatt tagatagcta gaacattcag tagctctctt gagggaatcc cacctgtggg 83340ttgcagaagg gtaacctaca gatgcccgat tgtccttttt ccctggatta aaaagaaacc 83400atttgccatt tttctggctt cttagaaaaa taaatgatat atgaaatgtt tgcagcagca 83460gaactttttc ctgcccaaag gtttaagatt ccatggacac atctcctggt ctagcatgct 83520attgctcttt ctcatcaact ttccatctct cctcactcgt cacctcccac cccaaataac 83580ttgtttgtgt attcctctta cagccttttt cacagcatat tgggttattt gtttctcttg 83640ccaaacatgt acaatttggc gtacctggga ctgaggaatg gcctgttgac acttcaaaaa 83700aaattttttt taagatggag tttcattctt gtcacccagg ctgtagtatg agtgcaatgg 83760cggatctcag ctcactgcaa cctccacctc ccacgttcaa gtgattcccc tgcttcagcc 83820tcccaagtag ctgcgattat aggcatgcga caccacaccc agctaatttt gtatttttag 83880tagagacggg gtttcactat gttggtcaag ctggtctcaa actcctgacc tcaggtgatc 83940cacccatgtt ggcttcccaa attgctgtga ttacaggcgt gagccactgc gcctggccaa 84000cacttaaaat ttaatttctt tgtagaggca taataaatat gtgtggactg aatgaatcac 84060atgatctgag tgataggaag tgtgtgaatg gaatcctaaa cgtttggagg tcatcatatt 84120ttgccatctt tatcctttat cttacctgtt ttacctgttt tatggggtta tgtcttgtct 84180ctacaattac atgtgacctc agtgaggaat caggattgtc aattatgtct ccattcccag 84240tgtagtgagt gatatgtaga atgacttttg gttgcattat gacactcttt agtgaaaagt 84300aatgattaaa tagtttgggg tggagataac atgaagagat ctttttagaa actttctgtt 84360tggtgcagta ggttaccaag gcatgggccg gaggaagata tagtaggagg tggtctctgc 84420caggagaaag aattccccct gtaggaagcc aagttcctga ctagcagttg ccaaggcttt 84480ctgtgtcctg ccataagcaa cggtcccttc aagcccagtt cctgcaaagc tgttctgtta 84540cataaagatg aaattataaa tacactgggg tatctttcaa gtaaaattaa ctctatatat 84600taaaattcac cttatatgta ttccacaagc aaatgtcctg aattcttggt ccatgtgaag 84660tttgattatt gcaattttgg gaataattag ggaagagaat atatagtaac ataaaggact 84720caagcataag acatacaaat gtctaatatt cccccctcca tgtgcagact attggattat 84780tccatttgct tttgtaatat gggaatacag ctgaagaata aaatgcaatg acatgacctc 84840atgattactg gaaagagttt tttctcttat ttactgataa atctgctata atttgagatc 84900aatatgtaat aacatttttc tgcaggtgtt gactacaaat atatccctat ataataaaaa 84960tttattgaaa ataggatgtc atcttgtctt ctcaattttt tttgaaaaat taaataatat 85020ataagagtat ttattatagt gggataaaaa gaaaaaaaac tcatgtaccc acctccacaa 85080ttacagatac agagtgtcac taatagcttt gaaagctttc atgttatcct tccccaggct 85140accctgattc agagcagaga tgccccaaag tggagcttag cccactgagt tcttggattt 85200gcccaggaaa gaattcaagg gcaagccaga gggagaggaa aacagcttta ctgaagaagt 85260gttacagctc ctctactgcc cctgcagagc aaggctaccc tgtaggcaga gagtaacagc 85320tgaggggagt tttgcagtcg tattcatacg cactttcaat tgcatgcaga ttaaggggct 85380attcgtgcag aaatctctaa gggagaagta gtaatcattg ggtcattgcc acggaaaggt 85440gtggtaacgc ctgggtgttg ccatggcaat ggtaaattga catggcacac tggtgggcgt 85500gtctgattga aagctgcttt cccctgggcc ctgttttaac tagtcctcag tctggtcggg 85560tgtccgagac ccccacctcc tacctcaacc ccctctatca gagtttatca ttatgctgaa 85620tgtggtgttt cccactccct tcctgttcat aacattatgt cttaccatct atatttattt 85680tctacatgac atactggtta gtttttctgt gttcaaacca ttatttaagt agaataatca 85740aggtgttctt ttgagactgc ttttttggct caacattaaa tgtgtaaaat tcattcttgt 85800tgatttgtat caccataatt tctttttacc aatatgtggt cttccagtag gggatgtggc 85860acaatcaatc tgttctctgt ccatgggcat ttaaggaatt gcagataatc ccagtctgga 85920agcatttatc cgtgtctcct gatgcatgtg tgcaagagtt tctcttgggt atgttaatta 85980gcagtgaaat tgtaggttga taaagtatgt gtatctttac ctttactggg cagtttttaa 86040agtgtttgcc ttcatttaca ctcatactat cactgtagga gagttttcac tccaccatta 86100ctttcattga atacttttca tatattaacc ttttgttttc ttctgtgaaa tgacagtgca 86160tggctttgga cttcttttta attattaaat tgtatttttc tataaacgta caagaggact 86220ttctatattt tatatgtcat tatttttgtt ggttatttgt attgctgaaa gggtttaatt 86280tgtagctgct cttgtcacat ttatatagca ttcttaatac caatcaagtc cgatgtatct 86340atcttttctg tcactatttt tgcttttggg ctcttgttta agaaattctt tcctggccag 86400gtgtggtggc tcaatcctgt aaccccaata ctttgggagg ccaaggcagg aggactgctt 86460gagctgagga atttcaaacc agcccggaca atctaacagt acttcgttac tacaaataat 86520tgaaatatta gctggccatg gtgacagaca cctctggtcc cagctacttg ggaggctgag 86580ttgggggggt tgcttgagcc caggaggtca aggctgcagt gagccgacat tggaccactg 86640cactccagcc tagacaacag agcaagacca tgtctcaaaa caaaacaaaa caaaaagcaa 86700aaaaggaaat tctttcctac cttagcctca taaagaattc ttctatcttt atttctaaca 86760gttttaaaat ttcatctttc atctttaatt cctcgatctt tctggaactg gcttttgtat 86820atggagtgag ataggaatct gacttcatgt agaatacctg tttgttccag agctattttt 86880tgaatggctt cttatctccc cttggtctga aatgcctgct cagtcataaa ccaattttcc 86940acatatgagt aggtctgtat ctgagctgct attcatgttt caatgccata ctacctgaat 87000ttctgtagtt ttaaaataaa gattgataga gtttggacat atcaacccat catatttttc 87060aggattgaac tgactattct tggacctttc tgttccatat aaattttaga taccatatat 87120gaagtccaac aaaaaaagaa aaagaaaaaa cacaaacata ccctgctgac attttgtttg 87180gggttgcatt taagtctgta gatcaacttg gaggaaaatg cctttgttat aatattgagt 87240catcttatcc ataaatagga actatctctc cattaattta gctgttgaat gtcttagaat 87300attttcgata aaggtcatat attttttaga tttgtttcca gatatgttaa ttttttagtt 87360actacagtct ctctcacctt tctctctcaa gttacagttt ctgttggcta atatggagaa 87420attcagttga ttttgtctga aaagctttgt atttatcagt ctttctaaac ttattaaatg 87480cttatgaatt ttctgtagat tcttgggttt tctatcagac atagatagga cagtcatagc 87540atgtgaataa cggaaattct atttctggtt gagcacagtg tctcacacct gtaattctag 87600caattttgga ggccaaggta ggaggatcac ctgaggccag gagttcaaga ctagcctggg 87660caacctggcg agaatgtgtc tataaaaaaa ttaaaaataa attaaaaaat aaaattctgt 87720ttcttccact ccagtccttt taacttttca attatttttc atcttaccgt actggctaga 87780ctcctcaccg caacattagg ttattcttgt cttagtggta ctctttaaaa gcatgtattt 87840gatttgggtt ttctatagag gtactttagc atgttaagga agttttcttc tatttctagt 87900ttgctgtttt tttcttaaaa attatgaatt ggtactgaat tttatcaaat gtcccttttc 87960tacatttttt tttttttttt tgagactgag ttttgtactt gttgcccagg tgggagtgca 88020gtggtgcgat ctcggctcac ggcaacctcc gcctcccggg ttcaagcgat tctcctgcct 88080tagcctccct agtagctggg attacaggca tgcgccacca cgcccggcta attttgtatt 88140tttagtagac acagtgtttc tccatgctgg tcaggctggt ctcgaactcc caacctcagg 88200tgatccacct gcgtcccttt tctatatctt taaaggtggt catatgattt ttttgttgaa 88260tttcttattg cggcaaattt tattaataga ttttctacat ggaactgttt acttggataa 88320acaacatttt catgatgttt ttaacatcac ctttttttaa catgtcgctg gatttgactc 88380ttgaagattt tgggggaggg tgggggactt actgtatgta taatcatgag ggaaactggc 88440ctctaatttt cctttcttat cctttcctca agcgatttga cactgaagct tactgctgtc 88500ttaaagtttt tgtttgtttc tttgttttga tctctcttct tgggaagagt ttgttatgtt 88560ctgattatgt attccttgag tattttctat aactcactgg gaaaattcct aaacgtactg 88620tgttttttgt gctttgggaa gatttgacta ttgattcaaa ttctttaaca gaacccttta 88680gggttttcac tctcttctca tgccagtttg ggcattatag cttcctagtt ctttttctat 88740ctcatctaga ttttcagaat tttaggcatg aatttataat ctcattttct tactaatctc 88800tgcagtatct acagttacat cttttccttc tctcaatttt tactcaataa atctcactat 88860atatttttga ataattctat tttctatttc attaacttct cttcttttta aatttggttc 88920taatttcttt ttcatttaag tcagttactt agctcattat ttctcaaagt atcttctgtt 88980aatcacacaa gctttttaca tttctttttg agaacctggt tttctgtatc tcacaaaatt 89040tcatatgtag tatttcatta ttgttcagtt ctaagtattt tctattttca ttttgatttt 89100tttattggga tggtgagtta tctataagtg tgctaattcc caaagatatg caggtgatcc 89160tactgttgtc atttctgtct ctgatttcag gtttgtacat ttcaaatggg agagagctct 89220tttttttatt tttttagttt agtgggacaa tatttatctt tttaaaatgt agcatttact 89280cgtcttactt tggttgtgat tacacatgtt tgcacttagt ttccactaca atcttatttt 89340gtcctttttg catgctgcag tttttcttca tcacccctta tctccctatt tctttcttta 89400agaattactt tctggccagg catggtggct cacgcctgta atcccagcac tttgggaggc 89460caaggcgggc agatcacttg aggcctggag gttgagacca gcttggccaa catggtgaaa 89520ccccttctct acaaaaatag aaaaattagc cagacgtggt ggcggacacc tgtaatccca 89580gctactctgg aggctgaggt atgagaatcg cttgaattca ggaggcagag tttgtagtga 89640gcccagatca caccactgca cttcagcttg ggtgacaaag cgagacactg tcaaaaaaaa 89700aaaaggaatg attttattta tttattttaa tcaggttttt accctttatt ggtttggaag 89760ttacacatta tagttctggg cttgtttaaa tggatacctt agaaatttca gcatgcattc 89820ttaaaatcta aagttaacca gttaaggact ttggagcatt taaggctgat aactcctact 89880tgatttacat ttttgccaat aatttggttt tatgttttct taaccttaca caccaaatat 89940ttttaccatt taacttttaa aagtaagtct gtatttgttt acatttattc acttaattac 90000tactgccttt tctaaccatt tctttcacat atcaaagatt ccttttgata tcactgttgt 90060tcttgaagct tgtcattgac aagtttcttt catgagaaga tttttgacaa tgaagtctct 90120ttatgtgtct gaaggaaact tatttataga agatagtttt gttgaaaatg attgtaggtt 90180tatacttttt cctggaactt gaaagatggt atttgattga cagttggctt ctgactgttg 90240aggagtcaac tctcattata accatgtcag ctctttgtat gcagtctctc ttcctaaatc 90300ctttcaagtc ttcttctctg tctttggtgt ggtgtcatct cactgtgatc catccaggta 90360ctggtttatg tttcactttt tcctgcttcg gattcattgt gcctcctgaa tatgaggatt 90420tgagaatttt gtcaaccctg gaaaaccctc aaccattagc tcttaaaagt tgaaaagtaa 90480atgaagtcat ccagcattgt taggtattct tcagtggaat gtttgttcaa aatgtcaagt 90540taggccaggc atggtgactc atacctgtaa tcctggcact ttgggaggcc aaggcggggg 90600tatcacttgt gcccaggagt tcaagaccag cttgggcaac atagcaagac tccgtctcta 90660ccaaaaaaaa aaaaaaaaaa caaattaacc aagaatgatg ttgtacacct gtatcccagg 90720tactcaggaa gctggggtgg gaggatcact tgagcccagg agttcaagac cagcttgggc 90780aacatagcaa gactccgtct ctaccgaaaa aaaaaaaaaa aaaaacaaat taaccaagca 90840tgatgttgta cacctgtatc ccaggtactc aggaggctgg ggtgggaaga tcacttgagc 90900ccaggagttc aaggctgcaa tgagctgtga tcgcgccact gcactctact gtgggtgaca 90960cagaaagacc ctgtctcaaa aaacaaaaca aaaaaagtca acttagctcc attgccagaa 91020ttcgcagata catgaatgag gttttacccc acattatttt atctgaattt tggtataatc 91080ttctatttat tttacaatta tttacttaag cagaaaatca agaagatgtt aaaaaaaaaa 91140aagggacgta ttacctaaaa tactagggaa gaagaaaccg tgttttttaa gtagaatagg 91200ccaaatgtca acagacatcc agggtactag aaaatattga gacgcgtcta aacacacaaa 91260cctgtcttcg aacctctagt cacacagcat gtttgcattc tatatttcca tgaaacttgg 91320agtgaggagg gagagaaaat acatttattt ttatcctctt tagttaaaat tgcatatcag 91380gatttctttt tttaggagtt gcaggttaac ttctaaagtt atctcccacc tgcactacat 91440agggagaccc tgtctcttaa aaaaaaaaaa ttaattaatt aactgggtgt ggtggtggag 91500gcctatagtc ccaggtatgt gggaggctga ggtgggagga tccctggagc ccaggaggtt 91560gaggcagcag tgagccgtga ttgcgccact gcactccagc ctgggcaaca gagtgagacc 91620ttgtctcaat cgatcagtca atcagtagta aagttatctc attacagttc aatgtctcaa 91680cataggactg ggtagaagga agccttctgt cctgtgatct gtgttgctct gatacattcc 91740taaaaagaaa ctattaagag gggtagatat aattctaaca tgcaaatgtg ttgagggaaa 91800ggtgaaaagt acatattact tgattactat ttattcacat ggaaaccatt tttgtatttg 91860cagttagtgt caactgaagg gattaagaag ataatctcta tcatttatat ttgggtctgg 91920tcagatgctt ttgtaattat atttctattt gatgcctcct ttatctgaga ttttatatat 91980ttttttaaat ctggagacag gatctcactc tgtcacccag gttggagtgc agtggcatga 92040tcatatagta gctcactgta atctcagcct cctgggttca agcaatcctc tcacctcagt 92100ctccagggta gctgagatac aggagtgtac tactacatct ggctaatttt taaatgtttt 92160tgtagcgatg gggtctcggt gtgttgcaca gcctcgtctc aaactcctgg gcaagtgatc 92220ctcctgccat ggcctcccaa agtgctggga ttttaggcgt gaaccaccat tcctggcctg 92280tctgagattt tataatactt aaaacaacca cacagtgttg gctcatttaa cttcttcatc 92340cattcacatt tgcttgtggg tccagaatga atctgctttt ctactgatgc ctcaagtagg 92400tcccaacttc ttttataaat atgtaaaaat ctattgcctt ttcctgtggt tactgcttgt 92460gagagaaaac atactcgatt tatacctaga atgaacttca gcaaagttag tgaaaatgtc 92520aacataaaca caggctttac ctctgaaagt ctatgatatg aaacatcaag agctgatcag 92580gctttttccc tttaagagct ttacatataa attacaatat tttcctcctt aaaaaagcca 92640aaatgaggaa gttaacggag atgaaattac agtccactca tttgataaaa aagccaagaa 92700acaaaatgga taagcatgga taagccccaa agatatcttt gttgacctca ttgaatctca 92760tttctttttc tttttttttg agatggagtc tcgctctgtt gcctaggctg gagtgcagtg 92820gcgtgatctc agcttactac aacctctgcc tcctgggttc aagcaatttt tctgactcag 92880cctcctgagt agctggaact gcaggcgtgt gccgctatac ccagataatt tttgtatttt 92940tagtacaaat agggtttcac catattggcc aggctggtct cgaactcctg acctcgtgat 93000ccgcctgcct tagtctccca aagagctggg attacaggca tgagccaccg cacccggccc 93060aagactatta tttatgtttg taaagaaata tcaattaatt ttagaaatag agatggtgct 93120tccaagtctt aggggggaag aaaagctctg ttttaattat ccaaaatgtc aagctattca 93180ctaagatttt tcccttgact tgtagctagc ctcagtttaa acaaatacca catatattct 93240atttgaagta agatttaaaa tgttaaatgt ggcaacaaat ctgtcaccca aaagcttctt 93300cacagtcttt gataatcctt ccctcctgcc ccttctgatg tcctgatctg ctcataatct 93360tggttgtggt aatggtttca cagataatca cataagttaa aacttaacac aaattgcact 93420ctgtggaggg atggatgtgt gtgtctctgt gtgtgtgtgt gtgtgtgtgt gtgtgtccct 93480gtccctatac ctcaatacta ctgttaaaaa caaaaatata aaaatacatt ttcaaaaaca 93540ttccaggctg ggtgcagtga cttacacctg tattcccagc actttgggag gccagaggca 93600agcagattgc ttgagctcag gagttccaga ccagcctggg caacatggta aaaccttgtc 93660tctacaaaaa aaatacaaaa actagcccag catggtggag tgtacctgta gtcccagcta 93720cttgggaggc taaggcggga ggatggcttg agccaggagg cagaggttgc aatgagccaa 93780gactgtgcca ttgtactcta ccagggtgac agagtcagcc ctgtctaaaa aaacaaacaa 93840acaaacaaac aaacaaacaa acaaaaaact gtagtaaaaa gaaattcatt gatgttgtat 93900ttcaaagcaa taggaaatat agccaaagaa aatattttaa cacctttgag ataaaagaga 93960gatagagatg actggtgtca gctatctttt atgactgccc aaatatcgct tccctttggg 94020cttcctcttt ctgtgtttac attttttttt accttattag aaagtaactg tttagataac 94080actatttatt cattaaaata aatatatgta tatgcgtgta ttgatatata tatatatata 94140tatttgaaat cctattaagt acaaggcagt atgccaatgc cacatgcaga tagatatagc 94200aagatgatca agatagagta tttcatgcaa ggggaccggc atattcccaa gagcaggaga 94260gagagcaaga ttggttttag aaatggcaag cagtttaata taactggatc agcgaccaca 94320aaaaaaccag tttctctttt ataaagccag gctactttac agcatttctc ataattcaat 94380tttagtggag accaggtgat ggattaagca tccagttttg ccctgcacag aactctgttg 94440attgtattgc tgttgttatc caagaccaag cacttaggaa aaatagctga tgtttctgca 94500actcagaagc tttgtatgag ttgccttggt ttattataga gtgttaagga ggtgtaataa 94560tctgtgctgg taatagctgg tcttggtaaa gaaaagccct ttctcatgca aatactagat 94620gtaatgaaat gatgggtctg tgagtgatat ttaaggttta taactatatt tttttcttaa 94680gctagtcaag tacagtgttg agaaggagga aagagtggaa caaggagtcc aatctgtaac 94740ttgtagctac attacaaaaa taagaattat gtaaatactt atagggaaca gtaaacgtgt 94800tatggcaagt ttgaaactgt ctatccccta atcctttttt ctaccatcaa aactattgtc 94860ttaaatcaga ttttgttgtt gttgttgttg ttgttgttgt tgttgttgtt gttttaatag 94920cttggaactt cttggaacac aattgtgtta gaacatactg tgctacagta ttcctatcca 94980ttccagcaag agcttcggta gagttgtata taaagcttta ccagttaatt ttgagctttc 95040tctgaaaagc atatggcatg ttttatacag aaaaaccatt ttaaaacata gaacattggg 95100aattttggcc atgaactgaa agaaaatggt ttgaagatat gatcttctaa ttcgttatag 95160gagtggggaa gaggtttctc atatggtatg attttaaaga tctattaaaa acaaatttat 95220attatagagt tcatatataa atcatgacct ctattcatga tttagtcagg aagacagaaa 95280gcacttcaaa tatttcaggg ataaagtgat ttaacatagg gaattaggaa tgtacaaaat 95340ctgaaaggac tgaaggaata aagtcagaga aacctgccca gttctcagtg tagcccagta 95400gaatttgggg aatcaggaag tttcccagaa ttactaaaaa tcattgctga taattgcagc 95460tacctaaggc attggttggg tgattttcag gacacaacct ggaatagcat ttcccaaatg 95520ttttagtctc gtgaccactt tacattctta aaaattaata tgatgatgat tattatattt 95580tttatagaga cagggtcttg ctaggttgcc taggctggtc tcgaacttct gggctcaaac 95640aatccaccca ccttggcctc ccaaagtgct gaggttatag gcatgagtca ttgtgcctgg 95700ccctaaaaaa attattgaac acctcagtga gcttttgctt gtatagctta tgcctaatgg 95760tgttttttgc attagaaatt aaaacagaaa aaaataaatt acaagaataa ggaagcaggc 95820actcccctag ctatcagagt gataaagtca tcacaggtcg tgtaagtatt tgaaaaattc 95880caatctacca tcatgagaga acgagaatat tagaaggcaa atgatgtctt agtgccatta 95940agaaaatagt tttgaccttg tggaatccct aaaagggtct ctagagcttc taggggttcc 96000tgagccacac tttgagaact gctggcctaa tggaactgga aagtctctgg tctgctggag 96060cccacgtaga tgccttctgc tgatggtggc ttctcctctg ccttctgcat gtcacacaag 96120gataatctgg cttctcctct gccttctgcg tgtcacacaa gtggctctca ttgcaaggag 96180tccaacctgg aaccctgttg gcagagaatc tgaaaatatc atccgtagga attcatgctc 96240tttgttagat gggaaagtat aaggcaggcg gaggcatagt gccgagctga caataggcaa 96300tccaacatat ttcgttaaaa accgtgaaaa tccacctgag ttgacaatag gtggcatcta 96360catgaacaga aaaatttctt gagagtaggg tcttcacttt tcctgaaact ccctactcct 96420ttccaatgat cacttctttc cctcagtctt tgacagtaat aagaccatga aataactacc 96480aaatcaatct ttgactgctt tcccctgcat accttttttg atatagggtc tcattctgtc 96540acccaggctg aagtgcagtg gggtgaacat agctcactgc agcctcgacc tcccaggctc 96600aagcgatcct ccctcctcag cctcccaagt agctgagact acaggcacac actaccatat 96660ccagataatt tttgtatttt tttgtaggga tggggtttcg ccatgttgcc caggctggtc 96720tcaaactccc aggctcaagg gatccatccg ccttggcctc ccaaagtgct gggaatacaa 96780gcatgagcca ctccacctgc cccccaacca acccaaaatt tttaataggt ttgtttatcc 96840tgaaactttg ttttatgtgt gcacctatat gtaaaatcac tcatcaaaat tattgagatc 96900tacacagagc tcaaccaatt ctgatcctct ttggagggcc cagagcatct gtgagcccag 96960aacacagcgg ttttgcataa atgagctttt gaaagtacga tgactttaag tactctaaaa 97020tgcttagatc atttttagag aattgataag tttaacaaaa ggagaacttc agaatgaaaa 97080ttattattcc accttctatg aggaatgttt taaaagataa aagttgttgg ctattacctt 97140gaaaatctaa agctatgtgc agtaactcac aaatagtgaa ttaattgatt taaaatttat 97200ggtcattaat gatgagtttg ccagtaagag tttttcccct ctttggtgga gaagtttaga 97260aacttctgtt tcatttcaac tttgaacaaa atcctagaag ccatttcaag tatactttgc 97320taacccagaa attttggcaa gaaagtataa acttgagcct tctcggatag tctgttcatg 97380gagaacaaag cagagaatga tagcaaaaac aaaaatctaa tcaaccaatc agaacaatgc 97440tacttctttt ctattaatca ttgccacatt tctcttccct gtcctccatc aatagatcaa 97500aattagaata ggacttgtag gtaaaaattg gcccttgaga aggtatgcgt ggattacttt 97560tatttgactt acatgtggct ttatagtcag aagtactgtt gcttgcaaag catatttgtt 97620tgtttgtttg ttttttaact caaaagcacc tgaaaaaata atcagaggtt tttataccta 97680caaaatgtta ctgggttccc attccatgta gatgccaaag ccaaatgagc ttatacaact 97740gaacaaaaat aaatcatgtt tattttttcc atatgcactt agagaatatg caaaaataaa 97800tgtccagaaa taaaaatttg ccaaagattg ccacatatgc ctgaatataa gatgaggctt 97860ttttcccctc agaattattc ttcagaaaag agagagtcac cttatatttg aaatctcaca 97920agatctccca tggtgcaaga agtacatttt ccattacttt attttgaaca acaatgtgct 97980gcttgctgga gatacatggg cctgcatgat gtcaatcaat cttattttgg tgtgtttata 98040taagtcacct gatacatttg acttaaatgt ttaaatagag atttaacatt tacttctaga 98100gacatgacgt cacacttgta aatattggat gctatggtaa actcaaacat tcaaaacaaa 98160tactgtttcc aattagttga aaggaagttc ccattagaaa ggggttaggt gacccagtat 98220ctctcctgat atccacaaga cagatgagaa gttttcagag gattttttaa aaattaaata 98280tggataagga tttggagaga gggatacttc taagttagtg cagttggcca ttgaacaatg 98340agaggttatg ggcatggacc ccctacaaag ccaaaatcca tgtgtaactt ttgactcctc 98400aaaaaaccaa atagcgtatc gttgacccaa agctttacca atatcataaa aggttggtta 98460acacatattt tgtgtgttat atactgcata ctgtctccac agaagagaga aaacatgaga 98520agatcacaaa ggtccttttg ttgccaggct ggaaaatggc aggcatcatt ctccacgcat 98580ttcactggcc aaaattcaat cacgtgggcc aatctaactg caaggaaggc tagaaattta 98640gttgtctgta ttctgaggaa gaaaatggag ttagtaagca tcttggatct ctgccacagt 98700atctgactat ctttgacagc actgagagtc agccacaagg tatttattct ttgttaaatg 98760tgacatcacc ttatatttgg aatctccttc tatttgggca tatatagcag ttgcctcaaa 98820acttcgaagt catagaaaat tatttatggc tagatctttt aaagagtttt tgtaatcgag 98880gcaaattgac atagaacatg gaaatgtatc taaaatgatt ggaaacttat tataattaga 98940aatgtattaa aactcagggg aatggccctg agtctggggg tgtcgggata cctggcccag 99000ttggattttt tttttttttt tttttttttt tttggagatg aagtctcgct ctgtcaccca 99060ggctggaatg cagtggcgct atctcggttt actgcaagct ccgcctcccg ggttcatgcc 99120attctcctgc ctcagcctcc tgagtagctg ggactacagc cgcccgccag cacacctggc 99180gatttttttt tttttttttt tttgtatttt tagtagagac ggggtttcac cgcactagcc 99240aggatggtct ccatctcctg acctcgtggt ccgcccgcct cggcctccca aagggctggg 99300attacaggcg tgagccaccg cgccctgccc ccagttggat ttttatttcc aaccactgct 99360aaagcattcc tatgccattt gctctgaacc ctggactctc aggaactggg ttggccaatc 99420accgatttaa tccatcttgc tgtgatggag agaacttatg cccaaaattg ttgattggca 99480gtcaaggccc aacagccaat gggcggtagc tgcagcctcg cggtccctca ccacctgtgc 99540tctgctcgtg tttaccctgc gtgccgccaa tagtgattca agccagttat ccatctgttc 99600tcccacacca catcttccca aaaaggccgt ttcttctcct cttggctgga agcctgtgcc 99660ctctgctcac agttctgtta gagcattcgg atttatatag aacgaaggtt tgctttgctt 99720taaaacatca attccttgat cccagttctg ttctccctgg gccttatgga gtatacgatc 99780accttcttcc tctttagatt ttttcttctc tgggctaaaa atctcaaagt cctcatgtga 99840catgttttta tgccctctta cctgatttcc tcccatgtga gtgtgctata aatacagctc 99900ttccaaagca gaacacatca gattaaatac ttgtgatctg gcagggcacg gaatagaatg 99960gcgtaggcca gcacgtgaat tggtggtaaa ctgaaggtca tcggggagaa aataacttct 100020ctaccctctt cggtgtagta tttaaggcca gtgaattaaa ctaacaaaag acacatctca 100080caggatacga gacatgcaat tttattaaca ttttacatgc actggagttc acagaaaagg 100140agtgaaagtc gaagtgggag gattgggggc ttatatacca ttttaaacaa agaaaagggt 100200gtctgcattt cacgagatgc tgaattgcag gaaggtgact aggaagaata tgcggaagct 100260aatggaagat gagggttatt ttagtaaggt gtgtttctgc atctcatctc catgccaact 100320caccatctcc agcagtaaga cacaactttt ctgttcctgg tacagcagag gggaatttta 100380caagggaaaa tttgagtcct ggttttaggt agatgagggg aaggcagaga atttctcctg 100440catttgtaga ttctcagcca ccttcagttc aatataatcc ttatggcaag gtcgcatatt 100500ttggggatga cctcttctga tccccttcaa ggtacaattt gaaaccttct ccaagtattc 100560tgaattgctt tcctctggag acattaattt ccccacctcc cgctatcacc tttgaaatca 100620tgctctaagt aacccttcat attatcacac tgtaaattgg gatgtttaaa gtttggttgt 100680ttatgcagtt ataaatcagc ttaattattt gtcattcagc ttctaatagc ttctctgcaa 100740gaataagaaa gctcatctga tactcggctg aaatccagat tcgctcatcc ttcctccctc 100800cctcccttct gcctactgca tgccagtgat ctcccagcca ccaggaacag agtaaacaaa 100860acaaagcccc tgccgtcagt aggattacag tctagctcgg agaaataggc cacatgcaaa 100920tatatatgta gataatgtgt tgtagcagta agtgctaaaa aatagtctct gttctgctgc 100980tataatattt tcacctgagt gatttataaa caataggaat ttattgatca cagttctgga 101040ggcagagaag tctaagatca aggcaccggt agattcagtg tctggggaag gtttgatctc 101100tgcttcacat gtggcctctt gttgctgtgt cttcatgtgg gagagggagt ggaagggcaa 101160aaaaggatgg acaggctcct tcaatccctt ttataaaggc ataatcttat tcatgggggc 101220cccacactca tgacctaact accttgttaa agccccacct gctgataact atagccttgg 101280agattaagtt tcaacatatg aattttgggg gaacatgttc agtccatagt aaaaactaag 101340aaggtaaaga ggatgaggag ttggggagtg ataaaaatgg cttctgtggg ccaagcgcag 101400tggctcacac cagtaatccc agcatttgaa gtgtctgagg cgggaggatt gccagagctc 101460aggagttgga gaccagattg ggcaacatag tgtgaccccc tcatggcaaa aaatcaaaaa 101520ttaactggca tggtggtcca catctgtggt gccagctact cgggaggatc acttagaggt 101580tgcagtgagt tgtgatcatg ccactgcact ccagcctggg tgacagagca aggccctatc 101640ccccaccccc cagagaaaag cttctgtgaa ggtgacattt gatcaaaatt gtgaatggag 101700tgagaatgtg tccatgtgga tatttgagga tagattattt caggcannnn nnnnnnnnnn 101760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 102300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca gtgagctgat atcgcgtcac 102360tgcactctag tctgccgaca gagcaagatt ccatctcaaa aaaataaata accatccaaa 102420aactgaacta tatggcttcc ttgatggggt ttgtttttca tcaacttttg ctggctccta 102480gcaattctgc ttttcatctt tttctttttg ttactacaaa atttcctttt ttcttttttt 102540tgctggcttt actgaggtat aattgacaaa taaaaattgt atatacttat gatacatagt 102600gtgtgtggtt ttctgggatt tttgttgttg ctttttaaat tttttttaaa gacagggtct 102660cactgtcgcc caggctggaa tgcaatggca cagtcacagc gccctgcagc cttgatctcc 102720tgggttcaag ccatcctccc acctcagcct cccaagtagc tgggactata ggcatgcgcc 102780accacaccca aataattttt atatttttaa tagagacttg gccaggccag tctcgaactc 102840ctggactcaa gtggttcacc tgctttggcc tcccaaaatg ttgggattac aggcgttagt 102900caccgcatcc agcctcagtg tgatgttttg atatacatgt gtgttgtata catatacagt 102960taaggtaatt aatgtatctg cttttatttt ctaagtactt gcaagctcct cgcctcataa 103020ttccttctag aactttcctt gattgaaaat ctagtcccct gaatgaaggt ggacatacca 103080agacaggatg agggaggtgg aaaggatctg ttccttgtag tggacacacc tggtttgaat 103140ctggctattc tttttttttt tgagacggag ttttgctctt gttgcccagg ctggagtgca 103200atggcgtgat cttggctcac tgcaacctct gcctcctggg ttcaagcgat tctcctgcct 103260cagcctcacg agtagctggg attacaggtg cctgccacca tgcccggcca attttttgta 103320tttttagtag agatgggttt caccatgtgg gccaggctgg tcttgaactc ctgacctcag 103380gtgatccacc cacctcggcc tcccaaagcg ctgggattac aggcgtgagc caccacgcct 103440ggccgaatct ggttcttctt tagctcaggc agagtaatcg tattaccttt tttttgttgt 103500tgtcataaaa taagataatg cttctctaag aaataagtag gctgtgcaca gtggctcacg 103560cctgtaatcc cagcactttg ggaggccaag gagggcagat cacgaggtca agatatcaag 103620accatcctgg ccaacatgat gaaaccccgt ctctactaaa aatacaaaaa ttagctgggc 103680gtggtggcac gtgcctgtaa tcccagctac tcagaaggct gaggcaggag aattcctgga 103740accagggcat cggaggttgc agtgagccga gatggcgcca ttgcactcca gcctggtgac 103800agagcgagac tccaccttaa aaaaaaaaaa gaaagaaaga attatagtgg agactattaa 103860agagcttgag tgtatccatg ttgttgggtc tacatgtaaa aacaatatat atatatatat 103920gtatatatat atatatgtgt atatatatat atgtatatat atatgtgtat atatatatat 103980gtatatatat atgtgtatat atatatatgt atatatatat atatatatat aaataaggaa 104040cttaactata cataattttc tgcagtatga aggctgaaca gatcttaatt ttttctacac 104100atttttgttt ttactttagc atgcatttga ctgagtcgta aagtttttaa gggtgtgaga 104160tatgcttttt tatgttttaa tccccaattc agtgcctgaa tgctgacggg tgctacacag 104220acagtgaatc agtgggtctg tgcaaacgag ggccagtgag aaacacccta gtgtcctaac 104280tgaaggaaga ggccatttcc agtgacagca cactcatcag ttcacctgga gtaccggagg 104340cacgttttca ggttgactag ggaaccaatg aggcttctga caagcagggg acttgggtgc 104400agggccagca ggagcccagg cctcctttct agcaagctgt gctgttctgc tgggcttctc 104460ctgctatggg ttattgagtt ctgtttcctg agaggcataa ggggaaagtg tagacccaac 104520tcctcagatt gtcttaaata atttgagtaa ctaataggca ttaagtaatt tgagcaaata 104580ttgttctcca gaggttgaga atcctttatt gtccctttcc tcccaggacc actcagtata 104640atgttggaag agagccttct gtccttatcc aggtgcaggg aatgaaccag atgattcaat 104700tcctacatac cttttttttt ttttttttta aacagagtct cactctgtca cccaggctgg 104760aatgcagtgg catgatctca gctcactgaa acctccacct cccaggttca agagattctc 104820ctgcctcagc ctcccaagta gctggggtta caggtgcctg ccgccacacc tagctaattt 104880ttgtattttt aatagagacg aggtttcgcc atgttggcca gactagtctt gaatgcctaa 104940cctcgagcaa tccgcccgcc ttggcctccc gaagtgctgg aattacaggc gtgagccacc 105000acacccagcc acgccctttc ttagactaga ccctgaaaaa ggatcccttt tcatcactcc 105060cactgctctt aaggattctc cctgctgtgg tctaaatgtt cgtgtcccca gaattcaaat 105120gttgaaatcc taacgccaaa ggtgatggtt ttaggagggg gtgccttagg gaggtgatga 105180gctcatgagg gtggagccct catgaatggg attagtgccc ttagaaaaga agccccagag 105240agcttcctca tcctttccac caggagagga tgcagagaga aggtgtcatt ctttccactg 105300aggacagtcg agtgcaaaga gccagaacga tctgggttaa catcccaatt ctatcactta 105360tttcactgtg tgtccttcag caagttactg aattgattgt attttctgtt tatttaacaa 105420atatttatac aaagagctta ccattgccaa gcattattct aagtccttca cagatgataa 105480ttcatttaat gcttgcaata actgcaaaaa atagaaactc tcagtatcca agttttatgg 105540cgggagcagt ggggagactg tgaagtcact tgtgcaacgt cccacagcta ggaaaaaagt 105600aagcaggact gcaacctacg tatttagtac tagaggtgtt tagtacccga gtgagttcac 105660ttaatccgct ctgctatgct actctagatt tccgtaattt gtaagtaatt tgtaaaataa 105720cagcttggca aagattgctg tgagaattca gccaatgcta gctagcttcc ttctcccctt 105780ccatttcata gagagggtaa agtaacacac tggtttgagt attagtgaaa gatgatggca 105840tttagaattg taaattgagg gaacaaagta atcaatgata tcatgagaag caaaaaagac 105900ttttttggac ttttgcagtt gtactaagtt tctatggttt taccatttgt tttctggaaa 105960gattaaaaac cattttgttg aactggtaat tccaagcttt taattttgtt ttcaccaagt 106020gtttgttgct catttattac acacattgaa tttgccccag tttttcccta ccacaagtat 106080ctaatacagt gagataggtg ttttgcttct gcccatggtt ttttgggtaa tacctgacat 106140gctaacatta ttctgttatt ttttcagttc attattaaag ttgtttgggg gaatttgaac 106200acatctgatt cctctcagga acgagagggg aaattgtcta gcagctggca ccctgagaac 106260tggtacaaat tactatgtcc gatagtttat cttgaactac ggtgtttata actaccaaca 106320gagcatcaat ttaacaaaaa ttgcagtact tagtattaaa agaggcttgc actttaagga 106380agaatgtgga gaattttctt ttccattaca cacgtactgg ccatattttg gcaacagagt 106440ttccagtgtc attggctctg aattgaaaaa catgagtgag tatcctttcc tatgtacagg 106500agatttccga ggtacgtaat gtgattgtta ttcattattc aggttacttc tagagctctc 106560taagcaagat gtgcatgggg aaataaatat gaacgagaga gaccgtctgt gtccttctga 106620tgcttcattc tgaagacgga aggccagatg taacaagcac agtgaataca gtgctctggg 106680atgtcaaaaa gagaagggat aatttatgac cggggcacca ggaagccctg tagaagtggt 106740gggattgggc caggtgtgat ggctcacgcc tgtaatccca gcactttggg agaccaaggc 106800aggcagatca catgacgtca gaagtttgag accagcctgg tcaacatggt gaaaccctgt 106860ctctaccaaa aatacaaaaa ttagctgggc gtggtggcac atgcctgtaa ttccagctac 106920tcaggaggct gaggcaggag aatcgcttga acccaggagg aggaggtttc agtgagccaa 106980gatcacacca ttacactcca gcctgggtga ccagagtgaa gctccatctc ataaaaagaa 107040gtggtgggat tgtagctgag ccttggagaa taaataagac ttggagaagt tgtgatgcct 107100gaggaagact ttccatgatg aaataaagaa acagccacag ccagagctca gatgagagga 107160aatttaagga ccaggggtcc ttttggatga agcatgaagc acagaagaat ggagggaagg 107220aaagggatgg cagtctgcat gagaaaggac ctgaaatggt attttctgtg tgattgacta 107280ttttctttca ttgagtgaag gttttgaacc aggaaatgaa atcatcagag cagaatttta 107340acgcaaacta atctggcttg aaactaaatt aggctggagt aaatgttcaa gagtgtgaaa 107400gtgaggtcat ctgttgcaat acttgggcag taagtagcag gggcctgaca tgttaaggtg 107460accataagac tagaaacaag atctctgcaa ggaaaagaat caatgaggct ggacaaataa 107520ctggagacaa gagaggagat gggtgataaa gggagtgctt tctaatgtgt attggtagat 107580gtttccagtg acactactta gtatcacaga tgttactgag actttgctca agtcaacttg 107640taattgcatg cactgatttt ctctgtaaga cttctcagag cctataatat gctaacatgc 107700gctgtgacac tccaagaaga agatggtgtt gtctaatttc ccatacttat ttgtccacag 107760aaactctttt ttaaaaataa gctaggcaca gtggctcatg cctgtaatcc tagcagtttg 107820gaaggccgag gcaggaggat cgctggagcc cagaagtttg agaccatttt gggcaataca 107880gtgagaccct gtctctaaaa aaataaaaaa ttaggtgtga tggtgtgcac ctgtggtcct 107940agctacttgg gaagctgagg taggaggatt ggatgagcac agaaggttga ggctgcagtg 108000aggctgtgat tatgctgttg tactccaccc tgggtgacag agcaaggctc tgtctcaaaa 108060aaaaaaaata aataaataaa aagttgatag gtaaatgtgg aaaggatcta ggattttctg 108120gaacactatt ttgagacatg gtgtactaca tttggaggat gatggtgtga tttataaaaa 108180ctgggaagtt gacattagaa tatggtttcc actaaagata agttgacatc accttatctt 108240gttgttaaaa tgtgttaaat ttgaggcacc atagaccgtg tatttggaag catggagaaa 108300gttgcatgtt ttgtggaagt agagctaaga tgagagattt ggatagcaag attaatctgg 108360ggttatcacc taggggtgat cattgaattc ataaaggtga aatagtaggg aaaggagaat 108420ttgagtggag cgaatgacag aatgttggga aagatctgta agagacagaa gaactggcaa 108480aggctaagaa acagtggtca gggagaagga gaattcggat cttgcatttt cagcaaacta 108540ggataggact ttgcagagtc agggagtgtt cagcaacgtc aaatgctgca gagagaggag 108600tgggggagct ttaaagtttg gaaaataaga catgatcact ggctttggaa agaattgttt 108660tattagaaca tggtaggtgt ggaagcccaa tttaagaaat cttggagtga ctttgaaaaa 108720aaaaaaggca tgaacagaaa gtacatcaca tccctggtta gagcattgtt aatgatataa 108780aataaagatt taatgaaagt ttagaggaag aacaagattc agatttcttt ttaaaaaaat 108840ctttacattt tgctcaacac agggaaattc tttgcctcca aacccgagag ctatttttgt 108900ttttttttaa gggcatgggc aaaatgaaca ggattaagta gaagaaaaga agcagaaaag 108960aagggataat ggagagggaa agacactttc tttttgtttt ttaatagaga tagggtcttg 109020ctctgttgcc caggctggag cgtagtggta cagtcacagc tcactgtagc ctcaaactct 109080tgggcttaag cagtcctccc acttcagcct cccaaagtac tgggattata ggggtgagcc 109140actgtacctg gacctactac taaaatccct ggaatggtag ttcttaaatg ttagcttggg 109200ccgggcatgg tggctcacac ctgtaatccc agcactttgg gaggccaagg tgggcagatc 109260acctgagacc agcctggcca acatggtgaa acaccatctc tactaaaaac acaaaaatta 109320gcagggcatg atggtgggtg cctataatat tatccagcta ctcaggagca ctgaggcagg 109380agaatcacct gaacctggga ggcggaggtt gtggtgagct gagattgtgt cattgcactc 109440tagcctaggc gacaagagca aaactctctc tcattttaaa aaaaaaaaaa aaaaaaaaaa 109500gaaaagaaaa gaaagaaagg aaagaaaaaa aagttagact acagaattcc ctgcaggact 109560tgtttaaata caggcttcct ggcctcagcc ccagagtctc tgttcatcag aaactgggta 109620cagcctaaga accggccttt ctaacaagtt cccaggtgat gctgatgcag ttgacctggg 109680gatgacaatt tgacaacctc caccccagag aagggaaaaa tgtagaatct aggttgcagg 109740tcaaggtgtg ccctgggaga gagaaaaggc acatctttgt caggaagtta ccaggaatga 109800aagaagaaag ggtgaataaa aatacataac aatttgaagt gcagttagaa gagagatgac 109860agtcactgga attggagaat tatgtcctag agtctagaaa gttgtcactg tagattgtga 109920agttgtgaca gtgaagatgg acaggtagag atgatactgt gagcccagtg ctgaaaccat 109980ctaggaaggt gtctgtaagt gaacaaatgg accttatgaa gataggtcat gtcctatggt 110040ctggtaatgg tgttggggca gcagagcccc taccaaattc cttatgctaa aacgagaacg 110100tgcccaggtg cggtggctca cgcctgtaat cccagcactt tggggggctg aggcgggtgg 110160atcacgaggt gaggagatca agaccgtcct ggctaacacg gtgaaacccc gtctctacta 110220aaaatacaaa aaaattagcc gggcgtggtg gcgggcgcct gtagtcccag ctactcggga 110280ggctgaggca ggagaatggt gtgaacctgg gaggcagagc ttgcagtgag ccgagatcgc 110340gccactgcac tccagcctgg gcgacagagc gagactccat aaaataaaaa ataaaaaata 110400aaacacacaa caacaaaaaa cagagaactc cagttctacc tctcctgcaa caagtcggga 110460ggaacttgct gtgatgaagc tgaatttctc tttaagccaa gacagagtca gagggggttt 110520gctaagaacc gtagatactt acatcgtgct ctgcaaaaac tccctttttc tgtcctcaca 110580aggaccctat gggattcctg ctattatctc cattttacag ttaaagaaat aaactaactt 110640gctaagttca caactactga atgaggaagc caggaacgga gtccgagcag tctatgggaa 110700cttgacagaa tctctctctg actgttggca cgtgggactt cccacttccc ttaaccatgt 110760taaaggaacc cggagcatat cccaggggct gcactgcatg ccatagggtt gttcccagag 110820tggcactgta tgattgagga tgcaggccag ttacagcaga gaaggggcct cagaaaggtc 110880acacaccagg accagtgttg agaacaagag gtaggatgat gcaggctggc tcagggccca 110940tgaagggctg gagcccattc tcaccagcgt ggaagacccg gggctggcgg catgaggcag 111000aaagaaagca cttcatcagg ctcaatggca acaaatctct tcagtggaaa agttctgctg 111060tttcctctgt atatcccttg ggtctgtatc attctcaaaa tgagaagtct gtaaagccca 111120aaactatccg gttaatgtgc agttccaacc agcttaaaga ataaacggta ggtaaaatgc 111180ctgtgtgttg tatttctctt tatgtctgcc ttttaaaaac tgaaagtgca tgtttactaa 111240atcacagggt ttgagttgag gaaaacacta ccgttgtatg cctacaggca tgcattaagt 111300agggctaaaa gcatccttta gcaaaagtag tttcctcttt tccttgctaa atatcttgta 111360tgcattgtac ctgatcacct aaaagccagt gcttttagta attattgaag aataagaagg 111420gcctgtggtg aataatgtcg aaacagtgtg atgggggagc tcacacgtgg ttgcagttga 111480aggcagagaa gatgtgggcc gtaaggaatc ccccaggact gggtgttcct tctcactgct 111540tcttcagaaa agcattgtca gagtacggga gagggagcaa aatagtgtaa aaatgaccct 111600cgccagctcc agctgctttt ttttcttttt tttttttttt ttgaggagtc taactctgtc 111660actcaggctg gagtatggtg gtgccatctc ggctcactgc agtcgccgcc ccctgggttc 111720aagcaattct cccctctcag tctcctgagt agctgggatt acaggtgtgc accaccatga 111780ctggctaatt tttgttgctg ttgttgtcat atttttagta gagacgggtt tcaccatgtt 111840ggccaggctg gtctctaact cctgacctca ggtgatccac ccgccttggc ctcccaaagt 111900gttgggatta caggcatgag ccaccacgcc cagccccgtt tgcttttttt atgaaaagaa 111960tcactaccaa tattttaaca tgttacttgg gacatatttc agcagctagg ataacacata 112020ataggggaat cactgggaaa ttttatcatc ttacctatac atgcgataaa aatctattaa 112080agtcagctct atatcttgga gcagaccttg ggggaaaatt agtgcagagc tgggatttga 112140aggatggtgg gaattggaca catttgagca tttcctcagt ggttcttgca tttctcctta 112200tttatcagcc tcctgatgtg agaaaagtaa ctacttaatg aagagatcca aaataaagaa 112260gcagccatct gggtagttta atcttataaa tacccagctg caattttaag aagagattta 112320gaagcatagt ggtgtgtctc gggttcctgg accaggtaga cccacaagct gtcacctccc 112380acctggcccc tgctctgttt cagattttgt tcattgcagt catggataga gagtttctgt 112440catgaagaca catcacatct gccacatcag tttagcactg ttctaaacta ggttgcgaca 112500gaatttgttc aatgtgaagt ttttctgatt tggtgtatat taggaaactc aatgtcttta 112560gggctgtgtt tttccgataa acacattttt cccctgcaga ggatgtttga ccattttctt 112620tgttactcct ctcccagaag agaggaaact tctcacctgc aaggactttt tgctctgcag 112680ttcccttcac actcacatat tctcctgtgt cacagctctg gggtatgtct tatgctcacc 112740catcacgtag ttcgttatca ccactacaga tattgacaag ctagtgaaac ctgcgttttc 112800attgcattta actttgaagg cacacatctt tgaacagaat gctaggtgct aatgtcatag 112860cttacatcta gatagggctc tatttatgat gctgttatgt tgtcttcata atctattgtg 112920gtattcagtt tagctcataa cagctgtata aattcaggtt gccaagggta aaagaggtga 112980cccaacctcc catctggctg tattcccctt gcacactcat atacctgtct cttctcaatg 113040ccctaattct atctcctata aaaagtttgc atcttcatga attattccta tcttagtaaa 113100ttaataaatg gcctttcaga acaagtcata ataaaaatac agctgggcat agcgatgcac 113160acctttaatc ccaacacttt gggagactga gcaggaggat tgcttgatga cagatgctcg 113220agaccagctt gggcaacaaa gtgagaccct gtctttacca aatagaaaaa aaattgaatg 113280ggcccacgtg gtggggcatg actatagtcc tagctactta ggaggctgaa gcaggaggat 113340tgcataagcc caggaggctg aggctgcagt aagtcatgat ggtaccactg cactccagtc 113400tgagtggcag agaaacactg tgtctcaaat aataataagt agaaccatag cacatttaaa 113460aaaaaattat ctagctaata tcacatcaaa agactatctt caattaatct ttgcaaaaat 113520aaaggttatc aaggttgacc ttttttaatt taatatttta aagtcagggt cttgctctgt 113580tagccaggct ggagtgcagt agcacaatca tagctcccct gtcacctcaa actcctgtgc 113640tcaagccatc ctcccacctt ggcctcctga ggagccagga ctacaggtgc acaccaacac 113700accgggcaag gttagcctta tagacagaag ttctctacct aggatgattt ctatattttt 113760gttcaccaaa aaaggttctc tggcctacca gcagcaccct ggctgctttt gctgactcta 113820gtcattcctt aatgagtata cctggtagat tcttactgga ctacacctta gtgcagaagg 113880atattagaat atcccacgct tgatagcaga cctggttgaa gaggccaaca caaaccagag 113940tcagggataa aaccaagata aaaatctcat ctccgctgac atcatagcat ccatgcagtt 114000gctcaagccg aaagcatcct cgattcctct ctgtttctct cccctgctca gatgatctgt 114060actgatccac ttttctccat ctctgctgct acctcattag tccaagccat gttttgtctc 114120cctggaacta ttaatattag aacagccttt tgacctgtct cctcctctct ctcttgcttt 114180tcctgatttt ctctccccac gacagctgga gggattttct taacacttaa attggatcct 114240gtctctgtcc tacctaaaaa cctattgtga ctgtctattg ctcttagaca aatcaaactc 114300cttctcatgg tttaaaaggc agtacgtaaa ctagctcgtg tcagcctgac tttccagtgt 114360ccttcctgcc agtcccacct cattgcctag caggtactct tgtctccctc catcgcctcc 114420acaggcctgc tcccttgagc tctttctgtc cctccatgag gcttggaatc tctcccctcc 114480tacctactca ctgtcatcta tgtcccaact cattgtctcc tcctcagaga cttcttgagc 114540actgccactc gtatcaccct gtttatttcc tccacagaac attaatgaaa gagttcatag 114600attgtctcgt acacatactt accaatttct tgccttattt tattttgaga cggagtctcg 114660ctctgttgcc caggctggag tgcagtggcg cgatctcggc tggctgcaac ctctgcctcc 114720caggttcaaa tgattcttgt gcctcagcct cccaagtagc tggggtcaca ggcgtgcacc 114780accatgcctg gctaattttt tgtattttta gtagagacca ggtttcaccc tgttggccag 114840actcctgttt gtcaaacttc tgacctcagg tcatttgcct gcctgagcct cacaaggtgc 114900tgggattaca ggtgtgagcc accatgacca gctgattact tgtctgtttt taaacaagta 114960gcatggaggt gcctggaggc ctgcgtttgt gtttcacttg tttctgattg tactcccagg 115020aacaagccca gcgcctttcc cacaataggt attgtataaa taatgaatga atgaagacag 115080tctctattct cttgaaatgt aaacaggaca gtgaggggta aaataaaatg gacagttatc 115140atgtagagtg atgggtgctt tgatgggaga agggttctgt cccttaggaa cattcagggg 115200aagtagtacc aaatactcag gaattagaga aagggtccaa cagaaaatga tatctacaga 115260tttcaaggat ccctaataag tacctatatt gtgttactgt attaacatta atgtttcatt 115320taaaaccagc gtttttcaac ctttgtcatt aatcgccccc ttaaggggcc ttttctaggc 115380agtttttttc ctaatcacca tccctccctc cataaaattt cataccacag acatgtctat 115440accaccgtgt atttttctag gtactgtatg tgtatctcta ctttatactc aaaatgagta 115500agattgtttt tttaccccct aagcaacaaa cgtttgcccc cttggggatg agattgcccc 115560tgttgggaat gcatatatta aagcatttaa tatcaatcat aggaccaagt tttaaagaaa 115620atgcagttgg tacgtctgca cagtacttgc actttaaatg tcaatggact gtgtagtatt 115680tcaaaagcac acacatgcac aattttcttt ccaatggagg agacagatga tactggtttt 115740ttcaaaagtg tgatatcgat ggatactgtg gctcacgcct gcaatctcag tattttggga 115800ggccaaggca ggagaattgc ttgaggccag gagttcaagg cctgcctggg caacatagaa 115860agactgcccc ccgctacaaa aaaattaaaa aattagccag acgtgatagc aggcacctgt 115920ggtcccagct gctgtggagg ccaaggtggg aggatcactg gagtgcagga gttcaaggct 115980gcagtgagct atgactgtgc cactgcagcc ttgggtgaca gagtgagacc caatctctta 116040aaaaaaaaaa aaaaaagtgg tattcatact cattgatgtg aaaagtcaac accaaagtca 116100taatgagagt tcacataata atatccacca atatgtgcct ctggaaaaag cattaaaagg 116160tgcatcagag gaacttatat ttttaggaaa aagtgtgaca gacaaatgca cagcagtcat 116220tgcacagttt ggaaaggaac atgtgggggc tggttgactc caagagtgct tggtgatagg 116280ttgggttggt gacttagtaa gaaagtgttg cctaagactt tgatttagaa acggcactta 116340agttattagg tagatgagtg ctgatagagt tacttggaag aactgaggta ggagagctgt 116400gtttgaagat tcctggagat aagaaaacat tttcagagaa agagtgagta atctggttta 116460cattctgcta ttgctgttgt tggaggttaa ttctgttggg aacacctgtg ttcacacagg 116520taaataccaa ctggctcttc cttaggggat aaatagtgta taaagttggt gtagaaaatg 116580ctggtgaagg agtttttagt aatggaactt agcccaaggg caaattgtat atcactcaac 116640acttttcact tgcttatctt tcaggaattt taagaaggtt aaggcactaa ggttccttaa 116700gtgttttaac tctgcaggcc ccatctgtgt tccagctgct gttctgcaac tgcaaatgga 116760ttctgcttta gtatgataat aagaaggtca caacccagca aaagtggtgg gcttctaact 116820ctatcctttt agcagaattt cttttcatta gctgagtaga attgagcttc actggttggc 116880agtgaaatga ggcatggcct agcttgatgg tccatgaagg atgctcactg agggtgacat 116940tttgttccat taaagctgat gtttctattt ataccaagga tagtttgtgc agttacaccg 117000gaaataagat atttcctgcg tttacagaca tctacatgct tgcctttttt tccatttccc 117060actgaaccag tctcgttgtt gtcaattatt cttcttcctc tatttaatgg aatctttact 117120ttcttgacag atgaatgtac atgaccactt tcataatgcc tctcccttaa gaaactgcct 117180actgatcttg gccaggcacg gtggctcaca cctgtgatcc cagcactttg ggaggctgag 117240gcgggcttat catttcaggc caggagttcg agaccggcct gaccaaaacc ccgcctctac 117300tgaaaatagg aaaattagcc aggtgtggtg gtacacacct gtaatcccag ctacttggga 117360ggctgaggca ggaggatcac ttgaacctgg gaggcgaagg gtgcagtgag ccaagatcac 117420acccactaca ctccagcctg gatgacagag caagactcca tctcaagaaa ataaaataaa 117480caaccagttc tcatgagaat cctatcacga gaacagcgcc aagatatagt gctaaaccat 117540tcataggcca cccttatgag ccaatcacct cccaccaggc cccacctcca acactgagga 117600ttacaatttg acatgagatt tgggtggggt cacagatcca aactgtatca ctctccattc 117660ctccagccct agcaaccact attctacttt ctgcatctat gatgttgacc attctaacta 117720cctggtatga gtggaatcat atatttgtcc ttttgtgact ggctaatttt acttaggata 117780atgtcctcag agttcaccca tgttatagca tttatcagaa tttctttcct ttttaaggca 117840gaataatatt ccaccgtatg catataccac aattgggttt atttgattca tctattggtg 117900gacattcggg ttgcttccac cttttggcta ctgtaaataa tgcagccttc ctcctgttcc 117960ctttctcccc ttaagggccc ttcagacctc tgccttctac cactgggcat agtctacaca 118020cagatcatga cttttggcta ctgtcagtaa tgatgctatg agcatgggtg cacaaataca 118080cctccaagaa ctttgggtgt tgggtgtatg tttagacaga gtttcactct gtgtccaagc 118140tgcagtgcag tgtgcagtca tggttcactg caggctcaac ctcctggggc tgcgtgatcc 118200tcctgcctca gcttcctggg taaccaggac tacagacatg cgccaccaca accaactaat 118260ttttcatttt ctatagagac gggacctctg tatgttgcct aggctgattt tgaactactg 118320ggctcacagt atcctcctgc cttagcctcc caaagtgctg gaattacagg catgagccac 118380cacacttggc cctccaagaa cttttgaaat aaactctatt aattatattc agaaaactta 118440atgctatcac cttagactgt atagcataaa ttaagttcct cccacatagc aatgcctcct 118500tttaagatgc tgctgggtaa tagtccttag tttaaatatg tcccagctct ccttagcaca 118560gcagcagtct tttacattaa ctttttagag taaaagaaaa attaggagag ggccggtcgc 118620ggtggctcat gcctgtaatc caagcacttt gggaggctga ggtgggcgga tcacctgagg 118680tcaggagttc aagaccagcc tgaccaacat ggagaaaccc cgtctctact gaaaatacaa 118740aattagtcgg gcatggtggc acatgcctga aatcccagct acgtgggagg ctgaggcagg 118800agaatcactt gaacccggga ggcggaggtt gtggtgagcc aagattgcac cattgtactc 118860cagcctgggt gacaagagca aaactccatc tcaaaaaagg aaaaaaaaaa ggagaaaatg 118920tttggagaag tactaaatat aaaaatcaac ttatttttag tagaatgtat ttgttctcaa 118980cacagctaat tgttttagat aattaccagc cttggactac agacacattt cactaagaca 119040gaaaattatt tttgaaatat tttgatgatg cagagaaatc atttaaccaa ttctgctatc 119100tcattgtctt ttatgaaaca ctccctttaa aaatatacaa gtattttaga ctatgcaaac 119160atttacgctt gtttgaaact aagtaataaa atctattaag gttttctgtt gcaaatgaat 119220tcatatcttc ctgtggagtc ataatttaag ataaaaatag tgagattcaa gttcaaaaac 119280aaatttggca aatcaagtat tgtaaaagtc taggaaatgt gaaaatttta ttctagttga 119340ctttcatgtt ttactattaa tttgcatgtg gtgaattcct acttgcattg attctaaaag 119400gttgtataaa ctgtatgagg atgggaattg tgtctgtttc atttactgta tgctcagtac 119460atactagtac tcaacacata ctagtctcaa tacatgctgg gtacatacaa atgaataatt 119520acaacagtat catttagaaa ctcttccata gtctttcttc atgccttaac atggaattaa 119580taaacaaaat tttaagaaat gtcctacata taagacaaaa aggtcccaaa ggatataaat 119640aatagtccta ataaaatcaa gatttttttt cacttgttat tagcatccta actgcaatgg 119700ccaactaatt caaaggccta tgcatactat tatactatta acagtaggaa aaaagtaagg 119760tgaacttttt tttttttttt tttttgagac agagtctcac tctgttgcct aggctggagt 119820gcagtggtat gatcttggct cactgcaacc tccgcctccc aggttcaagc aattctcctg 119880cctcagcctc cccaggtagc taggattaca agcacccgcc atcatgccca gctaattttt 119940gtatttttgt agagatgggt tttcaccatg ttggccaggc tggtcttgaa ctcctcacct 120000caggtcatct ccccgcctcg gcctcccaaa gtgctgggat tacaggcgcg agccactgca 120060cctggcccaa gatgaacttt cacaaacatt ttttaaaaag acttctgctc ctgttgatag 120120ctcaacattg aagatactat gaatttttag ttaaaaattt taaaaacata cagttttggc 120180ttctgtcaaa agaatattgc tgcctcattg tcacagctat agccaagaac attaaaatct 120240catgtcctaa tgacactaga aacattagac caattgtcat ttgcccctcc cagctatcat 120300ttttctgttt cttctgcaga aatatcacta tcctttggaa caattttgaa ggaaggaggt 120360ggctgagatt atcccatggg acttgtggtt ttctggactg agaccattct ttcagagttt 120420catggggctg accgccacca caccacaaat cacctcaatg ctgggaatac ctgaatcatc 120480caggctttac acagccatac agtggtgctg ccagttagga gctaagaatt tctcacctgg 120540ctgtggtgct ctatggcatg accaatgctt gggtgaaatt caccaaaaac agcaaatagt 120600tatttctccc ttttttatta ttatgtatca tattattgct aactaaaaag atccataatg 120660ttctagatat gttatgtagt tatttgggca gttcttactc tccagctgtt tgtaaaagta 120720gaatgctggc cgggcgcagt ggctcacgcc tgtaatccca gcactttgga aggccgtgat 120780gggtggatca cctgaggtca ggagttcaag accaacctgg ccaacattct gtctctacag 120840aaatacaaaa attacctggg aatgacgatg ggtgcctgta atctcagcta ctcgggaggc 120900tgaggtgaaa gaattacttg aacccaggag gccgaggttg cagtgagccg agatcgcacc 120960actgcactcc agcctgggca acagagcgag actccatctc aaaagaaaag gaaaaaaagt 121020cgaatactaa catatttggg tttcatttaa gaaaatcata tcttacatta ttatttatgt 121080atgtatatgt ccatacatat atacacacgt acacacatat tctcaaatgg caagtgggat 121140catggacatc tataatcatg cataaaagaa aggcctggga gagattagga gtgttgtttt 121200aattcatgtt ttacctcttt gtttgtggct gtcagtagat taacataaag catgttggaa 121260agaaccaggg attatcttct agcccaatgt attcaacttc tgtttgcaca tttacattct 121320ttctctgaat gatgagctct cggtggttta gtagggataa gttccaaggg aagcctttct 121380caaattcatc acttacaaca tttatacaca cagctctgat agttaagtag gactgacttt 121440ttctaatatg tgtagggata tttcaaaatt atttctattt tttcctgtgg gagttctgat 121500gtatacaagc ctagagattg agtttagtga tggtgccagg gaagattttc ttttcggcag 121560gttaaaaagt ctttgaaacc ctcccattta aaaaaataaa taaaggattc tggttgggca 121620tgctggctca cacctgtaat cccaacacct tgggaggtca aggcgggaag atcacttgag 121680cccaggagtt cgagagcagc ctggacaatg tagtgaaacc ccatctctac aaaaaataca 121740aaaattatta tgtatggtag ggcacacctg tggccccagc tactcaggaa gatgaggtgg 121800gaagaccact tgagcgtggg aggttgaagc tgcagtgagc tgtgatagtg ccactgcact 121860ccagcctggg tgaaaaagca agatctttcc aaaaaaaaaa aaaatccact ttttgctcta 121920tttcttttct ttcctttttc ttttttgaga ccgagtttcg ttcttgttgc ccaagctgga 121980gggcaatggc gtgatcttgg ctcactgcag cctccgcctc ctgggttcaa gctattctcc 122040tgcctcagcc tcccgagtag ctgggattac agtcatgcgc caccacgccc agaaaatttt 122100gtgtttttag tagagatggg gtttctccat gttgatcacc catccttacc tgatctgcac 122160acctcggcct cccaaagtgc tgggaataca ggcatgagcc actgcgccct gcctttcttg 122220ctctatcttt acaccagcat cccttcttca attacacaca attctttcga tccctatcct 122280caaggtgcca ggcgttattc cacctttatc ttttgaactt tatccagagt gactgtcttg 122340ctgtaacaga tcactctctg gtcagacccc tttcaaggct ttaactttct tgtttgatag 122400cattctccct tttcagttcc tttcaaatcc agtgttcatg acttccctga ttctatcaag 122460atatatttat attttttgaa tgcgtgtgct ttaagccagt ggtccccaac ctttttggca 122520ctagggaacg agtttcctgg aagactattt ttccacaggt ggggaggggg tgggagatgg 122580ttttgggatg aaactgttcc accccagatc atcaggcatt agttagagtc tcaacctaga 122640tccctcccat gtgcagttca caacagggtt cacgctgcaa tgaaaatcta atgccaccca 122700ctcacccact gctcaccgcc cccagctctg caggagaccc ctgctttagg catttcgata 122760aagctttttt tttttttttt tttttttggc gttgtaccat cacccaactc tgcaactcct 122820cgaacagtat gagtgggaaa tgccagtggc ttccaatcta tcctttggca ctcgaggagg 122880gagtttatcc agtatctgat aagacatagc tgccttggca tctgcaaact ttcctggagc 122940tctggagaga tgtctgaaat tttatttttt ccatgttggt tgttcactta ttttaggaag 123000agatacctct ccaccctcac agccaggaat ctatcccgtt ctagagaatc ggtactgtgt 123060tttgctcatt ttccccatgc acacgaactt cttgcatgag attcccagca gtcttgattt 123120cataatcttt ctccagagct ttgtaaaatg tccaaggatc ctgggacaga atattcacta 123180taagaaatta ttaagctgtc taaaaacaga aatagctttt acactgcctg taagacagca 123240aataggagcg ctggttggtt gattgattga ttgagacagc gtctcactct gtcacccagg 123300ctggagtgca gtggtacaat ccgcagctca ctgcagcctt gaactcctgg gctcaagcga 123360tcctcccacc tagtcttcca agtagctagc actacaggtg cacatcacca tgcccaggta 123420atttttgtat tttttgtaga gatggggtct tgctatgttg cccaggctgg cctcataatt 123480cctgggctca agctatcctt ccacctggac ctcccaaagt gttgggatta tgggcatgaa 123540ccactgcacc ttgcctagga actatttatt aagcaaatgt aaacagtgtt cactttcata 123600tcatctgaga ggacttttat acctttccct gtccctcaaa ctttgtattc tcttttgctt 123660tcagtatttt tagcatctct ttttactgtt aaaaagtgaa aagtatctct ttcatttttt 123720agtattttat attttttact tacgtatttt attttttatt tttatttatt tatttttgag 123780acagaatctc actctgttgc ccaggctgga gtgcagtgac acgatctagt ctcactgcaa 123840cctccacctc ccaggttcaa gagattctcc tgcctcagcc tcatgagtag ctgggattac 123900aggtgtgtgc caccacgccc agctaatttt tgtattttta gtagagacgg ggtttcacca 123960tgctggccag gctggtctcg aactcctgac ctcatggtcc acccaacttg gccttccaaa 124020gtgttaggat tacaggcaag agccaccgca cccggccact tatttcattt attgacctca 124080cttcatgctc cactaagaaa taaaaacttc acagatagca acagcctcat cttgccacca 124140ccaaatctgt aaacctgcct ttatctgtgc cccttgtgtg ttctccttct tccctcctgt 124200tagaagaagc atctctcctc ctgtcagaat cctcctgttc ttggccaggc atggtggctc 124260acacctgtaa tcccagcatt ttcagaggct gaggcaggag gatcatttga gcccaggagt 124320tcaagatcag cctggacaat atagcaagac ctcttatcta ttaaaaaaaa aaaagttttt 124380gtgttctttg tttctcacct tccaagactt tttctttctc tttttggaat caaggtcctg 124440ctttgtcacc caggctggat tgcaatggca caagcatagc tcactgcggg cttcacagct 124500tcaacctccc aggttcaagt gattgtccta catcagcctc cggagtagct gggattgcag 124560gtgcacgcca caatgccctg ctagttgatt ttgttttttg ttttttgttt tttttttgta 124620gagatggggt tttgccatgg tgcccagact gtctggaact cctggtctca agtagtctgc 124680ccaccctggc ctcccaaagc aggattagag gtgggagcca ccgtgcctgg ccccaagagt 124740tcctatcctg cagcatctag tcatcccctc tctactggat cactccatca gcatgacata 124800ttatggacat ttggagtggc ccatgatcag agcccaaaac accacatccc agctcctccc 124860gctccatgca gcctcctagc tctgcccccc ttcccagcca ggcctttgac aaatgagtgg 124920tctgcactta acttctccct tcctctcctc ttcatgtcca gatattcctc ccacagctct 124980aacacattgc tcccaacaag gccactaaca acctctgtgc tgtcagagaa gtcataaaca 125040tcgtcttatc ttacccaagc tgtcagcggc cttcacaatc gttgaaccct acctccaact 125100tgacatgttc tctgctcttg gcttctgaga caccatgttc tctggacatc tcttgccagt 125160tctttttttt tttcttttgg ggagggagtg gtagggacag acagactcct atccctctgc 125220ataacttctc aaagttaggg ttctttagca cttagaacct ggctcttttc attagccagg 125280tgtggtggtc catgcttgct acttgggagg ctgacatggg aggatcacct gagcctaagg 125340aggtcgaggc tgcagtgagc agtgatcaca ccacagcctt ccagcccggg caacagagtg 125400agaccctgtc tcaaaaatta aaataaaaaa aaaaaaaaaa agaatttggc tcttttctct 125460ttacttctca ctcttagatt caatttttat ttccaagctt ccccacttgt atattcacac 125520ggacctcaaa cttacccaaa tcggagcacc tgattgccca cctctaccac ccaacatgtt 125580cttctctcag gctccccctt ttcaataatg gccctagcca tcaataagcc ctgttgtttc 125640tataataact ctaaaataca cccagcttca ccactaccac acatctccaa gccaccatcc 125700actctcatct ggactctgct tctgctctta ttcctttcca acgtgttcaa aaagttaaac 125760tggagaaaat tgacagcatc tgcatttacg caggggcggt aggaatggga gaggagggga 125820ggcattttaa aactatgtat ttagtggtta gaacatgcag attctagtga accaatgcaa 125880agtgggaaag aaccaagagg aaggagtcca ggttaaaggg atattcaaca gaatggggca 125940gatgggaagg ccaggctttg aacaggtgac ttcgtcttgg gacttgttga gctgtgggag 126000gatcagatgg atattcttgg aggtggttgg atatagaaaa ctagaaacag ctgggcatgg 126060tggctcacgc ctgtaatccc agcactttgg gaggccgagg caggcagatt acctgaggtc 126120aggagttcaa gaccagcctg gccaacatag tgaaacccca tctgtactaa aaatataaaa 126180attagctggg cttggtgatg cacacctgta gtcctagcta ctcagaaggc tgaggcagga 126240gaattgcttg aacctgggag tgggagtttg cagtgagcca agatcatgcc actgtgttcc 126300agcctgggcg acaaagcaag actctgtctc agtaaaaata aataaactag aaacaagact 126360tcagggctaa atatgtacat ataggaatca tccatgatag gatgtacttg aagccatgga 126420ggcaaataaa atcacctggg aaaaaatgta cagatgagaa gatgagagtg taatcctgct 126480tcttgccgca ggttgagttt ttggagcacc ctcgttccac cttctagtcc caagcgtgtg 126540ttcctcttct ctccatagca ttaccctacc ttcatgtggt cctggttact tcctcccagg 126600atgcatgcaa aaggaagcac agtgttccac attccctttt agtcttgttg atcccaaaga 126660taagtacgtc atccaacttc ttcttttttt tttttttttc aagatggcct ctccctttgt 126720caaccatgct agagtgcagt ggcatgattg cagatcactg caacctctgc ctcccaggtt 126780caagtagttc tcctgcctca gcctcccgag tagctgggat tacagcatcc accacctcgc 126840ccagctaatt tttgtatttt tagtagagac agggtttcac catgtttccc aggctgctct 126900cgaactcctg acctcaagtg atctgcctgc ctcagcctcc caaagtgctg ggattacagg 126960cgtgggccac tgcgcccggc cccaacttct ttataaaggt tttgttactg aacattttgg 127020cacttaataa aattatctaa acctcccaag agagtgacaa cacttgacaa acacatacag 127080gcacacctcc ctaccctggg gatgacttat ctgacattcc agttacggtg cacgaagcca 127140agaatcccta agtagcaaat aaaacctttc aaccattgaa gtagagttat aacttccaga 127200cactaaagct ggagttcgtc ttttgcctat ttaatctata tattttttat tttttagaga 127260cagtgtcgct ctgtcaccca gcctagagtg cagtgacatg atcatagctc actgcagcct 127320caaacttctg gactgaagca accctccagc ctcagccttc tgcatagctg ggactacagg 127380tcccagcaca ccaccatgcc cagctaaatt ttttacagag acgaggtctc actatgttgc 127440ccaggctggt ctcaaactcc tgaccacaag caatgctcct gcctcagcct gtcaatattt 127500tacctgtatt tctcatcagc ttggtttttt ggtcatggtt gttaattttt gaattatatg 127560aaagtcctac atagaaggtt ctctcctggt gttttggcag atgtaagaaa attgatttcg 127620tttgtagctg ttaatttgag aatccattgt agatactagc aatttctgaa ggaataagag 127680attcacactt taatgggact tcacaatctt tcatctaaaa attctagctc ctggtgggga 127740gacaagttcc agacccacaa ctattagaaa tatgttaata aactgggccc cggattgaag 127800catgtctaaa caaactatgg aatttaataa tgtccgaata atgtggaaat aatgttttca 127860gtggaatagg atgaggctgg gtgtggttct cacacctgta atcccagcac tttgggaggc 127920cgagacgggc ggttcacttg aggtcaggag ctgagagcag cctggccaac atggtgaaat 127980cctgtctcta ctaaaaatac aaaaattagc cggtcatggt ggcaggtgcc tgtaatccca 128040gctgctaggg aggctgaggc gggagaattg cttaaactca ggaggcggaa gttgcagtga 128100gccaagatca caacactgca ctacagccca ggcaacagag caagactctg tgtttgttaa 128160ataaataaat ggaataggat gaaacagaaa aacattgtca ttttttgaaa actctgaaat 128220aatctattct aaagtaattc aattgaaata ataaattcac tttgcattct ccttttgcca 128280caagattcaa atatttacta actggcatca acactggtgt tcaaattcag tattggcaat 128340ttcacacttg aagactgtct gtgttcccag agagtgttaa gtctaacttt gaggtccaca 128400aatatttatt ggtgtctggt gtatgccagc aactgggaaa acaataggag gtggttccta 128460cctcaaaaag cttgcagttt ggtgaattta cagtctagta agactagacc tacgtaaaag 128520atacagagca atgcaaaaca gtagatgtca gatgccattt gactaacata cgcgttaatt 128580gctgcaggat ttctagggaa ggagaatctg gtgggctgca gtaggagaaa ttggaaaata 128640atttactatt ctccattgac agtggcagtc aggaacctgg gagcccaatt tatttgtccc 128700ctcaacccaa ctcaaatggg tcctcccttg ggaactttgc tgccaacttg ctcctcttct 128760aaacccctcc ccaggcacag tctatcaacc ccaactttgc aaaacttcca ttttttgttc 128820ttaactatgc taaagtactc acaatgcatt gcaattgttt gtaagcctgt ctcctctaca 128880agattctgat ttcctgtttt gttatgttca tctttgaatt cccctactgc ttagtatgat 128940acatacaata ttgttagtgt ccccaaaaag tgtttttttg tgaattaaag aaatgaatga 129000attcacaatg tatgacttgg gccttaagga agatggcatt cctacatctt aaaattagta 129060aacagcatac ctttaccagg cacatgttga tcagaaataa aggaaagtaa gtagcctgta 129120gaactggggt gctcaaacac caatttttct caggattact gaaaaactta aatatacagg 129180ccaggtgtgg tggctcacgc ctgcaatccc aacattttgg gaggctaagg caggccgatt 129240tcttgagctc gagtttgaga acagcctggg caacatggcg aaaacccatc tccacaaaaa 129300acactaaaaa tattagctag gcatagtggc atgcatatgt agtttcagct acttgggagg 129360ctgaggcagg agaatcgctt gagcctggga ggtggaggtt gcagtgagct gagatgacac 129420cactgcattc cagcctggag tcagatcctg tctcaaacaa caacaacagt aaaaacaaaa 129480caaacagaca aacaaccttt aagttataca gctaaatata aagttcaccc caaacttagt 129540aaatctgaat ctgcaggcat tcaggcaaaa ctgcagactg agcgtctgat tttttttgtt 129600ttgttttatt ttgttttgag acggagtttt actcctgtta cccaggctag agggtagtgg 129660cacaatctca gctcactgca acctctgcct cccgagttca agcaattctc ctgcctcagc 129720ctgtggagta gctgggatta taggcacgca ccaccacacc cagctaattt tgtattttta 129780gtaaagatgg ggtttctgca tgttggtcag gctggtctca aactccagat ctcaggtgat 129840tcgcccgccc tagcctccca aagtgctggg attacaggca tgagccacca tgcctggcca 129900tgcatctgaa ttttttaaag gtgccataag tgatatacag ccaggtggtt actataaaat 129960gtttgcagat aactggcact ggttgctatg aagaagcaaa tatttttaaa tttaataata 130020ttaagaattg tttgacccag gagtgcaaaa gcagcctggg caacaaagcg agaccctgtc 130080tctacaaaaa ttaaaattag aaaatcagcc atgcatggtg gtcccagcta ctctggaggc 130140tgaggtggga ggattgcttg ggcccaggtg gtccatcctg cagtgagcta tgagtgcacc 130200actgcactcc agcctgggtg acagagggag accctgtctc agggagaaaa aaagagaaag 130260cacagagcct agtttgttat atcacccttg ggggttcaag gtcagcacca tggacatcgc 130320cattggatgg gttggttttt atcattcctt ccttcacacc agtctgtcaa ttaccccatc 130380ttcctttatt tcgttgctaa tatttatgcc tctctagtgt catcccactt gatgtattta 130440atatgttgtt ggcaagcatg gcagttagct caacttttta gcagctgtat tttgaatgaa 130500aaccaacctc atgttccaag tcctcttgct taattctagc aaattcctaa accgtatcca 130560aatctagaag tctgacccct atgtggggtg gttcttagga aaacttattt agactgtatt 130620acaagtagtt cggttctaac ctcttaattt tacatttgta caaattgagg ctcagagaga 130680tgacgttctt gagatggaga gttactgata ataaactaag ttcaacagtg ttttcatggt 130740ttggtccaga agttactata aaatcgtaac cacatccatg gaaggaatat ctagttacta 130800ataaaaataa atctaatggt tgatacagtt tctctaaatc ctccctactc tttattaaaa 130860cctatttaac cacatttata catgtttatg tacaatgtta tccaccccaa taagtcctgg 130920gatcttgagg ttaagagtcc tggttctatt catctttgaa tacaccccaa caacaaaaac 130980agagattaat tatactcata ttttttcttt atcatgctat aaatccactt tgcagttaat 131040atctatgaaa attaatgaac atttaaaata aagtattcaa cttaggaatt gaatatatat 131100tatatattta ttgaatgcgt tttcatctct atttaaggaa accagataat tgcactagcc 131160aaaataaaaa aatttaaaat ccacattaaa aagaaatgta ttcagggcca ggcatggtgc 131220ctcatgcatg taatcccagc actttgggag gctgaggctg gaggatcacc tgaggtctgg 131280agtttgaggt tggcctggcc aacatgtata taatatatat attgtatata atgtatagta 131340tatatcatat agactgtgat atattgcata tattatatat agactatgat atacagcatg 131400tgttacatat agtatataat atatggtgta tattatatgt ggtatatatt atatagagac 131460tgatatactg tattgtatat attacatatg atatactgga tagactatga tatactgtgt 131520atattatata tactatttac gttattctgt atatattata tatacactat atctctcacc 131580tttctatcta aacctgcttc agttgcattg catggagcct ttgcatacag tttgttcacc 131640cctaaattat acatgcagtt caccagatcc tattacgcta tgtctgttcc ccctttaaat 131700cgatttttca caagcactta gcttggtgtc ttcagcgtct ggcacctggt aggcattgag 131760atgtttgttg caatctttct caaatgctgg gtgtggatgg tggtggtgtc agcagcagaa 131820ttaaaagata aaggaaacca tctgtcctta aaggaactca ccctggtcac agagactgac 131880atgcaagggg aaagggtatg aatgtatgag ccaagcatgc cagtgtccgt tctcctggtt 131940cccaacaaca gtgttaattt tatggtagat gccatttcaa gtcccacagc catcgatgtg 132000gtcaaatgaa aggtgttaca cagggccggg tgcggtgggt cacacctgta atcccagact 132060tttgggaggc caaggtgggt ggatcacctt aggttaggag ttcaagacca gcctggccaa 132120catggtgaaa ccccatctct actaaaaata caaaaaatta gctgggcatg gtggcgggtg 132180cctgtaatcc cagctactcg ggaggctgag gcaggagaat agcttgaacc caggaggcgg 132240aggttgcatt gagccaagat catgccacag cactccagcc tgggcaacaa gagtgaaact 132300ccatctcaga aagaaagaaa ggaagaaagg tattgaacag aattgaaaag tttgtcagat 132360gagtgtttct ggaagacctt aaaattaaga aattatttgt tcataataaa gactaccctt 132420ttccctacag aatgtaatta agatatttat tttttataac ctaaaaattc gtctttgtca 132480gtatcatttt ctgcaagata ggaagctatt atcctgcagc cactggtttt tgtgaaactg 132540cccatggttt acgcctttgt atttttttcc tgtgtcctct ggagcactag ttagccaaag 132600tctaaaattg gagcagaaat tatggggcag tggagttgga attacagctt agactcctgt 132660ctctaccaca tgtaactatg gcctgccatc tgatgttctt catttgtaaa gtgggaataa 132720tagtacttaa tatacctgtt gaaaatattt gactttctga ctcagaaggg atcttaaaaa 132780tgtcctttta taattgaggt aaagaagaaa tcgctaggta gggaaatgta tgtagtaaat 132840gatgaaatct gtaaagatgt tttaaaaatt gtaaatacct ctacaaatat catatactac 132900tcaagcaaca tcctaacatc actaggcatt tgttttctgt ttaactgcct ttggaagacc 132960tgcatgtaaa gtaacaaata tgtctcaccc cagacgtgtt caactcccgt atctcccttt 133020ttcattacag tcatctctct ggctccttca ctgcccccat tcctatccat ctgctctttg 133080atgtcatatg caccttacag gtttcagttt ctcttcccgg ctttgctttc ttttctgctt 133140gtcttgagcc ccatggttga ctagttgaag cattttcgaa taaggagttt caattccttt 133200aatatccggc ccacttttcc tccttttttt ttcttttttt tttttttttt gacatggagt 133260ctcgctctgt cacccaggct ggaatgcagt gctgggatct cggctcactg caacctctac 133320ctcctgggtt caagtgattc ttttgcctca gcctctcgag tagctgggac tacaggtgca 133380cgccaccata catggctaat ttttgtattt ttagtacaga tggggtttca ccatgttggc 133440caggctggtc ttgaattcct gacctcaggt gatccgcccg ccttggcctc ccaaagtgct 133500gggattacag gcatgagcca ctgtgcctgg cccccagccc atttttctac gcttcttgcc 133560taccttggtt ccatgataaa agccacctcc ttagcctgtt aacccataca atggaggttt 133620agggagtggg tggaagagct ttagtgtctt tggtttaaat ttaggtctgt tttaaaattg 133680tgtctgatac aaccacatct ccttaataaa cgtattggtt tactattttc agttcagtct 133740ttagaaaaca agcagccttt caggaagaat ttcagagagg tttggttctc aaattttgtg 133800tgcttctaaa tattctttat accttgcttc aaaatgtagc tacctgagct cacctttctg 133860tagccatttc agtttattag gtttggggtg aaatgtgcca tttgtatttt caacaagccc 133920caccccagct gctgaaaatg atgcaaaatc aaatttgagc atcgctgctg tagaatacat 133980tctctctttc ttccaatgga gagttccatt cctgaatgtg ggatatgttg aaatggtgca 134040gccaaatttg cattgttgtt cggtaatatc agtggttctt aatcaaagac atgtatgaga 134100atcaactgtg gtgcttttga aggtcatcac taaaaattgt ccacgcttta gctccaccca 134160ctggcaattc tgtgaaatac ttttcaggtg gggccccgct cgcctgttaa cgtatcacag 134220gtgatttcta cttggtataa tatggtatga cccatctgca gtggaaacaa atgcctgttt 134280tggataggag tatttcaatt caagttaata tgaattgata aatacacagg taatatttgg 134340gttgttattc aaagaactta taaaagtcat atcttgtaaa agtctataga tttattacag 134400ccttttaaaa aatattgcca ggccccaagc cctgaaaata tgttgcgctt gctccttccc 134460ttactgaaat cacatttaaa ttcagaatca ccagtgctag tttactttca gttcaagaca 134520gcaagcctga aaatagcctt aatgacgcat gcagagcatt ttcctaggaa cagactctgg 134580acttattagt gtctggagcc atcagtcaat acttttctgg tgcactggtg tgttagtaag 134640ggatgccaga aatgcgtgcc ttctcttatc agtcacccgg agaattcacg taacttgctt 134700taaatagccg aagtgaagaa ggagattgac tttacagttg tgtttgcttc tttaaaaaat 134760atacttatct tttcctagct gtaaaatagc aatcgaaaac tgccatctaa agtgaaaaat 134820aattccctaa ggtttcaata gggtttttct tgtatcagaa ccagggccag ggtgtaactg 134880gatttaagtg ccagacaagg agctagaagc tgcaggtgca gctccccgcc acgctgctct 134940ctgtgacctt gagtgagaca caaaattgct gggcttccgt ttttcatttt aaaatgcgag 135000ggttggattt gctcactaag agctctttta tctctaatag ttaagatgat ggaacatgat 135060gttttgagat gttttgctga aatttctcac atagaaaatg ggccttctga aaacatataa 135120ttatagtttt gggtaggaag aagctgtcat ggtggcgaag gggaacattg caaatctgaa 135180catgaccgca gctggcggga gaataatgtg ttagaattga aaggcaccct gcagacttgc 135240tgtttaatgt acaaagaagc ctagggccgg aggggcaggg ggactctcag ggacagtgga 135300atctggctca acggtcaccc tctggtagca agcaagacct gtctctgtga caggtctcaa 135360tcaatggaga agcttatttt gccgacgtta aggacatgcc taggctcagc gtcctaaact 135420aagccaagtg caccaagcca ggtgctcagg ctcctaaact aaggtgcaat taggcaatca 135480gcatggattg ttagactgta caaaggtgga gatgccaatt ccccaactgt tttcctaggg 135540gttgtgttcc cacacacgca ctttctcctt agtaacagcg cacggctcct gacctggatg 135600ggtggcctta gatctgtgaa gcacatctac cttgccttga gaaagatgca actcaatgtt 135660ggcttcctta gctccgtgcc actcagcatt gcagcaccga aaggaaaact gtttcctctc 135720tactctccca ctactctcaa ttcttccaac aacagatgtg ggttgttttc ccccccaaca 135780gcaagcagtt ctccagttct ctgcagatgc taatgccacg tcctgcaatt caattctgac 135840cttatctacc tggagttagt gcagacccaa caggttgaag gctgagtctc aaaagactgt 135900ccccacttca gatgccagtc gcacaagtga tgagtctcta ggttatcctc aacttctgtc 135960caactcaact acagatcaga ggttcccatg gccccctact caggttctat aatttgctag 136020aagggctcat aaaactcaag agaacagccg acttactaga tgactagttt attataagag 136080gatacaactc aggaacagcc agaggagaga gggatgggag agggtgcaga acatccatgc 136140catatctagg tacgccaccc ttccagcacg gccctggggt cacacacctg gaagctctca 136200gatctccatc cttttgggtt tttatggaag ctttgttact taggcaccat tgactaaatc 136260actggccact ggcgattaag tcagtctcca acccttttcc tgtctctaga ggtcagggtg 136320ttggggcgga aagttcaaac actttaatat tacggttggt tcctctggca cccaggcccc 136380atcccaaggc tctccaggag accccagcca ccagtcattc attagcatgc caaaagacac 136440ttaacacttt ggagagtata agagttttag gagctgcatt ccagtaaaca ggtgtgcaga 136500tcagataagc atttcttatt gtattgcagt atcagagatg atgctcaccg ctggaccaca 136560ttccaagata tggctccact tattgatctt tgaatgaagg ggtaccttat tgatcctccc 136620cctgacctta ccccaaataa caaatcatac cagtactaaa ctggcagaac tcaagattgg 136680ttaggaaaga tcctctgaaa cttcgtataa tgacagttac cttgttttga gtgtgtcccc 136740tgtgccaggc actgtgctga gagctttgtg tgttcagact cacttcacag ttacaaaacc 136800ctctttaata tgtgggaact gggcttgaaa tggtttaggt acacggtcca gttcatagcc 136860aatgttgtgt tactagaaag ggatcccaat acagaccctg agagtgggtt cttggacctt 136920gtgcaaggaa gaattcaggg caaggccata gagtaaggtg aaagcaattt gttagagaag 136980ttaaaaaaag aaaaaagaat ggctactcca taggcagagc agcagcatgg gctgcttaac 137040tgaatatact tatagttatt tcttgattat atactaaaca agcagtggat tgttcatgag 137100ttttccagga aaggtgtggg cagttcctga aactgagggt tcctccccct tttagactat 137160atagggtaac ttcctgacat tgccatggca tctataaact gtcctggcac tggtaggagt 137220gtcttttaac atgttaatgc attgtaatta gtgtattatt gttacagtgg agggtgtcca 137280cgttcttggc gtcttcaaca aagaattgga caaaacccac aaagcaagga aagaatgaag 137340caacaaaagc agagatttat tgaaaacaaa gtatactcta catagagtga gagcagccaa 137400agcaagcagc tcaagagccc ggttacagaa ttttgtgggg tttacgtacc cctctagagg 137460tttcctgtgt ttgcttggta tacacgctat gtaaatgaag tagcgactct ccatcagtct 137520gattggttgt aggaggagac taatcagagg ctgaagcgat gttatgaagt tgcaccctat 137580gcacacatct gattggctac agaaagtgac caatcagatg ctgaagtgaa gttacaaagt 137640tatacccctt tgtaaatgaa aacttggccc cagaccagcc tgaatggttg caggagggtt 137700ctaaacagag gtactttcaa tttcagaggc agagcatggt ggcttatgcc tgtaatctca 137760gcacttaagg aggctgaggc agcccaatca cttgaggtca ggagttctag accagtctgg 137820ccaacatggt gaaactccgt ctctaccaaa aatatttatt taaaaaacta gccatgtgta 137880gtggcacaca cttgtaatcc cagctactca ggaggctgag acaggagaat cacttgaacc 137940cgggaggcag agatagcagt gagccaagat tgtgccactg cactccagtc tgggtgacag 138000agtgagattg aatctcaaaa aaaaaaaaaa atacagttct catctgccac acagaaaaag 138060gagggggagg gtattgcaaa gggcgtagcc tttggtcctt ttgttatttg agcgtggaaa 138120gttggggttt ttcttttgat ttagttttag gaagtcagcg tgaatcagcc ttaggttccc 138180tgtctccagg ccctattctc ctgcctcata ataaataata ataataaaca gggaagtcga 138240ccagagatca ctttcatcac catcttgatt taggtgggat ttggccggct tctttactgc 138300atcttgtttt atcagcaagg tctttgagac ctgtatctgg tgctgaactc ccatgaataa 138360gaatgcctaa actcttggga atgcagccca gtaggtctca gccttatttt atccagcccc 138420tattcaaaat ggagttgctg ttgttagaat gtctctgaca gctggactca tccaaagcct 138480ttttagtaat gccatgactg tgtaaatcca gaagtctctg tgacaggtct caatcaattt 138540agaagctcac tttgccaaga ttaaggatat gcccaggaga aaagaacatg gaatcactaa 138600aacagtctgt ggtctgggcc tttctccaaa ggtgaatttg agggcttcag tatttaaagg 138660ggaagagtgg gctgcagagg gaagatggag ggtgtggtaa ttcacatatt gcaggaggaa 138720aggggcaggt tagggaagag tcacttatat attcctccgg ctttcagtaa attggcactt 138780ttcaaagata agatggacac agagtaggaa gtatttaacc ttttagttgt agttgtctgc 138840ttaggaacaa aagggaaggc aacttcttgc atggctcagc tttcagcttc attttttttt 138900cttttggcag agtgaattgg ggttccaagg tttgtttgtt cgtttgtttg agacggagtt 138960tcactcttgt tgcccaggct ggagtgcaaa ggcgccatct cggctcactg gaagcacctc 139020ctgggttcaa gtgattctcc tgcctcagcc tcgcgggtag ctgggaatac aggcagcggc 139080caccacgctt ggctaatttt tatattttta gtagaaacgg gatttcacca tgttgcccag 139140gatggtctca aactcctgac ctaagttgat cctcccacct cagcctccga aggtgctggg 139200attacaggcg tgagccacca tgcctggccg gagtttttat tttcctttta caagtgcttc 139260ctcttctatt tcccacagaa cattctggaa aaccaccagg gtagaaccct gacttctatt 139320tgaccacagc tcaccattct tatatgtttt gtgattttca ttttccctta agaattgtct 139380ccaattgcga cattcttcag aacttattaa agatatctga agtgtatacg gtgaaaaatg 139440tatagaatgg tggtgtcacc acttgatgtt gagtgtgaaa tgtgtgggta gtaaacttct 139500gaagctgctg ctgtaagggc caagggaaac ttccccttca ccctgtgaaa gtttgcagaa 139560aaagcaactc actaagacag gttaattgga gaaaaggcat gcaaatgtat ttaacatgca 139620cacggggaga atctcagatt acttattccc taatagagtt aagaagctta tacagcatcc 139680tggccaaaca agttatggga gtagggagaa gaggaattcc attgagagga tcactaggga 139740gaatgaatgg atccaggaac agagataaat ttgtaaatag tctcttgaca gttaaggttt 139800ggttgcattc ttggtattaa cagggagggg aagcagaaac cattggtctc tttggtgcct 139860ctgggtcctg ggcagataca gcctttgctg tgggagggat gtgggggacc ttgaggcggc 139920ttcttcattt cagcaggcca actactcagg aggctgagtc gggaggatcg ctctaagccc 139980agtagttcaa ggttgcggtg agttctgatc ctaccactgc actccagtct gggtgacaaa 140040gtgagacccc gtatctaatt aaaaaaaaga agacacctct tcaaattttg acagagttta 140100actctttcat caaaatacct atatatttta tgtaatatgg atcatttata tatacacata 140160tctgtgtgta catattgtta tctgtatacg tatatctgta ttatataaca tatataatct 140220gtgtgtatat gtatatatct ttttatgtgt ctatccttcc tgacacccat tccctgaatt 140280cttttctgcc aagataaatg caaagagcca gaaaatctgt gatcctatct cttaacagat 140340ctgtgctgag aaattactgt aggtatacct ggagttctta ataaaaattc tagttagatg 140400acttttgaga accataaaaa aaaacttaag agaacttctg ggatctctag tagggactaa 140460ctttctcttt acaaatgttg acaaattgtt gagattgtct gcggagatag tacatgatat 140520ggtttggctc tgtgtcccca cccaaatctc acattgaatg gtaatcccca tgtatcaaag 140580gaggggcctg gtgggaggtg attggatcat gggggtggat ttcccccttt ctgttctcat 140640gatagtgagg ttcttatgag atctgatggt ttaaaagtgc gtgtgtctct ctctctcgct 140700cgctcgctct ctcgctctca ctctctcttg ctctcctgtg ataagacatg cttgcttccc 140760ctttggtcat gattgtaagt ttcctgaggc ctcctgttaa gcctgaggaa gtgaatcaac 140820taaacctctt cataaattgc ccagtctcag gtagttcttt atagcagtgt gagaacagac 140880taatacagta tatgaggtac acaagacagc tcattacttt tagagtcaag cattgttttt 140940aatttaaaaa tgttatctcc caggttgatc ataggctgta ttcattcagc ttggctttga 141000gtgactctgg gctgtttgct aaaactaatt tgtcctcaaa ggaaagcgag ttatgaatag 141060gggcattcaa aattttatga ctttggttct gatgatactt tcaaaaaatg aattttaaat 141120aattttcaaa tgagagaaac aatcctcaaa tatatgttca tttttttcca agggtttttt 141180gaaggggttc ccattcactt ggataggttt gtatatgctt gacatttttt aattactcaa 141240attatttgag gatcacaatt cacttttaat tgttttttta atattggaga tgtgggattt 141300gtttttttct cttttggttg tcaacctggt tactaacaga ctgattctcc ttgagcatcc 141360agaataagac aagcccgttg gtttaatagg ttatctgtgg taagtaccgt catacacagt 141420gtgtctcctt tgtaaaactt aagtgcaatg aatgatgttg ttactagtcc aacgtgtnnn 141480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 141960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 142980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 143940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc ttatctctta ttggttttta 144000cagagactaa ctgagatata tttaagcaac aggacttcta catagtaagc acaatatagt 144060tgcaaattaa ggcagttatt aattattaca gtctaataat gtctgaaaaa aacaagtctg 144120tttatcacgc tgaatcacat ctgtatactg ccacaggtat acctcaggga agttcagatt 144180actacccaag ggccaaccgc tgcttctgaa acatcaactg acagaagaac ctacggaagt 144240tataatatct cctggctaga ctgaagatgc cttgagcaga tatttgggtg tttttttctg 144300ttttttgttt gttttgtttg ttttgagatg cagtctcgct ctgtcgccta ggctggagtg 144360tagtggcatg atcacggttc actgcaacct ccacctcccg ggttcaagcg attctcctgc 144420ctcaacctcc tgggtaggtg ggagtactgg tgcccgccac catgcccagc taattttttg 144480tatttttagt agagacaggg tttcaccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 144960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 145980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 146940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147420nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 147960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 148020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 148080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 148140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 148200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 148260nnnnnnnnnn nnnnnnnnnn nnctgtccac taatatcctt cccagccctg ccagattagt 148320tcttgcgtat acccatctgt taatgacagg gtccaggcat ctgtctctag gcagaaaaga 148380gcatacggca gtgagatgag ggacttcaag gccaggggat agagggcatg agtcagggca 148440gaagcatgga gaaggtggga aatttgggga gcttgaacag atgtttctgg accatccttt 148500tgggtctgta ggccagatag taatgaagca ttattgtgtg gggtaaatat gtgaggttca 148560ttgtctcacg gcagggaaat cgaggacgtg gacgtggaag aaatgagttt aagagcggag 148620gtttaatagg tgaaagaaaa agagaatagc tctctctctc tcttgcaaag agagaagggc 148680tcccaagtgc gtcatctggt ttcatggtga aacgcatggg gtttcatagt tgagcttgag 148740aaggcgctgt ctgctttaca tagggcgcga gagattgttc ggaccaggtg tgacatttgc 148800atggtgcatg aagatgctgg ccagcccacc ctaatctttc atgcagatgg ggtctcaacc 148860tggccagcac catgttgtct gttcctcact gtacttgtgg ttgacaaaca aaagggaaga 148920tggagccacc aggttgcaca tgcctggccc ccaggtagcc tttatctatt ggcacagctg 148980cggcatttac ctgtgcagac ttttagcttg tttatctatg ctttctgctt gatnnnnnnn 149040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 149940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150420nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 150960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 151020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 151080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 151140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 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nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 153720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 153780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 153840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 153900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 153960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 154980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 155940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156420nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 156960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 157980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 158820gagaaataat agaagaaaaa gtcattgctt tcataattcg ttgcagccat gaatgagagt 158880tgctatagtt gtagcatatt ttccccggct cttttctcat tcaatgattg tacctttcgg 158940aattatgtct ttaatgatta attaattaaa gtagatgttt ggggtagcaa ggcagtgact 159000aagtcctttc cactgaaacg gaagaactgg ggattttatt catgtttgtg tttcctgatt 159060tacagttgct acaagcgcca atgttgaaaa cacatatttc cgtggctctt cttcaccttc 159120attacttgta gtgctttcaa agagaacaat ggcctttttc tagttcagaa atgggtgtta 159180tgtagctcac agctgggaga tttcaagtgt tttgaatacg aacaagctgt tttgtgataa 159240agaaccagtg cagaggggaa accaacaatg attacagtag ttattcagta tgtctttgtg 159300tttgagggaa aaaggaggga cagtgttgtt ttgctcttat ttttttccct cttccttttc 159360ccactttaga caaagcccgt tgcatttgcg gttcggacaa atgtcagcta cagtgcggcc 159420catgaagatg atgttccagt gcctggcatg gccatctcat tcgaagcaaa agattttctg 159480catgttaagg aagtaaggag aataatttca ttttctaaca gcatgatgtt tcaccttgac 159540ataccatttc ttatttccta ttcatatgcc gtttctgtga agtagcattg cacatcgctg 159600cagttgtata acacacataa ctaaatcaat ggctctcaac ctctgctaat ataaatatac 159660cttttaaagt ggaaagaagt cacaggcctc ttgcaatnnn nnnnnnnnnn nnnnnnnnnn 159720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 159780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 159840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 159900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 159960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 160020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 160080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aatttatact 160140tggagctctt cccatctagg gtatatataa tatgtcatag gaagtatgtg taaatcgaaa 160200cttttcactg cactaaaggc tgtcagcaaa ggagttgata actgccgatt aaaagaacct 160260agggctgggc ccggtggctc atgcctgtaa tcccaacact ttgggaggcc aaggtgggcg 160320gatcacaagg tcaggagatg aagaccatcc tggccaacat ggtgaacccc gtctccacta 160380aaaatacaaa aattatctgg gtgtagtggc acatgcctgt aatctcagct actcgagagg 160440ctgaggcaga agaatagctt gaaccagcga gtcggaggtt gcagtgagcc gagattgcgc 160500cactgcaccc gagcctggcg acagagtgag attctgtctc aaaaaaaaaa aaaaaaaaaa 160560aaaaagaaga acctagaagc ctgggtacta tggctcatgc ctgtaatcca agcactaaga 160620acctagaagc ctgggtacta tggctcatgc ttccaagcat tttggaaggc cgaggaaggc 160680agatctcttg agcccaggag accagcctgg gcaacagggt aaaaccccat ctctaaaaaa 160740aaaaaaaaaa aaaaaaatta acctggtgtg gtagcataca ccggtagtcc tagctactca 160800ggaggccagt ggattgattg agcctggaaa ttcaagacag gagtgagcca ggatcatgtc 160860actgcactcc agcctgggcg acagagcaag accctgtctt gattaaaaat agaagagcct 160920agagaattta gagcctacca ttttggtaac ctctgaaaag agatgtcaaa tactggctag 160980gtgggaacaa tagaaaaagt tatcagctgt gcctagaatc tcactttaca ccaggaagtt 161040agattattat gaggtctctt cttgatgatt gatgccgaaa gggggatctt tccaccatga 161100aaggaaaaga aaaacagaat gtatattctt cttcgacgtg ttcaaggaca gatttattca 161160atcgaggaaa aagaaggcaa atcacaaaga tttaagatac atattccacc tgcaagacat 161220aaattgtcaa catcacacaa gtagagaaat catctgtgaa catcgcactg tatatttata 161280aaactgtttg cacaaaggta aattgtcctg tgacatgaaa tatttaaggt acacttcaat 161340atttatctct ttctctcaat aattccaatt ggtctgatta tataagggga agatttctgt 161400aacagtactt cctaaagatc tcttggtagg atgggctgtt attctttgaa cccagagagc 161460ttatccctac tcatggtgac agcagctgct gcaagggaat tcagcctggt cctacggcag 161520caccaggaca gacaaggaac gcagagcctt tgatagattg ctgaggacgc taaatgcatt 161580tcttacatgt gaaacccatg agccgaaggg tttcttgaat gataatattt aaggcatagt 161640taatggtcat tgccatattt ctctcgactg aaaatagtgt ggaagaacaa ggatttgacc 161700ttctgtgcac tgatttttaa tgcttttgat tttgtgttta gaaatttaac aatgactggt 161760ggatagggcg attggtaaaa gaaggctgtg aaatcggatt cattccaagc ccagtcaaac 161820tagaaaacat gaggctgcag catgaacaga gagccaagca agggaaattc tactccaggt 161880atgagacaga tgtcaagtgt ttgcataaaa cttagattat accactatct gtgtactgtt 161940gtctgctgta ttctgtatcc tttattatgt attaacaagg aggctggtaa tatggtttat 162000tgatagcatc acaaatcttg cattttcctg catttaaaaa acttcagtcc agcacctgat 162060tttcacccta tttgggaaag tgtcaaatta ttatcattaa tgaaaagagc taaggtcata 162120gagtattaaa ttcaggctcc agtgcagagg aaaatctgaa accttaatca tttactgaaa 162180aatagtctgg cacatcatag ccctggtgac taatcccact gtctgtagct gcctggtatg 162240gctgggagat gtttgagaga tgacattctc agacatcatc aggcagtggc agctcttgtc 162300atccgttagt ttcattttca cctgagaaac tcaattttca gttctaccca ctgttaagca 162360cttaaagaga agcgaagtat gaaacaagtt aaaactgcct gtgcagatgg caattagatg 162420agttatgtaa gaagctgggg ctgaataatc cctcctagga tttgtgaggt tgtctgtccc 162480tctctgaaag aatcactaga aagacttctc gtagatgtac tgaattttcc atggaatcgt 162540atgcagacgg gtctaaggac gcagcatgcc tctcccaatg tgaaaggatg ggacatgggc 162600aaggcctttc ttaaatacga atcgctggat ttctcttttc tccatcttac atcttatttg 162660tttatctaag atgcaaatcc ctttgtcagt gataatactt tactgtgagc aagtattttt 162720ttcaagcatc agcagatgag tgaaacagaa tatgctttgt ggtttggaac acccatcctc 162780attaactggc ttttcataat tgtcaagttc atttagttca gacggattcc tctgtgtttt 162840gagacctcca ccccagaact gactttcatt aattcctggg ttattgagag cactcagatg 162900gtgagatgca ttcagtcata taaaagtaga ggcatgcata aaaaagaaca aagattggcc 162960gggcatggtg gctcatgcct ataatcccag cactgggagg ccgaggcaga cagatcacct 163020gaggtcagga gttcaagacg agcctggcca acatggtgaa accccatctc tactaaaaat 163080ccaaaaatta gctgggcatg gtggcgggag cctgtaatcc tagctactcg ggaggctgag 163140gcaggagaat tacttgaatc tgggaggtgg aagttgcagt gagcccagat tcgtgccact 163200gcattccagc ctgggcgaca gagtgagact ctatctcaaa aaaaaaaaga acaagatcat 163260gtcttttgca gcaacatgga tggagccgga ggccattatc ccaagcaaac taacacagga 163320atagaaaagc aaatacgggg gccacgtgcg gtggctcaca cctgtaatcc cagcactttg 163380ggaggttgag gcgggcatat cacaaggtta agagattgag accatcctgg gcaacattgt 163440gaaaccccgt ctctactaaa aaaaaaaaaa aattagctgg gtnnnnnnnn nnnnnnnnnn 163500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 163980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 164820nnntagtatt tactaaaatt gctttctcat aaaaactccc ctggttcatg tcttaggaat 164880agtgttttgt agaatacatt ttggaaaatt tgggatggat ttttgcagag gcaggcttgt 164940gtgtctttct ttgcctcttc atatagaaac tgagaaatga gaacaacttc aaagggagta 165000gaaatctttc ccaaaattgt ttgagaaatg tagtctcctg tgtctcccaa atgggcttgc 165060ctaggctaac attagtgctc cactcatctt tgtaaggacc tgtgggagca gtgggtatta 165120gatcttacag gtcacagatg agaaaacagg ttaagagaag ttagggaact tgatcgaggt 165180cgcagagcta gtaagcgtca gaaccaagat ggtagcctga cctccacgct gtacagactt 165240ccatgctgca ctgcagatta aggagccagg tcttgcctgg cacagcaagc ccaggacaga 165300tggttattgc catctgttat tatttacttt attagatatg ttgttttgct cttgcatttt 165360ttcccttagg actctgagtt atccgtattt gagggaatgt aacatctcag aacttactcc 165420agagaactag attacctttt cttacagcca tatatctttt cctttgattt tcttcatttt 165480attttggccc aagattttgc ctaagtatgt gaaaatactt ttacttgctt tcataggttt 165540ttacatttct ctccttttgt gtgtgtgtgt gcgtgtgtgt gagacagagc ctcgctctgt 165600cacccaggct ggagtgcagt ggcgcagtct cgactcactg caacctccgc ctccccggtt 165660caagcgattc tcctgcttca gcctcccaag tagctgggac tacaggcgtg caccaccacg 165720cccagctaat ttttgtattt ttagtagaga cagggtttca ctgtgttggc caggctggtc 165780tcaaactccc aaccttaggt gatccaccca ccttggccta ccagagtgtt gggattacag 165840gcgtgagcca ccgtgcccgg cctctcctaa cattttaaat gtagttttgt tttgaggaca 165900taaccagaat ccggcagtct ttaaaaactc tgatacagaa attagaaaaa ttgacagaac 165960tcaatggaaa tggaattggg gtaccgttca agaacttggc attttaatag aattaaaagt 166020caaggacaaa tctgcatcct ggtcattgtc accatttgca gtatttgtca caattttaca 166080taaataatat tttggctgtt actaaaattc aaagggaata taaaacccat gaacttaaac 166140tgaaattaga cttaatactt cagaaaaaaa aaaaaaactt ctggggaaaa aaatgttttg 166200gtcttggtaa taggtccttg tataactgcc agaaactgct tctatagata tcacttgtca 166260ttaatttcac atactgtctg atgcccttat taaatgtatt tgagtatctc ttaagaacct 166320taaaaagata tacagccctc ctgctaattg cctaataaag tttttgatgt ttatgcatta 166380aaatgctaga acagtagcaa taacattcag cctgttactg atactttaat aaaactttta 166440tttccatatt aatatttacg taagtaaata ttgccctttg atgcataaac actgtagcaa 166500attctttcaa ctagaccctg gctcggccat aataataaac attaggcagt gagatcattt 166560tgcccaaatg taaaatgttc ttcttatctt catctgagag aggggtcatt cggtaacacg 166620ttatttagca tgctagtagg ctggtgaaaa acacaaatgc tgttgttcta atattaactc 166680caatgtttct gagacactgc agtatatcat atcaaacttt caaatttggt agttcggaaa 166740tgtatgatct tcaaattatt cctaaattaa ccacgtttca ttctccacgt gagtcacgct 166800tgagaagtat tctgggtact atctgatggc tacatcatat catattctgt ttagtcatgt 166860ttcttcgtgt gtccgttagt ggttggcaag ttttcatatg gctctgttgt tctttgatag 166920attgctcatg aaagcgtgag tgctttcaat cttagtgtac tattaataaa ataagcctaa 166980aacagaggag ctgaaaccaa acaagaagga aatattaatc ttgggtgtta gccttaaggt 167040tctatgcatc gcctctgaag ggtctctaaa ataaaaagca aaataaatct gtgctatttc 167100actgtctgtg atctgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 167160nnnnnnntgc gaaataagtg tcagatttaa tagaaatttt tgctttactc cagtaaatca 167220ggaggaaatt catcatccag tttgggtgac atagtaccta gttccagaaa atcaacacct 167280ccatcatctg gtaagtaggt gataaatgct gaataataca tactgcattt catgctttcc 167340ccagctctat ccagttttgt gaatttacgt atacactgaa tcactatgtt aaccctacag 167400atgttaaaaa gaatcagaag tgagcatgcc cttttggagt tacaaacaat atggggaaat 167460gtttttttct ggctggaaaa agaagtatgt caaagtgttt acattgctgt agatgatcaa 167520aatgattaat gtatgtattt atttatttat ttattttttt gagacagagt ctcactctat 167580tgcccacgct ggagtgcagt ggcatgatcg cagctcactg cgcctctgcc tccagggctc 167640aagtgatccc cccacctctg cttcccaagt agctgggact acaggtgcac accaccatgc 167700ccggctaatt tttgtatttt ttgtagagat ggagtttcac catgttgccc aggctggtct 167760cgaactcctg agttcaagcg atccacctgc cttaggctcc caaagtgctg ggtttacaga 167820tgtaagccac tgcacccagc catattaacc tttaatccct agttcctgcg atggtgcttg 167880tcatgggcag taaatgggtg aatacttgcc aacaaccata gcacaatgtg agatataacc 167940ttaacattaa acagaaggat aaaaacttta gaaaggtaag gaacagaggg taaagtgaag 168000atagaggatt ggcctgttgt gggctctacc agaaaagttc tgtggaagac acagacaaga 168060agtatagaaa aaagatgaaa atgtgtgtgg gaacagtaac tactgttttc tttccaaagc 168120agtgggttca tcctaatggt tgctggataa aataaagcac acctacttaa atttgaattt 168180cagataaaca ataatttact agtttaagta catattgcat ggggcatact tacactaaaa 168240atgtatggaa catacttatg ctaaaacatt gttttaaatt caaatttaac tgcatttata 168300tttgctaaat ctgggaactc tcctattctg tgtagaacat ggaattctat atagacagga 168360gactaatgat ttgaattgca aaaaaaagca catgattagt atcttagttt gctaagagaa 168420ttgttattgg gaatcagagt aagactgtaa aggttgactg ccagggtgtt tcagcaaaat 168480gatactgtgt gtgatttcta gaaagctgga aatgggaaga gataaaaaac agagagttgt 168540acaactatgc agaaatcaaa gaactagtca cacagtgact gatgtgcttt aaggacagga 168600tttctctatt actaataatc agcttcattc ctgaatggtg gtggccttta gagaccaatc 168660aaggacttct tagcaaaaac tataggattt ttttttttta agagaagggg tttcactatg 168720ttgcccaggc tgttcttgaa actcctgggt tcaagggatc ctcctcctca gcctcccaaa 168780ctgttgggat gacaggcatg agccaacatg cccagccatt ggcaaactaa gatactgatc 168840atgggctttt tttgcaattc aaaccattgt tctgtttata tagaattcca agttctacac 168900agaatatagg actttcaagt gagcaaaatc tgagcacaat aaatgaattg ctttaggaag 168960caaattaatt tcaaaaacta tctacttata acttgaagca agcaattcta agatgctcaa 169020aattaagtaa aatagtatgc atgaaatgaa aatgaaaggc cctgtaggta ttatgtgaaa 169080cttatacctt cccttgataa ttacggagcc cctgaatcca cagaggaagg gaggcctatc 169140ctgttccttc tcaaatgtgt gaagttgcag ggattcctat ataaacctta cactcttata 169200tatctcaaag atgatttcag tgatttctag ggactgctac taggctctta aagtggaaaa 169260agactatgtc accttgcgat aaagcagcag ttctgcctgc aaatgtcagc ctcacttctt 169320tctatatacc agaaaaattc ttccattccc tccagccata ttgtagccct ccagtggttt 169380tggcaacaca gatgctcccc aacttatgat agagttacac ttcactaaac ccatcataaa 169440tagaaaatat ggtaactcaa aatgtactta acacgtggac agacccagta aaaagtccga 169500cgtcaaacca tcgtaaagtt cagaccttga cttatgataa ggttatgtcc tgaaaaattt 169560gtaataaact tgcacaatct taagttgaac cagtgtatgt caaggaccgt ctgtatagtg 169620aaaggaggtg ctgcattttg aagaacaagt tagtatacat gtaaaataac attccatttt 169680gtaggcagat gtttgacatt actaaaagta aagttccatg aagagtgtaa tatttaaaaa 169740ggtgatttaa atgttcacca aggttttgta gagatacacc aactttcaag tgacttttct 169800ggtgactgag tcacttggag ctataaccaa ctacaaaatg tataataagc atattaggac 169860tctgagacaa tttttatatc aatagctcaa ctgatttaaa ggaaaacttc agaaagctcg 169920aaagagaagt tctcccaatc cacctccagg cacaactgct ccaactatca agtgtggatg 169980ggtctgtaat gatcattttc atcgtttcca aaggtcccat ctattattct cctcactcat 170040tgccagacca aagaatataa gtatctgcct aaagtatata tgcttggatg ctgtcaagaa 170100cttggaaaat ttcccacttg acatctgggc atcggtgtca tagccagttg cagaatacat 170160acatcgcacc cacacacacg tgtgtacata tgtgtcaagc ccaagctctg cttaacttga 170220cccaaacctt taacaccaaa acttaaattt caaaaaattg ttctcggtgc catggtcacg 170280cctgtaatcc cagcactttg ggaggctgag gcaggaggat cacttgagcc caggagttca 170340agaccagcct ggacaatgta agtgagaccc cgtttctaaa aaattagctg ggtatggtgg 170400tgtacaccta tagtcccagc tacttgggag gctgaggtag gaggttcact cgagtccagg 170460aggttgaggc tgcactgagc catgattatg tcactgcact ccagcctggg ccacagaggg 170520agaccctgtc tcaaataaat aaataaacaa acaaacagaa aaaatgttcc caaggctctc 170580tatcactaat aataatatat gcatgctagt gaggaagaat gagacttttc aaatatatga 170640aagcagttct ttccaagtaa atactaatgt ttgctaaagc atggaaatat aagtatgaaa 170700tttaaaaaga gtaacatttc tcattaaaag gagaaccatc tgagtctaat aaaatacatg 170760tagctgaaca gatttgaaaa tgcttcttaa ctgccttggt aagtttcttt aatatgtttt 170820gaaagtgacc acatgtttca gaattctacg tatttcaaaa tgaactcaga tgtttttaga 170880cctgagttaa ccaaatgtca gaactagtac attatagaaa gttctcaatt tgggatatcc 170940agccctcatg tccagataca ggtaaatttc aaacctgact caggctatgt gtgtggaaag 171000ccaaacacct gggcaacgtt gcagccaaag atgtatatgt ttccactctt tctttgtttt 171060tttttgagat ggagtctcac tgtcacccag gctggagtgc agtggcacaa tctcagctca 171120ctgaagcctc catctcccag gttcaagtga ttctcccacc tcagcgtccc aagtagctgg 171180gattacaggc accggccacc acacctggct aatctttgta ttgttagtag agacaggatt 171240ttaccatgtt ggccaggttg gcctcgaatt cctgacctca agtgatccac ccaccatggc 171300ttcccaaact gctgggatta caggcatgag ccaccatgcc cagcctcact cttattttaa 171360aaaacaaaaa tacaactgat gatcacagca ggctagattc aatgggcaaa tcacaaaatt 171420caactcatga acttgactgt ccaagggtcc tgtaaattct gacatccaac tggttatctt 171480cattttagga gagccctcaa agggtactat cccttgaatg tggtctttat gagcccaata 171540aaagatgaga aagataaaac ctgtgggaat ttaccaccag caaggaaact cttgattttg 171600gttaacatgt attgtaggaa taatgaattc ctgacgcaga ttttgtcctt catatccagg 171660aaagcgctga caacatgctg cttgagttga gattcaagga aaaccatttc tgaagagttt 171720tctgagactc ttgcctggct tatgccattc aataagcccc ataaggaaac tttcctatat 171780gagcaaagga ttagctatag gatggtataa aagtcagcac tggataaagc cctaggcaca 171840ttttatgttt tctctacata ccacatgtta aaccttttag cacatcagta tgcatagatg 171900gggcaaagag gaagacatac ttactatatt aggtctacct aattaggact gaattaattc 171960cattattacc ttttttttga tagaaaatca tacctcctgc attatgcaag caatagctga 172020ttatttgtaa tagtgtacaa acaatggcag gtattcactc catcacaggt ttcctacctc 172080accaaatgaa aaggaataat taaataagag agcctagttt tcaaaaggct gcttcattta 172140atattcataa caacttcaaa actgaaaaat aatgtaatgc ctgtcagatg atcttccatc 172200ctttgaaaag gttagaagaa gctgttgtga gcagttcagc ttgtgtatcc cagggaatgg 172260tagctgtatt gtaacaccaa gttgtctgtt atttcaaact ggtaatttgt tccttccttc 172320aatctaaatg tactgtggtt tttgttagag agctaacatg tataatatga tgctttcctg 172380cagtgagtaa attttttttc ttctccttga ctgattctta accgtagaat aattttctag 172440ctagtccagc tctatgatgt catcctgact cctgacatca aggacatgac cccattgagg 172500atgtgaaggg aactgaaaga gggcacccag attgagaagg gacttcgcct ggatgggcag 172560tgtggtatag ggacggttac agactcattt aggtgggcac tgtggtatac ggagggttat 172620agactcactt acgcaggagg agtaggtctg aatcctgact gccaagtttt tgccaagaac 172680aagtccctta aattttgagt tttttaatct taaaaataag agccttgaag ttgatcttta 172740tttccatctc tgactctgca gttgataaaa ttctgtagct atttcaattg catgtggaag 172800gattttgtaa aatttgaagt gagattacgc atcttttttt cttgtaacac tttttaagta 172860ctatattttc aggtggaaca atgggaaaag taaatcatgt caagcaacaa agctaaacct 172920ttcattttac agatgaggaa accagtggca tatcctcttg ggtcctcgtg cccttccact 172980ctgcctcttt tgttaaaccc agtggctttg cataagtcac cagaagtctt ccaatgtcat 173040tttattagac tgatttcact cggatatctt gggacccagt gttttcccat tagtcttcat 173100cagcctaaaa tcatcttaat cctgtccttc ctggagccct ccctgaatca ttggccttgt 173160gtatgactcc tttgcattga ctggattgag aattttccat tctctttttc tcaaatgtct 173220gttgcaattt tagggaaact tttcacactg tccttatttt tacacaaaat ctgcaaaaag 173280aaaaatcttt ttttgcacat tgtattgttg gtgcaaatgt aattgcggtt tcggaccatg 173340gattttaaat gataactagg cctaaataca tctttattaa tcaaaatagg aaacactaca 173400atcaacacat ttttgccaag gagaaataag tgtgtttatt cctgtagcat aaaactccgt 173460gctttgggat tcggcaaact cttgaaaagc actttctgca tcctgctggt tgtggaagaa 173520ttttctctgc aaaaagttgt caagatgctt gaagaaatgg tagttggttg gcgagaggtc 173580atgtgaatat gatggatgag gcaaaacttc gtagcccaat tcattcaact tttgaagcgc 173640cggttgtgtg atgtgcagcc aggcgttgtc acggagaaga actgggccct ttttgttgac 173700cgacgccggc tgcaggtgtt gcagttttcg ctgcatctca tcgatctgct gagcatactt 173760ctcagatgtg atgttttcgc tgggattcag aaagctatag tggatcacac cggcagcagc 173820ccaccaaaca gtgaccataa cctttttttt tttttttttt tttttggggc aagtttggct 173880ttggagcttc tcagtccaac agctgagctg gttgtcagtg gttgtcatat aaaatccaat 173940tttcgtccca cgtcacaatc cgactgagaa atggttcatt gttgttgcac agaataagag 174000aagatgcttc caaacaacaa tttatttttg cttggctcat gaggcaccta cttattgagt 174060tttttcacct ttccgatttg cttcaaatgt agaaagacca tagaatggtc aatgttgaat 174120tcttcagcaa cttctcgtgt agttgtaaga gaatcagctt tgattattgt tcttgagtag 174180tcgctgtcaa cttccaatgg tcagccacta tgctcctcat cttcaagtcc ctcgtctcct 174240ttgcaaagct tcgtgaacca ccaccgcact gtacgttcat tagcagttcc tgggccaaat 174300gcattgttga tattgcaagt tgtctccact gctttacgac ccattttgaa ctcgaataag 174360aaagtcacac aaattcgctt tttgtctgac atcatttcca tagtctaaaa taaacataaa 174420cagcaagtaa taagtcatta gcaaaaacgc ataaagcgag aaatgcgcat taaaatgata 174480tataacataa ccacatttat ttaagaatgt attccaatat caaatggcaa attccaacag 174540tgcaaaaacc gcaattactt ttgcattcac ctataataaa ggttttcaaa gtagttgttt 174600gttatctcaa agctggttta agatattgta atgaagtcaa atgttctgta ctgcactttt 174660atacagttga tacagagaaa atcaacatta tatttgaaac tacagattta aaatgtttct 174720ctgaaaatat tcaagacgtc tagcatgaaa ctgattcatt caagcaaaca gattataagt 174780gtatctttca gtttcattag atttgattac cttgcattct taatgtgcct tacctttaaa 174840tttaattttc tttgcgtact gtgttgagca ctcatgatag ttttttttac tatggttagt 174900tttatttgct ttcattttat tttctttata acctattttt cctctcctgt ccacctgatt 174960tttgaattgt ctgtatatag ctatagacat agatgctact ggcttagatg cagaagaaaa 175020tgatattcca gcaaaccacc gctcccctaa acccagtgca aacagtgtaa cgtcacccca 175080ctccaaagag aaaagaatgc ccttctttaa gaaggtaaca ttaacttcca agctcccatt 175140gtccacctgc tcaactgcac tgcgtcacat ttctagtcct gttgactgtc tgcgtccttt 175200gataagccaa taacgtgcat gctctgttat ttgtttcttt tccatgctgc tgtagctaag 175260cagaagcaga aatcggtaag tttacattga cataagctgt gtttatcctg ccactggcat 175320cattagcatt aattcccaca gttgtattaa atacaatcat ttctgtccca agcaaagaac 175380ctatcaacag ctaattgagt tcatgcattt caaaccaact aaattcaggg agtgacctgt 175440taaaacacca ttacagtact tatccttttt gatggaataa tatctaccac caagggaatt 175500tgtttcaaca ttcagggatg tcaaacactg acagctccac ataatgcacc taaattgtgc 175560atgcttattc tatctctttc ttttatccag aaatttaact tatgatcaaa catattaaag 175620cagttattaa attctgactt atgatatcca gatacatagc ttgtactaaa aaaaaaagta 175680ttcaagtttt aatttagtca gtatttaaac ttctaatcca ctaggtgcaa aatctgcaga 175740tgaacaagac cagtggaaaa ctgcaggctt gttttggcgg tttactgtga gtttttccta 175800ttgtcatata taaatattct cccgatgttc ttgccttctc catcagtcag attttaccat 175860ctcacccttt ggacagtgca gccagtggtc atgcgtagag cctttgccat tggccaacca 175920agatgctaca cagcgaggct ccttgtcccc taaatggaaa ctataggnnn nnnnnnnnnn 175980nnnaacctcc ccccaaattt cccttttttt cccctctttt acccatcagt ttccacagct 176040accatgctgt cttttagaat catactaatg tcgccaagtc tggcaaaacc atggatatat 176100tctcacacgc agatgtgatg tcaaatctgt ctatcctctg cccccaaact ctccacataa 176160tgaacacttt tcctctgcaa acatgtattt tggtttgtgg aggaactcaa gtcattaatg 176220aggtgtcaga tacagaactg taaagttggc tttttttgaa tcaattatga ttattttaat 176280aagggaaaac ttcagcactc tgggcaaaaa ttgcaacact tggatttttg tgttaacata 176340gcctcagctt gatgttcacc cacaacagtg ggcccatttc tggggtccga tcttcttgtt 176400acagctaaag cttgcagcca agtgactaac agtgtccatc attcagaagc ttagaaaatc 176460ggcatcgcta agacatttgg gccttgacgg agcaggaatg tgagctaaaa ccctgtttgt 176520caagataggt ttgtgaagtt tgcttacctt taaattctaa aactttaaaa acttttatca 176580caattttttc actaaataat ttgagcaggc agggcgcaat ggctcacacc tgtaatccca 176640gccctttagg aggctgaggc aggtgcatca cctgtggtca ggagttttga gaccagcctg 176700gccaacatgg tgaaacctca tctctgctaa aaatacaaaa aattagcctg gcatggtggc 176760aggcacctgt aatcccagct acttgggagg ctgtggcaga agaattgctt aaacccggaa 176820ggcggagatt gcagtgagcc gagaccatgc cactgcactc cggcctgggc aacagagcta 176880gataactctg tctcaaaaaa aagaaaaaaa aatcaagcta aatggggtta acagggaaga 176940gtagataaaa atgatggcgt cattcttcat agggcacaaa gcaccaatat tccatcaaat 177000aaggtaattt ctgtagctac cctattgatt aagaagctga ttgattacca gcaacgggtg 177060accctttgct agttggcagt tacagatcga aggaaccaaa gacctatcaa acactgggcc 177120acttcagaat taaagtcttg agataaaatt tcttggtagc tgttactaaa atgcaggaaa 177180tttacattca taatttcaac ggtggaaaag gtgttcttac accgcatagt tatcaggact 177240tggcttctaa acattttcag tatgatccca ggtggtataa atgaaatagt ttaagaaata 177300tgcttatgat gccttcggca ctggtattca ttcttcttat gttattgata atgtggttaa 177360catcaatgta gaattcaaaa acaaaacaac agcctgagat tcaatgagaa gatcgttttc 177420tatgttttag gttgctttta gagtggtgct tgtgtggtac gtgtgctgtc actgtattat 177480gtttgtcttg tttttttttt ttctgttcaa aactgtattc taccttgatt gtgtcaatta 177540aaattaattc tgaatgctag gtgaaatttt aggcagtgaa atttgacagc acagtcatgg 177600taatattttt cttccctctg attatatcgt ctgtgactac gggtaaaatc tcagatggaa 177660gcttttgaat ttgaaatgct ttttaattct cccctgaggc aatgtgaatt caaatgaagc 177720cagaaaagtt tgtaatgggc aattttaagc aacaataaaa tgttcttgag tgacgggcag 177780atgcaaaaat gacattcata tctgcacatc agtgtactga attcttaaaa tcatttacag 177840aacatttttc tgttggtgtg tgtgattctc aagttatatt ttttgacact ccttagccag 177900aaagattcat atagcaatga tttgcttatc gcagtgtcta aggccatatc actcttatgc 177960atttttttaa aaagtcattt ttgggtcaat ttagcatttt atgctatcaa aagagccaat 178020tttaatataa accaatttgc tgttacctaa tttttggaaa gatgttattt taccatttta 178080tttgaagcaa ttaacctgaa aagtgttgat agttttagaa attgctacaa aatttagctc 178140ctcccctctt attaaaaaga aataatttac tgcagaaaga tgtcattagt tatctgacag 178200aaaactactt ttgagaatca tattagatat gcacattgat aatgaacctt ttgagaccac 178260aatagcatgg ttgaatatat tattatacat taatcacctt ccctcgaaga cacataaacc 178320ttacctttga agtaccaagg tatagaaacc aaaaaattca atagcattaa tcactaagct 178380gtgagcaggg atcatgatta ctgtttacta aaataagcat gcatgttcat ttaaactctc 178440acaataatat agaattttga cagtagtgag agttacaata ttttgagatt aggtaaaaag 178500tattggtagt gagagttata atattttgaa atgaggtttt acacaggtaa gacaaaaacc 178560taagatgcaa aggtaatgca atctttcttt cctctgtaat taaccatgtt aggtctaatc 178620actacaactc tttagaaaat tcaaatgcag ccgaaaagca atattaactt ctagtcaagc 178680ctaaagcacc tatcttgcat gaggcatgtt tctctttgtt ctgtcaacta gtttttgttc 178740aaaagcagca tctcttgctt taggaacgaa taggtctgta gcatttcttg agatgagcaa 178800gaaaactgta gacaacagta acttgaaact tcaggaaaat gaacccacca atctttgact 178860gggtttatgt agaaaagagg caagtgggga aaataaaatg tctggaaagc cagtgcctca 178920tattatggag ttcagaaaga gtaccttata cacaaaatat ttatgttcta cctgcctgtg 178980aaacgtctaa aagcctctcc tctctctgca gacagagcac actcctccgt atgatgtggt 179040accttccatg cgaccagtgg tcctagtggg cccttctctg aagggctacg aggtgggtag 179100cagccttcca caggaagctt aacttgcatg ctgaacttct ttgtgatgct gcctcctact 179160cccgaccctc tctctgaatt ttacagctta aggagccaac tttagagctt agagagctca 179220cagcagcaaa gcctgttgtt ctgcaaactg gtatacactg cttcgtcctt tttctgtttt 179280gtttacctgg ctgttgcttt gaagatttct gctagctgca gtgtactcag tccaggaaat 179340agtgtcaaat ggagaccgac tgaaaccact tgcaatgtta tttgtcacta aaacatcatg 179400ataagttcag tttaattgtc attgcatcag aactcagaag cagaaaaatg cggcaacctc 179460atattgccac tctttccaga tgcaaagctc aggttttcaa gcattcctaa gagaagtaaa 179520attgttttat catcgttagt aatttttttt ggtcatatct taatttattg cttgctcaat 179580tgcaggtcac agatatgatg caaaaagcgc tgtttgattt tttaaaacac agatttgaag 179640ggcggtgagt atttcagcat actgggtttt gtggattttg tcttaaagat ggcaaaacgc 179700tggtgggaca tgcgtgtgat tccaagagaa aatggccctc tgatgtctat atgatgacag 179760gaaacaagtt tcactcaatt taatctgatt caatcaatat ttatcatatt tgcttggtac 179820atctttaacc cctgatgtct agttcctgta cctacatcca taagctgacc ttgggcagga 179880caccactggc cagtctcacc ctcaattcat ggtcaactgg ccctctattg ctgcccaggc 179940atcgcactaa atatccctct tccattcact tttccactct tctaaaagac ttctgtgcca 180000tctccccctc cttaatcctc tgataacccc ttctcatctc acgctccccc agtgaacttg 180060cttccgtgtc attgagaaaa tagaggcaaa cagagaacat ccaggcattt ctacaacccc 180120gcctgtggag ttgcatgttt gcctgcatgc tctgctttat ctccaatgac tatgttccca 180180gcaagggcca acccaccatg tgggcactag atctcattcc ccatcccctc tctgctagaa 180240gtatccatac agagccgtaa tcatcctttg ctctctcctc atgagtattt cctgttctaa 180300tggaaactcc atcaccatgt aaaaatgtta attcctatca cattgacctc atattgctct 180360aggtgtctca tttttttttc ttttctttac aggaagattc cttgaaagaa ttgagtgtac 180420tggctttcct tccactttct tcagcctacc ccagttatgt tttcacccac accactcagc 180480taaactagtc atgtcaagat cacccatgac cttcacattg ctaaatccag ggataaattt 180540tcagccctaa tcttacttga ccatgagcag tagttgacat ggtggattat tccatcctct 180600ttgaaatact ttgcttggtt tctggaacca cacttttctg gttttccttc tatcacactg 180660actgctcctt tgtctccttt gctgatactt cctcatcaac tggaaatctt ggacagccca 180720gggctaagct ctatgatgtc ttttacctac atttctttag tgatttcatt cagcatcaga 180780tttttaaatt ccattccttt gccagtcttt cctctaaatt ctagacccat gtatacaatt 180840gcctattcat tatcactact aagatgtcta atgggacttt tcaaatgtaa cctcccccaa 180900aacttactcc tccccatggg ccttccctat ctcaattaat agcttcttcg tctttcagtc 180960ccgagaccct tcagcccaga tcattttcct tgactgttct ttctctctta tatttcacat 181020gcaaatgctg ttgtctttat cttcaaaata tattcagaat cgaagccctt ctcacacatc 181080ctctgccacc attttggtct gagacatctt catctcttcc ctggattggt gcaacagcct 181140cccaccttgt ctccttgctt ctgtggttgt tcccttaaag cctgtgctca ggttagctgc 181200tgcatgaccc tgttaagatg tgagctagtg cccaccactc ctttactcaa aaccatccag 181260tgacttcttt ttttcctctg aatggaaacc aaagtcctac agtggcctac atgacctcct 181320atggtctatt cttccacacg tccttaccta gattttctct tgctgctctt ccctcgctca 181380ttctcaccct gccgtactta ctcccttgct gttctttgag tgttccagac agattacaca 181440atcacctcag ggctttggca ctttgcagtt ccttctgcta ggaacacttt tccctcagat 181500attctccttg gtatacgtgt gtacatgtat gcacacatac acatgcacac atttgttctg 181560tgtctctcct ttccctctgt tttctagtgc tcttttatgt atttccagta ctatttttgt 181620ttattgtcat tctcccataa ttagacgata aactccatga gggcagggtt tgagaatgtt 181680ttgtgaactg ttgtattctc agtgcctgga gcagtactca acatgagcag tggccagatg 181740aattttgaat gaacgtctgc tgcatactct ctgctagaga aatgcggaga tggtgaagcc 181800agtctctgcc tatgaacagc tctcatggaa accataaatt gaaacctgta gcagtaacac 181860tgcatataag taaaagggca acttggttat atgcatgcac aaggtgcagg tagcataaag 181920ggaggcgtaa ttaacaccaa cttcaggaca tccacacctg gaagaatttt taaatggtgc 181980ctaacgtttg accaaatgga ttacagggaa gagaagggta tgttcagcag atggtatata 182040gtgtgtgaag gctttgaggt atgacacagc attatgtgta aggcaactca cacccacatc 182100ttaaaggaca tgggcaagga gtgactggta acaaatccaa gggtcatatc tagtgtgtca 182160cgccaatggg cttggactta atcctttctt tctcctcctc ttcccccggt cactattcaa 182220cttgcctcag taagtattta ttgagtatat tctatgtgcc aggcattgtg aggagcaaaa 182280atagacaagg gccttggctt tgtggggctt aaagtctatt gaaaggcagt cttgaatcaa 182340ataattaatg ggtgccatca ttgaatgtta agccagctcc tgaagcatca aagacatgac 182400tagagtccaa aatggaaacc tcaaagttta agagtaggtt attacagggg acctggaaga 182460tacagggcta gtgtatattt ctgtattttt aaccagataa atgcagtgct tctcttctcc 182520acattccttt cctcctccca tcctaacaat gcaactacaa tagatcagga gtcccaaatc 182580cccgggtcac agatcagtac tggtccatgg cctgctaggg gccacacagc agacggtgag 182640tggcagggga gtgtgcaaag cttcatctgt atttacagtc actcctcatc actcacacat 182700tattgcctga gctcagcctc ttgtcagatc agcagcggca ttagattctc ataggagcac 182760aaactctact gtgaactctg catgcgaggg atctaggtta catgctcctt aggacagtct 182820aatgcctggt gttctttcac tgtctcccat caaccccata aaggaccatc tagttgcagg 182880aaaacaagct cagggatccc actgattcta cattatggtg agttgtataa tcacttcatt 182940atatattcca gtgtaataat aatagaaaga aagtacacaa taaatgtaat gtgcctgaat 183000catctcaaaa ccatctcccc actcctacca aaccggtcca cggaaaagtt gtctttaatg 183060aaactggtcc ctggtgccaa aaaggttggg gactgctgca atagatagta tttgttggtt 183120tcaggattct tttagatttc ttactatagt actttctttc tagaccacac ctactactac 183180aaccatttct aatttatatt gacttttggc aggggaggca agggtaggaa ggaaagtttt 183240ataggaggac aagtcttttt agtaagtgat caagcaggct gggcttggtg gctcctgcct 183300gtaatcccag cactttggga ggccgaggcg ggtggataat gaggtcaagt gttcgagacc 183360ggcttcgcca acatggtgaa acctcgtctc tactaaagat aagaaaatta gctgggcatg 183420ttggcgggtg cctgcagtcc cagctactcg aaaagctgag gcaggagaat tgcttgaacc 183480cgggaggcag acgttgcagt gagccgagat cgtgccactg ccctccagcc tgggaaacag 183540agtgagactc tgtctcaaaa aaaaaaaaaa aagtgatcag gcaaagaact gaaaggagag 183600agaatgagga aaacaaaaac tattataagt ttcaaggtat gaaaagactt tataatcaag 183660gtcttctaac caaatattcc taattagatt tttttaattt tccatatctt taagaaatat 183720ttttcagttt gtgacaggaa atagaccctt tagcttggac caaggctact aggcactggc 183780caagtataaa aggcatagct ctactctgct ccatttagct tgtttcttta tccttggctc 183840tgtcatttat cacaatttgg aattgtcaac atgagcctga acactaaaaa aattctgcta 183900acaaaggcac tgtgttttcc atgcctgagt caacccactg ccctttccta aatgccaaag 183960atggggaaat gggatggaat ttcctggatt actacttgta cttaaacctg agtaagtttt 184020gaatttggaa gacacattcc agccagagag ggtggatttt ggacccatgt tcgagtgtat 184080cagccaccca gattggtttt atttaagaaa cagataaaca aaatatctta aagtatctgt 184140tatttctagc cagaagtaac ttccgtatgg tttaattaat agacctgcct tggctcatct 184200tgacttacag agctggggtt ctatattcta gccctgttct ccccagttaa cagtcctcag 184260aggaagaaac gagtccataa ggcagaattt aaaagctgtg tacaaattta acatttttac 184320ccatggcaag acatctagag taactgaatt attgactgtc tttgcttttg gcaaggcaag 184380acagcaatag aggatggaaa ttcaacaaaa gtatcaggtg tgtatataca tatgacattc 184440taaaagggaa aaatgaatta ttccttaatc atctattaaa taagctaagt ttatttttta 184500aaaacgttta agcccaagag gagaaaatat gaaaggaaat gttagttatg cgaaaggttc 184560taggtagtca agaattcctt tttcttttct gaaacccact taaattcatt tgcagcagcc 184620ctagggggtt gtcagaatca cattctggtt aagtagtgtc agtgtgtcag atcaaagaag 184680aaataaatca tcctcctcct cctcctgttg tgtgcaataa agtagcccat tcaaaactgg 184740attcacagta accaactgtg tcatgtaaac aaagcaatct tcagattcac cttgtaggat 184800ggtgacaggt aaattaacaa ggaagttgag aacaagaagg aaagctacct gggataaatt 184860cacggttttt tttttttgtt tttttgtttt gttttgtttc cttcagtgac tttcaaaacg 184920tcagcaatca gccctgaagt gtttggaatg catactgaat tgctacaggg ataaatatac 184980cattgccaga atcttctgta ggacaatctt actagaaatc agtaatcctg aggctgagca 185040cagtggctgt cacctgtaat cccagcactt tgggaggctg aggcaggcag atcacttgag 185100gccaggagtt tgagaccagc ctggccaaca tggtgatacc tgtttctact aaaagtacaa 185160aaattagctg ggcacaatgg tgggcacctg taatcccagc tactcaggag gctgaggcag 185220gagaagcgcc tgaacccagg aggcggagat tgcagtaagc caagatcaca ccattgcatt 185280ctagcctggg tgacagagca agactctatc ttaaaaaaaa aaaaaaaaaa aattgagcaa 185340ataaaaagtt gtttatctca tggaaaccac gaggaaaata cacaagaaaa agtatagtag 185400gctgggtgca gcagttcaca cctgtaatcc cagcacttta ggaggctaag ggaggaggac 185460tgcttgagct cagaagtttc agaccagcct gggcaacata atgaggccct gtttctacta 185520aaaaaaaaaa aaaaaaatta gctaggtgtg gtggcatgca cctgtagtcc cagctacttg 185580ggaggctgag ccagggggat cacttgagcc tgggaggttg aggctgcagt gagctatgat 185640tactacaccc ttgcactcca gcctgggtga cagtgagatg ctgtctaaaa aaaaaaaaaa 185700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 185760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 185820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 185880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 185940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 186420nnnnnnnnnn nnnnnncttt tgcttttttt ctatttctat tatcatgtta cttccaaaag 186480ctcttattct aagtctttaa atagaaattt gttctagtct catagataaa accatctttt 186540agcttttaac tgtgaataat tgtcataatt gatttttctg tttgttttgc tctctcatga 186600ggttttctga agatgcctga caaactgtcc tgtgctcaaa ttttaaaata aggcataaaa 186660taattaatca gaggttctgt gctcataggc ggagcttgtc aactttgtgt ttctgtgtat 186720ggcaatcttg ctagataatt tacacaggaa acctccaata tcattatctt tgggtcctta 186780caatttggca gtcagattcc ccagagaaga atcttccatt ctgacccctg gggatataaa 186840tataagcctg gctaccaaaa ctgtacaatg agaagaaagg ctgaagactc aacattcaga 186900atcctttctt ttccatccag tattccctgc tcctgttctg tctagggtcc cctggtcaga 186960ggccctcttg tattgcctgc aagataacaa atgtagggtc ttgtaccaca caggaaaaga 187020actgtcacct ggctatttga tattgtgagg gggcagtgga gggctgtaac tgaaacagac 187080tttttaacca aacttttcct tttcgcccta acttcattct ctaccttctt tagaaatatt 187140gtgagccttc aattttgctt cacagctttt tcctactgct gacttgaata ttcagctttc 187200tgcagtctaa gtcatttgct atttccagtt cccaaatttt gttgctctca tttctacctt 187260atctaaaatg atactcccaa ttcaatattg tctctcataa taccctgtat gttctttcca 187320caacaatcat catctttaat tattttcctt tattatttat ctctgccact agaccattag 187380ctatctgagg gaagactggt tcttatttat ttttcatgcc tatcacagca atgaactatg 187440gcacagagac taggaatata gggtcaggag tctgggttca aatcccagct ctcccactta 187500gttacataca tacccttgga taaaaaatct ctctgtgagt ctcatttttc atcagcaaca 187560gtgggataat agtaaacgtt tctcctagag ctttttggat catttagtaa ttcaaataaa 187620gtgctcagta cttatctgac acttgtaagc aacaagtaag tgcaatgtaa ggcaggtgca 187680ggatagggcc gggcgcggtg gctcacacct ataatcccag cactttggaa ggccgaggga 187740ggcggatcac ttgaggccag gaattcaaga ccagcttcca tagtgaaacc ccctctgtac 187800tgaaaataca aaaattagcc gggtgtggtg gcacacacct gtagtcccag ctactccaga 187860ggctgaggca ggagaatcac ttgaacctga aaggcggaga ttgcagtgag ccgagaacgc 187920accactgcat tgcagcctgg gcagcctggg cgagactctg tctcaaaaca aacaaaaaac 187980aaagtaaggc aggtgcaaga taaaaatgaa taagtaaaaa gaaaaaaaat gaataagtaa 188040gtatcctaac acatatggtt tgaaaatatg gttgctatat atatttcata cttagatgaa 188100tttggggcat acacttctat cccctttgat tagaccaata accttaaggt cttctgtgac 188160tttttcctcc aacaggatat ccatcacaag ggtcaccgct gacatctcgc ttgccaaacg 188220ctcggtatta aacaatccca gtaagcacgc aataatagaa agatccaaca caaggtcaag 188280cttaggtaag tttgtgcaat gagcttaagc tttttaaact ctcctctccc gatgttgatg 188340atgagaagac ctaacaagat gataattcca tgtgtcacag agtatagaaa aaacaggtgt 188400gtgctgccag tcgctgttgc tatggtctta ctgatttcat ggtgtggaag gatttgttat 188460gatagtttca agttaagaat tccacaggct gtcagcatta atgttttggt tgtggtttgt 188520tttcactaac atgagctttg gtgtctgcag actctaagat ctcctggctt tgcatcagtg 188580ttaccatgag caagtttatt aaccttttga agcctccatt tcctcatcct aaaaaagaaa 188640taaaataact acctcataga atcattgcaa ggattaaaca tgatatgtgc tcaataaatg 188700atatccatta ttatcctaaa tattccttaa ttccagttat atatcaataa tgaaatcatt 188760ctcaggagag tataatttgt gggtgtggaa attagtaaaa gatgaagtca attcctggct 188820atatttattg atagatatca atatgtttgt tttatttgta aaaacagtgt agggagttat 188880tgtgtttaca gagatggtat gtggatactg cttattaacg tatactgaat tgatcacact 188940gagacccttc agctgctatt taatcttttt ggtatgtgct tggagtttta ggttaatcac 189000tgttacacat gttgcttact ggatggttag gtctctgttt ttataaagta aactttctga 189060gagttgtgtc agatctttga gcttaatttt tctctgttta taggttagat atacaaatcc 189120tctgagaggc tggtttctaa catagaagtt cttcgtttac tctgaagtat gattatataa 189180ataacagaaa aactattata aataataagc tttatatgtt agcaatgagc atgatacatt 189240atagaactca gttttgtttc tcacctaaca acccttcgtt ggcaaaactt cataacaggt 189300ttagaaatat atttgaggaa aaaatgtaat catgtgtttt gcttatagca attccttttt 189360atgtccttat catctgtcac agttaaaact ctttccacac atcatttgat gctcacaaga 189420attacttttt attttgactt ttttgagaga gggtcgcact ctgttaccca ggctggagta 189480cagtggtgtg gatcatggct cactgcacct tgacctcatg ggctcaatca aacctcccac 189540ctcagcctcc tgagtatctg ggactacagg cacatgccac tacattgggc taattttttg 189600tagagaggga gtctcactat gttgcccacg ctggtctcca attcccaggc ttgggcaatc 189660ctgccttggc ctcctgaagt gccaggacta taggtatgag ccactgtgcc tggccaagaa 189720ttattataaa atgcttattt cataaattaa actgaattaa caaagtcaag taatttttta 189780gactcatcag tggaatcaat tacataacag atttccttag attgccaaga ttttttttac 189840aactactgat tatacattgt ttttaaatga attttccagt ctatgtattt cattgagcat 189900acactagata ccaaatatca tgtggaagcc catgaataaa aaaagaaact gatgaagccc 189960cttccctcaa ggagctgggt gtagccataa ggcgaatcag tgaagagccc tttgagatag 190020agggtgatga gcaaagtggt cgagccatga actggctgcc aaagagctgt tgctgcagga 190080ggcacaggga aaggcttttc cagggagctg atcctgagct gagacttgaa gatgacagga 190140tttagccaag aaaaaaggga aaggagtgga gagggtgaca gtgattcgag acagtgaaag 190200ccacaacagg cttctgaaaa acataggtgc ttcatttaag ctaaaatata ttgttttaaa 190260aagaaggaca tgggagatga catgacagag agaggcaggc ataagacatg aaaaggttgg 190320agcttatttc tattagtctg ttctcaggaa aaaatgtgat catgtgtttc gcttatagca 190380atttcttttt tatgtcctta tctatcacac ttaaaactct ttccacacat catttgacgc 190440tcacaagaat tactgctaat aaagacatac ccaagactgg gtaaagtaaa gaggtttaat 190500ggaatcacag ttccacatgg ctagtgaggc ctcacaatca tggtagacag tgaaggagga 190560gcaaagtcac atcttacatg gcagcaggca agagagagca tgtgcagggg aactcccctt 190620tatgaaacca ttagatctca tgagactcat tcactatcag gagaactaca tgggaaagac 190680caagtcccat gattcaatta ccccccacca ggtccctcct gtgacacatg ggaattatgg 190740gagctacaat tcaagatgag atttcggtgg gcacagccaa accatgtcat tattcttaag 190800gtggtaggag accatgtaca gactggaggc attcattcat ttagcaaata cttgaatacc 190860aaccatgtgc cagccattat tttggtgccc agggacacaa aagtgaagga cacaaagtcc 190920tctttctcat agtgtttgtt ttacaggaag acatggacaa aatcttgtat atatagacag 190980acagagatgg ctggtcagtg gttgagaatt ctaaaagcag cagcagacca aggaataaag 191040agtgagccaa ggaggaggct gtaatattat tataataaaa tgcaagaagt acaaaaatgc 191100aaaaaaaaaa aaaaaatgca gttgggcaaa ccaactgcat ctaacaccaa acccatttta 191160taataaagtg tagagtatat catgtaatgt attgaatact aaaagtgaaa aacagaatgg 191220ttgtatgggg actcaaagtt cggttcccac tgtacatata ttgctttcac actcttataa 191280agttgaaaaa tctttaagtc aaaccatcgt aagtcatgga ctgtctgcac ttagtgtagg 191340ccaagtactg cagtaactat aacggaagct ttgcatgtgg attaatttag tcctcatttc 191400cgtaatacag attagaatac ttaggttcta agaggttcaa tatgttaccc actggccata 191460aagctctatg aagcgatgac gcttcacttc tgccccccaa gccactttga tatgtggcag 191520tgggaacaga gaggcagaat ttactgagcc tggggagaaa ggtagttaga gaaggcttat 191580acagattctt tttgcaagat aaggcaggca ctggtcaaaa ttaagagttt tgtgtgtagt 191640aattatgaaa atggcattgg aattacacat atggcattaa ataactcata ctagagctac 191700ctccaggtct tattaattcc taactgacca cttaggtgtc cctcaacact agccgcaatg 191760aaagtaacca tctctactct gttattacag tattttttgt ttgattacag aaacctcctt 191820ctaaataagt taacttctgt tttttgtttt ttaactttta agttcctatc acccaggtat 191880taagactagt actcaccagt tattttttcc tgattctctt cttcctccca ccctgatagg 191940ccccagtgtg tgtgtcatta accctctatg taccttgttt tctttatcca ctccatcatt 192000gatgggcctt taggttgatt ccacatcttt gctattgtga atagtgctgc agtgaacata 192060tacatgcgtg tatctttata atagaatgat ttatatttct ttgggtatat acccagtagt 192120gggattgctg ggtcaaatgg tattcctatc tttatgtctt tgaggaatta ccatactgtc 192180ttccacaatg gtttaactaa tttacactcc catcaacagt gtataagcgt tcctttttct 192240ccataacctc aacagcatct gttatttttt gactttttaa taatagccat tctaactggt 192300gggagatggt atctcattgt ggtttgattt gcatttctct aatgatcagt gttgtagctt 192360ctcttcttta tctaagagga aacattgttc ctgttgttgt ttatagatat tagagatata 192420aagcatagaa aggtgttctt catttttcag tttaattcag tgggtataag aaactatggg 192480atgtggggag atgaggaagg cttgccccgt cttcaagatg ctgagctgca ttctttcatt 192540agctaggaca actggaaaga atcaaattga ctttcagttt cagaaacgaa cattggggaa 192600gtttctgaca tttctttatt agagtaaatt ctgagcatga tatactctgt caaaggaaaa 192660ttcctagcat ggatacatcc atttttaaaa gcatcatttt tactttgaag tcaaaagtat 192720tatcagaatt tgtcgtcgat tcttaaggtt gtatctacta atacagtgtt gcaccaggat 192780tctgaaagtc cactttaaaa tattttaaaa ctctttccct ttaattaacg gagtgtaaag 192840tatgtatgta aggctaccac aaatatcttt atatatgtgt atatattaaa acatgttaat 192900atattgacat aatataaata atatccatat atgtaatata ttttaaggct atccaggttt 192960tatacaatca gcacatatta gggctgggcg cagtggctca cgcctgtaat gccagcactt 193020tgggaggcta aggcgggaga tcacttgagg tcaggagttt gagaccagcc ttgccaacat 193080ggtgaaacgc catctctact aaaagtacaa aacattagct gggcctggtg gcacatgccg 193140gtaattccag ctatgtggga ggctgaggcg agagaatcac ttgaactgag gaggtggagg 193200agattgtggt gagccgagac tgcaccactg cactccagcc tgggagacag agagagactc 193260tgtctcaaaa aaaaaaaaaa aaaaaaaaaa accaaaaaaa caaacaaaca aaaaaaacaa 193320aaaaacccac atattaaact tgtactactc aaacctttaa aagcaaggag ttacacggga 193380tgctgtgact gtctgaacac ttaacagatg aaacaaagat gaaaatcttt gtgatcagat 193440ttcttaatgc ttacagtaaa tattaattta ggttgtaact tgactgggat tcagtggccc 193500aatgttattt taaagctagt atctcatgta gtttgggccc tgatttttta aagatatcta 193560ggtaaaaaaa aaaggactca agtcataaca tattgtgagg cagatggatt agtgaccaga 193620gagttacctt ttcttcaata aggtactagt taaaattatc tccaggaagc ttctggcaag 193680aatccaacgg cctaagagga attaaaaggt aattgtaaat ccacatttgc tagcataaaa 193740gtctcatgga attcaacctc cacgactgca gtgggaagtg aaagctatgt actatgcttt 193800atagacctat gctgcctagt aatagtagct tctgcctatt tattaaattt taaaaattta 193860cacgcctgtt caagtgctca gtagcccatt agctgctgcc acgctggacc acatacagag 193920aatatttccc tcattgcaga aaattcggtc agacagtccg gtttttgagt ctttggagat 193980cacagaacag atgaactgaa tgcaattttc tcggagttca actagatatt ctaatgtttt 194040gtgataagtt tctataaatt ttatacttta gatttctgta gtgctgcctc tgaaaactgc 194100ccttttaaaa catgactact ttggttggaa aatatgaggt gtgtttttgg ataagtttct 194160ttctggtgcc attcacgtct ttgctgtttt attttgtatt tcatcttgtt aataaacatc 194220tagacggcat acaggtgaca tgtaaacata gatcagcccg tgcactgaaa ggaaaacctg 194280tcatggttgc taatgcaaag tggtctgcaa tgaacacacg gaagatatgt caaggttctt 194340attttacagc agttttatga aatgatttca tagactgcaa gcacaaagaa tgtcagcaga 194400gagctaaaaa tttccacaat ccaagtatta tactaaaagt aaattctgta gccggttgac 194460agaaaaattc cttaaatcct aatgatgtgt ggagaaacag ccctttccaa aaggataaat 194520tactgttgta aatttttatc aattctattg cctgtaagca ttaacataat ggctgaccta 194580ctcacctttc tgttgaagaa acagtatacc attttacttt atcttaaaaa tactgcactt 194640taactgaatt gtttcgccct ttacagcgga agttcagagt gaaatcgaaa ggatttttga 194700acttgcaaga acattgcagt tggtggtcct tgacgcggat acaattaatc atccagctca 194760actcagtaaa acctccttgg cccctattat agtatatgta aagatttctt ctcctaaggt 194820aagtaggact gctactgttt gctctataat caaactttcc taaaatgtat tttatgttct 194880gctttctata attaggctat tgtaatagcc tttatgatgt atagagaatt tgaggagaca 194940tgatagtcaa gaatttttaa attgatatag tttcatggct tagaacagct gttctcaaag 195000tgtggtccat ggatccctgg agagtgttga gacactttta gagggtccat ttagtcaaaa 195060ctacttccat gataatacta agacattatt tgccattttc actgttttga tatttgcatg 195120gctgatgcaa aagcaatggt gggtaaaact gctggcgtct aaacataaat caagactgtg 195180gtatcaaagt accagcagtt actgtattca ccgccaccat gctttcgcag tttgagggga 195240aaaaaagcca atttcactta atgtccttat aaagtagtaa agtttattac ttttattaaa 195300tcttgacttt taatattcag tgtactaaaa caaagtacac acaaagctcc tgctgtatac 195360caaaaagtaa ggacaagcac tggtttgatt gagttgtgaa ctaaactagc catgtttttc 195420atagaatacc atttttatct caaacaacaa ctgacaggct atgcttattc cgacttggtg 195480tagcagatat tttcttggac aaaataagcc tgtcacttaa aggagaacag catgtgttgc 195540caatgataaa actcaagctt tcagacaaca atcagaattt tagaaaactt gtatgcatct 195600ctgagaactt gcaagcttcc caataaagtt ttctctgaga tcaatggtaa tattaatgaa 195660tatgatgttt taataatgta taatgaaata tgtcaacatt tggaagatct gaagtcagtg 195720aaccattatt gtttgtatga tcaatgaata acattaattt tcaagacaga ccaatggaat 195780gtcatggagt aagaaaattg cactgcaggt tcagattcca cataacaaat agtctttaag 195840gaaccaccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 195900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 195960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatttgtt gagttttagt 196020gtagtatcaa agaagactat actaaattgt ttctcctttt ctcaattaca aatctgtagn 196080aggttggatt ttcttcacgt atttcaacct aaacatgaca ttgcagcagg ttgactgtag 196140aagcaaacag gagactgcaa ttgtcttcta tgaagccagc cagccagcca ttagagagat 196200gtgtaaaaat gtagctattt ttattaaaaa aaattcggcc gggcgcagtg gctcatacct 196260gtaatcccag cactctacta ggctgaggtg ggaggatcat gaggtcagga gatcgagacc 196320atcctggcta acacagtgaa accccgtctc tactaaaaat acaaaaaaat tagccgggcg 196380tggtggcggg tgcctgtagt cctagctact tgggaggctg aggcaggaga atggtgtgaa 196440cctgggaggc ggagcttgca gtgagccgag atcgcaccac tgcactccag cctgggcaac 196500agagcgagac tacatctcaa aaatatatat atatatattt taacatgtat cagatttatt 196560gtgaattttt aaataaatgt tttaatagtt ttccatttta gcttctttaa agaggttgtg 196620agataaaatg tttgagagct gctagcatag aaaaaatgtg agtgcttaaa gcttgcaact 196680gtgatttatg ttaacatgtt aattttgcag ataatcttat agctccatct attataagcc 196740ccttgtgctg tatataaaaa ggccccagag aaaggagtca ataatgtacc ttgtacttta 196800cctggatgta gttattactc tgttaaaaac tcattatctt ttacaggttt tacaaaggtt 196860aataaaatct cgagggaaat ctcaagctaa acacctcaac gtccagatgg tagcagctga 196920taaactggct cagtgtcctc cagtaagtta tctctatata cagcataatc cagttacaga 196980gatcagacct tttctttttt tttttttttt tttttttttt tttttttggg gacaaggtct 197040tgctctgttg cccatgttgg gagtgcagtg gtatggtctt agctcactgc agccctgaac 197100tcccgggctc aagtgatcct attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197400nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197460nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197520nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197580nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197640nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197700nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 197940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198180nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198240nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198300nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198360nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198420nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198600nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198660nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198720nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198780nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 198960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 199860nnnnnnnnnn nnnnnncccc acaccacaac catcgcagtg ggacaaagtc gcggcctctc 199920caggcaagag acatttgact cggaaaccca ggagagtcga gactctgcct atgtagagcc 199980aaaggaagat tattcccatg accacgtgga ccactatgcc tcacaccgtg accacaacca 200040cagagacgag acccacggga gcagtgacca cagacacagg gagtcccggc accgttcccg 200100ggacgtggat cgagagcagg accacaacga gtgcaacaag cagcgcagcc gtcataaatc 200160caaggatcgc tactgtgaaa aggatggaga agtgatatca aaaaaacgga atgaggctgg 200220ggagtggaac agggatgttt acatccgcca atgagttttg cccttttgtg tttttttttt 200280tttttttgaa gtcttgtata actaacagca tccccaaaac aaagtctttg gggtctacac 200340tgcaatcata tgtgatctgt cttgtaatat tttgtattat tgctgttgct tgaatagcaa 200400tagcatggat agagtattga gatacttttt cttttgtaag tgctacataa attggcctgg 200460tatggctgca gtcctccggt tgcatactgg actcttcaaa aactgttttg ggtagctgcc 200520acttgaacaa aatctgttgc cacccaggtg atgttagtgt tttaagaaat gtagttgatg 200580tatccaacaa gccagaatca gcacagataa aaagtggaat ttcttgtttc tccagatttt 200640taatacgtta atacgcaggc atctgatttg catattcatt catggaccac tgtttcttgc 200700ttgtacctct ggctgactaa atttggggac agattcagtc ttgccttaca caaaggggat 200760cataaagtta gaatctattt tctatgtact agtactgtgt actgtataga cagtttgtaa 200820atgttatttc tgcaaacaaa caccttctta ttatatataa tatatatata tatcagtttg 200880atcacactat tttagagtct taatgccaag tcagcagatt tgctttatga attacaggga 200940ctagaaatgc ccacattcag gaaatttgta ataacattgt ctagacacct atcctcattc 201000tagtagaaag tgtgtacata ctgtaaatat gtgtgattgc ttgacttgaa aaggtttgaa 201060ttctgaatgt tataccatcc ttgtaagtaa gtttgtaatt tccaccataa attatggtaa 201120atataaaact ccagaggttg ttctactcca tacagttcac actgattgtg acacattctt 201180agtagctagt gtctgttcta gtcactgcac tggagtctac gagccggaac tcgctatatg 201240cacgtgtgtg tgtccgtatg taagaaagtg tgcaccgagt gactgaatgg ttgagatgaa 201300ttggaatgct gaagactaac gaagaaacta gagactgata tcgagcattc tgcccacctc 201360gctctgtatt taattaattg tgctatatgt tgctttaaca acccattgag cagtcaggga 201420atgtgagtaa gcttgctgcc aaaggtaact aggaaagcat tcatctgctg cctccttgtt 201480tttgctccta gagagtgaaa atacaggcaa ttttactgtg agtgtttcac tggaaatgta 201540caatctttgt gtgttagagt atttgtttta gtaagaaatg tttgtttaca cagcttgtgg 201600aattatttcg tgggaaaata aatttttata acttctccca cttcaatttc taaccttgcc 201660tattgttcct gttgtttgtt tacctcccag ttacctacca ttcctcccca ccaccgactc 201720cagcaggttc actgtctgtc agaacccaga agtgcttctt ataaccaaag tttctgttct 201780tcagaagaaa tcagggcaaa atggggtgac ttgaagtgaa taaaatgtta agaatattat 201840cctacataag acatgtacac agaaggggaa ccttgaagac attatctcca tgcctcaatt 201900acactgctgt aagaagctta caatgtcatc atgtttaagg ctaagacctt ttccgtgtct 201960caagtgtata ttttgtccag ttataactgg atggtaagac agtattaaga gtgcaagtac 202020ctggcacttg aagtttgtcc caggaaaatg cctgtgtata attacctaac ttcagatctg 202080cacattaact tatttaacaa aataaatcag ccgggcgcag tggctcatgc ctgtaatccc 202140agcaatttgg gaggctgagg agggtggatc acctgaggtc aggagttcaa gaccagcctg 202200gccaacgtgg cgaaaccccg tctctactaa aaagataaaa aattagccag gtgtggtggt 202260gcacacctgc aatcccagct acttgggagg ctgaggcagg agaattgctt gaacccagga 202320ggtggagcct gcagtaagcc aagatcacac cactgtactc cagcctgggt gacagagtga 202380gactccatct ccaaaaaaga aaaaaaaaca aaaaacaaaa aaaactatcc agcaaaatta 202440taaaatctac tttgttttgc ttccttgatt attccaagtt cttaccaaat attcttagcc 202500ttttataagt aaaactgttt tacatttaac tccttatttc ccttaccccc aagaaaacgc 202560aatattaaaa atgattaagc tggggtggta gagtatacct gtagacccag ctacttggga 202620ggctgagata ggaggatccc ttgagcccag gaggtggagg ctgcagtgaa ccgtgactgt 202680accaatgcac tccagcctgg tgacaacctg gtgtttaaaa aaagaaaata aaaattagtt 202740acatatataa tgttttatca gcttatatat gaaaaaattt ccctcagttc gttaattagc 202800cctacactgt ttacataaat ttatttactg agtataaaat cagtagtcat aataaacttg 202860acaattctta cattggtagg aaaccatatt ctaattaaaa ttgattttat aaaatatttc 202920tgataaaact tttgtcatgg taagagattc acaattatta gttcaatcct ttattatttc 202980atcccaatta atgattttag aatatttaaa ttcttgaaac atagctagta tttatcttac 203040ttgctgctat cattaatccc tttcaaaaag tagtggtgag agtttccatc ttttttattt 203100tgtatctaga ttcttagctt gcactttact gtagaacata ttagtgcaaa tcagaatatt 203160cctcaaagaa ctagcttgaa ggatttgaca taagagccgc tatatgtgaa aaactgtata 203220gtggacatat gtgtaaactg tgtagtaaat ctgtaaatga ggaaataatc tcaagggcaa 203280tgggatattt actgacccgc ggaatgtaaa gttacagtct tttcacacaa caaaggctta 203340accttaacct actcagttgt agagccattt ttgtagtcaa cctagaaaat gctggaaatg 203400tatttaatag tttttttttt ttttttttgg tcatatccat ttcagtcttt cctatgctct 203460ttctctactg ctcatttaag ttactgttac aaaaaggtgc tgctaaatgt aggatgtctt 203520agtcatgttg tactttggga caatgccaca tttttaacat ggtctgctat gcattcctgt 203580taaacattca ctgtcagcta cactgaactg tctaacaaac ctggtttaaa gtaattcata 203640taaaacaaat aaag 203654 4 589 PRT HUMAN 4 Gly Ser Ala Asp Ser Tyr Thr SerArg Pro Ser Asp Ser Asp Val Ser 1 5 10 15 Leu Glu Glu Asp Arg Glu AlaVal Arg Arg Glu Ala Glu Arg Gln Ala 20 25 30 Gln Ala Gln Leu Glu Lys AlaLys Thr Lys Pro Val Ala Phe Ala Val 35 40 45 Arg Thr Asn Val Ser Tyr SerAla Ala His Glu Asp Asp Val Pro Val 50 55 60 Pro Gly Met Ala Ile Ser PheGlu Ala Lys Asp Phe Leu His Val Lys 65 70 75 80 Glu Lys Phe Asn Asn AspTrp Trp Ile Gly Arg Leu Val Lys Glu Gly 85 90 95 Cys Glu Ile Gly Phe IlePro Ser Pro Val Lys Leu Glu Asn Met Arg 100 105 110 Leu Gln His Glu GlnArg Ala Lys Gln Gly Lys Phe Tyr Ser Ser Lys 115 120 125 Ser Gly Gly AsnSer Ser Ser Ser Leu Gly Asp Ile Val Pro Ser Ser 130 135 140 Arg Lys SerThr Pro Pro Ser Ser Ala Ile Asp Ile Asp Ala Thr Gly 145 150 155 160 LeuAsp Ala Glu Glu Asn Asp Ile Pro Ala Asn His Arg Ser Pro Lys 165 170 175Pro Ser Ala Asn Ser Val Thr Ser Pro His Ser Lys Glu Lys Arg Met 180 185190 Pro Phe Phe Lys Lys Thr Glu His Thr Pro Pro Tyr Asp Val Val Pro 195200 205 Ser Met Arg Pro Val Val Leu Val Gly Pro Ser Leu Lys Gly Tyr Glu210 215 220 Val Thr Asp Met Met Gln Lys Ala Leu Phe Asp Phe Leu Lys HisArg 225 230 235 240 Phe Glu Gly Arg Ile Ser Ile Thr Arg Val Thr Ala AspIle Ser Leu 245 250 255 Ala Lys Arg Ser Val Leu Asn Asn Pro Ser Lys HisAla Ile Ile Glu 260 265 270 Arg Ser Asn Thr Arg Ser Ser Leu Ala Glu ValGln Ser Glu Ile Glu 275 280 285 Arg Ile Phe Glu Leu Ala Arg Thr Leu GlnLeu Val Val Leu Asp Ala 290 295 300 Asp Thr Ile Asn His Pro Ala Gln LeuSer Lys Thr Ser Leu Ala Pro 305 310 315 320 Ile Ile Val Tyr Val Lys IleSer Ser Pro Lys Val Leu Gln Arg Leu 325 330 335 Ile Lys Ser Arg Gly LysSer Gln Ala Lys His Leu Asn Val Gln Met 340 345 350 Val Ala Ala Asp LysLeu Ala Gln Cys Pro Pro Glu Leu Phe Asp Val 355 360 365 Ile Leu Asp GluAsn Gln Leu Glu Asp Ala Cys Glu His Leu Ala Asp 370 375 380 Tyr Leu GluAla Tyr Trp Lys Ala Thr His Pro Pro Ser Ser Ser Leu 385 390 395 400 ProAsn Pro Leu Leu Ser Arg Thr Leu Ala Thr Ser Ser Leu Pro Leu 405 410 415Ser Pro Thr Leu Ala Ser Asn Ser Gln Gly Ser Gln Gly Asp Gln Arg 420 425430 Thr Asp Arg Ser Ala Pro Ile Arg Ser Ala Ser Gln Ala Glu Glu Glu 435440 445 Pro Ser Val Glu Pro Val Lys Lys Ser Gln His Arg Ser Ser Ser Ser450 455 460 Ala Pro His His Asn His Arg Ser Gly Thr Ser Arg Gly Leu SerArg 465 470 475 480 Gln Glu Thr Phe Asp Ser Glu Thr Gln Glu Ser Arg AspSer Ala Tyr 485 490 495 Val Glu Pro Lys Glu Asp Tyr Ser His Asp His ValAsp His Tyr Ala 500 505 510 Ser His Arg Asp His Asn His Arg Asp Glu ThrHis Gly Ser Ser Asp 515 520 525 His Arg His Arg Glu Ser Arg His Arg SerArg Asp Val Asp Arg Glu 530 535 540 Gln Asp His Asn Glu Cys Asn Lys GlnArg Ser Arg His Lys Ser Lys 545 550 555 560 Asp Arg Tyr Cys Glu Lys AspGly Glu Val Ile Ser Lys Lys Arg Asn 565 570 575 Glu Ala Gly Glu Trp AsnArg Asp Val Tyr Ile Pro Gln 580 585 5 606 PRT HUMAN 5 Met Leu Asp ArgHis Leu Ala Ala Pro His Thr Gln Gly Leu Val Leu 1 5 10 15 Glu Gly SerAla Asp Ser Tyr Thr Ser Arg Pro Ser Asp Ser Asp Val 20 25 30 Ser Leu GluGlu Asp Arg Glu Ala Val Arg Arg Glu Ala Glu Arg Gln 35 40 45 Ala Gln AlaGln Leu Glu Lys Ala Lys Thr Lys Pro Val Ala Phe Ala 50 55 60 Val Arg ThrAsn Val Ser Tyr Ser Ala Ala His Glu Asp Asp Val Pro 65 70 75 80 Val ProGly Met Ala Ile Ser Phe Glu Ala Lys Asp Phe Leu His Val 85 90 95 Lys GluLys Phe Asn Asn Asp Trp Trp Ile Gly Arg Leu Val Lys Glu 100 105 110 GlyCys Glu Ile Gly Phe Ile Pro Ser Pro Val Lys Leu Glu Asn Met 115 120 125Arg Leu Gln His Glu Gln Arg Ala Lys Gln Gly Lys Phe Tyr Ser Ser 130 135140 Lys Ser Gly Gly Asn Ser Ser Ser Ser Leu Gly Asp Ile Val Pro Ser 145150 155 160 Ser Arg Lys Ser Thr Pro Pro Ser Ser Ala Ile Asp Ile Asp AlaThr 165 170 175 Gly Leu Asp Ala Glu Glu Asn Asp Ile Pro Ala Asn His ArgSer Pro 180 185 190 Lys Pro Ser Ala Asn Ser Val Thr Ser Pro His Ser LysGlu Lys Arg 195 200 205 Met Pro Phe Phe Lys Lys Thr Glu His Thr Pro ProTyr Asp Val Val 210 215 220 Pro Ser Met Arg Pro Val Val Leu Val Gly ProSer Leu Lys Gly Tyr 225 230 235 240 Glu Val Thr Asp Met Met Gln Lys AlaLeu Phe Asp Phe Leu Lys His 245 250 255 Arg Phe Glu Gly Arg Ile Ser IleThr Arg Val Thr Ala Asp Ile Ser 260 265 270 Leu Ala Lys Arg Ser Val LeuAsn Asn Pro Ser Lys His Ala Ile Ile 275 280 285 Glu Arg Ser Asn Thr ArgSer Ser Leu Ala Glu Val Gln Ser Glu Ile 290 295 300 Glu Arg Ile Phe GluLeu Ala Arg Thr Leu Gln Leu Val Val Leu Asp 305 310 315 320 Ala Asp ThrIle Asn His Pro Ala Gln Leu Ser Lys Thr Ser Leu Ala 325 330 335 Pro IleVal Val Tyr Val Lys Ile Ser Ser Pro Lys Val Leu Gln Arg 340 345 350 LeuIle Lys Ser Arg Gly Lys Ser Gln Ala Lys His Leu Asn Val Gln 355 360 365Met Val Ala Ala Asp Lys Leu Ala Gln Cys Pro Pro Glu Leu Phe Asp 370 375380 Val Ile Leu Asp Glu Asn Gln Leu Glu Asp Ala Cys Glu His Leu Ala 385390 395 400 Asp Tyr Leu Glu Ala Tyr Trp Lys Ala Thr His Pro Pro Ser SerAsn 405 410 415 Leu Pro Asn Pro Leu Leu Ser Arg Thr Leu Ala Thr Ser AlaLeu Pro 420 425 430 Val Ser Pro Thr Leu Ala Ser Asn Ser Gln Gly Ser GlnGly Asp Gln 435 440 445 Arg Thr Asp Arg Ser Ala Pro Ala Arg Ser Ala SerGln Ala Glu Glu 450 455 460 Glu Pro Cys Leu Glu Pro Ala Lys Lys Ser GlnHis Arg Ser Ser Ser 465 470 475 480 Ser Ala Pro His His Asn His Arg SerGly Thr Ser Arg Gly Leu Ser 485 490 495 Arg Gln Glu Thr Phe Asp Ser GluThr Gln Glu Ser Arg Asp Ser Ala 500 505 510 Tyr Val Glu Pro Lys Glu AspTyr Ser His Glu His Val Asp His Tyr 515 520 525 Ala Pro His Arg Asp HisAsn His Arg Asp Glu Thr His Arg Ser Ser 530 535 540 Asp His Arg His ArgGlu Thr Arg His Arg Ser Arg Asp Met Asp Arg 545 550 555 560 Glu Gln AspHis Asn Glu Cys Asn Lys Gln Arg Ser Arg His Lys Ser 565 570 575 Lys AspArg Tyr Cys Asp Lys Asp Gly Glu Val Ile Ser Lys Lys Arg 580 585 590 AsnGlu Ala Gly Glu Trp Asn Arg Asp Val Tyr Ile Arg Gln 595 600 605

That which is claimed is:
 1. An isolated peptide consisting of an aminoacid sequence selected from the group consisting of: (a) an amino acidsequence shown in SEQ ID NO:2; (b) an amino acid sequence of an allelicvariant of an amino acid sequence shown in SEQ ID NO:2, wherein saidallelic variant is encoded by a nucleic acid molecule that hybridizesunder stringent conditions to the opposite strand of a nucleic acidmolecule shown in SEQ ID NOS:1 or 3; (c) an amino acid sequence of anortholog of an amino acid sequence shown in SEQ ID NO:2, wherein saidortholog is encoded by a nucleic acid molecule that hybridizes understringent conditions to the opposite strand of a nucleic acid moleculeshown in SEQ ID NOS:1 or 3; and (d) a fragment of an amino acid sequenceshown in SEQ ID NO:2, wherein said fragment comprises at least 10contiguous amino acids.
 2. An isolated peptide comprising an amino acidsequence selected from the group consisting of: (a) an amino acidsequence shown in SEQ ID NO:2; (b) an amino acid sequence of an allelicvariant of an amino acid sequence shown in SEQ ID NO:2, wherein saidallelic variant is encoded by a nucleic acid molecule that hybridizesunder stringent conditions to the opposite strand of a nucleic acidmolecule shown in SEQ ID NOS:1 or 3; (c) an amino acid sequence of anortholog of an amino acid sequence shown in SEQ ID NO:2, wherein saidortholog is encoded by a nucleic acid molecule that hybridizes understringent conditions to the opposite strand of a nucleic acid moleculeshown in SEQ ID NOS:1 or 3; and (d) a fragment of an amino acid sequenceshown in SEQ ID NO:2, wherein said fragment comprises at least 10contiguous amino acids.
 3. An isolated antibody that selectively bindsto a peptide of claim
 2. 4. An isolated nucleic acid molecule consistingof a nucleotide sequence selected from the group consisting of: (a) anucleotide sequence that encodes an amino acid sequence shown in SEQ IDNO:2; (b) a nucleotide sequence that encodes of an allelic variant of anamino acid sequence shown in SEQ ID NO:2, wherein said nucleotidesequence hybridizes under stringent conditions to the opposite strand ofa nucleic acid molecule shown in SEQ ID NOS:1 or 3; (c) a nucleotidesequence that encodes an ortholog of an amino acid sequence shown in SEQID NO:2, wherein said nucleotide sequence hybridizes under stringentconditions to the opposite strand of a nucleic acid molecule shown inSEQ ID NOS:1 or 3; (d) a nucleotide sequence that encodes a fragment ofan amino acid sequence shown in SEQ ID NO:2, wherein said fragmentcomprises at least 10 contiguous amino acids; and (e) a nucleotidesequence that is the complement of a nucleotide sequence of (a)-(d). 5.An isolated nucleic acid molecule comprising a nucleotide sequenceselected from the group consisting of: (a) a nucleotide sequence thatencodes an amino acid sequence shown in SEQ ID NO:2; (b) a nucleotidesequence that encodes of an allelic variant of an amino acid sequenceshown in SEQ ID NO:2, wherein said nucleotide sequence hybridizes understringent conditions to the opposite strand of a nucleic acid moleculeshown in SEQ ID NOS:1 or 3; (c) a nucleotide sequence that encodes anortholog of an amino acid sequence shown in SEQ ID NO:2, wherein saidnucleotide sequence hybridizes under stringent conditions to theopposite strand of a nucleic acid molecule shown in SEQ ID NOS:1 or 3;(d) a nucleotide sequence that encodes a fragment of an amino acidsequence shown in SEQ ID NO:2, wherein said fragment comprises at least10 contiguous amino acids; and (e) a nucleotide sequence that is thecomplement of a nucleotide sequence of (a)-(d).
 6. A gene chipcomprising a nucleic acid molecule of claim
 5. 7. A transgenic non-humananimal comprising a nucleic acid molecule of claim
 5. 8. A nucleic acidvector comprising a nucleic acid molecule of claim
 5. 9. A host cellcontaining the vector of claim
 8. 10. A method for producing any of thepeptides of claim 1 comprising introducing a nucleotide sequenceencoding any of the amino acid sequences in (a)-(d) into a host cell,and culturing the host cell under conditions in which the peptides areexpressed from the nucleotide sequence.
 11. A method for producing anyof the peptides of claim 2 comprising introducing a nucleotide sequenceencoding any of the amino acid sequences in (a)-(d) into a host cell,and culturing the host cell under conditions in which the peptides areexpressed from the nucleotide sequence.
 12. A method for detecting thepresence of any of the peptides of claim 2 in a sample, said methodcomprising contacting said sample with a detection agent thatspecifically allows detection of the presence of the peptide in thesample and then detecting the presence of the peptide.
 13. A method fordetecting the presence of a nucleic acid molecule of claim 5 in asample, said method comprising contacting the sample with anoligonucleotide that hybridizes to said nucleic acid molecule understringent conditions and determining whether the oligonucleotide bindsto said nucleic acid molecule in the sample.
 14. A method foridentifying a modulator of a peptide of claim 2, said method comprisingcontacting said peptide with an agent and determining if said agent hasmodulated the function or activity of said peptide.
 15. The method ofclaim 14, wherein said agent is administered to a host cell comprisingan expression vector that expresses said peptide.
 16. A method foridentifying an agent that binds to any of the peptides of claim 2, saidmethod comprising contacting the peptide with an agent and assaying thecontacted mixture to determine whether a complex is formed with theagent bound to the peptide.
 17. A pharmaceutical composition comprisingan agent identified by the method of claim 16 and a pharmaceuticallyacceptable carrier therefor.
 18. A method for treating a disease orcondition mediated by a human transporter protein, said methodcomprising administering to a patient a pharmaceutically effectiveamount of an agent identified by the method of claim
 16. 19. A methodfor identifying a modulator of the expression of a peptide of claim 2,said method comprising contacting a cell expressing said peptide with anagent, and determining if said agent has modulated the expression ofsaid peptide.
 20. An isolated human transporter peptide having an aminoacid sequence that shares at least 70% homology with an amino acidsequence shown in SEQ ID NO:2.
 21. A peptide according to claim 20 thatshares at least 90 percent homology with an amino acid sequence shown inSEQ ID NO:2.
 22. An isolated nucleic acid molecule encoding a humantransporter peptide, said nucleic acid molecule sharing at least 80percent homology with a nucleic acid molecule shown in SEQ ID NOS:1 or3.
 23. A nucleic acid molecule according to claim 22 that shares atleast 90 percent homology with a nucleic acid molecule shown in SEQ IDNOS:1 or 3.